scholarly journals Poly-β-Hydroxybutyrate (PHB) Production By Amylolytic Micrococcus sp. PG1 Isolated From Soil Polluted Arrowroot Starch Waste

2015 ◽  
Vol 19 (1) ◽  
pp. 56
Author(s):  
Sebastian Margino ◽  
Erni Martani ◽  
Andriessa Prameswara
Keyword(s):  
Carbon Source ◽  
Internal Energy ◽  
Organic Polymer ◽  
Ftir Analysis ◽  
Phb Production ◽  
Alternative Carbon Source ◽  
Arrowroot Starch ◽  
Physiological Characters

Poly-β-hydroxybutyrate (PHB) production from amylolytic Micrococcus sp. PG1. Poly-β-hydroxybutyrate(PHB) is an organic polymer, which synthesized by many bacteria and serves as internal energy. PHB ispotential as future bioplastic but its price is very expensive due to glucose usage in PHB industry. Thedevelopment of PHB production using starch as an alternative carbon source has been conducted to reducethe dependence of glucose in PHB production. In this study, amylolytic bacteria from arrowroot processingsite were screened quantitavely based on amylase specifi c activity and PHB producing ability. The result of thestudy showed that among of 24 amylolytic isolates, 12 isolates of them were able to accumulate PHB rangedfrom 0,68-11,65% (g PHB/g cdw). The highest PHB production from substrate arrowroot starch was PG1 andafter optimization resulted in increasing of PHB production up to 16,8% (g PHB/g cdw) 40 hours incubationtime. Based on morphological, biochemical and physiological characters, the PG1 isolate was identifi ed asMicrococcus sp. PG1. Result of the FTIR analysis of produced polymer by Micrococcus sp. PG1 was indicatedas poly-β- hydroxybutyrate (PHB)

scholarly journals Harnessing molasses as a low-cost carbon source for production of poly-hydroxy butyrate (PHB) using Burkholderia sp. B73 bacteria

2021 ◽  
Vol 89 (2) ◽  
Author(s):  
Diah - RATNANINGRUM ◽  
Een Sri ENDAH ◽  
Puspita LISDIYANTI ◽  
Sri PRIATNI ◽  
Vienna SARASWATY
Keyword(s):  
Carbon Source ◽  
Low Cost ◽  
Biomass Accumulation ◽  
Fermentation Medium ◽  
Small Scale ◽  
Dry Cell Weight ◽  
Wide Range ◽  
Phb Production ◽  
Alternative Carbon Source ◽  
High Production Cost

Burkholderia sp. has been reported as a poly-hydroxy-butyrate (PHB) producer. PHB is a natural polyester class with a wide range of applications in foods, medicines, and biomedicines. However, the high production cost of PHB may limit its potential. Molasses, a by-product of the sugarcane industry available abundantly, may be used as an alternative carbon source of PHB production. In this research, we aimed to evaluate PHB production by Burkholderia sp. B73 in fermentation media using molasses as an alternative carbon source. Small-scale experiments were performed in Erlenmeyer flasks on a shaker at 150 rpm and 30 °C to evaluate the best initial C/N ratio for biomass accumulation and PHB production. A set of parameters including bacterial growth, dry cell weight, yield, and FTIR spectrum of PHB were observed.  The results showed that molasses could be used to grow Burkholderia sp. B73 and the highest PHB production was obtained when a 20:1 C/N ratio of molasses was applied in the fermentation medium. In addition, when the initial pH was adjusted to 7.0, the highest PHB yield was also produced. More importantly, the use of molasses as a carbon source improved the PHB yield by nearly 2-fold compared with our previous report using a synthetic Ramsay’s minimal medium. In conclusion, the experiment results showed that molasses could be used as a low-cost carbon source for PHB production by Burkholderia sp. B73 bacteria.

Use of bacteriostatic antibiotics for the optimization of new media in view of supplementing POME as an alternative carbon source for PHA production - a statistical approach

2019 ◽  
Vol 16 ◽  
pp. 1692-1701
Author(s):  
Ponnaiah Paulraj ◽  
Harvie Anak Shukri ◽  
Vnootheni Nagiah ◽  
Nagaraja Suryadevara ◽  
Balavinayagamani Ganapathy
Keyword(s):  
Carbon Source ◽  
New Media ◽  
Statistical Approach ◽  
Alternative Carbon Source ◽  
Pha Production

Model Study To Assess Softwood Hemicellulose Hydrolysates as the Carbon Source for PHB Production inParaburkholderia sacchariIPT 101

2017 ◽  
Vol 19 (1) ◽  
pp. 188-200 ◽  
Author(s):  
Karolin Dietrich ◽  
Marie-Josée Dumont ◽  
Timothy Schwinghamer ◽  
Valérie Orsat ◽  
Luis F. Del Rio
Keyword(s):  
Carbon Source ◽  
Model Study ◽  
Phb Production

Recycling of Bakery Waste as an Alternative Carbon Source for Rhamnolipid Biosurfactant Production

2018 ◽  
Vol 22 (2) ◽  
pp. 373-384 ◽  
Author(s):  
Kaustuvmani Patowary ◽  
Moonjit Das ◽  
Rupshikha Patowary ◽  
Mohan Chandra Kalita ◽  
Suresh Deka
Keyword(s):  
Carbon Source ◽  
Biosurfactant Production ◽  
Alternative Carbon Source ◽  
Rhamnolipid Biosurfactant

Transformation of Alachlor by Microbial Communities

Weed Science ◽  
1990 ◽  
Vol 38 (4-5) ◽  
pp. 416-420 ◽  
Author(s):  
Hone L. Sun ◽  
Thomas J. Sheets ◽  
Frederick T. Corbin
Keyword(s):  
Carbon Source ◽  
Growth Medium ◽  
Basal Medium ◽  
Sole Source ◽  
Ring Cleavage ◽  
Side Chain ◽  
Microbial Culture ◽  
Mixed Microbial Culture ◽  
Alternative Carbon Source ◽  
Thin Layer Chromatographic Analysis

A mixed microbial culture able to transform alachlor at a concentration of 50 μg ml-1was obtained from alachlor-treated soil after an enrichment period of 84 days. The microbial community was composed of seven strains of bacteria. No single isolate was able to utilize alachlor as a sole source of carbon. There was no alachlor left in the enriched culture after a 14-day incubation, but only 12% of the14C-ring-labeled alachlor was converted to14CO2through ring cleavage during 14 days in the basal medium amended with alachlor as a sole carbon source. The presence of sucrose as an alternative carbon source decreased the mineralization potential of the enriched culture, but sucrose increased the mineralizing ability of a three-member mixed culture. Thin-layer chromatographic analysis showed that there were four unidentified metabolites of alachlor produced by the enriched culture. Sucrose decreased the amount of two of the four metabolites. The absence of a noticeable decline in radioactivity beyond the initial 12% suggested that the side chain of alachlor was utilized as carbon source by the enriched culture. Little difference in radioactivity between growth medium and cell-free supernatant of the growth medium suggested that the carbon in the ring was not incorporated into the cells of the degrading microorganisms.

scholarly journals Poly-β-Hydroxybutyrate Production by the Cyanobacterium Scytonema geitleri Bharadwaja under Varying Environmental Conditions

Biomolecules ◽  
2019 ◽  
Vol 9 (5) ◽  
pp. 198 ◽  
Author(s):  
Manoj K. Singh ◽  
Pradeep K. Rai ◽  
Anuradha Rai ◽  
Surendra Singh ◽  
Jay Shankar Singh
Keyword(s):  
Carbon Source ◽  
Growth Phase ◽  
Environmental Conditions ◽  
Carbon Sources ◽  
Stationary Growth Phase ◽  
Stationary Growth ◽  
Cell Weight ◽  
Dry Cell Weight ◽  
Phb Production ◽  
Dry Cell

The production of poly-β-hydroxybutyrate (PHB) under varying environmental conditions (pH, temperature and carbon sources) was examined in the cyanobacterium Scytonema geitleri Bharadwaja isolated from the roof-top of a building. The S. geitleri produced PHB and the production of PHB was linear with the growth of cyanobacterium. The maximum PHB production (7.12% of dry cell weight) was recorded when the cells of S. geitleri were at their stationary growth phase. The production of PHB was optimum at pH 8.5 and 30 °C, and acetate (30 mM) was the preferred carbon source.

Mechanical Sludge Disintegration: Providing an Alternative Carbon Source for Nutrient Removal

2007 ◽  
Vol 28 (4) ◽  
pp. 471-477 ◽  
Author(s):  
P. Kampas ◽  
S.A. Parsons ◽  
P. Pearce ◽  
S. Ledoux ◽  
P. Vale ◽  
...  
Keyword(s):  
Carbon Source ◽  
Nutrient Removal ◽  
Alternative Carbon Source
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