scholarly journals PENGEMBANGAN PROSES PEMBUATAN BIOETANOL GENERASI II DARI LIMBAH TANDAN KOSONG KELAPA SAWIT

2021 ◽  
Vol 9 (4) ◽  
pp. 233-240
Author(s):  
Hanifah Khairiah ◽  
◽  
Muhammad Ridwan ◽  
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Aspergillus Oryzae

Tandan kosong kelapa sawit (TKKS) yang merupakan sumber gula mengandung selulosa yang tinggi (75-80%), sehingga memiliki potensi besar untuk dijadikan bioetanol. Tujuan penelitian adalah menggunakan kembali tandan kosong kelapa sawit sebagai bahan utama dalam pembuatan bioetanol generasi II dengan melakukan pengembangan proses. Proses pertama yaitu pretreatment dengan menambahkan NaOH dan H2SO4 2%, dilanjutkan proses fermentasi menggunakan perbandingan Saccharomyces cerevisiae and Aspergillus oryzae dengan perbandingan 5,10,15 dan 20% selama 7 hari. Terakhir adalah proses destilasi pada suhu 79 oC. Berdasarkan hasil penelitian, larutan NaOH lebih banyak menurunkan kadar lignin dan hemiselulosa sebesar 12.22% dan 45.17%, sedangkan selulosa mengalami kenaikan sebesar 71.34%. pada proses fermentasi, maka didapatkan variasi konsentrasi dan waktu optimum proses fermentasi pada penelitian ini adalah penambahan konsentrasi Saccharomyces cerevisiae 5%, dengan volume bioetanol yang dihasilkan sebesar 14.4 ml, densitas 0.8757 g/ml dan kadar glukosa yang tertinggal sebanyak 8.48%.

scholarly journals High-Level Secretory Production of Phospholipase A1by Saccharomyces cerevisiae and Aspergillus oryzae

2001 ◽  
Vol 65 (1) ◽  
pp. 94-101 ◽  
Author(s):  
Yoichiro SHIBA ◽  
Chiho ONO ◽  
Fumio FUKUI ◽  
Ichiro WATANABE ◽  
Nobufusa SERIZAWA ◽  
...  
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Aspergillus Oryzae ◽  
Secretory Production ◽  
High Level

Alcoholic chestnut fermentation in mixed culture. Compatibility criteria between Aspergillus oryzae and Saccharomyces cerevisiae strains

2008 ◽  
Vol 99 (15) ◽  
pp. 7255-7263 ◽  
Author(s):  
Miguel Anxo Murado ◽  
Lorenzo Pastrana ◽  
José Antonio Vázquez ◽  
Jesús Mirón ◽  
María Pilar González
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Mixed Culture ◽  
Aspergillus Oryzae

scholarly journals Characterization of a Maltase from an Early-Diverged Non-Conventional Yeast Blastobotrys adeninivorans

2019 ◽  
Vol 21 (1) ◽  
pp. 297 ◽  
Author(s):  
Triinu Visnapuu ◽  
Aivar Meldre ◽  
Kristina Põšnograjeva ◽  
Katrin Viigand ◽  
Karin Ernits ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Saccharomyces Cerevisiae ◽  
Aspergillus Oryzae ◽  
Hydrolysis Product ◽  
Hydrolytic Activity ◽  
Glycoside Hydrolases ◽  
Arxula Adeninivorans ◽  
Sequence Identity ◽  
Diabetes Drug

Genome of an early-diverged yeast Blastobotrys (Arxula) adeninivorans (Ba) encodes 88 glycoside hydrolases (GHs) including two α-glucosidases of GH13 family. One of those, the rna_ARAD1D20130g-encoded protein (BaAG2; 581 aa) was overexpressed in Escherichia coli, purified and characterized. We showed that maltose, other maltose-like substrates (maltulose, turanose, maltotriose, melezitose, malto-oligosaccharides of DP 4‒7) and sucrose were hydrolyzed by BaAG2, whereas isomaltose and isomaltose-like substrates (palatinose, α-methylglucoside) were not, confirming that BaAG2 is a maltase. BaAG2 was competitively inhibited by a diabetes drug acarbose (Ki = 0.8 µM) and Tris (Ki = 70.5 µM). BaAG2 was competitively inhibited also by isomaltose-like sugars and a hydrolysis product—glucose. At high maltose concentrations, BaAG2 exhibited transglycosylating ability producing potentially prebiotic di- and trisaccharides. Atypically for yeast maltases, a low but clearly recordable exo-hydrolytic activity on amylose, amylopectin and glycogen was detected. Saccharomyces cerevisiae maltase MAL62, studied for comparison, had only minimal ability to hydrolyze these polymers, and its transglycosylating activity was about three times lower compared to BaAG2. Sequence identity of BaAG2 with other maltases was only moderate being the highest (51%) with the maltase MalT of Aspergillus oryzae.

scholarly journals The effect of Saccharomyces cerevisiae and Aspergillus oryzae on the digestion of the cell wall fraction of a mixed diet in defaunated and refaunated sheep rumen

1998 ◽  
Vol 38 (4) ◽  
pp. 401-416 ◽  
Author(s):  
Jean-Pierre Jouany ◽  
Frédérique Mathieu ◽  
Jean Senaud ◽  
Jacques Bohatier ◽  
Gérard Bertin ◽  
...  
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Cell Wall ◽  
Aspergillus Oryzae ◽  
Mixed Diet ◽  
Cell Wall Fraction ◽  
Sheep Rumen

scholarly journals The effect of Saccharomyces cerevisiae and Aspergillus oryzae on fermentations in the rumen of faunated and defaunated sheep; protozoal and probiotic interactions

1996 ◽  
Vol 36 (3) ◽  
pp. 271-287 ◽  
Author(s):  
F. Mathieu ◽  
JP Jouany ◽  
J. Sénaud ◽  
J. Bohatier ◽  
G. Bertin ◽  
...  
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Aspergillus Oryzae

scholarly journals Evaluation of the Combined Use of Saccharomyces Cerevisiae and Aspergillus Oryzae with Phytase Fermentation Products on Growth, Inflammatory, and Intestinal Morphology in Broilers

Animals ◽  
2019 ◽  
Vol 9 (12) ◽  
pp. 1051 ◽  
Author(s):  
Wen Yang. Chuang ◽  
Wei Chih. Lin ◽  
Yun Chen. Hsieh ◽  
Chung Ming. Huang ◽  
Shen Chang. Chang ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Saccharomyces Cerevisiae ◽  
Mrna Expression ◽  
Aspergillus Oryzae ◽  
Mononuclear Cells ◽  
Intestinal Morphology ◽  
Soy Sauce ◽  
Feed Additive ◽  
Fermentation Products ◽  
Fermented Products

Saccharomyces cerevisiae and Aspergillus oryzae are both ancient probiotic species traditionally used as microbes for brewing beer and soy sauce, respectively. This study investigated the effect of adding these two probiotics with phytase fermentation products to the broilers diet. Fermented products possess protease and cellulase, and the activities were 777.1 and 189.5 U/g dry matter (DM) on S. cerevisiae fermented products (SCFP) and 190 and 213.4 U/g DM on A. oryzae fermented products (AOFP), respectively. Liposaccharides stimulated PBMCs to produce nitric oxide to 120 μmol. Both SCFP and AOFP reduced lipopolysaccharides stimulated peripheral blood mononuclear cells (PBMCs) nitric oxide release to 40 and 60 μmol, respectively. Nevertheless, in an MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay, SCFP and AOFP also increased the survival rate of lipopolysaccharides stimulated PBMCs by almost two-fold compared to the negative control. A total of 240 broilers were divided into four groups as Control, SCFP 0.1% (SCFP), SCFP 0.05% + AOFP 0.05% (SAFP), and AOFP 0.1% (AOFP) groups, respectively. Each group had 20 broilers, and three replicate pens. The results showed that the addition of SCFP, SAFP, and AOFP groups did not affect the growth performances, but increased the jejunum value of villus height and villus: crypt ratio on SAFP and AOFP groups compared to the control and SCFP groups. Furthermore, adding SCFP, SAFP, and AOFP significantly reduced the number of Clostridium perfringens in ileum chyme. SCFP, SAFP, and AOFP significantly reduced the amount of interleukin-1β, inducible nitric oxide synthases, interferon-γ, and nuclear factor kappa B mRNA expression in PBMCs, especially in the AOFP group. In summary, all the SCFP, SAFP, and AOFP groups can be suggested as a functional feed additive since they enhanced villus: crypt ratio and decreased inflammation-related mRNA expression, especially for AOFP group in broilers.

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