scholarly journals Manganese Superoxide Dismutase Activity Assay in the Yeast Saccharomyces cerevisiae

BIO-PROTOCOL ◽  
2020 ◽  
Vol 10 (5) ◽  
Author(s):  
Louise Thines ◽  
Pierre Morsomme
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Superoxide Dismutase ◽  
Manganese Superoxide Dismutase ◽  
Superoxide Dismutase Activity ◽  
Activity Assay ◽  
Yeast Saccharomyces Cerevisiae ◽  
Manganese Superoxide

Reduced mitochondrial manganese-superoxide dismutase activity exacerbates glutamate toxicity in cultured mouse cortical neurons

1998 ◽  
Vol 814 (1-2) ◽  
pp. 164-170 ◽  
Author(s):  
Yibing Li ◽  
Jean-Christophe Copin ◽  
Liza F Reola ◽  
Bernard Calagui ◽  
Glenn T Gobbel ◽  
...  
Keyword(s):  
Superoxide Dismutase ◽  
Cortical Neurons ◽  
Manganese Superoxide Dismutase ◽  
Superoxide Dismutase Activity ◽  
Glutamate Toxicity ◽  
Manganese Superoxide

The effect of tumor necrosis factor-α on human malignant glial cells

1992 ◽  
Vol 76 (4) ◽  
pp. 652-659 ◽  
Author(s):  
Rolando F. Del Maestro ◽  
Monica Lopez-Torres ◽  
Warren B. McDonald ◽  
Eric C. Stroude ◽  
Indrasen S. Vaithilingam
Keyword(s):  
Superoxide Dismutase ◽  
Tumor Necrosis Factor ◽  
Cell Line ◽  
Tumor Necrosis ◽  
Manganese Superoxide Dismutase ◽  
Tumor Necrosis Factor Α ◽  
Superoxide Dismutase Activity ◽  
Manganese Superoxide ◽  
Factor Α ◽  
Necrosis Factor

✓ The influence of human recombinant tumor necrosis factor-α has been assessed on a cell line (U-251) derived from a human malignant glial tumor. The results of this study demonstrate that tumor necrosis factor-α at doses of 50 and 100 ng/ml: 1) did not have cytotoxic or cytostatic effects on the U-251 cell line; 2) significantly increased the intracellular activity of manganese superoxide dismutase but had no effect on copper and zinc superoxide dismutase, catalase, or glutathione peroxidase activity; and 3) did not significantly alter the intracellular or extracellular general protease and collagenase type IV activity of these cells. The resistance of the U-251 cell line to tumor necrosis factor-α cytotoxicity may be related in part to the high intrinsic manganese superoxide dismutase activity present in this cell line combined with the ability of this cell line to induce substantial amounts of protective manganese superoxide dismutase activity in response to tumor necrosis factor-α.

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