Antibiotics and Micronutritional Blend to Enhance Fertility Potential in Male Having Abnormal Semen Parameters

Because the assessment of sperm DNA fragmentation (SDF) plays a key role in male fertility, our study was designed to find the relationships between SDF and standard semen parameters. The receiver operating characteristic (ROC) curve showed that 18% SDF is a prognostic parameter for discriminating between men with normal and abnormal standard semen parameters (n = 667). Men with > 18% SDF had significantly lower quality semen, a higher prevalence of abnormal semen characteristics, and a higher odds ratio for abnormal semen parameters compared to men with ≤ 18% SDF. An ROC analysis provided predictive values for age and semen parameters to distinguish between men with SDF > 18% and men with ≤ 18% SDF. SDF was positively correlated with male age and teratozoospermia index but negatively with sperm concentration, total number of spermatozoa, sperm morphology, progressive motility, and vitality. Our study shows that 18% SDF has a predictive value for distinguishing between men with normal and abnormal semen characteristics. Men with >18% SDF have a higher risk for abnormal semen parameters, while age and obtained semen parameters have a predictive value for SDF. There is a relationship between SDF and conventional sperm characteristics, and thus, SDF can be incorporated into male fertility assessment.

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Investigation the effects of varicocelectomy on sex hormones levels and semen parameters

World Journal of Biology Pharmacy and Health Sciences ◽

10.30574/wjbphs.2021.6.2.0040 ◽

2021 ◽

Vol 6 (2) ◽

pp. 07-013

Author(s):

Fatemeh Soheili ◽

Mohamad Falahati

Keyword(s):

Surgical Intervention ◽

Sperm Count ◽

Cross Sectional Study ◽

Semen Parameters ◽

Sexual Disorders ◽

Sexual Hormones ◽

Cross Sectional ◽

Fertility Potential ◽

Positive Effect

Background: Varicocelectomy is a type of surgery for varicocele treatment. This may recover fertility properties in males through improvement in semen parameters as well as sexual hormones. In this study, the role of varicocelectomy with focus on semen properties and sexual hormones have been assessed. Methods: In this cross-sectional study, before- after data from fifteen patients with different varicocele grade, regarding to varicocelectomy have been compared. Result: After varicocelectomy, a significant improvement in some semen parameters such as motility and concentration has been showed. However, among sexual hormones, only testosterone has been increased after surgical intervention (p=0.007). Conclusion: Based on our knowledge, varicocele treatment may almost improve fertility potential in males significantly. Accordingly, it seems that varicocelectomy has positive effect on parameters such as testosterone and serum FSH levels as well as sperm count and motility. Therefore, it could be concluded that varicocelectomy is an effective approach to improve male fertility and sexual disorders.

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Evaluation of the influence of subinguinal varicocelectomy procedure on seminal parameters, reproductive hormones and testosterone/estradiol ratio

Archivio Italiano di Urologia e Andrologia ◽

10.4081/aiua.2020.2.158 ◽

2020 ◽

Vol 92 (2) ◽

Author(s):

Ünal Öztekin ◽

Mehmet Caniklioglu ◽

Sercan Sarı ◽

Volkan Selmi ◽

Abdullah Gürel ◽

...

Keyword(s):

Male Infertility ◽

Serum Testosterone ◽

Reproductive Hormones ◽

Semen Parameters ◽

Testosterone Levels ◽

Seminal Parameters ◽

Semen Volume ◽

The Mean ◽

Fertility Potential ◽

Grade 3

Objective: Varicocele is the most commonly surgically curable cause of male infertility. However, the mechanisms related to the effect of reducing fertility potential have not been clearly identified. The aim of this study was to investigate the effects of varicocelectomy on semen parameters, reproductive hormones and testosterone / estradiol ratio. Matherial and methods: Fifty seven patients outcomes were evaluated before and 6 months after subinguinal microsurgical varicocelectomy. Semen parameters, reproductice hormones and testosteron/estradiol ratio results of patients were compared retrospectively. Results: The mean age was 26.8 years. Fifty four (94.7%) patients had grade 3 and 3 (5.3%) patients had grade 2 varicocele. There was a significant increase in semen parameters except semen volume. There was a statistically significant increase in serum testosterone levels, but not on testosterone/ estradiol ratio. Conclusions: According to our results, microsurgical subinguinal varicocelectomy can be recommended for both improving semen parameters and hormonal recovery.

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Paper-Based Quantification of Male Fertility Potential

Clinical Chemistry ◽

10.1373/clinchem.2015.250282 ◽

2016 ◽

Vol 62 (3) ◽

pp. 458-465 ◽

Cited By ~ 34

Author(s):

Reza Nosrati ◽

Max M Gong ◽

Maria C San Gabriel ◽

Claudio E Pedraza ◽

Armand Zini ◽

...

Keyword(s):

Sperm Motility ◽

Male Fertility ◽

Semen Analysis ◽

Sperm Concentration ◽

Absolute Humidity ◽

Semen Parameters ◽

Motile Sperm ◽

Clinical Practices ◽

Male Factor ◽

Fertility Potential

Abstract BACKGROUND More than 70 million couples worldwide are affected by infertility, with male-factor infertility accounting for about half of the cases. Semen analysis is critical for determining male fertility potential, but conventional testing is costly and complex. Here, we demonstrate a paper-based microfluidic approach to quantify male fertility potential, simultaneously measuring 3 critical semen parameters in 10 min: live and motile sperm concentrations and sperm motility. METHODS The device measures the colorimetric change of yellow tetrazolium dye to purple formazan by the diaphorase flavoprotein enzyme present in metabolically active human sperm to quantify live and motile sperm concentration. Sperm motility was determined as the ratio of motile to live sperm. We assessed the performance of the device by use of clinical semen samples, in parallel with standard clinical approaches. RESULTS Detection limits of 8.46 and 15.18 million/mL were achieved for live and motile sperm concentrations, respectively. The live and motile sperm concentrations and motility values from our device correlated with those of the standard clinical approaches (R2 ≥ 0.84). In all cases, our device provided 100% agreement in terms of clinical outcome. The device was also robust and could tolerate conditions of high absolute humidity (22.8 g/m3) up to 16 weeks when packaged with desiccant. CONCLUSIONS Our device outperforms existing commercial paper-based assays by quantitatively measuring live and motile sperm concentrations and motility, in only 10 min. This approach is applicable to current clinical practices as well as self-diagnostic applications.

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Administration of antioxidants in the infertile male: When may it have a detrimental effect?

Current Pharmaceutical Design ◽

10.2174/1381612827666210204160248 ◽

2021 ◽

Vol 27 ◽

Author(s):

Fotios Dimitriadis ◽

Evangelos N. Symeonidis ◽

Panagiota Tsounapi ◽

Aris Kaltsas ◽

Georgios Hatzichristodoulou ◽

...

Keyword(s):

Male Infertility ◽

Detrimental Effect ◽

Semen Parameters ◽

Oxygen Species ◽

Over The Counter ◽

Risks And Benefits ◽

Promising Solution ◽

Fertility Potential ◽

Nutritional Compounds ◽

Shed Light

Background & Objective: Significant advances in the field of male infertility have been witnessed during the last years. Given the well-known detrimental effects of reactive oxygen species (ROS), the administration of antioxidants has emerged as a promising solution for oxidative stress (OS)-induced male infertility. Nevertheless, this perception seems largely oversimplified, and the existing literature fails to recognize a notable superiority of the excessive use of these widely available nutritional compounds. Taking into consideration that several trials have shed light on the so-called “antioxidant paradox” phenomenon, we recognize that over-the-counter consumption of such supplements might be harmful. Method: The relevant studies indexed in PubMed, Google Scholar and Scopus databases, published until September 2019 were identified and reported. Conclusion: In this setting, we acknowledge that there is an eminent need for more elaborate studies in the future which will efficiently elucidate the risks and benefits of antioxidants on semen parameters and their impact on fertility potential.

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262 SPERM CHROMATIN STRUCTURE, OXIDATIVE STRESS AND BASIC SEMEN PARAMETERS OF MEN FROM SUBFERTILE COUPLES

Reproduction Fertility and Development ◽

10.1071/rdv17n2ab262 ◽

2005 ◽

Vol 17 (2) ◽

pp. 281

Author(s):

M. Bochenek ◽

P. Gogol ◽

J. Janeczko

Keyword(s):

Chromatin Structure ◽

Sperm Concentration ◽

World Health ◽

Semen Parameters ◽

Sperm Chromatin ◽

Sperm Chromatin Structure Assay ◽

Progressive Motility ◽

Fertility Potential ◽

Health Organization ◽

High Level

It is known that the mammalian sperm chromatin structure plays an important role in male fertility. In opposition to many other areas of biological research, the human sperm chromatin can be considered as a model for animal fertility investigations. This is due to the great number of males with high levels of chromatin abnormalities and the ease of tracking their fertility potential. The aim of the study was to find a relationship between sperm chromatin structure, level of reactive oxygen species (ROS) and the basic semen parameters: sperm concentration and motility. The semen from a total of 391 men from subfertile couples 22–51 years old was used. The sperm chromatin abnormalities were examined flow cytometrically according to the SCSA method (sperm chromatin structure assay; Evenson D.P. Methods In Cell Biology, vol. 33, 1990) and ROS level was examined by luminometry (Kolletis et al. 1999 Fertil. Steril.). Sperm concentration and motility were checked microscopically. Sperm concentration of the examined ejaculates ranged from 0.05 × 106/mL to 627.5 × 106/mL and progressive motility ranged from 0% to 70%. More than 30% of spermatozoa with abnormal chromatin (level considered as the infertility threshold) was found in 70 (17.9%) patients; 15–30% of spermatozoa with abnormal chromatin (level of decreased fertility potential) was found in 154 (39.4%) patients; and in 167 (42.7%) patients the number of abnormal spermatozoa did not exceed 15% (level of normal fertility potential; Evenson et al. 1999 Hum. Reprod.; Zini et al. 2001 Fertil. Steril.). High significant correlations were found between chromatin abnormality and: patients' age (0.1008, P = 0.017), sperm concentration (−0.2735, P < 0.001), progressive motility (−0.4365, P < 0.001), and ROS level (0.2709, P < 0.001). However in patients with normal sperm concentration (>20 × 106/mL, according to the World Health Organization), as many as 11.5% had a high level of chromatin abnormality (>30% of abnormal chromatin) and 29.7% of moderate chromatin abnormality (15–30% abnormal chromatin). Similarly, in patients with normal progressive sperm motility (>50%, according to the World Health Organization) 1.7% had a high level of chromatin abnormality (>30% of abnormal chromatin), and 33.9% had a moderate level of chromatin abnormality (15–30% abnormal chromatin). Contrary to the findings of many earlier investigations, a strong relationship between sperm chromatin damages and basic semen parameters was observed in this work. The sperm chromatin structure assay should be included in standard semen examination to avoid expensive and time consuming in vitro procedures for spermatozoa with damaged DNA.

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The influence of Robertsonian translocations on semen parameters

Nauchno-prakticheskii zhurnal «Medicinskaia genetika» ◽

10.25557/2073-7998.2020.03.87-88 ◽

2020 ◽

pp. 87-88

Author(s):

М.В. Андреева ◽

М.И. Штаут ◽

Т.М. Сорокина ◽

Л.Ф. Курило ◽

В.Б. Черных

Keyword(s):

Male Infertility ◽

Semen Parameters ◽

Meiotic Arrest ◽

Robertsonian Translocations ◽

Prophase I ◽

Infertile Men ◽

Fertility Problems ◽

Spermatogenesis Impairment

Обследованы 19 мужчин с нарушением фертильности, носителей транслокаций rob(13;14) и rob(13;15). Показано, что нарушение репродуктивной функции обусловлено блоком сперматогенеза в профазе I мейоза, приводящего к азооспермии или олигоастенотератозооспермии и мужскому бесплодию. We examined 19 infertile men, carriers of translocations rob (13;14) and rob (13;15). We assume that fertility problems are resulted from spermatogenesis impairment because of meiotic arrest at prophase I stages, that leads to azoospermia or oligoastenoteratozoospermia and male infertility.

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