The Ability of Blood Plasma to Inhibit Catalase in the Presence of Chloride is a Highly Sensitive Indicator of Deposited Nitric Oxide and Leukocyte Activation

2020 ◽  
Vol 16 (2) ◽  
pp. 172-180
Author(s):  
Vladimir Titov ◽  
Anatoly Osipov ◽  
Anatoly Vanin
Keyword(s):  
Nitric Oxide ◽  
Blood Plasma ◽  
Inflammatory Diseases ◽  
Clinical Signs ◽  
Iron Complexes ◽  
Sensitive Indicator ◽  
Specific Method ◽  
Leukocyte Activation ◽  
Highly Sensitive ◽  
Nitrosyl Iron Complexes

Aims: To find out the origin of so-called nitrite - like substance (NLS) that appears in the blood plasma in patients with inflammatory diseases and the mechanism of its occurrence. To justify the possibility of registering its appearance in the blood as a highly sensitive indicator of leukocyte activation. Background: The need for a simple, sensitive and specific method of early diagnosis of inflammation, the key stage of which is the activation of white blood cells. Objective: To find out the origin of so-called nitrite - like substance (NLS) that appears in the blood plasma in patients with inflammatory diseases before the onset of clinical signs. This substance is able to inhibit catalase in the presence of chloride which is typical for nitrite and nitrosoamines. Methods: The catalase activity was determined by the calorimetric method based on the control of the kinetics of heat production accompanying hydrogen peroxide decomposition. Results: Blood plasma contains deposited nitric oxide included in various nitrosyl iron complexes. These complexes effectively interact with the superoxide produced by activated leukocytes. This interaction produces a number of substances that have the ability to inhibit catalase in the presence of chloride. These substances retain the ability to inhibit in the system: hemoglobin-iron chelator, or hemoglobin-mercury salt. Such properties are characteristic of nitrite and nitrosoamines. Normally, these substances are present in plasma in trace amounts. 700 activated cells per microliter (10 times less than normal in human blood) are enough to transform about 30% nitrosyl iron complexes contained in plasma into NLS. Conclusion: The appearance of NLS is a very sensitive indicator of leukocyte activation.

Spectroscopic Description of the Two Nitrosyl−Iron Complexes Responsible for Fur Inhibition by Nitric Oxide

2004 ◽  
Vol 126 (19) ◽  
pp. 6005-6016 ◽  
Author(s):  
Benoît D'Autréaux ◽  
Olivier Horner ◽  
Jean-Louis Oddou ◽  
Claudine Jeandey ◽  
Serge Gambarelli ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Iron Complexes ◽  
Nitrosyl Iron Complexes

Nitric oxide, nitrosyl iron complexes, ferritin and frataxin: A well equipped team to preserve plant iron homeostasis

Plant Science ◽  
2011 ◽  
Vol 181 (5) ◽  
pp. 582-592 ◽  
Author(s):  
Leonor Ramirez ◽  
Marcela Simontacchi ◽  
Irene Murgia ◽  
Eduardo Zabaleta ◽  
Lorenzo Lamattina
Keyword(s):  
Nitric Oxide ◽  
Iron Homeostasis ◽  
Iron Complexes ◽  
Nitrosyl Iron Complexes

scholarly journals Thermal Liquid Biopsy (TLB) of Blood Plasma as a Potential Tool to Help in the Early Diagnosis of Multiple Sclerosis

2021 ◽  
Vol 11 (4) ◽  
pp. 295
Author(s):  
Ferdinanda Annesi ◽  
Sonia Hermoso-Durán ◽  
Bruno Rizzuti ◽  
Rosalinda Bruno ◽  
Domenico Pirritano ◽  
...  
Keyword(s):  
Multiple Sclerosis ◽  
Blood Plasma ◽  
Liquid Biopsy ◽  
Inflammatory Diseases ◽  
Early Stage ◽  
Objective Assessment ◽  
Clinical Signs ◽  
Test Methods ◽  
Diagnostic Methods ◽  
Performance Indexes

Background: Multiple sclerosis (MS) is frequently characterized by a variety of clinical signs, often exhibiting little specificity. The diagnosis requires a combination of medical observations and instrumental tests, and any support for its objective assessment is helpful. Objective: Herein, we describe the application of thermal liquid biopsy (TLB) of blood plasma samples, a methodology for predicting the occurrence of MS with a noninvasive, quick blood test. Methods: TLB allows one to define an index (TLB score), which provides information about overall real-time alterations in plasma proteome that may be indicative of MS. Results: This pilot study, based on 85 subjects (45 MS patients and 40 controls), showed good performance indexes (sensitivity and specificity both around 70%). The diagnostic methods better discriminate between early stage and low-burden MS patients, and it is not influenced by gender, age, or assumption of therapeutic drugs. TLB is more accurate for patients having low disability level (≤ 3.0, measured by the expanded disability status scale, EDSS) and a relapsing–remitting diagnosis. Conclusion: Our results suggest that TLB can be applied to MS, especially in an initial phase of the disease when diagnosis is difficult and yet more important (in such cases, accuracy of prediction is close to 80%), as well as in personalized patient periodic monitoring. The next step will be determining its utility in differentiating between MS and other disorders, in particular in inflammatory diseases.

Exposure to nitric oxide increases the nitrosyl-iron complexes content in sorghum embryonic axes

Plant Science ◽  
2012 ◽  
Vol 183 ◽  
pp. 159-166 ◽  
Author(s):  
Marcela Simontacchi ◽  
Agustina Buet ◽  
Lorenzo Lamattina ◽  
Susana Puntarulo
Keyword(s):  
Nitric Oxide ◽  
Iron Complexes ◽  
Embryonic Axes ◽  
Nitrosyl Iron Complexes

scholarly journals New ELISA System for Myeloid-related Protein Complex (MRP8/14) and Its Clinical Significance as a Sensitive Marker for Inflammatory Responses Associated with Transplant Rejection

2003 ◽  
Vol 49 (4) ◽  
pp. 594-600 ◽  
Author(s):  
Masaki Ikemoto ◽  
Tomoko Tanaka ◽  
Yuri Takai ◽  
Hiroshi Murayama ◽  
Koichi Tanaka ◽  
...  
Keyword(s):  
Protein Complex ◽  
Inflammatory Responses ◽  
Inflammatory Diseases ◽  
Transplant Rejection ◽  
Specific Method ◽  
Related Protein ◽  
Elisa System ◽  
Reactive Protein ◽  
Highly Sensitive ◽  
Sensitive Marker

Abstract Background: C-reactive protein (CRP), a useful marker for inflammatory diseases, is not always sensitive to inflammatory reaction in the liver or other tissues. The aim of this study was to develop a sensitive and specific method for detecting inflammatory responses associated with transplant rejection. Methods: We developed a new, highly sensitive ELISA system for the measurement of serum human myeloid-related protein complex (MRP8/14), using monoclonal antibodies against MRP8/14, and applied it to specimens obtained from patients undergoing small intestine or liver transplantation. Results: This assay could detect MRP8/14 concentrations as low as 2 μg/L. Within-run CVs were 3.7–6.1% and between-day CVs were 5.6–8.7% for MRP8/14 concentrations of 117-3300 μg/L. Mean recovery was 104% (range, 80–128%). We observed a marked increase in serum MRP8/14 postoperatively in most recipients of transplants, followed by an increase in CRP 1–7 days after the increase in the complex. The increase in serum MRP8/14 occurred simultaneously with permeation of lymphocytes into the transplanted tissues as a result of rejection of the graft tissues. Conclusions: Accurate measurement of serum MRP8/14 provides a useful clinical diagnostic method tool for detecting inflammation associated with rejection of transplanted tissues.

Kinetic regularities of erythrocyte hemolysis and hemoglobin oxidation under the action of sulfur-nitrosyl iron complexes as nitric oxide donors

2010 ◽  
Vol 59 (12) ◽  
pp. 2215-2218 ◽  
Author(s):  
N. I. Neshev ◽  
B. L. Psikha ◽  
E. M. Sokolova ◽  
N. A. Sanina ◽  
T. N. Rudneva ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Iron Complexes ◽  
Nitric Oxide Donors ◽  
Erythrocyte Hemolysis ◽  
Hemoglobin Oxidation ◽  
Nitrosyl Iron Complexes

scholarly journals Induction and stability of human Th17 cells require endogenous NOS2 and cGMP-dependent NO signaling

2013 ◽  
Vol 210 (7) ◽  
pp. 1433-1445 ◽  
Author(s):  
Nataša Obermajer ◽  
Jeffrey L. Wong ◽  
Robert P. Edwards ◽  
Kong Chen ◽  
Melanie Scott ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
T Cells ◽  
Cd4 T Cells ◽  
Th17 Cells ◽  
De Novo ◽  
Inflammatory Diseases ◽  
Suppressor Cells ◽  
Guanosine Monophosphate ◽  
No Production ◽  
No Signaling

Nitric oxide (NO) is a ubiquitous mediator of inflammation and immunity, involved in the pathogenesis and control of infectious diseases, autoimmunity, and cancer. We observed that the expression of nitric oxide synthase-2 (NOS2/iNOS) positively correlates with Th17 responses in patients with ovarian cancer (OvCa). Although high concentrations of exogenous NO indiscriminately suppress the proliferation and differentiation of Th1, Th2, and Th17 cells, the physiological NO concentrations produced by patients’ myeloid-derived suppressor cells (MDSCs) support the development of RORγt(Rorc)+IL-23R+IL-17+ Th17 cells. Moreover, the development of Th17 cells from naive-, memory-, or tumor-infiltrating CD4+ T cells, driven by IL-1β/IL-6/IL-23/NO-producing MDSCs or by recombinant cytokines (IL-1β/IL-6/IL-23), is associated with the induction of endogenous NOS2 and NO production, and critically depends on NOS2 activity and the canonical cyclic guanosine monophosphate (cGMP)–cGMP-dependent protein kinase (cGK) pathway of NO signaling within CD4+ T cells. Inhibition of NOS2 or cGMP–cGK signaling abolishes the de novo induction of Th17 cells and selectively suppresses IL-17 production by established Th17 cells isolated from OvCa patients. Our data indicate that, apart from its previously recognized role as an effector mediator of Th17-associated inflammation, NO is also critically required for the induction and stability of human Th17 responses, providing new targets to manipulate Th17 responses in cancer, autoimmunity, and inflammatory diseases.

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