scholarly journals Morphological and molecular identification of Phytophthora cinnamomi Rands as causal agent of Crown and root rot in Blueberry (Vaccinium corymbosum) in Peru

2018 ◽  
Vol 2 (2) ◽  
pp. 14
Author(s):  
M. Huarhua ◽  
J. Flores ◽  
R. Acuña ◽  
W. Apaza
2016 ◽  
Vol 164 (11-12) ◽  
pp. 959-966 ◽  
Author(s):  
Laura Luongo ◽  
Salvatore Vitale ◽  
Massimo Galli ◽  
Anita Haegi ◽  
Stefan Wagner ◽  
...  

HortScience ◽  
1999 ◽  
Vol 34 (4) ◽  
pp. 693-695 ◽  
Author(s):  
Amal de Silva ◽  
Keith Patterson ◽  
Craig Rothrock ◽  
Ron McNew

Phytophthora root rot is a severe disease on blueberry (Vaccinium corymbosum L.) in poorly drained soils. Little is known about how mulching and frequent waterlogging affect disease severity in blueberries. Phytophthora cinnamomi Rands was grown on rice hulls, which were incorporated into the soil at the rate of 10% (v:v). Waterlogging conditions were imposed for 48 hours 1 week after planting on mulched and nonmulched blueberry plants at weekly, biweekly, and monthly intervals for a total of 3 months. Control plants were not subjected to flooding. The severity of Phytophthora root rot increased with time. Significant linear relationships were found between flooding interval and disease severity rating of shoot, percentage of root infection, and shoot and root dry weights of plants. Disease symptoms were minimal in control plants, but shoot disease rating and percentage of root infection were high in mulched and nonmulched plants that were flooded every week. Shoot and root dry weights were higher in 1997 than in 1996. In 1996, mulched plants had higher shoot dry weights than did nonmulched plants. Disease incidence was higher with weekly and biweekly flooding than with monthly or no flooding. However, mulching did not affect root infection.


HortScience ◽  
2007 ◽  
Vol 42 (6) ◽  
pp. 1463-1467 ◽  
Author(s):  
David R. Bryla ◽  
Robert G. Linderman

A 2-year study was done in Oregon to determine the effects of irrigation method and level of water application on the development of root rot in northern highbush blueberry (Vaccinium corymbosum L. ‘Duke’). Plants were grown on mulched, raised beds and irrigated by overhead sprinklers, microsprays, or drip at 50%, 100%, and 150% of the estimated crop evapotranspiration requirement. Soil at the site was a silty clay loam. By the end of the first season, plants were largest with drip, intermediate-sized with microsprays and smallest with sprinklers; however, this was not the case the next season. By the end of year 2, plants irrigated by drip had less canopy cover, fewer new canes, lower pruning weights, and only half the shoot and root dry weight as plants irrigated by sprinklers or microsprays. Destructive sampling revealed that the field was infested by root rot. Less growth with drip was association with higher levels of infection by the root pathogen, Phytophthora cinnamomi. Phytophthora infection increased with water application, regardless of irrigation method, but averaged 14% with drip and only 7% with sprinklers and microsprays. Roots were also infected by Pythium spp. Pythium infection likewise increased with the total amount of water applied but, unlike P. cinnamomi, was similar among irrigation methods. Overall, drip irrigation maintained higher soil water content near the base of the plants than sprinklers and microsprays, resulting in conditions more favorable to root rot. Sprinklers and microsprays may be better alternatives than drip at sites prone to problems with the disease.


Plant Disease ◽  
2020 ◽  
Vol 104 (12) ◽  
pp. 3269
Author(s):  
L. Sigillo ◽  
C. Pane ◽  
I. Garaguso ◽  
L. Luongo ◽  
M. Galli ◽  
...  

Plant Disease ◽  
2020 ◽  
Author(s):  
Milan Panth ◽  
Fulya Baysal-Gurel ◽  
Farhat A. Avin ◽  
Terri Simmons

Soilborne diseases caused by pathogens such as Phytophthora, Rhizoctonia, Fusarium, Verticillium, and Pythium species are the most important diseases of woody ornamentals. Ginkgo (Ginkgo biloba) and red maple (Acer rubrum) ‘October Glory’ plants grown in containers and fields in Tennessee have shown root and crown rot symptoms with dark brown to black lesions in 2017 and 2018. The objective of this research was to isolate and identify pathogens affecting ginkgo and red maple plants in nurseries of Tennessee and develop fungicide/biofungicide management recommendations for nursery producers. Isolations were made from the infected roots. Several Phytophthora-like colonies with spherical zoospores, filamentous to globose oogoni, and whitish mycelium, were isolated on V8-PARPH medium. For confirming identity, total genomic DNA was extracted followed by the sequence analysis of the internal transcribed spacer (ITS) regions, and large subunit (LSU) of the nuclear ribosomal RNA (rRNA) as well as cytochrome c oxidase subunit I (Cox I) and cytochrome c oxidase subunit II (Cox II) of mitochondrial DNA (mtDNA). Based on morphological and molecular analysis, Phytopythium vexans was described as a causal agent of crown and root rot from the infected ginkgo and red maple plants. To complete Koch’s postulates, a pathogenicity test was performed by drenching 100 ml V8 agar medium slurry of P. vexans inoculum on 1-year-old potted ginkgo plant root systems as well as red maple ‘October Glory’. Necrotic lesion development was observed in the root system 45 days after inoculation and P. vexans was re-isolated from the roots of both ginkgo and red maple. All control ginkgo and red maple plants remained disease-free and no pathogen was re-isolated. In addition, the efficacy of fungicides, biofungicides, fertilizer and host-plant defense inducers (traditionally recommended for management of oomycete diseases) for control of Phytopythium crown and root rot was evaluated on ginkgo and red maple ‘October Glory’ seedlings in greenhouse and field trials. The fungicides such as Empress Intrinsic, Pageant Intrinsic, Segovis and Subdue MAXX were effective in both greenhouse and field trials, and the biofungicide Stargus reduced the disease severity caused by pathogen P. vexans on ginkgo and red maple plants in greenhouse trials. These results will help nursery producers to make proper management decisions for newly reported Phytopythium crown and root rot disease of ginkgo and red maple plants.


2019 ◽  
Vol 102 (1) ◽  
pp. 143-154
Author(s):  
Saeedeh Dehghanpour-Farashah ◽  
Parissa Taheri ◽  
Mahrokh Falahati-Rastegar

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