scholarly journals EVALUATION AND OPTIMIZATION OF ANTIOXIDANT POTENTIALITY OF XYLARIA FEEJEENSIS HMJAU22039

2016 ◽  
pp. 269 ◽  
Author(s):  
Durga Pallavi Rebbapragada ◽  
Rajagopal Kalyanaraman
Keyword(s):  
Antioxidant Activity ◽  
Yeast Extract ◽  
Phenolic Content ◽  
Radical Scavenging ◽  
Basal Medium ◽  
Tectona Grandis ◽  
Total Phenolic ◽  
Dpph Radical ◽  
High Antioxidant Activity ◽  
Dpph Radical Scavenging

<p>ABSTRACT<br />Objective: Antioxidants neutralize free radicals generated in the human body and prevent them from causing damage. The present study is aimed to<br />evaluate and optimize the antioxidant activity of Xylaria feejeensis HMJAU22039 an endophyte isolated from Tectona grandis.<br />Methods: The antioxidant potential was measured by 1-1diphenyl-2-picryl-hydrazil (DPPH) radical scavenging assay and nitric oxide (NO) scavenging<br />assay. Total phenolic content (TPC) of the samples was measured by Folin–Ciocalteu reagent based assay, and the values were obtained from the<br />regression equation: y = 0.006141x + 0.157733.<br />Results: Potato dextrose yeast extract broth medium was selected as the basal medium as it reported a high antioxidant activity. The basal medium<br />was optimized with 2% dextrose, 0.2% yeast extract, and 200 g/L potato infusion. Dextrose and yeast extract were selected as carbon and nitrogen<br />sources, respectively, as they reported high antioxidant activity. The antioxidant activity and phenolic content are highest at static condition<br />(18.14 mg/g). Incubation temperature of 30°C and pH of the initial medium at 6 were found to be optimum conditions for high antioxidant activity. An<br />incubation period of 20 days reported the highest antioxidant activity and phenolic content. Methanol extract recorded high antioxidant activity with<br />a DPPH (73.86%) followed by ethyl acetate and chloroform fractions.<br />Conclusion: The study highlights the importance of different physiochemical parameters in the production of secondary metabolites having<br />antioxidant properties. The results reveal a significant positive correlation between DPPH radical scavenging assay, NO scavenging assay, and TPC.<br />Keywords: 1-1diphenyl-2-picryl-hydrazil, Total phenolic content, Endophytic fungus, Tectona grandis.</p>

scholarly journals Total Phenolic Content and Antioxidant Activity of Salvia spp. Exudates

2012 ◽  
Vol 7 (2) ◽  
pp. 1934578X1200700 ◽  
Author(s):  
Laura Giamperi ◽  
Anahi Bucchini ◽  
Angela Bisio ◽  
Emanuela Giacomelli ◽  
Giovanni Romussi ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Total Phenolic Content ◽  
Phenolic Content ◽  
Radical Scavenging ◽  
Natural Antioxidants ◽  
Total Phenolic ◽  
Dpph Radical ◽  
Radical Scavenging Capacity ◽  
Scavenging Capacity ◽  
Dpph Radical Scavenging

The total phenolic content and antioxidant activity of 6 Salvia spp. exudates were measured to find new potential sources of natural antioxidants. Total phenolic content was assessed by a modified Prussian blue method, and the antioxidant activity by two methods: 1,1- diphenyl-2-picryl- hydrazyl (DPPH) radical scavenging capacity assay and lipoxygenase inhibitory assay. The total phenolic content ranged between 1.3 μg/mg DW ( S. fallax) and 74.0 μg/mg DW ( S. cacaliaefolia). In the DPPH test, S. cacaliaefolia was more effective than BHT, while in the inhibition of lipid peroxidation all the extracts presented good antioxidant capacity.

PENGARUH METODE EKSTRAKSI TERHADAP KADAR FENOLAT TOTAL DAN AKTIVITAS ANTIOKSIDAN DAUN SALAM (Syzygium polyanthum (Wight) Walp.)

2017 ◽  
Vol 2 (2) ◽  
pp. 53 ◽  
Author(s):  
Verawati Verawati ◽  
Dedi Nofiandi ◽  
Petmawati Petmawati
Keyword(s):  
Antioxidant Activity ◽  
Extraction Method ◽  
Phenolic Content ◽  
Radical Scavenging ◽  
Extraction Methods ◽  
Total Phenolic ◽  
Dpph Radical ◽  
Percolation Method ◽  
Dpph Radical Scavenging ◽  
One Way Anova

<p><em>The research about influence of extraction method on phenolic content and antioxidant activity of Bay leaves (Syzygium polyanthum (Wight) Walp) has been conducted.  Extraction methods used were maceration, percolation and soxhletation with ethanol 70% used as solvent extraction.  Total phenolic level were determined by using Folin Ciocalteu method whereas antioxidant activity with DPPH radical scavenging method. . The highest extractive value was obtained from percolation method at 59.8% followed by maceration at 44.4% and soxhletation 22%. The highest levels of phenolic content was obtained from percolation (103,91mg/g), followed by soxhletation (72.80 mg/g) and maceration (69.76 mg/g). The antioxidant activity was shown by IC<sub>50</sub> values, the percolation method amounted to 49.67 µg/ml; soxhletation 49.98 µg/ml and maceration 35.05 µg/ml. Based on the results of statistical analysis SPSS 17 using one-way ANOVA known that the extraction method significantly affect phenolic content and antioxidant activity of the extract of bay leaves.</em></p>

scholarly journals Evaluation of antioxidant properties of phycobiliproteins and phenolic compounds extracted from Bangia atropurpurea

2018 ◽  
Vol 14 (2) ◽  
pp. 289-297 ◽  
Author(s):  
Rajalakshmi Punampalam ◽  
Kong Soo Khoo ◽  
Nam Weng Sit
Keyword(s):  
Antioxidant Activity ◽  
Phenolic Compounds ◽  
Phenolic Content ◽  
Radical Scavenging ◽  
Total Phenolic ◽  
Dpph Radical ◽  
Soluble Extract ◽  
Recovery Yield ◽  
Bangia Atropurpurea ◽  
Dpph Radical Scavenging

Bangia atropurpurea is a freshwater red filamentous alga. In this study, we report on the recovery yield of purified phycobiliproteins, total phenolic content (TPC) and their antioxidant capacity. B. atropurpurea suspended in phosphate buffer (pH 7.2), saturated with ammonium sulphate and further isolated R-phycoerythrin and phycocyanin from phycobilisome structure by gel filtration with Sephadex G-200. Phenolic compounds extracted by variable polarity solvents to exhibit optimum yield. Folin–Ciocalteu method was used to determine the TPC, while 1, 1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging and ferric-reducing antioxidant power (FRAP) assays were used to quantitate the antioxidant activity of phycobiliproteins and phenolic compounds.  B. atropurpurea predominantly contains R-phycoerythrin 54.80 % of the total protein extracted at purity ratio A562 to A280 of 4.45 while, phycocyanin displayed 16 % recovery yield at purity ratio A615 to A280 of 2.18. The purified R-phycoerythrin exhibited better antioxidant activity than phycocyanin extract and BHT synthetic antioxidant by exhibiting (IC50%=7.66 ±0.81µg/mL) and 54.74 ± 0.11 mg GAE/1g extract for, DPPH scavenging and FRAP assay, respectively. The sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) of purified R-phycoerythrin and phycocyanin proteins have showed apparent single band at molecular weight of 19.6 kDa and 17.5 kDa, respectively. The methanol-soluble extract displayed the utmost TPC, 80.97±0.53 mg GAE/1g extract compared to phenolic content in other organic solvents used for extraction. In accordance with TPC, methanol-soluble extract exhibited as the most effective DPPH radical scavenging and ferric reduction, (IC50%=14.63±0.79 µg/mL) and 37.79±0.27 mg GAE/1g extract, respectively. The presence of R-phycoerythrin, phycocyanin and phenolic compounds in an array, amplifies the antioxidant property of B. atropurpurea.

scholarly journals Antioxidant Activity and Total Phenolic Contents of Three Bupleurum Taxa

2014 ◽  
Vol 9 (4) ◽  
pp. 1934578X1400900
Author(s):  
Hyeusoo Kim ◽  
Sea Hyun Kim ◽  
Kyeong Won Yun
Keyword(s):  
Antioxidant Activity ◽  
Phenolic Content ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
The Other ◽  
Total Phenolic ◽  
Scavenging Activity ◽  
Dpph Radical ◽  
Dpph Radical Scavenging Activity ◽  
Dpph Radical Scavenging

Bupleuri Radix (Bupleurum spp. root) is one of the most important crude drugs in Korea, China and Japan. We investigated the total phenolic content and antioxidant activity of three Bupleurum taxa (B. falcatum, B. falcatum ‘Mishima’ and B. latissimum). The highest total phenolic content was found in B. latissimum (18.6±1.7 mg/g) and the least in B. falcatum ‘Mishima’ (9.4±0.5 mg/g). The ethyl acetate fractions obtained from B. falcatum and B. falcatum ‘Mishima’ showed higher DPPH radical scavenging activity than the other fractions. In the case of B. latissimum, the DPPH radical scavenging activity of the diethyl ether fraction was higher than that of the other fractions. These results suggest that the three Bupleurum plants may be used as a food additive as a natural antioxidant.

scholarly journals Phenolics Content, Antioxidant Activity and Color of Green Walnut Extracts for Preparing Walnut Liquor

2014 ◽  
Vol 42 (2) ◽  
pp. 551-555 ◽  
Author(s):  
Sina COSMULESCU ◽  
Ion TRANDAFIR ◽  
Violeta NOUR ◽  
Mira IONICA ◽  
Felicia TUTULESCU
Keyword(s):  
Antioxidant Activity ◽  
Phenolic Content ◽  
Total Phenolics ◽  
Radical Scavenging ◽  
Total Phenolic ◽  
Dpph Radical ◽  
Solvent Concentration ◽  
Sugar Addition ◽  
Phenolics Content ◽  
Dpph Radical Scavenging

Different extracts prepared from green fruits of ‘Sibisel 44’ walnut cultivar were investigated for their total phenolics and flavonoids, antioxidant activity, individual phenolics content and colour component. Extracts were prepared using three variants, to analyze the effect of solvent concentration and sugar addition on properties of green walnuts extracts. The antioxidant activity of alcoholic extracts was investigated by the 2,2'-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging method while total phenolic content was determined by using the Folin-Ciocalteu assay. Seventeenindividual phenolics were detected and quantified by using HPLC in walnut green fruit extracts. For total phenolics and flavonoids extraction from green walnut fruits 70% ethanol was more efficient. Highest concentration was found in samples with 70% ethanol for gallic, vanillic, chlorogenic, caffeic, syringic, salicylic, ellagic acids, juglone, catechin, epicatechin, myricetin, and quercetin. The use of 40% ethanol led to a significant increase of ferulic acid and rutin content, while the greatest amount of rutin was extracted using 40% ethanol and sugar.

scholarly journals Determination of Phenolic Compounds, Procyanidins, and Antioxidant Activity in Processed Coffea arabica L. Leaves

Foods ◽  
2019 ◽  
Vol 8 (9) ◽  
pp. 389 ◽  
Author(s):  
Samuchaya Ngamsuk ◽  
Tzou-Chi Huang ◽  
Jue-Liang Hsu
Keyword(s):  
Antioxidant Activity ◽  
Total Phenolic Content ◽  
Phenolic Content ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Total Phenolic ◽  
Dpph Radical ◽  
Dpph Radical Scavenging Activity ◽  
Dpph Radical Scavenging ◽  
Coffea Arabica L

The effects of dry processing and maturity on antioxidant activity, total phenolic content, total procyanidins, and identity of phenolic compounds in coffee leaves were evaluated. Fresh coffee leaves were tray-dried at 40 °C for 8 h before total phenolic content, total procyanidins, and antioxidant activity were analyzed. The drying process significantly (p < 0.05) improved the release of total phenolic content and total procyanidins compared with the fresh leaves. The results showed that the young leaves exposed to drying processes had the highest total phenolic content, total procyanidins, and DPPH radical scavenging activity. Therefore, the effect of different drying temperatures (30, 40, and 50 °C) in the young leaves were further analyzed. The results indicated that DPPH radical scavenging activity, total phenolic content, and total procyanidins were increasingly generated when exposed to an increase in drying temperatures, whereby the highest bioactivity was evident at 50 °C. The DPPH radical scavenging activity of the coffee leaf teas was significantly correlated with total phenolic content and total procyanidins. Identification of Coffea arabica L. bioactive compounds by LC-MS showed the presence of catechin or epicatechin, mangiferin or isomangiferin, procyanidin B, caffeoylquinic acids (CQA), caffeine, quercetin-3-O-glucoside, procyanidin C, rutin, and 3,4-diCQA. Coffea arabica L. leaf tea was confirmed to be a potential functional food rich in phenolic compounds with strong antioxidant activity.

scholarly journals Antioxidant Activities, Total Phenolic Content and Colour Parameters in the Aqueous Extracts of Avocado, Banana and Papaya Leaves

2021 ◽  
Vol 19 (01) ◽  
pp. 137-142
Author(s):  
VIJAYA KUMAR GOPALAN NIRVESHA NAIR ◽  
SEOK TYUG TAN
Keyword(s):  
Phenolic Content ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Total Phenolic ◽  
Scavenging Activity ◽  
Dpph Radical ◽  
Dpph Radical Scavenging Activity ◽  
Colour Parameters ◽  
Banana Leaves ◽  
Dpph Radical Scavenging

Literature has consistently reported that horticultural wastes including leaves, skin, stones and seeds contain substantial amounts of bioactive compounds. Therefore, this study aims to evaluate antioxidant activity, Total Phenolic Content (TPC) and colour parameters in avocado, banana, and papaya leaves. Antioxidant activity of the leaves was determined using Trolox Equivalent Antioxidant Capacity (TEAC) and DPPH radical scavenging assays, whereas TPC was evaluated using Folin-Ciocalteu assay. Data analysis was carried out using SPSS version 25 of triplicate determinations. Mean differences among the fruit leaves extracts were determined using One way-ANOVA, while the correlations between the studied components were by the Pearson's Correlation Coefficient Test. The colour of the extracts was determined using a colour picker software. The TEAC values were in the range of 332.30 ± 18.04 µg Trolox/g D.W. (avocado leaves) to 12217.71 ± 18.04 µg Trolox/g D.W. (banana leaves) while the DPPH radical scavenging activity was from 10.07 ± 3.89% (banana leaves) to 86.70 ± 0.26 % (avocado leaves). Besides, TPC was from 871.33 ± 38.35 µg GAE/g D.W. (papaya leaves) to 1199.08 ± 6.00 µg GAE/g D.W. (avocado leaves). The hue values were from 19º in avocado leaves extract to 37º in banana leaves extract. Results from Pearson's Correlation Coefficient Test revealed that there were no significant correlations between the studied assays. Avocado leaves had the highest DPPH radical scavenging activity and TPC among the three extracts. Findings derived from the present study could be exploited in nutraceuticals formulation.

scholarly journals Effects of Solvents on Total Phenolic Content and Antioxidant Activity of Ginger Extracts

2020 ◽  
Author(s):  
Dessie Ezez ◽  
Molla Tefera
Keyword(s):  
Total Phenolic Content ◽  
Phenolic Content ◽  
Antioxidant Activities ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Acetone Extract ◽  
Total Phenolic ◽  
Dpph Radical ◽  
Dpph Radical Scavenging Activity ◽  
Dpph Radical Scavenging

Abstract Ginger (Zingiber officinale) is a popular spice which used for the treatment of different gastrointestinal and inflammatory discomfort. In the present study, the total phenolic content (TPC) and antioxidant activity of ginger extract using four solvents (ethanol, methanol, acetone and ethyl acetate) were determined. Among the four solvents, methanol extract showed that the maximum phenolic (1183.813 mg GAE/100 g at Ayikel and 1022.409 mg GAE/100 g at Mandura) and the least were found in acetone extract (748.865 mg GAE/100 g at Ayikel and 690.152 mg GAE/100 g at Mandura). In addition, the highest DPPH radical scavenging activity (84.868% at Ayikel and 82.883% at Mandura) was observed in methanol. However, acetone showed the least DPPH radical scavenging activity (73.864% at Ayikel and 70.597% at Mandura). Antioxidant activities of ginger extracts were also expressed as IC50 values and acetone extract has maximum IC50 value (0.654 and 0.812 mg/mL) followed by ethyl acetate and ethanol, while the lowest for methanol extracts (0.481 and 0.525 mg/mL). The result of this study showed that extraction solvents significantly affected the total phenolic content and antioxidant activities of ginger. Thus, ginger can be regarded as promising candidates for natural sources of antioxidants with high value of phenolic contents.

scholarly journals Hypericum perforatum Mikrokapsüllerinin Ayran (İçilebilir Yoğurt) Üretiminde Kullanımı

2021 ◽  
Vol 9 (11) ◽  
pp. 2013-2021
Author(s):  
Fadime Seyrekoğlu ◽  
Hasan Temiz
Keyword(s):  
Phenolic Compounds ◽  
Sensory Analysis ◽  
Phenolic Content ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Total Phenolic ◽  
Scavenging Activity ◽  
Dpph Radical ◽  
Dpph Radical Scavenging Activity ◽  
Dpph Radical Scavenging

H. perforatum, which is widely used in traditional medicine due to its bioactive compounds was extracted with ethanol-water (3:7). The extract was encapsulated with maltodextrin and gum arabic in a spray dryer in order to protect the phenolic compounds in its structure. Different amounts of microcapsules were added to our traditional drink, i.e. ayran (drinking yoghurt). The total phenolic content and DPPH radical scavenging activity of the microcapsules, extract of HP, and ayran samples were determined. The amount of total phenolic compounds in the microcapsule provided a superior effect than the extract. The ayran samples were supplemented with 2%, 3%, 4%, 5% and 6% of H. perforatum (HP) microcapsules and it is observed that total phenolic content (TPC) and DPPH radical scavenging activity indicated an increase with concentration. TPC and DPPH activity were determined as 256.94 mg GAE / 100mL and 78.05% for 6% of HP microcapsules supplemented samples. As a result of the sensory analysis, ayran samples which supplemented with 4% of HS microcapsule gained the highest scores by the panellists and received more appreciation than the control group. According to sensory analysis, HP4 (ayran produced with 4% HP added microcapsule) sample was determined as the best sample, while the HP6 (Ayran produced with 6% HP added microcapsule) sample had the highest scores in terms of DPPH scavenging activity and TPC results. The overall results of this study revealed that 4% HP supplemented ayran can be produced with its increased health benefits and desirable properties. In this study, the use of H. perforatum microcapsules in ayran, its effect on antioxidant and phenolic components, the usage rates and acceptability of microcapsules were investigated.

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