scholarly journals TRIGONELLA FOENUM-GRAECUM L. EXHIBITS ESTROGENIC EFFECT THROUGH PRESENILIN 2 GENE EXPRESSION IN THE BREAST CANCER CELL LINE MCF-7

2019 ◽  
Vol 12 (1) ◽  
pp. 438
Author(s):  
Kurnia Agustini ◽  
Michael Wink ◽  
Wahono Sumaryono ◽  
Frans Suyatna ◽  
Nurjati Chairani Siregar
Keyword(s):  
Breast Cancer ◽  
Gene Expression ◽  
Cancer Cell Line ◽  
Active Fraction ◽  
Rt Pcr ◽  
Fenugreek Seeds ◽  
Trigonella Foenum Graecum ◽  
Presenilin 2 ◽  
Trigonella Foenum Graecum L ◽  
Mcf 7

Objective: The objective of this study is to investigate the estrogenic and antiestrogenic activity of Fenugreek seeds, Trigonella foenum-graecum L. in the estrogen-dependent breast cancer cell line, MCF-7, including its effect on the expression of estrogen-dependent presenilin 2 (pS2) gene.Methods: An activity guided fractionation was carried out with extracts from fenugreek seeds in MCF-7 cells. Cytotoxic activity assays were conducted with 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium (MTT) assay. Most fractions were also tested also tested in media with estradiol 10 nM We also analysed the expression of pS2 gene. For the analysis of pS2 gene expression we employed PCR primers for pS2 and for β-actin as a housekeeping gene using real-time polymerase chain reaction (RT-PCR).Results: Based on cytotoxic activity assay in MCF-7, the active fractions are ethyl acetic fraction and its phases ethyl acetic (EA) 2 and EA 2.2. The most active fraction was EA 2.2 (IC50=27.129 ppm), which exhibited a biphasic effect; at low concentrations, it stimulated the growth, and at high concentrations it showed strong cytotoxic effects. EA2.2 fraction in concentration 20 ppm, also could induce pS2 gene expression in media with and without estrogen.Conclusion: The most active fraction was the ethyl acetate phase and further subfractions. The most active fraction also induced the expression of pS2 gene which was studied by RT-PCR.

scholarly journals TRIGONELLA FOENUM-GRAECUM L. EXHIBITS ESTROGENIC EFFECT THROUGH PRESENILIN 2 GENE EXPRESSION IN THE BREAST CANCER CELL LINE MCF-7

2019 ◽  
Vol 12 (1) ◽  
pp. 438
Author(s):  
Michael Wink ◽  
Wahono Sumaryono ◽  
Frans Suyatna ◽  
Nurjati Chairani Siregar
Keyword(s):  
Breast Cancer ◽  
Gene Expression ◽  
Cancer Cell Line ◽  
Active Fraction ◽  
Rt Pcr ◽  
Fenugreek Seeds ◽  
Trigonella Foenum Graecum ◽  
Presenilin 2 ◽  
Trigonella Foenum Graecum L ◽  
Mcf 7

Objective: The objective of this study is to investigate the estrogenic and antiestrogenic activity of Fenugreek seeds, Trigonella foenum-graecum L. in the estrogen-dependent breast cancer cell line, MCF-7, including its effect on the expression of estrogen-dependent presenilin 2 (pS2) gene.Methods: An activity guided fractionation was carried out with extracts from fenugreek seeds in MCF-7 cells. Cytotoxic activity assays were conducted with 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium (MTT) assay. Most fractions were also tested also tested in media with estradiol 10 nM We also analysed the expression of pS2 gene. For the analysis of pS2 gene expression we employed PCR primers for pS2 and for β-actin as a housekeeping gene using real-time polymerase chain reaction (RT-PCR).Results: Based on cytotoxic activity assay in MCF-7, the active fractions are ethyl acetic fraction and its phases ethyl acetic (EA) 2 and EA 2.2. The most active fraction was EA 2.2 (IC50=27.129 ppm), which exhibited a biphasic effect; at low concentrations, it stimulated the growth, and at high concentrations it showed strong cytotoxic effects. EA2.2 fraction in concentration 20 ppm, also could induce pS2 gene expression in media with and without estrogen.Conclusion: The most active fraction was the ethyl acetate phase and further subfractions. The most active fraction also induced the expression of pS2 gene which was studied by RT-PCR.

scholarly journals Regulation of Chloride Intracellular Channel Protein 1 and Caspase -3 mRNA Expression by Hydroethanolic Extract of Aegle marmelos Fruit Human Breast Cancer Cell Line-MCF-7

2021 ◽  
pp. 586-593
Author(s):  
S. Dhivya ◽  
R. Gayatri Devi ◽  
J. Selvaraj ◽  
A. Jothi Priya
Keyword(s):  
Breast Cancer ◽  
Caspase 3 ◽  
Cancer Cell Line ◽  
Human Breast ◽  
Rt Pcr ◽  
Aegle Marmelos ◽  
Hydroethanolic Extract ◽  
The Given ◽  
Intracellular Channel ◽  
Mcf 7

Introduction: Cancer is the second leading cause of death all over the world where among all types of cancer breast cancer is said to be the leading cancer followed by lung cancer. The aim of this study is to find the regulation of chloride intracellular channel protein 1 and caspase -3 mRNA expression by hydroethanolic extract of Aegle marmelos fruit human breast cancer cell line-MCF-7. Materials and methods: MCF-7 cells were collected from NCCS Pune, India. It is stored in Dubecos Modified Eagle's Medium. The Aegle marmelos fruit was collected from the herbal department and its extract was prepared. The extract of Aegle marmelosis used in treating MCF-7 cells at different dosages in in vitro.  Isolation of total RNA from MCF-7 cells. The cells will be mixed with total RNA isolation reagent, sonicated and RNA will be isolated as per the standard method. c-DNA conversion and real time polymerase chain reaction. The c-DNA will be synthesized using reverse transcription by commercially available (RT-PCR) kit. Two microlitres of c-DNA will be used for amplification of clic-1 and caspase-3 using gene specific primers by commercially available RT-PCR kit (SyBr kit) and comparative CT method will be used to see the expression of genes. Untreated MCF-7 cells were compared with MCF-7 cells treated with various concentrations of the extract (10, 20 and 40ug). The statistical data’s were collected from the SPSS software version 21. Result: The given extract inhibits the proliferation of MCF-7 cells therefore said to have antiproliferative activity. Different doses of extract were tested (200ug-500ug) out of which 400ug of extract were preferred. Conclusion: The given plant extract has anti proliferative properties and hence can be used as a drug to treat breast cancer.

scholarly journals Effects of Two Isolated Compounds from Red Sorghum Bran on p53 and bcl 2 Gene Expression in Human Breast cancer Cell Line (MCF-7)

2021 ◽  
Author(s):  
P.Suganya devi ◽  
M.Saravana kumar ◽  
S.Mohan das
Keyword(s):  
Breast Cancer ◽  
Gene Expression ◽  
Cancer Cell ◽  
Breast Cancer Cell ◽  
Human Breast Cancer ◽  
Cancer Cell Line ◽  
Human Breast Cancer Cell ◽  
Human Breast ◽  
Sorghum Bran ◽  
Mcf 7

Abstract Aims: The aim of our research was to isolate anthocyanin from red sorghum bran and to study its effects on p53 and bcl 2 gene expressions in Human Breast cancer cell line (MCF-7). Main methods: Two compounds were isolated from red sorghum bran by using Sephadex LH-20 column chromatography. The antiproliferative activity of isolated compounds were analysed by MTT assay and the expression studies were performed by RT-PCR analysis.Key findings: Our research focusing on apigenin-5-β-D-glucopyranoside and luteolin-5-β-D-glucopyranoside another compound as a potention anticancer agent have demonstrated that the compounds isolated from red sorghum bran inhibited breast cancer cell lines by up regulation of P53 gene expression and down regulation of Bcl 2 gene expression. Significance: In conclusion, the two compounds isolated from red sorghum bran, were effective in up regulation of P53 and down regulation of Bcl 2 gene expression.

scholarly journals Curcumin inhibits the expression of ornithine decarboxylase and adenosine deaminase genes in MCF-7 human breast cancer cells

2018 ◽  
Vol 70 (4) ◽  
pp. 639-645 ◽  
Author(s):  
Hossein Abbaspour ◽  
Afshar Safipour
Keyword(s):  
Breast Cancer ◽  
Gene Expression ◽  
Breast Cancer Cell Line ◽  
Human Breast Cancer ◽  
Breast Cancer Cells ◽  
Cancer Cell Line ◽  
Malignant Cells ◽  
Human Breast ◽  
Dose Dependent ◽  
Mcf 7

Curcumin is the active ingredient of Curcuma longa, which inhibits the development of malignant cells. Prevention and treatment of cancer by natural compounds, especially curcumin, and understanding the mechanism of action, is an area of interest in cancer research. In this study, we evaluated the effects of curcumin on cell proliferation, ornithine decarboxylase 1 (ODC1) and adenosine deaminase (ADA) gene expression in human breast cancer cell line (MCF-7) as compared to the non-cancer line (MCF-10A). Both cell lines were subjected to increasing doses of curcumin, ranging from 0 to 30 ?g/mL. Cell viability was quantified by the MTT assay. In vitro clonogenic survival assay was performed on MCF-7 cells. Expression of ADA and ODC1 were analyzed by Western blotting and qRT-PCR. Curcumin inhibited the growth of malignant cells in a time- and dose-dependent manner. The calculated IC50 value for MCF-7 cells in 48 h was 12 ?g/mL. Forty-five to 70% decreases in colony formation were observed in MCF-7 cells treated with 30-60 ?g/mL curcumin, respectively. Our data revealed a dose-dependent downregulation of ODC1 and ADA expression and respective enzyme activities by curcumin, which correlated with decreased proliferation in the MCF-7 breast cancer cell line. These data suggest that curcumin represses the proliferation of breast cancer cells through downregulation of ODC1 and ADA gene expression, which might be another mechanism of curcumin-mediated tumor growth inhibition.

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