scholarly journals Medium Term Conservation of Several Carnation Accessions Via in Vitro Culture

2012 ◽  
Vol 13 (2) ◽  
pp. 174
Author(s):  
Kurniawan Budiarto ◽  
Budi Marwoto
Keyword(s):  
Low Temperature ◽  
Significant Variation ◽  
Root Formation ◽  
In Vitro Conservation ◽  
Medium Term ◽  
Low Temperature Storage ◽  
Two Factors ◽  
Temperature Storage

Sufficient genetic diversity is important in carnation breeding program. In vivo conservation of carnation germplasmis considered inefficient due to some technical and economical aspects. In vitro conservation was then, expectedto overcome the limitation of in vivo method. The research was conducted to find out the proper media for medium-term in vitro conservation of several carnation accessions in low temperature storage. A complete factorialexperiment with 25 replications was designed to accomplish the combination of two factors. The first factor wassix commercial carnation cultivars, namely Pink Maladi, Orange Triumph, Opera, Tundra, Yellow Liberty and PradoReffit. The second factor was the conservation media i.e. 1⁄2MS + DMSO 3% and 1⁄2MS + 3% DMSO + 3% sucrose andcontrol (MS 0+3% sucrose). The results showed that in vitro conservation of carnation in low temperature weresuccessfully conducted using 1⁄2MS+3% DMSO and 1⁄2MS+3% DMSO+3% sucrose without significant variation in allaccessions tested up to 10 and 12 months respectively. The increase of death plantlets, however, was detected onthe media of 1⁄2MS+3% DMSO after 6 months storage with significant decrease in viability hereafter. The existenceof sucrose in DMSO media induced root formation and plantlet resistance to low temperature storage.

scholarly journals 519 Apoplastic Levels of Pectins and Free Galactose in Ripening and Chill-injured Tomato Fruit

HortScience ◽  
2000 ◽  
Vol 35 (3) ◽  
pp. 484D-484
Author(s):  
Domingos P. F. Almeida ◽  
Donald J. Huber
Keyword(s):  
Low Temperature ◽  
Tomato Fruit ◽  
Fold Increase ◽  
Wild Type ◽  
Low Temperature Storage ◽  
The Status ◽  
Apoplastic Fluid ◽  
Temperature Storage

Pectin solubility in ripening tomato fruit is typically studied in vitro, employing isolated cell walls; however, it is unknown whether in vitro studies address the actual changes in the status of pectins in the fruit in situ. In vivo pectin solubilization was examined in a pressure-extracted apoplastic fluid obtained from ripening and chill-injured tomato fruit with down-regulated polygalacturonase (PG) activity and untransformed wild-type. Pectin levels in apoplastic fluid increased 3-fold during ripening and were not affected by PG levels. In contrast, PG strongly affected pectin levels in bulk, enzymically active pericarp fluid. There was a 14-fold increase in bulk pectin levels during ripening of PG-antisense fruit and a 36-fold increase in wild-type fruit. Pectin levels in the apoplastic fluid of fruit stored at 5 °C for 14 days were 40% lower than that of freshly harvested mature-green fruit, but increased significantly upon transfer of fruit to 15 °C. Monomeric galactose in the apoplastic fluid increased from 41 mg·mL–1 at the mature-green stage to 67 mg·mL–1 in ripe fruit. Bulk levels of galactose were 3- to 4-fold higher than apoplastic levels. After low-temperature storage galactose levels were 50% and 20% lower than in freshly harvested fruit for the bulk and apoplastic fluids, respectively. These results indicate that in vivo pectin solubilization is restricted and largely independent of PG. Low-temperature storage reduces in vivo pectin solubilization, an effect that is reversed upon transfer of fruit to higher temperature following cold storage.

scholarly journals Low temperature storage and in vitro germination of cherimoya (Annona cherimola Mill.) pollen

2006 ◽  
Vol 108 (1) ◽  
pp. 91-94 ◽  
Author(s):  
J. Lora ◽  
M.A. Pérez de Oteyza ◽  
P. Fuentetaja ◽  
J.I. Hormaza
Keyword(s):  
Low Temperature ◽  
In Vitro Germination ◽  
Low Temperature Storage ◽  
Annona Cherimola ◽  
Temperature Storage

scholarly journals Hypothermic and Low-Temperature Storage of Garlic (Allium sativum L.) for in Vitro Collections

2017 ◽  
Vol 27 (2) ◽  
pp. 110-120
Author(s):  
Tetyana V. Ivchenko ◽  
◽  
Tatyana I. Vitsenya ◽  
Nadiya A. Shevchenko ◽  
Natalia O. Bashtan ◽  
...  
Keyword(s):  
Low Temperature ◽  
Allium Sativum ◽  
Low Temperature Storage ◽  
Temperature Storage

Effect of carbohydrates on in vitro low-temperature storage of shoot cultures of apricot

2010 ◽  
Vol 126 (4) ◽  
pp. 434-440 ◽  
Author(s):  
Grazia Marino ◽  
Paola Negri ◽  
Antonio Cellini ◽  
Andrea Masia
Keyword(s):  
Low Temperature ◽  
Shoot Cultures ◽  
Low Temperature Storage ◽  
Temperature Storage

scholarly journals Responses of Broccoli Seedlings to Light Quality during Low-temperature Storage in Vitro: II. Sugar Content and Photosynthetic Efficiency

HortScience ◽  
1998 ◽  
Vol 33 (7) ◽  
pp. 1258-1261 ◽  
Author(s):  
Sandra B. Wilson ◽  
Keiko Iwabuchi ◽  
Nihal C. Rajapakse ◽  
Roy E. Young
Keyword(s):  
Low Temperature ◽  
Sugar Content ◽  
Soluble Sugar ◽  
Soluble Sugars ◽  
Photon Flux ◽  
Media Composition ◽  
Low Temperature Storage ◽  
Photosynthetic Ability ◽  
Temperature Storage

Broccoli (Brassica oleracea L. Botrytis group `Green Duke') seeds were cultured photoautotrophically (without sugar) or photomixotrophically (with sugar) in vitro for 3 weeks at 23 °C and150 μmol·m-2·s-1 photosynthetic photon flux (PPF). In vitro seedlings were stored for 0, 4, 8, or 12 weeks at 5 °C in darkness or under 5 μmol·m-2·s-1 of white (400–800 nm), blue (400–500 nm), or red (600–700 nm) light. Photosynthetic ability and soluble sugar contents were determined after removal from storage. Photomixotrophic seedlings contained approximately five times more soluble sugars than did photoautotrophic seedlings. Dark storage reduced soluble sugars in both photoautotrophic and photomixotrophic plants, but photosynthetic ability was maintained for up to 8 weeks in the latter whereas it decreased in the former. Illumination in storage increased leaf soluble sgars in both photoautotrophic and photomixotrophic seedlings. Soluble sugars in stems decreased during storage regardless of illumination, but remained higher in illuminated seedlings. Red light was more effective in increasing or maintaining leaf and stem soluble sugars than was white or blue light. Regardless of media composition or illumination, storage for more tan 8 weeks resulted in dramatic losses in quality and recovery, as well as photosynthetic ability. Seedlings stored for 12 weeks comletely lost their photosynthetic ability regardless of media composition or illumination. The results suggest that carbohydrate, supplied in the media or through illumination, is essential for maintenance of photosynthetic ability during low-temperature storage for up to 4 or 8 weeks.

scholarly journals Callus induction in papaya (Carica papaya L.) and synseed production for low temperature storage and cryopreservation

2014 ◽  
Vol 26 (2) ◽  
pp. 155-162 ◽  
Author(s):  
Jaime A. Teixeira da Silva
Keyword(s):  
Low Temperature ◽  
Callus Induction ◽  
Carica Papaya ◽  
Alginate Beads ◽  
Induction Medium ◽  
Zygotic Embryos ◽  
Ms Medium ◽  
Low Temperature Storage ◽  
Temperature Storage

ABSTRACT The mid- to long-term preservation of papaya (Carica papaya L.) would allow for the safeguarding of important germplasm. In this study, soft friable callus (SFC) and hard callus (HC) were induced from the first two true leaves of 10-day-old seedlings containing a midrib derived from the germinated seed of two cultivars (‘Rainbow’ and ‘Sunrise Solo’). Following germination on a Murashige and Skoog (MS) medium that contained 3% sucrose and was free of plant growth regulators (PGRs), sections of the first true leaves from 10-day-old seedlings were exposed to seven published callus or somatic embryogenesis protocols for zygotic embryos, leaves or hypocotyls. Optimal SFC and HC induction was carried out on a half-strength MS medium following the Fitch (1993) or the Ascêncio-Cabral et al. (2008) protocol, respectively. SFC formed shoots that could then convert to plants when transferred to a full-strength MS medium devoid of PGRs. Plantlets 10-cm tall were acclimatised in two steps: first by in vitro acclimatisation in aerated vessels, the Vitron, under CO2-enriched (3000 ppm CO2), then by the transfer of individually rooted plantlets in Rockwool® blocks to a substrate of soil: pine bark : perlite (1:1:1, v/v/v). SFC and HC were then encapsulated in alginate beads, which were exposed to low temperature storage (LTS) at 10°C and 15°C, and also cryopreserved for 30 days. All encapsulated alginate beads that contained SFC, HC or leaf tissue that had been stored under LTS or cryopreserved were able to regenerate callus when placed on an optimal callus induction medium. Plants derived from the control, LTS and cryopreservation protocols, either from SFC or HC, were successfully acclimatised.

scholarly journals Low-temperature Storage for Quality Preservation and Growth Suppression of Broccoli Plantlets Cultured in Vitro

HortScience ◽  
1994 ◽  
Vol 29 (10) ◽  
pp. 1191-1194 ◽  
Author(s):  
Chieri Kubota ◽  
Toyoki Kozai
Keyword(s):  
Low Temperature ◽  
Dry Weight ◽  
Photon Flux ◽  
Photosynthetic Photon Flux ◽  
Fluorescence Kinetics ◽  
Low Temperature Storage ◽  
Fluorescent Lamps ◽  
Chlorophyll Concentrations ◽  
Temperature Storage

Broccoli (Brassica oleracea L. Botrytis Group `Ryokurei') plantlets, cultured photoautotrophically (without sugar in the medium) in vitro for 3 weeks at 23C and 160 μmol·m–2·s–1 photosynthetic photon flux (PPF), were stored for 6 weeks at 5, 10, or 15C under 0 (darkness) or 2 μmol·m–2·s–1 PPF (continuous lighting) supplied by fluorescent lamps (white light). Dry weight of the plantlets stored for 6 weeks at 5 or 10C in light was not significantly different from that of the plantlets before storage. Dry weight of the plantlets decreased as temperature increased and was maintained at higher levels in light than in darkness. Chlorophyll concentrations of the plantlets were higher at the lower temperatures. Chlorophyll fluorescence kinetics indicated higher activities of chlorophyll of the plantlets stored in light than in darkness. Lighting at as low as 2 μmol·m–2·s–1 PPF was important to preserve photosynthetic and regrowth abilities and dry weight of the plantlets during low-temperature storage.

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