scholarly journals The Biotransformation and Biodecolorization of Methylene Blue by Xenobiotic Bacterium Ralstonia pickettii

2021 ◽  
Vol 21 (6) ◽  
pp. 1418
Author(s):  
Adi Setyo Purnomo ◽  
Asranudin Asranudin ◽  
Didik Prasetyoko ◽  
Yulinar Dwi Nur Azizah
Keyword(s):  
Methylene Blue ◽  
Nutrient Broth ◽  
Chromophore Group ◽  
Ralstonia Pickettii ◽  
Azure A ◽  
Metabolic Products ◽  
Scientific Report ◽  
Tof Ms

The biotransformation and biodecolorization of methylene blue (MB) dye using the bacterium Ralstonia pickettii was investigated. This experiment was conducted in a nutrient broth (NB) medium after adding MB at 100 mg L–1 concentration. Approximately 98.11% of MB was decolorized after 18 h of incubation. In addition, the metabolic products detected by LC-TOF/MS were Azure A (AA), thionine, leuco-MB, and glucose-MB, which indicated the MB degradation through a reductase that attacked the heterocyclic central chromophore group present in the structure. Moreover, azure A and thionine fragments resulted from the N-demethylase enzyme that attacked the auxochrome group. Thus, this research was assumed to be the first scientific report suggesting the potential to use R. pickettii in the biodecolorization and biotransformation of dye waste, particularly MB.

Solvatochromism, dichroism and excited state dipole moment of azure A and methylene blue

2013 ◽  
Vol 179 ◽  
pp. 118-123 ◽  
Author(s):  
A. Ghanadzadeh Gilani ◽  
M. Salmanpour ◽  
T. Ghorbanpour
Keyword(s):  
Methylene Blue ◽  
Dipole Moment ◽  
Excited State ◽  
Azure A ◽  
Excited State Dipole Moment

A Study of the Binding of Methylene Blue, Thionin, Azure A, Azure B, and Azure C to DNA

Heterocycles ◽  
1979 ◽  
Vol 12 (11) ◽  
pp. 1391 ◽  
Author(s):  
Raymond N. Castle ◽  
Scott A. Burton
Keyword(s):  
Methylene Blue ◽  
Azure A ◽  
Azure B

Exploring the interaction of phenothiazinium dyes methylene blue, new methylene blue, azure A and azure B with tRNAPhe: spectroscopic, thermodynamic, voltammetric and molecular modeling approach

2017 ◽  
Vol 19 (9) ◽  
pp. 6636-6653 ◽  
Author(s):  
Puja Paul ◽  
Soumya Sundar Mati ◽  
Subhash Chandra Bhattacharya ◽  
Gopinatha Suresh Kumar
Keyword(s):  
Methylene Blue ◽  
Molecular Modeling ◽  
Small Molecules ◽  
Azure A ◽  
Modeling Approach ◽  
Azure B ◽  
Rna Targeting

RNA targeting by small molecules.

The photodynamic efficiency of phenothiazinium dyes is aggregation dependent

2017 ◽  
Vol 41 (23) ◽  
pp. 14438-14443 ◽  
Author(s):  
Claudia M. L. Francisco ◽  
José M. L. A. Gonçalves ◽  
Bruno S. Brum ◽  
Thabata P. C. Santos ◽  
Adriana Lino-dos-Santos-Franco ◽  
...  
Keyword(s):  
Methylene Blue ◽  
Azure A ◽  
Azure B

Effectiveness increased in the order of Azure A < Azure B < Methylene Blue while aggregation increased in the order of Methylene Blue < Azure B < Azure A.

Purification of Thionin, Azure A, Azure B and Methylene Blue

1963 ◽  
Vol 38 (2) ◽  
pp. 75-84 ◽  
Author(s):  
C. Nerenberg ◽  
Roland Fischer
Keyword(s):  
Methylene Blue ◽  
Azure A ◽  
Azure B

scholarly journals Occurrence of Pasteurellosis and Newcastle Disease in indigenous chickens in Sirajgonj district

2014 ◽  
Vol 11 (2) ◽  
pp. 97-105
Author(s):  
SMSH Belal
Keyword(s):  
Methylene Blue ◽  
Newcastle Disease ◽  
Clinical Signs ◽  
Nutrient Broth ◽  
Blood Agar ◽  
Blue Staining ◽  
Isolation And Identification ◽  
Agar Media ◽  
Staining Techniques ◽  
Heart Blood

A study was carried out on indigenous layer chickens to know occurrence of Avian Pasteurellosis (AP) and Newcastle disease (ND) in Sirajgonj during the period from January/2012 to December/2013. The clinical signs showed before death was recorded by taking history and the birds were subjected to post mortem examination. In addition to the clinical and necropsy findings, ND was detected by Anigen® rapid antigen detection kit. The AP was confirmed by isolation and identification of Pasteurella (P.) multocida from liver, spleen and heart samples. The P. multocida was found to grow on nutrient broth, nutrient agar, blood agar and Eosin Methylene Blue agar where it produced whitish, opaque, round, flat, translucent colonies. It produced turbidity on nutrient broth. The organism was not found to grow on MacConkey agar media and did not cause hemolysis on blood agar media. The impression smear of liver and heart blood were stained by Gram’s staining, Leishman staining and Methylene blue staining techniques to detect P. multocida. P. multocida organism was also found to fermnt dextrose, lactose and mannitol with the production of only acid but did not ferment maltose and lactose. P. mutocida was found non-motile, indole positive and urease negative. On triple sugar iron test it produced H2S and fermented only glucose. It was found negative to both methyl red test and Voges Proskauer test. Out of 360 birds tested 59.72% (215) was AP positive and 40.28% was ND positive. Age, Sex, season and mortality due to other diseases were not considered.DOI: http://dx.doi.org/10.3329/bjvm.v11i2.19123 Bangl. J. Vet. Med. (2013). 11 (2): 97-105

A SEM and Light Microscope Study of the Epidermal Leaf Structures of the Carnivorous Plant, Sarracenia purpurea, L.

1971 ◽  
Vol 29 ◽  
pp. 358-359
Author(s):  
B. J. Panessa ◽  
J. F. Gennaro
Keyword(s):  
Methylene Blue ◽  
Toluidine Blue ◽  
Outer Surface ◽  
Microscope Study ◽  
Light Microscope ◽  
Carnivorous Plant ◽  
Sarracenia Purpurea ◽  
Inner Surface

Tissue from the hood and sarcophagus regions were fixed in 6% glutaraldehyde in 1 M.cacodylate buffer and washed in buffer. Tissue for SEM was partially dried, attached to aluminium targets with silver conducting paint, carbon-gold coated(100-500Å), and examined in a Kent Cambridge Stereoscan S4. Tissue for the light microscope was post fixed in 1% aqueous OsO4, dehydrated in acetone (4°C), embedded in Epon 812 and sectioned at ½u on a Sorvall MT 2 ultramicrotome. Cross and longitudinal sections were cut and stained with PAS, 0.5% toluidine blue and 1% azure II-methylene blue. Measurements were made from both SEM and Light micrographs.The tissue had two structurally distinct surfaces, an outer surface with small (225-500 µ) pubescent hairs (12/mm2), numerous stoma (77/mm2), and nectar glands(8/mm2); and an inner surface with large (784-1000 µ)stiff hairs(4/mm2), fewer stoma (46/mm2) and larger, more complex glands(16/mm2), presumably of a digestive nature.

Sign in / Sign up

Export Citation Format

Share Document