Microbial degradation of estrogens using activated sludge and night soil-composting microorganisms

2004 ◽  
Vol 50 (8) ◽  
pp. 153-159 ◽  
Author(s):  
J.H. Shi ◽  
Y. Suzuki ◽  
S. Nakai ◽  
M. Hosomi
Keyword(s):  
Activated Sludge ◽  
Microbial Degradation ◽  
Estrogenic Activity ◽  
Degradation Products ◽  
Order Reaction ◽  
Yeast Two Hybrid ◽  
First Order ◽  
Natural Estrogens ◽  
17Β Estradiol ◽  
Two Hybrid

In order to investigate the potential for microbial degradation of estrogens, and the products formed, activated sludge collected from Korea (ASK) and night soil-composting microorganisms (NSCM) were used to degrade estrogens. Results showed that both ASK and NSCM degraded almost 100% of the natural estrogens estrone (E1), 17β-estradiol (E2), and estriol (E3) from initial concentrations of 20-25 mg/L, while synthetic estrogen, ethynylestradiol (EE2), was not degraded. Analysis of degradation products of E2 by using HPLC-ECD and a consecutive first-order reaction calculation confirmed that E2 was sequentially degraded to E1, which was further degraded to other unknown compounds by ASK and NSCM. We then used the yeast two-hybrid assay to show that the unknown degradation products did not appear to possess estrogenic activity when E1, E2 or E3 were degraded to below the detection limit after 14 days of incubation, indicating that ASK and NSCM not only degrade natural estrogens, but also remove their estrogenic activities.

Determination of estrogenic activity in landfill leachate by simplified yeast two-hybrid assay

2002 ◽  
Vol 4 (6) ◽  
pp. 1040-1046 ◽  
Author(s):  
Yasunori Kawagoshi ◽  
Yukiko Tsukagoshi ◽  
Isao Fukunaga
Keyword(s):  
Landfill Leachate ◽  
Estrogenic Activity ◽  
Yeast Two Hybrid ◽  
Two Hybrid

Assessing Estrogenic Activities of Selected Endocrine Disrupting Chemicals and their Combination Effects

2013 ◽  
Vol 765-767 ◽  
pp. 2944-2948 ◽  
Author(s):  
Xiao Ling Shao ◽  
Wen Qi Zhong ◽  
Xiao Yan Ma ◽  
Ang Gao ◽  
Xiang Yang Wu ◽  
...  
Keyword(s):  
Binary Systems ◽  
Endocrine Disrupting Chemicals ◽  
Concentration Addition ◽  
Multicomponent Mixtures ◽  
Yeast Two Hybrid ◽  
Yeast Two Hybrid System ◽  
Endocrine Disrupting ◽  
17Β Estradiol ◽  
Ethylhexyl Phthalate ◽  
Two Hybrid

Yeast two-hybrid system was used to investigate the estrogenic activities of 13 kinds of representative endocrine disrupting chemicals (EDCs) and their combinary effects. Results show that the order of estrogenic potencies for these chemicals is: 17α-ethynylestradiol>diethylstilbestrol >17β-estradiol>estrone>estriol>branchedp-nonylphenol>4-t-octylphenol>bisphenol A>diethyl phthalate>4-n-nonylphenol>di-(2-ethylhexyl) phthalate>dibutyl phthalate>dimethyl phthalate. The mixture effects of multiple EDCs were compared to those obtained from individual chemicals, using the model of concentration addition. Results reveal that the estrogenicities of multicomponent mixtures of more than three (including three) of EDCs follow antagonistic effects, while there is no definite conclusion for binary systems. The less than additive effects were also confirmed in the spiked experiments conducted in the extracts of real water samples.

Kinetics of polymeric substrate (dextrin or peptone) removal by activated sludge: hydrolysis of polymers to monomers is the rate-determining step

1997 ◽  
Vol 36 (12) ◽  
pp. 159-167 ◽  
Author(s):  
Yuu Ubukata
Keyword(s):  
Amino Acids ◽  
Activated Sludge ◽  
Reaction Kinetics ◽  
Removal Rate ◽  
Order Reaction ◽  
First Order Reaction ◽  
Municipal Sewage ◽  
First Order ◽  
Rate Determining Step ◽  
Hydrolysis Of

Most of the organic compounds in primary effluent are polymers such as proteins and polysaccharides. However the bacteria present in activated sludge (AS) can only directly take up monomers such as amino acids and glucose which are produced from polymers by hydrolysis. Therefore, it is assumed that the hydrolysis of polymers to monomers by the bacteria is the rate-determining step in polymer removal. In this study, AS was acclimated to dextrin or peptone, and polymers (dextrin or peptone) and monomers (glucose or a mixture of free amino acids) were used as substrates for kinetic tests. The removal of monomers and polymers by the AS followed zero- and pseudo first-order reaction kinetics, respectively. The removal rate of monomers was higher than that of polymers, and the oxygen uptake rate of the AS during monomer removal was higher than that during polymer removal. One of the important differences between the polymers and monomers used in this study is whether glycosidic linkages or peptide bonds exist in the material. It was therefore verified that the hydrolysis of polymers to monomers by AS was the rate-determining step in polymer removal. The removal of polymers apparently followed first-order reaction kinetics at high F/M ratios, but nth-order reaction kinetics at low F/M ratios (n>1), which are commonly used in municipal sewage treatment.

scholarly journals Concentration and Inhibitor Removal Methods for Determination of Estrogenic Activity in River Water using Yeast Two-Hybrid Test

2004 ◽  
Vol 27 (11) ◽  
pp. 679-684 ◽  
Author(s):  
Masahiro HOSOKAWA ◽  
Rui LIU ◽  
Takashi KAMEYA ◽  
Takashi KUBO ◽  
Kohei URANO
Keyword(s):  
River Water ◽  
Estrogenic Activity ◽  
Yeast Two Hybrid ◽  
Hybrid Test ◽  
Two Hybrid

scholarly journals Estrogenic Activity of Branched 4-Nonylphenol Isomers Examined by Yeast Two-Hybrid Assay

2006 ◽  
Vol 52 (2) ◽  
pp. 132-141 ◽  
Author(s):  
Hirotaka Shioji ◽  
Shinji Tsunoi ◽  
Yosuke Kobayashi ◽  
Tatsushi Shigemori ◽  
Michihiko Ike ◽  
...  
Keyword(s):  
Estrogenic Activity ◽  
Yeast Two Hybrid ◽  
Two Hybrid

Research on 17β-Estradiol Degradation Bacteria Isolated from Activated Sludge and its Degrade Characteristics

2011 ◽  
Vol 356-360 ◽  
pp. 2606-2609
Author(s):  
Li Zhang ◽  
Cui Cui Li
Keyword(s):  
Activated Sludge ◽  
Metal Ions ◽  
Bacterial Growth ◽  
Degradation Rate ◽  
Estrogenic Activity ◽  
Degradation Process ◽  
Initial Concentration ◽  
Low Concentrations ◽  
Negative Effect ◽  
17Β Estradiol

17β-estradiol(E2) has estrogenic activity at very low concentrations and are emerging as a major concern for water quality. Great endeavors have been done on the removal of E2 in wastewater. This article was mainly researched the isolated of E2 degradation bacteria from activated sludge and its degradation characteristics of the bacteria were also researched. According to its physiological biochemical results analysis, this strain was identified as K.pnem. pneumoniae .This strain can use E2 as sole carbon and energy source for growth. The optimal temperature, pH for the bacterial growth and degradation of E2 was 30°C,7.0, respectively, meanwhile degradation rate reached to 86% and 87%; degradation rate and bacterial growth increased along with E2 concentration increasing, 81% E2 was degraded when E2 concentration is 30mg/L, degradation rate decreased when E2>50mg/L; metal ions such as Fe2+and Zn2+almost have no effect on E2 degradation and bacterial growth; Mn2+can promote growth of strain and degradation, while, metal ions such as Hg2+,Ag+,Cu2+have negative effect on bacterial growth and degradation. The degradation process for E2 with initial concentration of 1mg/L indicated that the degradation rate of E2 by strain within 7days was 98%.

scholarly journals Heptachlor degradation characteristics of a novel strain and its application

2018 ◽  
Vol 77 (8) ◽  
pp. 2113-2122
Author(s):  
Liping Qiu ◽  
Hu Wang ◽  
Xuntao Wang
Keyword(s):  
Gene Sequence ◽  
Degradation Products ◽  
Carbon Sources ◽  
Reaction Model ◽  
Order Reaction ◽  
Ribosomal Database Project ◽  
16S Rdna Gene ◽  
Practical Applications ◽  
First Order ◽  
Degrading Microorganism

Abstract With heptachlor as the sole carbon source, an effective heptachlor-degrading microorganism (named strain H) was isolated from the sludge of heptachlor-polluted sewage of a chemical plant, via enrichment, screening and purification. Strain H was identified as a facultative anaerobic Gram-negative bacterial strain belonging to genus Shigella based on the physiological-biochemical characteristics and the similarity analysis of its 16S rDNA gene sequence with the sequences logged in the Ribosomal Database Project and GenBank databases. When the optimal inoculation volume and the pH were 20% and 7.1–7.6, respectively, strain H was able to degrade heptachlor by more than 88.2% after130 h, with initial concentration of heptachlor being 300 μg/L at 30 ± 0.5 °C. It was also shown that strain H can grow on the degradation products of heptachlor such as 1-hydroxychlordene or heptachlor epoxide. Furthermore, additional carbon sources can accelerate the degradation rate of heptachlor because of co-metabolism. The degradation dynamics could be described by a first-order reaction model. A real-world field experiment demonstrated that strain H was effective in practical applications of heptachlor biodegradation in contaminated soil.

scholarly journals Estrogenic Activity of Coffee Constituents

Nutrients ◽  
2019 ◽  
Vol 11 (6) ◽  
pp. 1401 ◽  
Author(s):  
Ryoiti Kiyama
Keyword(s):  
Cell Signaling ◽  
Binding Assay ◽  
Estrogenic Activity ◽  
Yeast Two Hybrid ◽  
Nitrogenous Compounds ◽  
Protein Assay ◽  
Gene Assay ◽  
Bone Protection ◽  
Signal Crosstalk ◽  
Two Hybrid

Here, the constituents of coffee with estrogenic activity are summarized by a comprehensive literature search, and their mechanisms of action for their physiological effects are discussed at the molecular and cellular levels. The estrogenic activity of coffee constituents, such as acids, caramelized products, carbohydrates, lignin, minerals, nitrogenous compounds, oil (lipids), and others, such as volatile compounds, was first evaluated by activity assays, such as animal tests, cell assay, ligand-binding assay, protein assay, reporter-gene assay, transcription assay, and yeast two-hybrid assay. Second, the health benefits associated with the estrogenic coffee constituents, such as bone protection, cancer treatment/prevention, cardioprotection, neuroprotection, and the improvement of menopausal syndromes, were summarized, including their potential therapeutic/clinical applications. Inconsistent results regarding mixed estrogenic/anti-estrogenic/non-estrogenic or biphasic activity, and unbeneficial effects associated with the constituents, such as endocrine disruption, increase the complexity of the effects of estrogenic coffee constituents. However, as the increase of the knowledge about estrogenic cell signaling, such as the types of specific signaling pathways, selective modulations of cell signaling, signal crosstalk, and intercellular/intracellular networks, pathway-based assessment will become a more realistic means in the future to more reliably evaluate the beneficial applications of estrogenic coffee constituents.

Sign in / Sign up

Export Citation Format

Share Document