Importance of syntrophic long-chain fatty acid-degrading bacteria during anaerobic co-digestion of fats, oils, and grease with municipal wastewater sludge

2015 ◽  
Vol 2015 (11) ◽  
pp. 6569-6575
Author(s):  
Ryan M Ziels ◽  
Anna Karlsson ◽  
Jörgen Ejlertsson ◽  
Sepehr Yekta ◽  
Annika Bjorn ◽  
...  
Keyword(s):  
Fatty Acid ◽  
Municipal Wastewater ◽  
Chain Fatty Acid ◽  
Wastewater Sludge ◽  
Long Chain Fatty Acid ◽  
Degrading Bacteria ◽  
Long Chain ◽  
Oils And Grease

scholarly journals Diversity of Anaerobic Microorganisms Involved in Long-Chain Fatty Acid Degradation in Methanogenic Sludges as Revealed by RNA-Based Stable Isotope Probing

2007 ◽  
Vol 73 (13) ◽  
pp. 4119-4127 ◽  
Author(s):  
Masashi Hatamoto ◽  
Hiroyuki Imachi ◽  
Yuto Yashiro ◽  
Akiyoshi Ohashi ◽  
Hideki Harada
Keyword(s):  
Fatty Acid ◽  
Stable Isotope ◽  
Major Fatty Acid ◽  
Stable Isotope Probing ◽  
Chain Fatty Acid ◽  
Long Chain Fatty Acid ◽  
Bacterial Populations ◽  
Anaerobic Microorganisms ◽  
Degrading Bacteria ◽  
Long Chain

ABSTRACT Long-chain fatty acid (LCFA) degradation is a key step in methanogenic treatment of wastes/wastewaters containing high concentrations of lipids. However, despite the importance of LCFA-degrading bacteria, their natural diversity is little explored due to the limited availability of isolate information and the lack of appropriate molecular markers. We therefore investigated these microbes by using RNA-based stable isotope probing. We incubated four methanogenic sludges (mesophilic sludges MP and MBF and thermophilic sludges TP and JET) with 13C-labeled palmitate (1 mM) as a substrate. After 8 to 19 days of incubation, we could detect 13C-labeled bacterial rRNA. A density-resolved terminal restriction fragment length polymorphism fingerprinting analysis showed distinct bacterial populations in 13C-labeled and unlabeled rRNA fractions. The bacterial populations in the 13C-labeled rRNA fractions were identified by cloning and sequencing of reverse-transcribed 16S rRNA. Diverse phylogenetic bacterial sequences were retrieved, including those of members of the family Syntrophaceae, clone cluster MST belonging to the class Deltaproteobacteria, Clostridium clusters III and IV, phylum Bacteroidetes, phylum Spirochaetes, and family Syntrophomonadaceae. Although Syntrophomonadaceae species are considered to be the major fatty acid-degrading syntrophic microorganisms under methanogenic conditions, they were detected in only two of the clone libraries. These results suggest that phylogenetically diverse bacterial groups were active in situ in the degradation of LCFA under methanogenic conditions.

scholarly journals Long-chain fatty acid-induced changes in gene expression in neonatal cardiac myocytes

2000 ◽  
Vol 41 (1) ◽  
pp. 41-47 ◽  
Author(s):  
Karin A. J.M. van der Lee ◽  
Michaël M. Vork ◽  
Johan E. De Vries ◽  
Peter H.M. Willemsen ◽  
Jan F.C. Glatz ◽  
...  
Keyword(s):  
Gene Expression ◽  
Fatty Acid ◽  
Cardiac Myocytes ◽  
Chain Fatty Acid ◽  
Long Chain Fatty Acid ◽  
Induced Changes ◽  
Long Chain

scholarly journals 588 Distinct Roles of the Long-Chain Fatty Acid Receptor GPR120 in Different Cell Types of the Intestinal Epithelium

2014 ◽  
Vol 146 (5) ◽  
pp. S-110-S-111
Author(s):  
Arivarasu Natarajan Anbazhagan ◽  
Shubha Priyamvada ◽  
Tarunmeet Gujral ◽  
Waddah A. Alrefai ◽  
Pradeep K. Dudeja ◽  
...  
Keyword(s):  
Fatty Acid ◽  
Intestinal Epithelium ◽  
Cell Types ◽  
Chain Fatty Acid ◽  
Long Chain Fatty Acid ◽  
Acid Receptor ◽  
Different Cell Types ◽  
Long Chain ◽  
Fatty Acid Receptor
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