Assessments of Immunomodulatory and Inflammatory effects against Induction of Entamoeba histolytica (HM1 IMS strain) crude extract Antigen in Complete Freund’s Adjuvant Induced Rheumatoid Arthritis Female Wistar Rats

2015 ◽  
Vol 14 (2) ◽  
pp. 113-127
Author(s):  
Swati Bagde ◽  
Vinod Singh
Keyword(s):  
Rheumatoid Arthritis ◽  
Entamoeba Histolytica ◽  
Crude Extract ◽  
Wistar Rats ◽  
Complete Freund’S Adjuvant ◽  
Female Wistar Rats ◽  
Freund’S Adjuvant ◽  
Complete Freund's Adjuvant ◽  
Freund's Adjuvant

scholarly journals Radiological Analysis of Hind Paw Joint in Murine Rheumatoid Arthritis Model Treated Prophylactically Or Therapeutically with Cassia fistula versus Naproxen

2020 ◽  
Vol 34 (4) ◽  
pp. 58-63
Author(s):  
Hassan Farooq ◽  
Mariyam Iftikhar Piracha ◽  
Muhammad Usman ◽  
Rabia Tariq ◽  
Saadia Shahzad Alam
Keyword(s):  
Rheumatoid Arthritis ◽  
Complete Freund’S Adjuvant ◽  
Male Rats ◽  
X Rays ◽  
Cassia Fistula ◽  
Freund’S Adjuvant ◽  
Complete Freund's Adjuvant ◽  
Freund's Adjuvant ◽  
Animal House ◽  
Radiological Analysis

Introduction: Cassia fistula has anti-inflammatory activity. Rheumatoid arthritis (RA) is an autoimmune disease, causing inflammation and disability of the affected joints. It is mostly treated symptomatically with NSAIDs, which themselves have a lot of adverse effects. In this study the anti-arthritic effect of Cassia fistula versus naproxen has been observed radiologically. Aims & Objectives: The present study was radiological analysis of anti-arthritic effect of Cassia fistula compared to naproxen in Complete Freund’s Adjuvant (CFA) induced rat model of rheumatoid arthritis. Place and duration of study: This study was conducted at the Animal House of University of Veterinary and Animal Sciences, Lahore for a period of three months. Material & Methods: The study was carried out on 96 male rats divided into 12 groups of 8 rats each. Single 0.2ml dose of Complete Freund’s Adjuvant (CFA) was injected in the right hind paw of each rat in all groups except Group 1 (negative control). Group 2 was positive control. The prophylactic (3-7) and therapeutic (8- 12) groups were given naproxen (25mg/kg), anthraquinone extract (250 &500mg/kg) or methanolic extract (250 & 500mg/kg) of Cassia fistula orally BD on days 1,2 &3 (first dose preceding CFA injection by 30 minutes) and on days 9,10 &11 for therapeutic groups respectively. On day 15, x-rays of right ankle joints of all groups were taken as a confirmatory investigation on the final status of the arthritis. Results: Our results showed both anthraquinone and methanolic extracts of Cassia fistula have dose dependent (500˃250mg/dl) prophylactic and therapeutic anti-arthritic potential albeit lesser than naproxen 25mg/kg, to reduce the swelling of hind paw and deformity of interphalangeal joints on radiological examination. Conclusion: The efficacy of Cassia fistula makes it a potential weaker candidate to naproxen in prevention and treatment of rheumatoid arthritis.

scholarly journals Terminalia tomentosa Bark Ameliorates Inflammation and Arthritis in Carrageenan Induced Inflammatory Model and Freund’s Adjuvant-Induced Arthritis Model in Rats

2019 ◽  
Vol 2019 ◽  
pp. 1-11 ◽  
Author(s):  
Srinivasa Reddy Jitta ◽  
Prasanthi Daram ◽  
Karthik Gourishetti ◽  
C. S. Misra ◽  
Picheswara Rao Polu ◽  
...  
Keyword(s):  
Wistar Rats ◽  
Complete Freund’S Adjuvant ◽  
Inflammatory Activity ◽  
Aqueous Extracts ◽  
Adjuvant Induced Arthritis ◽  
Freund’S Adjuvant ◽  
Complete Freund's Adjuvant ◽  
Freund's Adjuvant ◽  
Anti Inflammatory

Terminalia tomentosa bark belongs to the family Combretaceae. The plant bark is astringent and useful in the treatment of ulcers, vata, fractures, hemorrhages, bronchitis, and diarrhea. Phytochemical investigation of T. tomentosa bark confirms the presence of flavonoids, polyphenols, and tannins. The plant has not been investigated for its anti-inflammatory and antiarthritic activity. The present study was undertaken to explore its possible anti-inflammatory and antiarthritic activity. Anti-inflammatory activity of alcoholic and aqueous extracts of the bark was assessed by in vivo methods. In vivo antiarthritic potential of the extracts was evaluated by Complete Freund’s Adjuvant (CFA) induced arthritis in Wistar rats. Our findings showed that the alcoholic and aqueous extracts exhibited anti-inflammatory activity at 500 mg/kg oral dose in carrageenan-induced hind paw edema and carrageenan-induced air pouch inflammation models. We also found alcoholic as well as aqueous extracts of the bark restored the altered blood and serum parameters caused by the Complete Freund’s Adjuvant-induced arthritis in Wistar rats. This study shows that the T. tomentosa bark extracts possess anti-inflammatory activity and have pronounced effects on adjuvant arthritis also. Future studies are necessary to provide deeper insight into the exact mechanism of the action of anti-inflammatory and antiarthritic activity of T. tomentosa.

Serological characterization of γM (19 S) and γG (7 S) rabbit antibodies in response to Entamoeba histolytica antigens

1968 ◽  
Vol 14 (12) ◽  
pp. 1317-1323 ◽  
Author(s):  
Z. Ali Khan ◽  
E. Meerovitch
Keyword(s):  
Entamoeba Histolytica ◽  
Primary Response ◽  
Complete Freund’S Adjuvant ◽  
Secondary Response ◽  
Antibody Activity ◽  
Serological Characterization ◽  
Freund’S Adjuvant ◽  
Complete Freund's Adjuvant ◽  
Freund's Adjuvant

Entamoeba histolytica (DKB strain) antigen mixed with an equal volume of complete Freund's adjuvant was injected at multiple sites into three rabbits. Primary response sera were drawn 1, 2, and 3 weeks after the first injection. Two injections of antigen were given in the fourth week, at 3-day intervals. The secondary response sera were drawn 5, 6, and 12 weeks after the beginning of immunization. A steady hemagglutination (HA) titer of 1:128 was obtained with all the three primary response sera, while the secondary response sera showed HA titers of 1:2000 to 1:8000. The 0.1 M 2-mercaptoethanol treatment of these sera reduced, but did not completely obliterate, their HA activity. The HA activity was detected in both the pooled γM and γG classes of immunoglobulins of the primary and secondary response sera; the immunoprecipitating activity was confined to the γG class. 2-Mercaptoethanol treatment completely destroyed the antibody activity of γM fractions without affecting that of the γG fraction. Repeated immunoelectrophoresis runs of the whole sera and pooled γM and γG fractions showed variable numbers of precipitin bands at the γG position but none at the γM and γA positions.

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