scholarly journals Medium-term and long-term in vitro conservation and safe international exchange of yam (Dioscorea spp.) germplasm

1998 ◽  
Vol 1 (2) ◽  
pp. 103-117 ◽  
Author(s):  
Bernard Malaurie ◽  
Marie-France Trouslot ◽  
Julien Berthaud ◽  
Mustapha Bousalem ◽  
Agnes Pinel ◽  
...  
Keyword(s):  
In Vitro Conservation ◽  
International Exchange ◽  
Medium Term

Medium-term in vitro conservation of virus-free parthenocarpic tomato plants

2018 ◽  
Vol 54 (4) ◽  
pp. 392-398 ◽  
Author(s):  
Sota Koeda ◽  
Shotaro Matsumoto ◽  
Yuki Matsumoto ◽  
Rihito Takisawa ◽  
Koji Nishikawa ◽  
...  
Keyword(s):  
In Vitro Conservation ◽  
Medium Term ◽  
Tomato Plants

scholarly journals Long-term conservation of potato genetic resources: Methods and status of conservation

2019 ◽  
pp. 717-725
Author(s):  
Jane Muthoni ◽  
Hussein Shimelis ◽  
Rob Melis
Keyword(s):  
Genetic Resources ◽  
Slow Growth ◽  
Plant Genetic Resources ◽  
Ex Situ ◽  
Growth Media ◽  
Medium Term ◽  
Natural Habitats

Plant genetic resources (PGRs) play an important role in agriculture, environment protection, cultural property and trade; they need to be conserved. There are two fundamental approaches for the conservation of PGRs: in situ and ex situ. In situ conservation is the conservation of ecosystems and natural habitats and the maintenance and recovery of viable populations of species in their natural surroundings. Ex situ preservation is the storage of seeds or plant materials under artificial conditions to maintain their long term viability and availability for use. Genebanks employ seed storage, field collections of living plants and in vitro storage (tissue culture or cryopreservation) for ex situ preservation of PGR. Storage of orthodox seeds, which are tolerant to low moisture content and low temperatures at appropriate temperature and humidity, is the most convenient ex situ conservation method. Plants that produce recalcitrant seeds or non-viable seeds are conserved in field genebanks as well as in-vitro in slow growth media for short-to-medium term and cryopreservation in liquid nitrogen at -1960C for long-term periods. Cryopreservation is very expensive and needs trained personnel; this could explain why this method is rarely used for conservation of plant genetic resources in most developing countries. Potato tubers are bulky and highly perishable; the crop is generally conserved as clones either in field genebanks (with annual replanting), in-vitro conservation in slow growth media for short-to-medium term and cryopreservation for long term. Field genebanks are expensive to maintain and the crop is exposed to many dangers; hence, cryopreservation is the only feasible method for long term conservation. However, given the high cost of cryopreservation, long-term conservation of potato genetic resources is poorly developed in most resource-poor countries leading to high rates of genetic erosion. This paper looks into the various methods that that can be applied to conserve potato genetic resources and the status of conservation of potatoes in major genebanks and some countries.

MEDIUM- AND LONG- TERM IN VITRO CONSERVATION OF OLIVE GERMPLASM (OLEA EUROPAEA L.)

2002 ◽  
pp. 109-112 ◽  
Author(s):  
M. Lambardi ◽  
C. Benelli ◽  
A. De Carlo ◽  
A. Fabbri ◽  
S. Grassi ◽  
...  
Keyword(s):  
Olea Europaea ◽  
In Vitro Conservation ◽  
Olea Europaea L ◽  
Olea Europaéa

A Novel Strategy for in vitro Conservation of Aloe vera L. through Long Term Shoot Culture

2010 ◽  
Vol 9 (3) ◽  
pp. 326-331 ◽  
Author(s):  
S. Gantait ◽  
N. Mandal ◽  
S. Bhattachar ◽  
P.K. Das
Keyword(s):  
Aloe Vera ◽  
Shoot Culture ◽  
In Vitro Conservation ◽  
Novel Strategy

Medium-term in vitro conservation of Castanea spp. hybrid clones

2021 ◽  
Vol 34 (1) ◽  
pp. 127-137
Author(s):  
Filomena Gomes ◽  
Marta Clemente ◽  
Patricia Figueiredo ◽  
Rita Lourenço Costa
Keyword(s):  
In Vitro Conservation ◽  
Medium Term ◽  
Hybrid Clones

scholarly journals Medium Term Conservation of Several Carnation Accessions Via in Vitro Culture

2012 ◽  
Vol 13 (2) ◽  
pp. 174
Author(s):  
Kurniawan Budiarto ◽  
Budi Marwoto
Keyword(s):  
Low Temperature ◽  
Significant Variation ◽  
Root Formation ◽  
In Vitro Conservation ◽  
Medium Term ◽  
Low Temperature Storage ◽  
Two Factors ◽  
Temperature Storage

Sufficient genetic diversity is important in carnation breeding program. In vivo conservation of carnation germplasmis considered inefficient due to some technical and economical aspects. In vitro conservation was then, expectedto overcome the limitation of in vivo method. The research was conducted to find out the proper media for medium-term in vitro conservation of several carnation accessions in low temperature storage. A complete factorialexperiment with 25 replications was designed to accomplish the combination of two factors. The first factor wassix commercial carnation cultivars, namely Pink Maladi, Orange Triumph, Opera, Tundra, Yellow Liberty and PradoReffit. The second factor was the conservation media i.e. 1⁄2MS + DMSO 3% and 1⁄2MS + 3% DMSO + 3% sucrose andcontrol (MS 0+3% sucrose). The results showed that in vitro conservation of carnation in low temperature weresuccessfully conducted using 1⁄2MS+3% DMSO and 1⁄2MS+3% DMSO+3% sucrose without significant variation in allaccessions tested up to 10 and 12 months respectively. The increase of death plantlets, however, was detected onthe media of 1⁄2MS+3% DMSO after 6 months storage with significant decrease in viability hereafter. The existenceof sucrose in DMSO media induced root formation and plantlet resistance to low temperature storage.

scholarly journals Recurrent somatic embryogenesis in long-term cultures of Gentiana lutea L. as a source for synthetic seed production for medium-term preservation

2012 ◽  
Vol 64 (2) ◽  
pp. 809-817 ◽  
Author(s):  
Irina Holobiuc ◽  
R. Catana
Keyword(s):  
Somatic Embryogenesis ◽  
Seed Production ◽  
Somatic Embryos ◽  
Plant Material ◽  
Synthetic Seed ◽  
Sugar Alcohols ◽  
Medium Term ◽  
Gentiana Lutea

Our aim was to establish an efficient and reproducible system for producing synthetic seeds from recurrent somatic embryogenesis in long-term cultures of Gentiana lutea L. This species is a vulnerable medicinal plant, protected both at the national and international levels, and is included in different Red Lists and Books. In vitro culture, as an alternative to classical methods of preservation, allows for the cyclic multiplication of plant material and short-, medium- and long-term preservation of tissue collections. Biotechnological approaches allow for maintenance of the plant material in a confined space and protection against biotic and abiotic factors. Somatic embryogenesis (SE) is the most efficient way to regenerate plants, ensuring material for preservation and fundamental research. In our experiment, recurrent somatic embryogenesis was developed in long-term cultures in the presence of sugar alcohols (mannitol, sorbitol) and in the absence of growth factors. This process proceeded at a high rate, with adventive somatic embryos being generated in a continuous process, followed by maturation, germination and development into plants. To follow the somatic embryogenesis process, histological samples were made. We used these embryogenic cultures for synthetic seed production and medium-term conservation. The viability of somatic embryos after moderate osmotic stress treatment was tested using TTC. Our methodology relied on the induction of somatic embryogenesis in the presence of auxins in the first cycle of in vitro cultures, long-term high embryogenic culture maintenance in the presence of sugar alcohols and synthetic seed production.

scholarly journals Teknik Enkapsulasi Sederhana untuk Konservasi In vitro Jangka Menengah Tanaman Nenas (Ananas comosus) [Simple Encapsulation Technique for Medium Term Pineapple (Ananas comosus) In vitro Conservation]

2019 ◽  
Vol 29 (1) ◽  
pp. 1
Author(s):  
Riry Prihatini ◽  
Sri Hadiati
Keyword(s):  
Shelf Life ◽  
Sodium Alginate ◽  
Incubation Temperature ◽  
Genetic Material ◽  
Synthetic Seeds ◽  
In Vitro Conservation ◽  
Ananas Comosus ◽  
Medium Term ◽  
Tropical Fruit

<p>Konservasi in vitro tanaman nenas dilakukan untuk penyimpanan materi genetik sebelum dimanfaatkan. Penelitian ini dilaksanakan untuk mengembangkan teknik enkapsulasi yang dapat memperpanjang daya simpan benih sintetik nenas melalui perlakuan konsentrasi natrium alginat, suhu, dan media penyimpanan. Penelitian dilakukan di Laboratorium Kultur Jaringan, Balai Penelitian Tanaman Buah Tropika, mulai Januari hingga Desember 2017. Bahan yang digunakan adalah plantlet nenas aksesi 5X18(10). Penelitian dibagi menjadi dua subkegiatan. Metode yang digunakan pada subkegiatan pertama yaitu tunas mikro nenas dienkapulasi dengan metode tetes menggunakan natrium alginat 3% dan 4% serta penyimpanan dalam akuades steril dan tanpa media selama 30, 60, 120, dan 240 hari pada suhu 25oC. Penggunaan 4% natrium alginat dan media akuades steril dapat memperpanjang masa simpan benih sintetik nenas hingga 240 hari dengan daya regenerasi benih 100%. Pada subkegiatan kedua, perlakuan terbaik pada subkegiatan pertama dilanjutkan dengan perlakuan suhu penyimpanan 4oC. Benih sintetik nenas pada suhu penyimpanan tersebut hanya mampu bertahan hingga 60 hari, selebihnya tunas dalam benih menghitam dan tidak dapat ditumbuhkan kembali. Metode enkapsulasi untuk penyimpanan materi genetik yang dikembangkan dalam penelitian ini lebih sederhana dan efisien serta dapat diaplikasikan pada kegiatan konservasi in vitro jangka menengah tanaman nenas.</p><p><strong>Keywords</strong></p><p>Enkapsulasi; Konservasi; In vitro;  Tanaman nenas</p><p><strong>Abstract</strong></p><p>In vitro conservation of pineapple was conducted as preservation of genetic material before it was further utilized. This research was conducted to obtain encapsulation technique which expanded synthetic seeds shelf life by modifying concentration of sodium alginate, incubation media, and temperature. The research was conducted on Tissue Culture Laboratory of Indonesian Tropical Fruit Research Institute on January to December 2017. The materials which were used included pineapple micro shoots accessions 5X18(10). The research was divided into subactivities. The method which was applied on the first subactivity included encapsulation of pineapple micro shoots using drop method with sodium alginate 3% and 4%,incubation media sterile aquades and without media for 30, 60, 120, and 240 days on 25oC temperature.The use of 4% sodium alginate and sterile aquades incubation media prolonged the pineapple shelf life up to 240 days with 100% regeneration capability. On the second subactivity, the best treatment on the first activity was combined with 4oC incubation temperature. The pineapple synthetic seeds on this incubation temperature only survive up to 60 days, became blackening, and could not be regrowth. Encapsulation method which was developed on this study was simpler, more efficient, and able to be applied for medium term pineapple in vitro conservation.</p>

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