Deep Row Incorporation of Biosolids to Grow Hybrid Poplar Trees on Gravel Spoils in Southern Maryland: Impacts on Water Quality, Tree Growth & Survival, Profitability, and the Environment

2006 ◽  
Vol 2006 (2) ◽  
pp. 332-354
Author(s):  
Kays, J. ◽  
Buswell, C. ◽  
Felton, G.K. ◽  
E. Flamino
2001 ◽  
Vol 3 (1) ◽  
pp. 87-104 ◽  
Author(s):  
A. Ferro ◽  
J. Chard ◽  
R. Kjelgren ◽  
B. Chard ◽  
D. Turner ◽  
...  
Keyword(s):  

2007 ◽  
Vol 85 (11) ◽  
pp. 1071-1081 ◽  
Author(s):  
Edward J. Harrison ◽  
Michael Bush ◽  
Jonathan M. Plett ◽  
Daniel P. McPhee ◽  
Robin Vitez ◽  
...  

We have produced the largest population of activation-tagged poplar trees to date, approximately 1800 independent lines, and report on phenotypes of interest that have been identified in tissue culture and greenhouse conditions. Activation tagging is an insertional mutagenesis technique that results in the dominant upregulation of an endogenous gene. A large-scale Agrobacterium -mediated transformation protocol was used to transform the pSKI074 activation-tagging vector into Populus tremula × Populus alba hybrid poplar. We have screened the first 1000 lines for developmental abnormalities and have a visible mutant frequency of 2.4%, with alterations in leaf and stem structure as well as overall stature. Most of the phenotypes represent new phenotypes that have not previously been identified in poplar and, in some cases, not in any other plant either. Molecular analysis of the T-DNA inserts of a subpopulation of mutant lines reveal both single and double T-DNA inserts with double inserts more common in lines with visible phenotypes. The broad range of developmental mutants identified in this pilot screen of the population reveals that it will be a valuable resource for gene discovery in poplar. The full value of this population will only be realized as we screen these lines for a wide range of phenotypes.


2003 ◽  
Author(s):  
J.P. Chastain ◽  
E.D. Dickens ◽  
W.D. Lucas ◽  
J.J. Camberato ◽  
K.P. Moore

2008 ◽  
Vol 148 (3) ◽  
pp. 1229-1237 ◽  
Author(s):  
Heather D. Coleman ◽  
A. Lacey Samuels ◽  
Robert D. Guy ◽  
Shawn D. Mansfield

2004 ◽  
Vol 6 (2) ◽  
pp. 157-167 ◽  
Author(s):  
Xingmao Ma ◽  
Andrew R. Richter ◽  
Sarah Albers ◽  
Joel G. Burken
Keyword(s):  

2021 ◽  
Author(s):  
Minglei Yi ◽  
Heyu Yang ◽  
Shaohui Yang ◽  
Jiehua Wang

Plant branching is usually prevented by an actively proliferating apex. In poplars, one GRAS family member, SHORT-ROOT2 (PtSHR2), was preferentially expressed in axillary buds (AXBs) and was inducible during bud maturation and activation. Overexpression of PtSHR2 (PtSHR2OE) in hybrid poplar impaired the apical dominance and simultaneously promoted the outgrowth of axillary branches below the maturation point (BMP), accompanied by regulated expression of genes critical for axillary meristem initiation and bud formation. Following a detained phenotypic characterization, we compared the IAA and trans-zeatin levels in apical shoots and AXBs of wild-type and PtSHR2OE trees, together with gene expression analyses and defoliation, decapitation, and hormone reapplication assays. PtSHR2OE AXBs contained a significantly lower ratio of auxin to cytokinin than wild-type AXBs, particularly in those below the BMP. Decapitation induced a faster bud burst in PtSHR2OE trees than in wild-type plants, and it could be strongly inhibited by exogenously applied auxin and cytokinin biosynthesis inhibitor, but only partially inhibited by N-1-naphthylphthalamic acid (NPA). An impaired basipetal auxin transport, rather than an insufficient auxin biosynthesis or auxin insensitivity, disturbed the local hormonal homeostasis in PtSHR2OE AXBs, which in turn enhanced the axillary bud initiation and promoted the bud release.


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