Medium-term in vitro conservation of virus-free parthenocarpic tomato plants

2018 ◽  
Vol 54 (4) ◽  
pp. 392-398 ◽  
Author(s):  
Sota Koeda ◽  
Shotaro Matsumoto ◽  
Yuki Matsumoto ◽  
Rihito Takisawa ◽  
Koji Nishikawa ◽  
...  
Keyword(s):  
In Vitro Conservation ◽  
Medium Term ◽  
Tomato Plants

Medium-term in vitro conservation of Castanea spp. hybrid clones

2021 ◽  
Vol 34 (1) ◽  
pp. 127-137
Author(s):  
Filomena Gomes ◽  
Marta Clemente ◽  
Patricia Figueiredo ◽  
Rita Lourenço Costa
Keyword(s):  
In Vitro Conservation ◽  
Medium Term ◽  
Hybrid Clones

scholarly journals Medium Term Conservation of Several Carnation Accessions Via in Vitro Culture

2012 ◽  
Vol 13 (2) ◽  
pp. 174
Author(s):  
Kurniawan Budiarto ◽  
Budi Marwoto
Keyword(s):  
Low Temperature ◽  
Significant Variation ◽  
Root Formation ◽  
In Vitro Conservation ◽  
Medium Term ◽  
Low Temperature Storage ◽  
Two Factors ◽  
Temperature Storage

Sufficient genetic diversity is important in carnation breeding program. In vivo conservation of carnation germplasmis considered inefficient due to some technical and economical aspects. In vitro conservation was then, expectedto overcome the limitation of in vivo method. The research was conducted to find out the proper media for medium-term in vitro conservation of several carnation accessions in low temperature storage. A complete factorialexperiment with 25 replications was designed to accomplish the combination of two factors. The first factor wassix commercial carnation cultivars, namely Pink Maladi, Orange Triumph, Opera, Tundra, Yellow Liberty and PradoReffit. The second factor was the conservation media i.e. 1⁄2MS + DMSO 3% and 1⁄2MS + 3% DMSO + 3% sucrose andcontrol (MS 0+3% sucrose). The results showed that in vitro conservation of carnation in low temperature weresuccessfully conducted using 1⁄2MS+3% DMSO and 1⁄2MS+3% DMSO+3% sucrose without significant variation in allaccessions tested up to 10 and 12 months respectively. The increase of death plantlets, however, was detected onthe media of 1⁄2MS+3% DMSO after 6 months storage with significant decrease in viability hereafter. The existenceof sucrose in DMSO media induced root formation and plantlet resistance to low temperature storage.

scholarly journals Teknik Enkapsulasi Sederhana untuk Konservasi In vitro Jangka Menengah Tanaman Nenas (Ananas comosus) [Simple Encapsulation Technique for Medium Term Pineapple (Ananas comosus) In vitro Conservation]

2019 ◽  
Vol 29 (1) ◽  
pp. 1
Author(s):  
Riry Prihatini ◽  
Sri Hadiati
Keyword(s):  
Shelf Life ◽  
Sodium Alginate ◽  
Incubation Temperature ◽  
Genetic Material ◽  
Synthetic Seeds ◽  
In Vitro Conservation ◽  
Ananas Comosus ◽  
Medium Term ◽  
Tropical Fruit

<p>Konservasi in vitro tanaman nenas dilakukan untuk penyimpanan materi genetik sebelum dimanfaatkan. Penelitian ini dilaksanakan untuk mengembangkan teknik enkapsulasi yang dapat memperpanjang daya simpan benih sintetik nenas melalui perlakuan konsentrasi natrium alginat, suhu, dan media penyimpanan. Penelitian dilakukan di Laboratorium Kultur Jaringan, Balai Penelitian Tanaman Buah Tropika, mulai Januari hingga Desember 2017. Bahan yang digunakan adalah plantlet nenas aksesi 5X18(10). Penelitian dibagi menjadi dua subkegiatan. Metode yang digunakan pada subkegiatan pertama yaitu tunas mikro nenas dienkapulasi dengan metode tetes menggunakan natrium alginat 3% dan 4% serta penyimpanan dalam akuades steril dan tanpa media selama 30, 60, 120, dan 240 hari pada suhu 25oC. Penggunaan 4% natrium alginat dan media akuades steril dapat memperpanjang masa simpan benih sintetik nenas hingga 240 hari dengan daya regenerasi benih 100%. Pada subkegiatan kedua, perlakuan terbaik pada subkegiatan pertama dilanjutkan dengan perlakuan suhu penyimpanan 4oC. Benih sintetik nenas pada suhu penyimpanan tersebut hanya mampu bertahan hingga 60 hari, selebihnya tunas dalam benih menghitam dan tidak dapat ditumbuhkan kembali. Metode enkapsulasi untuk penyimpanan materi genetik yang dikembangkan dalam penelitian ini lebih sederhana dan efisien serta dapat diaplikasikan pada kegiatan konservasi in vitro jangka menengah tanaman nenas.</p><p><strong>Keywords</strong></p><p>Enkapsulasi; Konservasi; In vitro;  Tanaman nenas</p><p><strong>Abstract</strong></p><p>In vitro conservation of pineapple was conducted as preservation of genetic material before it was further utilized. This research was conducted to obtain encapsulation technique which expanded synthetic seeds shelf life by modifying concentration of sodium alginate, incubation media, and temperature. The research was conducted on Tissue Culture Laboratory of Indonesian Tropical Fruit Research Institute on January to December 2017. The materials which were used included pineapple micro shoots accessions 5X18(10). The research was divided into subactivities. The method which was applied on the first subactivity included encapsulation of pineapple micro shoots using drop method with sodium alginate 3% and 4%,incubation media sterile aquades and without media for 30, 60, 120, and 240 days on 25oC temperature.The use of 4% sodium alginate and sterile aquades incubation media prolonged the pineapple shelf life up to 240 days with 100% regeneration capability. On the second subactivity, the best treatment on the first activity was combined with 4oC incubation temperature. The pineapple synthetic seeds on this incubation temperature only survive up to 60 days, became blackening, and could not be regrowth. Encapsulation method which was developed on this study was simpler, more efficient, and able to be applied for medium term pineapple in vitro conservation.</p>

scholarly journals Medium-term and long-term in vitro conservation and safe international exchange of yam (Dioscorea spp.) germplasm

1998 ◽  
Vol 1 (2) ◽  
pp. 103-117 ◽  
Author(s):  
Bernard Malaurie ◽  
Marie-France Trouslot ◽  
Julien Berthaud ◽  
Mustapha Bousalem ◽  
Agnes Pinel ◽  
...  
Keyword(s):  
In Vitro Conservation ◽  
International Exchange ◽  
Medium Term

scholarly journals Zinc phosphate protects tomato plants against Pseudomonas syringae pv. tomato

2021 ◽  
Author(s):  
Mara Quaglia ◽  
Marika Bocchini ◽  
Benedetta Orfei ◽  
Roberto D’Amato ◽  
Franco Famiani ◽  
...  
Keyword(s):  
Disease Severity ◽  
Pseudomonas Syringae ◽  
Zinc Phosphate ◽  
Plant Defence ◽  
In Planta ◽  
Tomato Plants ◽  
Pathogenesis Related Protein ◽  
Defence Responses ◽  
Plant Defence Responses

AbstractThe purpose of this study was to determine whether zinc phosphate treatments of tomato plants (Solanum lycopersicum L.) can attenuate bacterial speck disease severity through reduction of Pseudomonas syringae pv. tomato (Pst) growth in planta and induce morphological and biochemical plant defence responses. Tomato plants were treated with 10 ppm (25.90 µM) zinc phosphate and then spray inoculated with strain DAPP-PG 215, race 0 of Pst. Disease symptoms were recorded as chlorosis and/or necrosis per leaf (%) and as numbers of necrotic spots. Soil treatments with zinc phosphate protected susceptible tomato plants against Pst, with reductions in both disease severity and pathogen growth in planta. The reduction of Pst growth in planta combined with significantly higher zinc levels in zinc-phosphate-treated plants indicated direct antimicrobial toxicity of this microelement, as also confirmed by in vitro assays. Morphological (i.e. callose apposition) and biochemical (i.e., expression of salicylic-acid-dependent pathogenesis-related protein PR1b1 gene) defence responses were induced by the zinc phosphate treatment, as demonstrated by histochemical and qPCR analyses, respectively. In conclusion, soil treatments with zinc phosphate can protect tomato plants against Pst attacks through direct antimicrobial activity and induction of morphological and biochemical plant defence responses.

scholarly journals Rhizosphere-enriched microbes as a pool to design synthetic communities for reproducible beneficial outputs

2019 ◽  
Author(s):  
Maria-Dimitra Tsolakidou ◽  
Ioannis A Stringlis ◽  
Natalia Fanega-Sleziak ◽  
Stella Papageorgiou ◽  
Antria Tsalakou ◽  
...  
Keyword(s):  
Antifungal Activity ◽  
Fungal Pathogens ◽  
Growth Parameters ◽  
Tomato Plants ◽  
Beneficial Effects ◽  
Negative Effect ◽  
Tomato Growth ◽  
In Vitro Antifungal Activity

Abstract Composts represent a sustainable way to suppress diseases and improve plant growth. Identification of compost-derived microbial communities enriched in the rhizosphere of plants and characterization of their traits, could facilitate the design of microbial synthetic communities (SynComs) that upon soil inoculation could yield consistent beneficial effects towards plants. Here, we characterized a collection of compost-derived bacteria, previously isolated from tomato rhizosphere, for in vitro antifungal activity against soil-borne fungal pathogens and for their potential to change growth parameters in Arabidopsis. We further assessed root-competitive traits in the dominant rhizospheric genus Bacillus. Certain isolated rhizobacteria displayed antifungal activity against the tested pathogens and affected growth of Arabidopsis, and Bacilli members possessed several enzymatic activities. Subsequently, we designed two SynComs with different composition and tested their effect on Arabidopsis and tomato growth and health. SynCom1, consisting of different bacterial genera, displayed negative effect on Arabidopsis in vitro, but promoted tomato growth in pots. SynCom2, consisting of Bacilli, didn't affect Arabidopsis growth, enhanced tomato growth and suppressed Fusarium wilt symptoms. Overall, we found selection of compost-derived microbes with beneficial properties in the rhizosphere of tomato plants, and observed that application of SynComs on poor substrates can yield reproducible plant phenotypes.

scholarly journals In vitro Conservation and Cryopreservation of Nandina domestica, an Outdoor Ornamental Shrub

2013 ◽  
Vol 41 (2) ◽  
pp. 638 ◽  
Author(s):  
Aylin OZUDOGRU ◽  
Diogo Pedrosa Corrêa Da SILVA ◽  
Ergun KAYA ◽  
Giuliano DRADI ◽  
Renato PAIVA ◽  
...  
Keyword(s):  
Liquid Nitrogen ◽  
Storage Temperature ◽  
Sucrose Concentration ◽  
Aluminum Foil ◽  
Shoot Tips ◽  
In Vitro Conservation ◽  
Shoot Cultures ◽  
Storage Medium ◽  
Droplet Vitrification

The study focused on an economically-important ornamental outdoor shrub, Nandina domestica, with the aims to (i) optimize an effective in vitro conservation method, and (ii) develop a cryopreservation protocol for shoot tips by the PVS2 vitrification and droplet-vitrification techniques. For in vitro conservation of shoot cultures, the tested parameters were sucrose content in the storage medium (30, 45, 60 g/L) and storage temperature (4 °C or 8 °C). Cryopreservation was performed by applying the PVS2 vitrification solution, in 2-ml cryovials or in drops over aluminum foil strips, for 15, 30, 60 or 90 min at 0 °C, followed by the direct immersion in liquid nitrogen of shoot tips. Results show that N. domestica shoots can be conserved successfully for 6 months at both the temperatures tested, especially when 60 g/L sucrose is used in the storage medium. However, conservation at 4 °C showed to be more appropriate, as hyperhydricity was observed in post-conservation of shoots coming from storage at 8 °C. As for cryopreservation, a daily gradual increase of sucrose concentration (from 0.25 to 1.0 M) produced better protection to the samples that were stored in liquid nitrogen. Indeed, with this sucrose treatment method, a 30-min PVS2 incubation time was enough to produce, 60 days after thawing, the best recovery (47% and 50%) of shoot tips, cryopreserved with PVS2 vitrification and droplet-vitrification, respectively.

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