scholarly journals Electrogenerated Chemiluminescence for Immunoassay Applications

2021 ◽  
Vol 21 (6) ◽  
pp. 1599
Author(s):  
Isnaini Rahmawati ◽  
Irkham Irkham ◽  
Rahmat Wibowo ◽  
Jarnuzi Gunlazuardi ◽  
Yasuaki Einaga ◽  
...  

Electrogenerated chemiluminescence (ECL) has recently become one of the most prominent and well-established transducers for immunoassay techniques. ECL relates a luminophore concentration in solution with the emission of light triggered by an electrochemical stimulus. ECL immunoassay (ECLIA) performance depends on the parameters of its light generation, including the luminophore, the species that emit light called labels in ECLIA; co-reactants, which are added reagents that support the luminophore to undergo the excited state; electrodes, which are the place for the ECL reactions to take place; and the format of the immunoassay. This review discusses the behaviour of ECLIA parameters, the required instrumentations, and some important examples of detections based on ECLIA.

2011 ◽  
Vol 3 (5) ◽  
pp. 1713-1720 ◽  
Author(s):  
Hsin-Chieh Peng ◽  
Chia-Cheng Kang ◽  
Ming-Ren Liang ◽  
Chun-Yen Chen ◽  
Alexander Demchenko ◽  
...  

2017 ◽  
Vol 15 (19) ◽  
pp. 4072-4076 ◽  
Author(s):  
Qiang Fei ◽  
Xianfeng Gu ◽  
Yajing Liu ◽  
Ben Shi ◽  
Hengyan Liu ◽  
...  

New ESIPT-based BODIPYs are developed to render the NIR emissions with a large Stokes shift.


2011 ◽  
Vol 133 (44) ◽  
pp. 17738-17745 ◽  
Author(s):  
Kuo-Chun Tang ◽  
Ming-Jen Chang ◽  
Tsung-Yi Lin ◽  
Hsiao-An Pan ◽  
Tzu-Chien Fang ◽  
...  

Author(s):  
Ben O. Spurlock ◽  
Milton J. Cormier

The phenomenon of bioluminescence has fascinated layman and scientist alike for many centuries. During the eighteenth and nineteenth centuries a number of observations were reported on the physiology of bioluminescence in Renilla, the common sea pansy. More recently biochemists have directed their attention to the molecular basis of luminosity in this colonial form. These studies have centered primarily on defining the chemical basis for bioluminescence and its control. It is now established that bioluminescence in Renilla arises due to the luciferase-catalyzed oxidation of luciferin. This results in the creation of a product (oxyluciferin) in an electronic excited state. The transition of oxyluciferin from its excited state to the ground state leads to light emission.


Author(s):  
E. G. Rightor

Core edge spectroscopy methods are versatile tools for investigating a wide variety of materials. They can be used to probe the electronic states of materials in bulk solids, on surfaces, or in the gas phase. This family of methods involves promoting an inner shell (core) electron to an excited state and recording either the primary excitation or secondary decay of the excited state. The techniques are complimentary and have different strengths and limitations for studying challenging aspects of materials. The need to identify components in polymers or polymer blends at high spatial resolution has driven development, application, and integration of results from several of these methods.


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