Paracrine Mechanisms Involved in Mesenchymal Stem Cell Differentiation into Cardiomyocytes

Cardiovascular disease (CVD) is one of the world-wide healthcare problem that involves the heart or blood vessels. CVD includes myocardial infarction and coronary artery diseases (CAD). Dysfunctional myocardial cells are leading causes of low cardiac output or ventricular dysfunction after cardiac arrest and may contribute to the progression of CVD which could not generate new cardiomyocytes in human adult heart. The mesenchymal stem cells (MSCs) which are present in adult marrow can self-renew and have the capacity of differentiation into multiple types of cells including cardiomyocytes. Recent biochemical analyses greatly revealed that several regulators of MSCs, such as HGF, PDGF, Wnt, and Notch-1 signaling pathways have been shown to be involved in the proliferation and differentiation into cardiomyocytes. Preclinical studies are paving the way for further applications of MSCs in the repair of myocardial infarction. In this study, we discuss and summarize the paracrine mechanisms involved in MSCs differentiation into cardiomyocytes.

Download Full-text

In the presence of TGF-β1, Asperosaponin VI promotes human mesenchymal stem cell differentiation into nucleus pulposus like- cells

BMC Complementary Medicine and Therapies ◽

10.1186/s12906-020-03169-y ◽

2021 ◽

Vol 21 (1) ◽

Author(s):

Yong-tao Niu ◽

Lin Xie ◽

Rong-Rong Deng ◽

Xiao-yu Zhang

Keyword(s):

Stem Cell ◽

Mesenchymal Stem Cell ◽

Western Blot ◽

Nucleus Pulposus ◽

Stem Cell Differentiation ◽

Human Mesenchymal Stem Cell ◽

Western Blot Assay ◽

Mesenchymal Stem Cell Differentiation ◽

Dipsacus Asper ◽

Proliferation And Differentiation

Abstract Background The regeneration of nucleus pulposus (NP) cells is an effective method to prevent intervertebral disc degeneration (IVDD). In this study, we investigated the role of Asperosaponin VI (ASA VI), isolated from a traditional Chinese medicine (TCM), the root of Dipsacus asper Wall, in promoting human mesenchymal stem cell (HMSC) proliferation and differentiation into NP-like cells and explored the possible mechanism of action. Methods The effects of ASA VI on HMSC viability and proliferation were determined by the XTT method and EDU staining. Then, Real-time qPCR, immunocytochemistry and immunofluorescence assays were used to measure the effect of ASA VI on the expression of extracellular matrix (ECM) components, such as COL2A1, aggrecan, SOX9, KRT19, PAX1, and glycosaminoglycans (GAGs), in NP cells. In addition, Western blot assay was used to measure the expression of p-ERK1/2 and p-smad2/3. Results ASA VI was able to promote the proliferation and differentiation of HMSCs into NP-like cells, and the optimum concentration was 1 mg/L. Western blot assay indicated that the possible mechanism might be related to the activation of p-ERK1 / 2 and p-Smad2 / 3. Conclusions ASA VI can promote the proliferation and differentiation of HMSCs into NP-like cells, which can potentially be used as a treatment for IVDD.

Download Full-text

Asperosaponin VI promotes human mesenchymal stem cell differentiation into nucleus pulposus like- cells via up-regulation of ERK1/2 and Smad2/3 signaling pathways.

10.21203/rs.3.rs-27853/v1 ◽

2020 ◽

Author(s):

Yong-Tao Niu ◽

Lin Xie ◽

Rong-Rong Deng ◽

Xiao-Yu Zhang

Keyword(s):

Stem Cell ◽

Mesenchymal Stem Cell ◽

Western Blot ◽

Nucleus Pulposus ◽

Stem Cell Differentiation ◽

Type Ii Collagen ◽

Human Mesenchymal Stem Cell ◽

Nucleus Pulposus Cells ◽

Mesenchymal Stem Cell Differentiation ◽

Proliferation And Differentiation

Abstract Background At present, the regeneration of nucleus pulposus cells is an effective method to prevent intervertebral disc degeneration (IVDD). In this study, we investigated the role of Asperosaponin VI (ASA VI), isolated from a traditional Chinese medicine (TCM), the root of Dipsacus asper Wall, in promoting human mesenchymal stem cell (HMSC) proliferation and differentiation into nucleus pulposus like-cells and explored its possible mechanism of action. Methods First, the effects of ASA VI on HMSC vitality and proliferation were determined by the XTT method and EDU staining. Then, HMSCs were cultured with ASA VI. Real-time PCR, immunocytochemistry and immunofluorescence were used to measure the expression of extracellular matrix (ECM) components in nucleus pulposus cells, such as type II collagen(COL2A1), aggrecan, SOX9, KRT19, PAX1 and glycosaminoglycans (GAGs), and Western blot was used to investigate its potential mechanism. Results ASA VI could promote HMSC differentiation into nucleus pulposus like-cells, and its optimum concentration was 1 mg/L. The Western blot assays indicated that the possible mechanisms involved upregulating the expression of P-ERK1/2 and P-Smad2/3. Conclusions ASA VI can promote HMSC proliferation and differentiation into nucleus pulposus like-cells, which can be used as a potential treatment for IVDD.

Download Full-text