TOXICITY ANALYSIS OF CRAB SHELL CHITOSAN ARGINYLGLYCYLASPARTIC ACID SCAFFOLD MEMBRANE AND ITS EFFECT ON HUMAN DENTAL PULP CELL VIABILITY

Objective: Crab shell chitosan is a biomaterial used for scaffolding. In Indonesia, Badan Tenaga Nuklir Nasional has made a crab shell chitosan arginylglycylaspartic acid (RGD) scaffold membrane. The purpose of adding RGD was to enhance cell attachment to the scaffold. The objective of this research is to analyze the toxicity of crab shell chitosan RGD scaffold membrane on human dental pulp cells and its effect on their viabilityMethods: Human dental pulp cells were cultured for 5 days in Minimum Essential Medium Alpha (α-MEM) complete containing amphotericin B, penicillin, streptomycin, and fetal bovine serum. Then, the treatment group was exposed to crab shell chitosan RGD scaffold membrane and crab shell chitosan scaffold membrane incubated for 24 h. The toxicity of the crab shell chitosan RGD scaffold membrane was analyzed with a 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay.Result: The result of this research is that crab shell chitosan RGD scaffold membrane did not decrease the percentage of viability of human dental pulp cells.Conclusion: It is concluded that crab shell chitosan RGD scaffold membrane does not have toxic effects on human dental pulp cells.

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TOXICITY ANALYSIS OF RGD-CHITOSAN FROM SHRIMP SHELL SCAFFOLD MEMBRANES TOWARD HUMAN DENTAL PULP CELLS

International Journal of Applied Pharmaceutics ◽

10.22159/ijap.2017.v9s1.07_08 ◽

2017 ◽

Vol 9 ◽

pp. 13

Author(s):

Mauldina Shabrina ◽

Dewi Fatma Suniarti ◽

Lisa R Amir ◽

Erik Idrus

Keyword(s):

Cell Viability ◽

Dental Pulp ◽

Control Group ◽

Dental Pulp Cells ◽

Human Dental Pulp Cells ◽

Membrane Toxicity ◽

Toxicity Analysis ◽

Human Dental Pulp ◽

Pulp Cells ◽

Group 2

Objective: This study aimed to analyze RGD-Chitosan from Shrimp Shells’ Scaffolds’ (RCSSS) and CSSS membrane toxicity toward human dental pulpcells.Methods: Human dental pulp cells were cultured for 5 days and then exposed to RCSSS or CSSS membranes for 24 hrs. Cell viability was determinedusing an MTT assay method.Results: Cell viability of the RCSSS group and CSSS group was higher than the cell viability of the control group. The cell viability of the RCSSSgroup 2 mg (537.39%) was significantly higher than the CSSS group 2 mg (301.74%).Conclusions: RCSSS membranes were not toxic toward human dental pulp cells and showed better effect toward human dental pulp cells comparedto CSSS membranes.

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Cytotoxic Effect of Chitosan Nanoparticles on Normal Human Dental Pulp Cells

Nanoscience and Nanotechnology ◽

10.18063/nn.v3i1.940 ◽

2019 ◽

Vol 3 (1) ◽

Author(s):

Rami Alhomrany ◽

Chang Zhang ◽

Laisheng Chou

Keyword(s):

Cell Viability ◽

Dental Pulp ◽

Cell Attachment ◽

Chitosan Nanoparticles ◽

Dental Pulp Cells ◽

Human Dental Pulp Cells ◽

Attachment Efficiency ◽

Human Dental Pulp ◽

Pulp Cells ◽

Normal Human

Introduction: Recent in vitro studies have shown that chitosan nanoparticles could enhance the antimicrobial activity of several dental materials. However, the biocompatibility of these nanoparticles with normal human cells is still controversial. The aim of this study was to evaluate the potential toxicity of various sizes and concentrations of chitosan nanoparticles cultured with normal human dental pulp cells. Methods: Normal human dental pulp cells were derived from human dental pulp tissues and cultured with (50-67) nm and (318-350) nm chitosan nanoparticles in concentrations: 0.2 mg/mL, 0.5 mg/mL, 1 mg/mL, and 2 mg/mL as study groups, and 0 mg/mL as a control. The cell attachment efficiency for each group was assessed at 16 hours. The proliferation rate and cell viability were evaluated at days 7 and 14. Both, attachment efficiency and proliferation rate were assessed by measuring the optical density of crystal violet stained cells. The cell viability was determined by the activity of the mitochondrial dehydrogenase enzyme. Statistical analysis was performed using One-Way ANOVA and post hoc Tukey test. Results: All concentrations of the (50-67) nm group significantly reduced cell attachment efficiency in comparison with the control (p<0.01) and with the (318-350) nm group (p<0.01). All concentrations of both groups, (50-67) nm and (318-350) nm, significantly reduced cell proliferation and cell viability compared to the control in dose-dependent and size-associated manners. (p<0.01). Conclusion: Chitosan nanoparticles exhibit a cytotoxic effect on normal human dental pulp cells

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Effect of Magnesium on Dentinogenesis of Human Dental Pulp Cells

International Journal of Biomaterials ◽

10.1155/2021/6567455 ◽

2021 ◽

Vol 2021 ◽

pp. 1-12

Author(s):

Rania M. Salem ◽

Chang Zhang ◽

Laisheng Chou

Keyword(s):

Cell Proliferation ◽

Dental Pulp ◽

Cell Attachment ◽

Proliferation Rate ◽

Dental Pulp Cells ◽

Human Dental Pulp Cells ◽

Pulp Capping ◽

Alp Activity ◽

Human Dental Pulp ◽

Pulp Cells

Introducing therapeutic ions into pulp capping materials has been considered a new approach for enhancing regeneration of dental tissues. However, no studies have been reported on its dentinogenic effects on human dental pulp cells (HDPCs). This study was designed to investigate the effects of magnesium (Mg2+) on cell attachment efficiency, proliferation, differentiation, and mineralization of HDPCs. HDPCs were cultured with 0.5 mM, 1 mM, 2 mM, 4 mM, and 8 mM concentrations of supplemental Mg2+ and 0 mM (control). Cell attachment was measured at 4, 8, 12, 16, and 20 hours. Cell proliferation rate was evaluated at 3, 7, 10, 14, and 21 days. Crystal violet staining was used to determine cell attachment and proliferation rate. Alkaline phosphatase (ALP) activity was assessed using the fluorometric assay at 7, 10, and 14 days. Mineralization of cultures was measured by Alizarin red staining. Statistical analysis was done using multiway analysis of variance (multiway ANOVA) with Wilks’ lambda test. Higher cell attachment was shown with 0.5 mM and 1 mM at 16 hours compared to control ( P < 0.0001 ). Cells with 0.5 mM and 1 mM supplemental Mg2+ showed significantly higher proliferation rates than control at 7, 10, 14, and 21 days ( P < 0.0001 ). However, cell proliferation rates decreased significantly with 4 mM and 8 mM supplemental Mg2+ at 14 and 21 days ( P < 0.0001 ). Significantly higher levels of ALP activity and mineralization were observed in 0.5 mM, 1 mM, and 2 mM supplemental Mg2+ at 10 and 14 days ( P < 0.0001 ). However, 8 mM supplemental Mg2+ showed lower ALP activity compared to control at 14 days ( P < 0.0001 ), while 4 mM and 8 mM supplemental Mg2+showed less mineralization compared to control ( P < 0.0001 ). The study indicated that the optimal (0.5–2 mM) supplemental Mg2+ concentrations significantly upregulated HDPCs by enhancing cell attachment, proliferation rate, ALP activity, and mineralization. Magnesium-containing biomaterials could be considered for a future novel dental pulp-capping additive in regenerative endodontics.

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Comparative Surface Morphology, Chemical Composition, and Cytocompatibility of Bio-C Repair, Biodentine, and ProRoot MTA on hDPCs

Materials ◽

10.3390/ma13092189 ◽

2020 ◽

Vol 13 (9) ◽

pp. 2189 ◽

Cited By ~ 1

Author(s):

James Ghilotti ◽

José Luis Sanz ◽

Sergio López-García ◽

Julia Guerrero-Gironés ◽

María P. Pecci-Lloret ◽

...

Keyword(s):

Chemical Composition ◽

Dental Pulp ◽

Cell Attachment ◽

Biological Effects ◽

Control Group ◽

Essential Property ◽

Dental Pulp Cells ◽

Human Dental Pulp Cells ◽

Human Dental Pulp ◽

Pulp Cells

Biocompatibility is an essential property for any vital pulp material that may interact with the dental pulp tissues. Accordingly, this study aimed to compare the chemical composition and ultrastructural morphology of Biodentine (Septodont, Saint Maur-des-Fosses, France), ProRoot MTA (Dentsply Tulsa Dental Specialties, Johnson City, TN, USA), and Bio-C Repair (Angelus, Londrina, PR, Brazil), as well as their biological effects on human dental pulp cells. Chemical element characterization of the materials was undertaken using scanning electron microscopy and energy dispersive X-ray analysis (SEM-EDX). The cytotoxicity was assessed by analyzing the cell viability (MTT assay), cell morphology (immunofluorescence assay), and cell attachment (flow cytometry assay). The results were statistically analyzed using ANOVA and Tukey’s test (p < 0.05). EDX revealed that ProRoot MTA and Biodentine were mostly composed of calcium, carbon, and oxygen (among others), whereas Bio-C Repair evidenced a low concentration of calcium and the highest concentration of zirconium. SEM showed adequate attachment of human dental pulp cells (hDPCS) to vital pulp materials and cytoskeletal alterations were not observed in the presence of material eluates. Remarkably, the undiluted Biodentine group showed higher viability than the control group cells (without eluates) at 24 h, 48 h, and 72 h (p < 0.001). Based on the evidence derived from an in vitro cellular study, it was concluded that Bio-C Repair showed excellent cytocompatibility that was similar to Biodentine and ProRoot MTA.

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Integrin expression in human dental pulp cells and their role in cell attachment on extracellular matrix proteins

Journal of Endodontics ◽

10.1016/s0099-2399(98)80145-3 ◽

1998 ◽

Vol 24 (10) ◽

pp. 641-644 ◽

Cited By ~ 10

Author(s):

Qiang Zhu ◽

Kamran E. Safavi ◽

Larz S.W. Spångberg

Keyword(s):

Extracellular Matrix ◽

Dental Pulp ◽

Cell Attachment ◽

Extracellular Matrix Proteins ◽

Matrix Proteins ◽

Integrin Expression ◽

Dental Pulp Cells ◽

Human Dental Pulp Cells ◽

Human Dental Pulp ◽

Pulp Cells

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The Preparation and Characterization of Chitosan-Based Hydrogels Cross-Linked by Glyoxal

Materials ◽

10.3390/ma14092449 ◽

2021 ◽

Vol 14 (9) ◽

pp. 2449

Author(s):

Beata Kaczmarek-Szczepańska ◽

Olha Mazur ◽

Marta Michalska-Sionkowska ◽

Krzysztof Łukowicz ◽

Anna Maria Osyczka

Keyword(s):

Thermal Stability ◽

Tannic Acid ◽

Dental Pulp ◽

Blood Compatibility ◽

Dental Pulp Cells ◽

Preliminary Assessment ◽

Human Dental Pulp Cells ◽

Human Dental Pulp ◽

Pulp Cells

In this study, hydrogels based on chitosan cross-linked by glyoxal have been investigated for potential medical applications. Hydrogels were loaded with tannic acid at different concentrations. The thermal stability and the polyphenol-releasing rate were determined. For a preliminary assessment of the clinical usefulness of the hydrogels, they were examined for blood compatibility and in the culture of human dental pulp cells (hDPC). The results showed that after immersion in a polyphenol solution, chitosan/glyoxal hydrogels remain nonhemolytic for erythrocytes, and we also did not observe the cytotoxic effect of hydrogels immersed in tannic acid (TA) solutions with different concentration. Tannic acid was successfully released from hydrogels, and its addition improved material thermal stability. Thus, the current findings open the possibility to consider such hydrogels in clinics.

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