Enzyme Inhibition by Peroxynitrite-Mediated Tyrosine Nitration and Thiol Oxidation

2007 ◽  
Vol 3 (2) ◽  
pp. 103-117 ◽  
Author(s):  
Andreas Daiber ◽  
Markus Bachschmid
Keyword(s):  
Enzyme Inhibition ◽  
Tyrosine Nitration ◽  
Thiol Oxidation

scholarly journals Protein Tyrosine Nitration and Thiol Oxidation by Peroxynitrite—Strategies to Prevent These Oxidative Modifications

2013 ◽  
Vol 14 (4) ◽  
pp. 7542-7570 ◽  
Author(s):  
Andreas Daiber ◽  
Steffen Daub ◽  
Markus Bachschmid ◽  
Stefan Schildknecht ◽  
Matthias Oelze ◽  
...  
Keyword(s):  
Tyrosine Nitration ◽  
Thiol Oxidation ◽  
Protein Tyrosine ◽  
Protein Tyrosine Nitration ◽  
Oxidative Modifications

Optimal Duration of the Preincubation Phase in Enzyme Inhibition Experiments

2020 ◽  
Author(s):  
Petr Kuzmic
Keyword(s):  
Enzyme Inhibition ◽  
Dose Response ◽  
Enzyme Inhibitor ◽  
Single Point ◽  
Association Rate ◽  
Weakly Bound ◽  
Inhibitor Complex ◽  
Dissociation Equilibrium ◽  
Optimal Duration ◽  
Algebraic Formula

This report describes an algebraic formula to calculate the optimal duration of the pre-incubation phase in enzyme-inhibition experiments, based on the assumed range of expected values for the dissociation equilibrium constant of the enzyme–inhibitor complex and for the bimolecular association rate constant. Three typical experimental scenarios are treated, namely, (1) single-point primary screening at relatively high inhibitor concentrations; (2) dose-response secondary screening of relatively weakly bound inhibitors; (3) dose-response screening of tightly-bound inhibitors.

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