New Anti-inflammatory Triterpene Esters and Glycosides from Alstonia scholaris

2020 ◽  
Vol 19 (4) ◽  
pp. 370-386
Author(s):  
Nighat Sultana ◽  
Muhammad Saleem Qazi ◽  
Mustafa Kamal
Keyword(s):  
Ethanolic Extract ◽  
Two Dimensional ◽  
1H And 13C Nmr ◽  
Alstonia Scholaris ◽  
Aerial Parts ◽  
Nmr Data ◽  
Lupeol Acetate ◽  
Anti Inflammatory ◽  
Phytochemical Studies ◽  
Triterpene Esters

Background:: Phytochemical studies on the ethanolic extract of aerial parts of Alstonia scholaris lead to the isolation of two new triterpenoid of the lanostanetype, lanosta 5ene,24-ethyl-3-O-β-D-glucopyranoside (1), lanosta,5ene,24-ethyl-3-O-β-D-glucopyranosideester (2) and new ursane type triterpenoidmethylester, 12-ursene-2,3,18,19-tetrol,28 acetate (nighascholarene) (3), together with seven known triterpenes, betuline, triterpene of the lupane type, alstoprenyol (4), 3β-hydroxy-28-β-acetoxy-5-olea triterpene (5),α-amyrin acetate (6), α-amyrin (7), lupeol acetate (8), 3β-hydroxy-24-nor-urs-4,12,28-triene triterpene (9) and ursolic acid (l0). Methodology:: The triterpenoid structures of these colorless compounds were deduced from the 1H and 13C-NMR data, and in particular from the application of two-dimensional 1H, 13C correlation experiments as well as by comparison with reported literature data. Results and Conclusion: This study deals with isolation and structural elucidation of natural new triterpenoidesters and glycosides with anti-inflammatory activity.

scholarly journals Phytochemical Analysis of Podospermum and Scorzonera n-Hexane Extracts and the HPLC Quantitation of Triterpenes

Molecules ◽  
2018 ◽  
Vol 23 (7) ◽  
pp. 1813 ◽  
Author(s):  
Özlem Bahadır-Acıkara ◽  
Serkan Özbilgin ◽  
Gülcin Saltan-İşcan ◽  
Stefano Dall’Acqua ◽  
Veronika Rjašková ◽  
...  
Keyword(s):  
Hplc Method ◽  
Phytochemical Analysis ◽  
Qualitative And Quantitative Analysis ◽  
Qualitative And Quantitative ◽  
Aerial Parts ◽  
Hexane Extracts ◽  
Lupeol Acetate ◽  
Anti Inflammatory

Previously tested n-hexane extracts of the Scorzonera latifolia showed promising bioactivity in vivo. Because triterpenes could account for this activity, n-hexane extracts were analyzed by HPLC to identify and quantify the triterpenes as the most abundant constituents. Other Scorzonera and Podospermum species, potentially containing triterpenic aglycones, were included in the study. An HPLC method for simultaneous determination of triterpene aglycones was therefore developed for analysis of Podospermum and Scorzonera species. n-Hexane extracts of root and aerial parts of S. latifolia, ten other Scorzonera species and two Podospermum species were studied to compare the content of triterpenes. HPLC was used for the qualitative and quantitative analysis of α-amyrin, lupeol, lupeol acetate, taraxasteryl acetate, 3-β-hydroxy-fern-7-en-6-one acetate, urs-12-en-11-one-3-acetyl, 3-β-hydroxy-fern-8-en-7-one acetate, and olean-12-en-11-one-3-acetyl. Limits of detection and quantification were determined for each compound. HPLC fingerprinting of n-hexane extracts of Podospermum and Scorzonera species revealed relatively large amounts of triterpenes in a majority of investigated taxa. Lupeol, lupeol acetate, and taraxasteryl acetate were found in a majority of the species, except S. acuminata. The presence of α-amyrin, 3β-hydroxy-fern-7-en-6-one-acetate, urs-12-en-11-one-3-acetyl, 3β-hydroxy-fern-8-en-7-one-acetate, and olean-12-en-11-one-3-acetyl was detected in varying amounts. The triterpene content could correlate with the analgesic and anti-inflammatory activity of Scorzonera, which was previously observed and Scorzonera species that have been determined to contain triterpenes in large amounts and have not yet been tested for their analgesic activity should be tested for their potential analgesic and anti-inflammatory potential. The presented HPLC method can be used for analysis of triterpene aglycones, for example dedicated to chemosystematic studies of the Scorzonerinae.

scholarly journals Anti-proliferative and anti-inflammatory activities of the sea cucumber Holothuria polii aqueous extract

2018 ◽  
Vol 6 ◽  
pp. 205031211880954 ◽  
Author(s):  
Mike Kareh ◽  
Rana El Nahas ◽  
Lamis Al-Aaraj ◽  
Sara Al-Ghadban ◽  
Nataly Naser Al Deen ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Matrix Metalloproteinase ◽  
Sea Cucumber ◽  
Three Dimensional ◽  
Ethanolic Extract ◽  
Two Dimensional ◽  
Aqueous Fraction ◽  
Anti Inflammatory ◽  
Metalloproteinase 9 ◽  
Holothuria Polii

Objective: Sea cucumbers are considered among the most important functional foods. Following bioassay guided fractionation, we assessed the anti-proliferative and anti-inflammatory activities of Holothuria polii ( H. polii) extracts. Methods: Sea cucumber ethanolic extract and the partially purified aqueous fractions were assessed for their anti-proliferative activities. These latter bioactivities were evaluated in the highly invasive MDA-MB-231 human breast cancer cells in two-dimensional and three-dimensional cultures using trypan blue exclusion assay. The tumor-suppressive effects of sea cucumber ethanolic extract and aqueous fractions were assayed by measuring the trans-well invasion of MDA-MB-231 cells and the expression of some epithelial mesenchymal transition markers using quantitative reverse-transcription polymerase chain reaction and western blot analysis. The anti-inflammatory activity of the aqueous fraction was tested by measuring the secreted levels of interleukin-6, nitric oxide, and matrix metalloproteinase 9 in endotoxin-induced mammary epithelial SCp2 cells and interleukin-1β in phorbol-12-myristate-13-acetate-activated human monocytic THP-1 cells. Results: Sea cucumber ethanolic extract and the aqueous fraction significantly decreased the proliferation of MDA-MB-231 cells by more than 50% at similar and noncytotoxic concentrations and caused an arrest in the S-phase of the cell cycle of treated cells. In contrast, petroleum ether, chloroform, ethyl acetate, and n-butanol organic fractions did not show any significant activity. Furthermore, sea cucumber ethanolic extract and aqueous fraction reduced the proliferation of MDA-MB-231 cells in three-dimensional cultures by more than 60% at noncytotoxic concentrations. In addition, treatment with these concentrations resulted in the loss of stellate outgrowths in favor of spherical aggregates and a 30% decrease in invasive properties. Both sea cucumber ethanolic extract and aqueous decreased the transcription of vimentin and the protein expression levels of vimentin and N-cadherin in three-dimensional cultures. The aqueous fraction decreased the levels of inflammatory markers interleukin-6, nitric oxide, and matrix metalloproteinase 9 in the mouse mammary SCp2 cells, and the level of interleukin-1β produced by phorbol-12-myristate-13-acetate-activated THP-1 human monocytic cells. Conclusion: The data reveal for the first time promising anti-proliferative and anti-inflammatory activities in H. polii water extract in two-dimensional and three-dimensional culture models.

scholarly journals An Ethanolic Extract of Boehmeria caudata Aerial Parts Displays Anti-inflammatory and Anti-tumor Activities

2020 ◽  
Vol 7 (01) ◽  
pp. e17-e25 ◽  
Author(s):  
Paula Pereira de Paiva ◽  
Fabiana Regina Nonato ◽  
Ana Lúcia Tasca Gois Ruiz ◽  
Ilza Maria de Oliveira Sousa ◽  
Rafael Rosolen Teixeira Zafred ◽  
...  
Keyword(s):  
Anticancer Agents ◽  
Ethanolic Extract ◽  
Experimental Models ◽  
New Drugs ◽  
Antiproliferative Effects ◽  
Aerial Parts ◽  
Anti Inflammatory ◽  
Inflammatory Action ◽  
Effective Source

AbstractThe tumor microenvironment presents several therapeutic targets, with inflammation being one of them. In search of new drugs, plants have shown to be an effective source of potent anti-inflammatory and anticancer agents. This study aimed to evaluate the antitumoral and inflammatory activities of Boehmeria caudata aerial parts extract. Bioguided in vitro antiproliferative screening showed that phenanthroquinolizidine obtained from the aerial B. caudata ethanolic extract had a straight relationship with activity. Moreover, the orally administered ethanolic extract reduced Ehrlich solid tumor growth and displayed an anti-inflammatory effect in both evaluated experimental models (carrageenan-induced paw edema and croton oil-induced ear edema). These results suggest that the antitumor activity of the ethanolic extract could be explained by antiproliferative effects associated with anti-inflammatory action.

Anti-inflammatory and analgesic properties of water–ethanolic extract from Pothomorphe umbellata (Piperaceae) aerial parts

2005 ◽  
Vol 99 (2) ◽  
pp. 215-220 ◽  
Author(s):  
Fabio F. Perazzo ◽  
Gustavo H.B. Souza ◽  
Walter Lopes ◽  
Luis G.V. Cardoso ◽  
Jose C.T. Carvalho ◽  
...  
Keyword(s):  
Ethanolic Extract ◽  
Aerial Parts ◽  
Anti Inflammatory

scholarly journals Phytochemical and Pharmacological evaluation of hibiscus hispidissimus griff

2020 ◽  
Vol 11 (SPL4) ◽  
pp. 2063-2067
Author(s):  
Alekhya V ◽  
Ganapaty S ◽  
Deepan T
Keyword(s):  
Radical Scavenging ◽  
Ethanolic Extract ◽  
Phytochemical Analysis ◽  
Zone Of Inhibition ◽  
E Coli ◽  
Aerial Parts ◽  
Paw Oedema ◽  
Anti Inflammatory ◽  
Rat Paw ◽  
Rat Paw Oedema

To assess phytochemical with pharmacological studies of Hibiscus hispidissimus griff belong to family malavaceae. Preliminary phytochemical analysis reveals the presence of steroids, triterpenes, saponins, steroidal saponins and phenols. Evaluation of anti-inflammatory, anti-microbial with antioxidant action were performed on aerial parts of methanolic extract of Hibiscus hispidissimus. Invitro antioxidant activity was performed by 2, 2 -diphenyl- 1- picrylhydrazyl (DPPH) assay, hydroxy radical scavenging method and superoxide radical scavenging activity.The results of invitro antioxidant study reveal that % inhibition of H. hispidissimus  was higher compared to ascorbic acid. Anti-inflammatory studies were performed using carrageenan-induced rat paw oedema animal model, for anti-inflammatory studies, the extracts were compared with standards like indomethacin, and it shows a remarkable zone of inhibition ranging from 58.97 to 71.73 respectively. The anti-bacterial and antifungal activity of plant extracts were studied for the occurrence of inhibition zones. The activity was performed by the cup plate method. Ethanolic extract of H. Hispidissimus  shows significant anti-bacterial effect against S. Aureus, B. Subtilis, P. Vulgaris and E. coli using ciprofloxacin (50µg/ml) as standard.The extracts show remarkable inhibition of zone of inhibition, and results were compared with that of standard drugs against the organism tested. In conclusion, the ethanolic extract of H. hispidissimus  shows  significant antioxidant, anti-inflammatory and anti-bacterial properties.

scholarly journals Anti-inflammatory and antioxidant activities of ethanolic extract of aerial parts of Vernonia patula (Dryand.) Merr.

2013 ◽  
Vol 3 (10) ◽  
pp. 798-805 ◽  
Author(s):  
Arpona Hira ◽  
Shubhra Kanti Dey ◽  
Md. Sariful Islam Howlader ◽  
Arif Ahmed ◽  
Hemayet Hossain ◽  
...  
Keyword(s):  
Antioxidant Activities ◽  
Ethanolic Extract ◽  
Aerial Parts ◽  
Anti Inflammatory

scholarly journals Phytochemical Investigation and Cytotoxic Activity of Rosmarinus officinalis L. Fam. labiateae

2018 ◽  
Vol 9 (12) ◽  
Author(s):  
Abdel-Monem M Ateya ◽  
Maged M. Abou-Hashem ◽  
Gamal A. Dora ◽  
Mohamed M. El-Hoseni
Keyword(s):  
13C Nmr ◽  
Rosmarinus Officinalis ◽  
Hep G2 ◽  
1H And 13C Nmr ◽  
Aerial Parts ◽  
Nmr Data ◽  
Phytochemical Investigation ◽  
13C Nmr Analysis ◽  
Hepatocellular Carcinoma Cell Line ◽  
Rosmarinus Officinalis L

A new active rolyeanone-type diterpenoid p-quinone (Moquinone) against hepatocellular carcinoma cell line Hep G2 together with four known compounds were isolated from the aerial parts of Rosmarinus officinalis L. The structure of the new compound was determined by extensive spectroscopic analysis including IR, EIMS, 1D and 2D 400MHz NMR data (1H, 13C NMR, DEPT 135 and 1H1H COSY), whereas the structure of the known compounds were identified by comparison of their IR, MS, 1H and 13C NMR analysis with those reported in the literature data.

scholarly journals A New Anti-inflammatory Phenolic Monosaccharide from the Australian Native Rainforest Plant Elaeocarpus Eumundi

2018 ◽  
Vol 13 (6) ◽  
pp. 1934578X1801300
Author(s):  
Ahilya Singh ◽  
Gerald Münch ◽  
Paul Reddell ◽  
Michael Radzieta ◽  
Slade O. Jensen ◽  
...  
Keyword(s):  
Chemical Analysis ◽  
Ethanolic Extract ◽  
2D Nmr ◽  
Inflammatory Activity ◽  
Raw 264.7 ◽  
Spectroscopic Methods ◽  
Nmr Data ◽  
Raw 264.7 Macrophage Cells ◽  
Ifn Γ ◽  
Anti Inflammatory

Chemical analysis of the ethanolic extract of the Australian rainforest plant Elaeocarpus eumundi yielded a new phenolic monosaccharide (1) and the known dihydropieceid (2). The structures of both compounds were elucidated based on the spectroscopic methods including UV, HR-ESIMS and 1D, 2D NMR data. Compounds 1 and 2 exhibited good anti-inflammatory activity in LPS and IFN-γ activated RAW 264.7 macrophage cells.

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