scholarly journals ANTITUBERCULOSIS ACTIVITY OF EXTRACT AND FRACTIONS OF TINOSPORA CRISPA AGAINST MYCOBACTERIUM TUBERCULOSIS H37RV USING MYCOBACTERIA GROWTH INDICATOR TUBE AND AGAR PROPORTION METHOD

2018 ◽  
Vol 11 (3) ◽  
pp. 132
Author(s):  
Retno Wahyuningrum ◽  
Ritmaleni Ritmaleni ◽  
Tatang Irianti ◽  
Subagus Wahyuono ◽  
Takushi Kaneko
Keyword(s):  
Mycobacterium Tuberculosis ◽  
Ethanolic Extract ◽  
Multidrug Resistant ◽  
Drug Candidates ◽  
Mycobacterium Tuberculosis H37rv ◽  
Indicator Tube ◽  
Resistant Tuberculosis ◽  
Lowenstein Jensen ◽  
Proportion Method ◽  
Growth Indicator

 Objective: The increasing incidence of multidrug-resistant tuberculosis (TB) has created a need to discover a new anti-TB drug candidates. The aim of this study was to screen extract and fractions of Tinospora crispa for activity against Mycobacterium tuberculosis H37Rv.Methods: The dried and pulverized T. crispa stem was extracted by maceration method using ethanol (96%). The anti-TB activity was carried out using mycobacteria growth indicator tube (MGIT) system and agar proportion method with Lowenstein–Jensen (LJ) medium.Result: The result of this study showed that ethanolic extract and fractions of T. Crispa did not exhibit anti-TB activity in the range of 100–1000 μg/ml with MGIT method, while with agar proportion method, there were M. tuberculosis colonies growth on the LJ containing 1000 μg/ml extract slants.Conclusion: The tested extract and fractions of T. crispa have no anti-TB activity against M. tuberculosis until 1000 μg/ml.

scholarly journals Occurrence ofrpoBMutations in Isoniazid-Resistant but Rifampin-Susceptible Mycobacterium tuberculosis Isolates from Germany

2013 ◽  
Vol 58 (1) ◽  
pp. 590-592 ◽  
Author(s):  
Sönke Andres ◽  
Doris Hillemann ◽  
Sabine Rüsch-Gerdes ◽  
Elvira Richter
Keyword(s):  
Mycobacterium Tuberculosis ◽  
Susceptibility Testing ◽  
Drug Susceptibility ◽  
Drug Susceptibility Testing ◽  
Content Type ◽  
Indicator Tube ◽  
Lowenstein Jensen ◽  
Proportion Method ◽  
Rifampin Resistance ◽  
Growth Indicator

ABSTRACTFour out of 143 phenotypically isoniazid-resistant but rifampin-susceptibleMycobacterium tuberculosisstrains that were isolated from patients in Germany in 2011 had mutations in the rifampin resistance-determining region ofrpoB. After performing drug susceptibility testing (DST) with two methods, the proportion method on Löwenstein-Jensen medium and using the Bactec 960 Mycobacteria Growth Indicator Tube system, we conclude that the two methods are equally reliable for phenotypic DST and MIC determination.

Green Synthesis of Gold Nanoparticles Using Different Leaf Extracts of Ocimum gratissimum Linn for Anti-tubercular Activity

2019 ◽  
Vol 9 (2) ◽  
pp. 146-157
Author(s):  
Arti Gupta ◽  
Sonia Pandey ◽  
Bharat Variya ◽  
Shailesh Shah ◽  
Jitendra Singh Yadav
Keyword(s):  
Gold Nanoparticles ◽  
Mycobacterium Tuberculosis ◽  
Ethanolic Extract ◽  
Leaf Extracts ◽  
Ocimum Gratissimum ◽  
Mycobacterium Tuberculosis H37rv ◽  
Vis Spectroscopy ◽  
Lowenstein Jensen ◽  
Vitro Model

Background: Tuberculosis is a greatest threat to human health. It requires urgent need to seek new devise alternate strategies and ant-tubercular compounds. In the present scenario, Nonmaterias, have opened new avenues in medicine, diagnosis and therapeutics. Objective: In view of this, the current study aims to synthesize gold nanoparticles and determine its efficacy to inhibit Mycobacterium tuberculosis. Methods: Gold nanoparticles (GNPs) synthesized from medicinal plant, such as Ocimum gratissimum linn, were tested against Mycobacterium tuberculosis (H37RV strain). Gold nanoparticles were characterized by UV-Vis spectrophotometer, FTIR, SEM and TEM. TEM results revealed that the GNPs were found spherical in structure and around 10-25 nm in diameter. UV-Vis spectroscopy exhibited an absorption peak at 348 nm. Fourier transform infra-red spectroscopy showed the GNPs have coated with phytoconstituents (terpenoids) that indicate the role of bio-molecules responsible for efficient stabilization and capping of the gold nanoparticles. In vitro model was designed to determine minimum inhibitory concentration (MIC) of each sample by Lowenstein Jensen (LJ) slope method. Results: The results showed that the presence of ursolic acid in ethanolic and hydroalcoholic extracts was found to be 2.89% and 1.97%, respectively. GNPs of ethanolic and hydroalcoholic exhibited anti-tubercular activity, with MIC 2.5 µg/ml and 20 µg/ml, respectively. While ethanolic and hydroalcoholic extracts showed such activity at concentrations 50 µg/ml and 75 µg/ml, respectively. Conclusion: GNPs synthesized from ethanolic extract showed profound efficiency to kill mycobacteria. As in this method no chemical reagents were used, the synthesized gold nanoparticles have potential for biological applications. There is an urgent need to further development of nano-antibiotic for tuberculosis.

scholarly journals Evaluation of a Rapid Differentiation Test for Mycobacterium Tuberculosis from other Mycobacteria by Selective Inhibition with p-nitrobenzoic Acid using MGIT 960

2010 ◽  
Vol 2 (02) ◽  
pp. 089-092 ◽  
Author(s):  
Babita Sharma ◽  
Nita Pal ◽  
Bharti Malhotra ◽  
Leela Vyas
Keyword(s):  
Mycobacterium Tuberculosis ◽  
Selective Inhibition ◽  
Clinical Samples ◽  
Biochemical Tests ◽  
Mycobacterium Tuberculosis H37rv ◽  
Nitrobenzoic Acid ◽  
Indicator Tube ◽  
Heat Stable ◽  
Lowenstein Jensen ◽  
Differentiation Test

ABSTRACTTuberculosis is caused by Mycobacterium tuberculosis (M.tb) as well as Non-tubercular mycobacterium (NTM) with similar clinical presentation. Infections due to NTM are reported to have increased in the past few years. Growth of M.tb is inhibited by p-Nitrobenzoic acid (PNB), whereas, NTM are resistant. One hundred and nine isolates from various clinical samples were identified up to species level by their growth rate, pigmentation, and a battery of biochemical tests, including niacin accumulation, nitrate reduction, and heat-stable catalase (68°C) reactions. Para-nitrobenzoic acid (PNB) inhibition test was performed to differentiate between M.tb and NTM. PNB was added to the Lowenstein-Jensen (LJ) medium and BACTECTM MIGIT (Mycobacteria Growth Indicator Tube)960 medium to a final concentration of 500 μg/ml. All the M.tb isolates, including Mycobacterium tuberculosis H37Rv (standard strain), were inhibited by PNB on both LJ and MGIT 960. Of the NTM isolates, all were resistant to PNB on MGIT 960 and on LJ PNB, except one isolate of Mycobacterium marinum that was resistant to MGIT 960 PNB, but was susceptible to LJ PNB. The reporting time for M.tb ranged from 4–11 days (median 5.9 days) by MGIT 960 and for NTM it was 2–10 days with an average of 4.5 days. This study was carried out to establish the accuracy and efficiency of MGIT 960 PNB and to differentiate between M.tb and NTM.

scholarly journals Antituberculosis Activity of Brotowali (Tinospora crispa) Extract and Fractions against Mycobacterium tuberculosis using Microplate Alamar Blue Assay Method

2017 ◽  
Vol 22 (2) ◽  
pp. 124
Author(s):  
Retno Wahyuningrum ◽  
Ritmaleni Ritmaleni ◽  
Tatang Irianti ◽  
Subagus Wahyuono ◽  
Takushi Kaneko ◽  
...  
Keyword(s):  
Mycobacterium Tuberculosis ◽  
Pathogenic Bacteria ◽  
Ethanolic Extract ◽  
Multidrug Resistant ◽  
Alamar Blue Assay ◽  
Assay Method ◽  
Drug Candidate ◽  
Antituberculosis Activity ◽  
Alamar Blue ◽  
Resistant Tuberculosis

Tuberculosis (TB), in which caused by pathogenic bacteria, Mycobacterium tuberculosis, has become the major causes of death among all of infectious diseases. The increasing incidence of multidrug-resistant tuberculosis (MDR-TB) and extensively drug-resistant tuberculosis (XDR-TB) has created a need to discover a new antituberculosis drug candidate. The aim of this study was to screen extract and fractions of Tinospora crispa for activity against Mycobacterium tuberculosis H37Rv using the Microplate Alamar Blue Assay (MABA) method. T. crispa extract was prepared by maceration in ethanol (96%) and antituberculosis activity was carried out using MABA method. The result of this study showed that ethanolic extract of T. crispa exhibit antituberculosis activity with minimum inhibition concentration of 12.5 mg/ml.

scholarly journals Effect of Piper nigrum (Linn.) seeds extract and second line anti-tuberculosis drugs on a few Mycobacterium tuberculosis strains

2021 ◽  
Vol 13 (1) ◽  
pp. 402-406
Author(s):  
G. V. Zodape ◽  
S. N. Dharmashale ◽  
V. S. Gaikwad
Keyword(s):  
Mycobacterium Tuberculosis ◽  
In Vitro Study ◽  
Ethanolic Extract ◽  
Drug Susceptibility ◽  
Drug Susceptibility Testing ◽  
Piper Nigrum ◽  
Amino Salicylic Acid ◽  
Lowenstein Jensen ◽  
Proportion Method

Piper nigrum (Linn.) belonging to the family Piperaceae have been reported for its multitudinous medicinal values. The present study was undertaken to examine the direct effect of Ethionamide (ETH), Para amino salicylic acid (PAS), ethanolic extracts of P. nigrum on Mycobacterium tuberculosis (MTB) strain H37Rv and Multi drug-resistant (MDR)-strains-12, 19 and 21. The proportion method was used to detect the anti-mycobacterial drug susceptibility testing for mycobacteria using Lowenstein Jensen (LJ) medium. It was found that P. nigrum does not interfere with single or in the combination of both ETH and PAS showing the bioenhancer activity. In vitro study of ethanolic extract of P. nigrum observed that the extract inhibited the growth of H37Rv strains and MDR strains-12, MDR strains 19, and MDR strains 21. The present results will pave new avenues to find a new medicine that possesses P. nigrum alone or in combination with drugs to combat MDR-strains controlling tuberculosis.

Sign in / Sign up

Export Citation Format

Share Document