Analysis Of İnterleukin-1 Receptor Antagonist Variable Number Tandem Repeat Variant in Recurrent Aphthous Stomatitis

2020 ◽  
Vol 20 ◽  
Author(s):  
Akin Tekcan ◽  
Serbulent Yigit ◽  
Ayse F. Nursal ◽  
Mehmet K. Tumer ◽  
Kaan Yerliyurt ◽  
...  
Keyword(s):  
Receptor Antagonist ◽  
Tandem Repeat ◽  
Interleukin 1 ◽  
Variable Number Tandem Repeat ◽  
Variable Number ◽  
Recurrent Aphthous Stomatitis ◽  
Aphthous Stomatitis ◽  
Genotype Distribution ◽  
Healthy Controls ◽  
Interleukin 1 Receptor Antagonist

Background/Aims: Recurrent aphthous stomatitis (RAS) is one of common oral inflammatory diseases. As immunological and genetic factors have been held in the pathogenesis of RAS, the objective of this study was to determine whether the interleukin-1 receptor antagonist (IL-1Ra) gene variable number tandem repeat (VNTR) variant is a risk factor for the development of RAS in Turkish patients and to define its contribution to the increased risk. Methods: The IL-1Ra VNTR variant was evaluated in 169 RAS patients and 171 healthy controls by the polymerase chain reaction (PCR) method. Results: No statistically significant difference was found in the genotype distribution and allelic frequencies of IL-1Ra VNTR variant between RAS patients and healthy controls. Conclusion: Lack of association between IL-1Ra VNTR variant and RAS could indicate that IL-1Ra has no significant role in pathophysiology of RAS. However, it still appears to be very worthwhile to continue to search for cytokine gene variants in order to predict the development of such disease.

scholarly journals IL-1RN VNTR Polymorphism in Adult Dermatomyositis and Systemic Lupus Erythematosus

2014 ◽  
Vol 2014 ◽  
pp. 1-5 ◽  
Author(s):  
Zornitsa Kamenarska ◽  
Gyulnas Dzhebir ◽  
Maria Hristova ◽  
Alexey Savov ◽  
Anton Vinkov ◽  
...  
Keyword(s):  
Systemic Lupus Erythematosus ◽  
Receptor Antagonist ◽  
Lupus Erythematosus ◽  
Interleukin 1 ◽  
Variable Number Tandem Repeat ◽  
Variable Number ◽  
Systemic Lupus ◽  
Vntr Polymorphism ◽  
Interleukin 1 Receptor Antagonist ◽  
Control Study

Polymorphisms in the cytokine genes and their natural antagonists are thought to influence the predisposition to dermatomyositis (DM) and systemic lupus erythematosus (SLE). A variable number tandem repeat (VNTR) polymorphism of 86 bp in intron 2 of the interleukin-1 receptor antagonist (IL-1RN) gene leads to the existence of five different alleles which cause differences in the production of both IL-1RA (interleukin-1 receptor antagonist) and IL-1β. The aim of this case-control study was to investigate the association between the IL-1RN VNTR polymorphism and the susceptibility to DM and SLE in Bulgarian patients. Altogether 91 patients, 55 with SLE and 36 with DM, as well as 112 unrelated healthy controls, were included in this study. Only three alleles were identified in both patients and controls ((1) four repeats, (2) two repeats, and (3) five repeats). The IL-1RN*2 allele (P=0.02, OR 2.5, and 95% CI 1.2–5.4) and the 1/2+2/2 genotypes were found prevalent among the SLE patients (P=0.05, OR 2.6, and 95% CI 1–6.3). No association was found between this polymorphism and the ACR criteria for SLE as well as with the susceptibility to DM. Our results indicate that the IL-1RN VNTR polymorphism might play a role in the susceptibility of SLE but not DM.

scholarly journals Lack of association of Graves' disease with the A2 allele of the interleukin-1 receptor antagonist gene in a white European population

1998 ◽  
pp. 686-690 ◽  
Author(s):  
T Muhlberg ◽  
M Kirchberger ◽  
C Spitzweg ◽  
F Herrmann ◽  
HJ Heberling ◽  
...  
Keyword(s):  
Receptor Antagonist ◽  
Interleukin 1 ◽  
Variable Number Tandem Repeat ◽  
Variable Number ◽  
European Population ◽  
Allele Frequencies ◽  
Control Group ◽  
Graves Disease ◽  
Interleukin 1 Receptor Antagonist ◽  
Graves Ophthalmopathy

OBJECTIVE: To assess whether the A2-type IL-1RA polymorphism is associated with Graves' disease and Graves' ophthalmopathy. Several reports have described a genetic association between the A2 allele of the interleukin-1 receptor antagonist (IL-1RA) gene and certain inflammatory and autoimmune diseases, suggesting that certain loci within the IL-1-related genes may modulate the autoimmune inflammatory response. Recently, we demonstrated marked differences in the expression and regulation of IL-1RA gene and protein between orbital fibroblasts derived from patients with active Graves' ophthalmopathy and healthy individuals. DESIGN: A total of 144 white European patients with Graves' disease were genotyped to compare their IL-1RA A2 allele frequency with that of 174 healthy controls. METHODS: The polymerase chain reaction was used to amplify the pentallelic variable-number tandem-repeat locus in intron 2 of the IL-1RA gene. RESULTS: We found no significant differences in IL-1RA A2 allele frequencies (0.20 and 0.26 respectively) and IL-1RA A2 carriage rates (31% and 40% respectively) between patients with Graves' disease and the control group. Moreover, presence or absence of Graves' ophthalmopathy in patients with Graves' disease was not related to significant differences in IL-1RA A2 allele frequencies and IL-1RA A2 carriage rates. CONCLUSIONS: Our data do not support an association between the IL-1RA A2 allele and Graves' disease or Graves' ophthalmopathy in our study population. Thus the A2-type IL-1RA gene polymorphism does not appear to indicate an increased susceptibility to develop Graves' disease and Graves' ophthalmopathy. Mechanisms unrelated to the IL-1RA A2 allele may be responsible for altered IL-1RA production within the orbital tissues in Graves' ophthalmopathy.

Sign in / Sign up

Export Citation Format

Share Document