An In-Vitro Study Comparing the Effects of Citrus Lemon Extract on Porphyromonas Gingivalis, Aggregatibacter Actinomycetemcomitans, and Prevotella Intermedia

2021 ◽  
Vol 9 (11) ◽  
pp. 1546-1550
Author(s):  
Dr. Shivani Vansia
Keyword(s):  
Porphyromonas Gingivalis ◽  
Periodontal Diseases ◽  
In Vitro Study ◽  
Extended Period ◽  
Culture Method ◽  
Aggregatibacter Actinomycetemcomitans ◽  
Prevotella Intermedia ◽  
Periodontal Pathogens ◽  
Zone Of Inhibition

Abstract: Plant extracts and phytochemicals having antimicrobial characteristics could be immensely beneficial in medicinal treatments. The present study deals with the effect of Citrus lemon extract over Periodontal pathogens which are primary etiologic factors for periodontal diseases. The current study took into account periodontal pathogens such as Prevotella Intermedia, Aggregatibacter Actinomycetemcomitans, and Porphyromonas Gingivalis. Chlorhexidine is a gold standard antimicrobial agent with a wide antibacterial activity that is commonly used for chemical plaque management. When used for an extended period, however, chlorhexidine is known to stain. As a result, the alternatives are to be explored such as herbal-based agents that can be used regularly. Using the microdilution process and the culture method, the antibacterial effect of citrus lemon extract against periodontal pathogens was assessed using the minimum inhibitory concentration (MIC), minimum bactericidal concentration (MBC), and zone of inhibition (ZOI). According to the findings of this study, citrus lemon extract can be used as a natural supplement for treatment purposes. Keywords: Antimicrobial, Citrus lemon Extract, Chlorhexidine, Periodontal Pathogens.

scholarly journals The Antimicrobial Activity of the Three Commercially Available Intense Sweeteners against Common Periodontal Pathogens: An in vitro Study

2012 ◽  
Vol 13 (6) ◽  
pp. 749-752 ◽  
Author(s):  
Ravi B Patil ◽  
Tejavathi Nagaraj ◽  
Vinit B Patel
Keyword(s):  
Antimicrobial Activity ◽  
Dental Caries ◽  
Porphyromonas Gingivalis ◽  
Periodontal Diseases ◽  
In Vitro Study ◽  
Aggregatibacter Actinomycetemcomitans ◽  
Vitro Study ◽  
Periodontal Pathogens ◽  
Zone Of Inhibition

ABSTRACT Aim To evaluate the in vitro antimicrobial activity of three commercially available intense sweeteners against two common periodontal pathogens Porphyromonas gingivalis and Aggregatibacter actinomycetemcomitans. Materials and methods Three commercially available intense sweeteners namely saccharin, aspartame and sucralose were obtained and powdered. Necessary concentrations of the sweeteners were prepared by mixing them with an inert solvent. The antimicrobial efficacy was assessed using agar well diffusion technique. Statistical analysis was done using one-way ANOVA followed by Tukey's post hoc test. p-value < 0.05 was considered statistically significant. Results All the three sweeteners showed significant antimicrobial activity against the periodontal pathogens tested. Sucralose containing sucralose showed maximum zone of inhibition, against Aggregatibacter actinomycetemcomitans. Saccharin and aspartame containing saccharin and aspartame respectively, showed maximum zone of inhibition, against Porphyromonas gingivalis. Conclusion All the sweeteners used in this study have demonstrated significant antimicrobial activity. Therefore, these sweeteners could be recommended as an ideal alternative to sucrose. Clinical significance Dental caries and periodontal diseases are ubiquitous diseases of mankind caused by microorganisms. Dental caries is caused by sucrose. By altering the source like intense sweetener we can combat caries as well as with its antimicrobial properties against periodontopathic bacteria, we can reduce prevalence of periodontal diseases. How to cite this article Prashant GM, Patil RB, Nagaraj T, Patel VB. The Antimicrobial Activity of the Three Commercially Available Intense Sweeteners against Common Periodontal Pathogens: An in vitro Study. J Contemp Dent Pract 2012; 13(6):749-752.

scholarly journals PHOTOTOXIC EFFECT OF VISIBLE BLUE LIGHT ON AGGREGATIBACTER ACTINOMYCETEMCOMITANS AND PORPHYROMONAS GINGIVALIS ISOLATED FROM PATIENTS WITH CHRONIC PERIODENTITIS: AN IN-VITRO EXPERIMENTAL STUDY

2019 ◽  
Author(s):  
Mohsen Ali Al-Hamzi ◽  
Ayman Ahmed Ahmed Al-jorany ◽  
Hassan A. Al-Shamahy ◽  
Amani Abdulhakeem Al-Sharani
Keyword(s):  
Blue Light ◽  
Porphyromonas Gingivalis ◽  
Light Exposure ◽  
Periodontal Diseases ◽  
Aggregatibacter Actinomycetemcomitans ◽  
Common Disease ◽  
Periodontal Pathogens ◽  
Bacterial Killing ◽  
Local Risk

Chronic periodentitis is a quite common disease in adult patients characterized by pocket formation and/or recession while progressive loss of periodontal attachment occurs slowly to moderately local risk factors, e.g. bacterial plaque. Wide array of microorganisms have been associated with periodontal disease, out of which Aggregatibacter actinomycetemcomitans (Aa) and Porphyromonas gingivalis (Pg) have been predominantly associated with periodontal diseases. The objectives of this study were to determine phototoxic effect of visible blue light on Aggregatibacter actinomycetemcomitans and Porphyromonas gingivalis clinical isolates from chronic periodentitis patients, and to study their antibiotic sensitivity against selected antibiotics. The test was carried out on 15 strains of Aggregatibacter actinomycetemcomitans and 15 strains of Porphyromonas gingivalis isolated from pockets of chronic periodentitis patients aged between 30-50 years old with pocket depths of 5-6 mm. The bacteria cultured, isolated, and identified by standard bacteriological methods, then subjected to visible blue light at different periods of time exposures. After light exposure, the bacterial killing rates were calculated from colony forming unit (CFU) counts after 48hours of anaerobic incubation. There was a decrease in CFU for both microorganisms as we proceeded from zero, 20, 40 and 60 seconds of blue light exposure. In conclusions, there was a phototoxic effect for the visible blue light emitted from the light curing device against the anaerobic periodontal pathogens and blue light exposure is effective in reducing periodontal pathogens. It is recommended that an adjunctive exogenous photosensitizer be used and that pathogens be exposed to visible light for clinical antimicrobial periodontal therapy.

In vitro Resistance Testing of Porphyromonas gingivalis, Prevotella intermedia, and Tannerella forsythia to Triclosan

2016 ◽  
Vol 17 (4) ◽  
pp. 282-285 ◽  
Author(s):  
Deema J Farsi ◽  
Anne Tanner
Keyword(s):  
Escherichia Coli ◽  
Porphyromonas Gingivalis ◽  
Vitamin K ◽  
Prolonged Exposure ◽  
Resistance Testing ◽  
Prevotella Intermedia ◽  
Periodontal Pathogens ◽  
Tannerella Forsythia ◽  
Prolonged Incubation

ABSTRACT Aim To determine the sensitivity of Porphyromonas gingivalis, Prevotella intermedia, and Tannerella forsythia to triclosan, and determine if these bacteria develop resistance to triclosan upon prolonged exposure. Materials and methods Susceptibility to triclosan was tested against three periodontal pathogens P. gingivalis, P. intermedia, and T. forsythia. Escherichia coli strains sensitive and resistant to triclosan were used as biological controls to confirm the efficacy of triclosan in the assays. Agar plates were prepared locally with vitamin K and hemin-supplemented medium. Results Porphyromonas gingivalis and P. intermedia did not grow on plates containing ≥2 μg/ml triclosan, while T. forsythia did not grow on ≥1.66 μg/ml. Colonies of P. intermedia resistant to triclosan developed after prolonged incubation at 2 μg/ml, but this resistance disappeared during subculture in the absence of triclosan. Conclusion No significant resistance to triclosan was detected for these species. Clinical significance Dental products containing triclosan can be beneficial in controlling periodontal disease. How to cite this article Farsi D, Tanner A. In vitro Resistance Testing of Porphyromonas gingivalis, Prevotella intermedia, and Tannerella forsythia to Triclosan. J Contemp Dent Pract 2016;17(4):282-285.

scholarly journals Inhibitory Effects of Lactoferrin on Growth and Biofilm Formation of Porphyromonas gingivalis and Prevotella intermedia

2009 ◽  
Vol 53 (8) ◽  
pp. 3308-3316 ◽  
Author(s):  
Hiroyuki Wakabayashi ◽  
Koji Yamauchi ◽  
Tetsuo Kobayashi ◽  
Tomoko Yaeshima ◽  
Keiji Iwatsuki ◽  
...  
Keyword(s):  
Porphyromonas Gingivalis ◽  
Biofilm Formation ◽  
Periodontal Diseases ◽  
Antimicrobial Protein ◽  
Antibiofilm Activity ◽  
Prevotella Intermedia ◽  
Periodontopathic Bacteria ◽  
Oral Pathogens ◽  
In Vitro Effects

ABSTRACT Lactoferrin (LF) is an iron-binding antimicrobial protein present in saliva and gingival crevicular fluids, and it is possibly associated with host defense against oral pathogens, including periodontopathic bacteria. In the present study, we evaluated the in vitro effects of LF-related agents on the growth and biofilm formation of two periodontopathic bacteria, Porphyromonas gingivalis and Prevotella intermedia, which reside as biofilms in the subgingival plaque. The planktonic growth of P. gingivalis and P. intermedia was suppressed for up to 5 h by incubation with ≥130 μg/ml of human LF (hLF), iron-free and iron-saturated bovine LF (apo-bLF and holo-bLF, respectively), and ≥6 μg/ml of bLF-derived antimicrobial peptide lactoferricin B (LFcin B); but those effects were weak after 8 h. The biofilm formation of P. gingivalis and P. intermedia over 24 h was effectively inhibited by lower concentrations (≥8 μg/ml) of various iron-bound forms (the apo, native, and holo forms) of bLF and hLF but not LFcin B. A preformed biofilm of P. gingivalis and P. intermedia was also reduced by incubation with various iron-bound bLFs, hLF, and LFcin B for 5 h. In an examination of the effectiveness of native bLF when it was used in combination with four antibiotics, it was found that treatment with ciprofloxacin, clarithromycin, and minocycline in combination with native bLF for 24 h reduced the amount of a preformed biofilm of P. gingivalis compared with the level of reduction achieved with each agent alone. These results demonstrate the antibiofilm activity of LF with lower iron dependency against P. gingivalis and P. intermedia and the potential usefulness of LF for the prevention and treatment of periodontal diseases and as adjunct therapy for periodontal diseases.

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