Plant regeneration via indirect somatic embryogenesis and optimisation of genetic transformation in Coffea arabica L. cvs. Caturra and Catuaí

Propagation of Coffea arabica L. through direct and indirect somatic embryogenesis technique is promising for producing large number of coffee seedlings. The objectives of the research were to evaluate methods for direct and indirect somatic embryo-genesis induction of C. arabica var. Kartika. The explants were the youngest fully expanded leaves of arabica coffee. The evalu-ated medium was modified Murashige and Skoog (MS) medium supplemented with a combination of 2.26 µM 2,4-D + 4.54 or 9.08 µM thidiazuron; 4.52 µM 2,4-D + 4.54 or 9.08 µM thidiazuron; or 9.04 µM 2,4-D + 9.08 µM thidiazuron. Both calli (100 mg) and pre-embryos developed from the edge of leaf explants were subcultured into regeneration medium (half strength MS with modified vitamin, supplemented with kinetine 9.30 µM and adenine sulfate 40 mg L-1). The results showed coffee leaf explant cultured on medium containing 2.26 µM 2,4-D + 4.54 or 9.08 µM thidiazuron to induce direct somatic embriogenesis from explant, while that of 4.52 or 9.04 µM 2,4-D + 9.08 µM thidiazuron to induced indirect somatic embrio-genesis. The medium for calli induction from coffee by explants was medium supplemented with 4.52 or 9.04 µM 2,4-D in combination with 9.08 µM thidiazuron. On the other hand, the best medium for activation of induction of somatic embryos was MS medium supplemented with 9.04 µM 2,4-D + 9.08 µM thidiazuron. Based on this results, the first step for developing micropropagation for coffee has been resolved. The subsequent studies will be directed to evaluate agronomic performance of the derived planting materials.

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DIRECT AND INDIRECT SOMATIC EMBRYOGENESIS ON ARABICA COFFEE (Coffea arabica)

Indonesian Journal of Agricultural Science ◽

10.21082/ijas.v14n2.2013.p79-86 ◽

2013 ◽

Vol 14 (2) ◽

pp. 79 ◽

Cited By ~ 3

Author(s):

Meynarti Sari Dewi Ibrahim ◽

Rr. Sri Hartati ◽

Rubiyo Rubiyo ◽

Agus Purwito ◽

Sudarsono Sudarsono

Keyword(s):

Somatic Embryogenesis ◽

Coffea Arabica ◽

Leaf Explants ◽

Ms Medium ◽

Regeneration Medium ◽

Arabica Coffee ◽

Indirect Somatic Embryogenesis ◽

Half Strength ◽

Planting Materials ◽

Coffea Arabica L

Propagation of Coffea arabica L. through direct and indirect somatic embryogenesis technique is promising for producing large number of coffee seedlings. The objectives of the research were to evaluate methods for direct and indirect somatic embryo-genesis induction of C. arabica var. Kartika. The explants were the youngest fully expanded leaves of arabica coffee. The evalu-ated medium was modified Murashige and Skoog (MS) medium supplemented with a combination of 2.26 µM 2,4-D + 4.54 or 9.08 µM thidiazuron; 4.52 µM 2,4-D + 4.54 or 9.08 µM thidiazuron; or 9.04 µM 2,4-D + 9.08 µM thidiazuron. Both calli (100 mg) and pre-embryos developed from the edge of leaf explants were subcultured into regeneration medium (half strength MS with modified vitamin, supplemented with kinetine 9.30 µM and adenine sulfate 40 mg L-1). The results showed coffee leaf explant cultured on medium containing 2.26 µM 2,4-D + 4.54 or 9.08 µM thidiazuron to induce direct somatic embriogenesis from explant, while that of 4.52 or 9.04 µM 2,4-D + 9.08 µM thidiazuron to induced indirect somatic embrio-genesis. The medium for calli induction from coffee by explants was medium supplemented with 4.52 or 9.04 µM 2,4-D in combination with 9.08 µM thidiazuron. On the other hand, the best medium for activation of induction of somatic embryos was MS medium supplemented with 9.04 µM 2,4-D + 9.08 µM thidiazuron. Based on this results, the first step for developing micropropagation for coffee has been resolved. The subsequent studies will be directed to evaluate agronomic performance of the derived planting materials.

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Variation of nuclear DNA content during somatic embryogenesis and plant regeneration of Coffea arabica L. using cytophotometry

Plant Science ◽

10.1016/s0168-9452(02)00322-9 ◽

2003 ◽

Vol 164 (2) ◽

pp. 141-146 ◽

Cited By ~ 13

Author(s):

Svetlana E Zoriniants ◽

Alexander V Nosov ◽

Miriam Monforte-Gonzalez ◽

Marcela Mendes-Zeel ◽

Victor M Loyola-Vargas

Keyword(s):

Somatic Embryogenesis ◽

Plant Regeneration ◽

Dna Content ◽

Coffea Arabica ◽

Nuclear Dna ◽

Nuclear Dna Content ◽

Coffea Arabica L

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Comparison of Somatic Embryogenesis‐derived Coffee (Coffea arabica L.) Plantlets Regenerated in vitro or ex vitro: Morphological, Mineral and Water Characteristics

Annals of Botany ◽

10.1093/aob/mcf149 ◽

2002 ◽

Vol 90 (1) ◽

pp. 77-85 ◽

Cited By ~ 24

Author(s):

D. BARRY‐ETIENNE ◽

B. BERTRAND ◽

N. VASQUEZ ◽

H. ETIENNE

Keyword(s):

Somatic Embryogenesis ◽

Coffea Arabica ◽

Ex Vitro ◽

Water Characteristics ◽

Coffea Arabica L

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The effect of auxin 2,4-D and cytokinin 2-ip on direct somatic embryogenesis formation of Coffea arabica L. leaf explant

Pelita Perkebunan (a Coffee and Cocoa Research Journal) ◽

10.22302/iccri.jur.pelitaperkebunan.v27i2.146 ◽

2011 ◽

Vol 27 (2) ◽

Author(s):

Rina Arimarsetiowati

Keyword(s):

Tissue Culture ◽

Somatic Embryogenesis ◽

Coffea Arabica ◽

Callus Formation ◽

Culture Technique ◽

Plant Production ◽

Direct Somatic Embryogenesis ◽

Arabica Coffee ◽

Randomized Design ◽

Coffea Arabica L

One of the propagation technique for coffee plant production is tissue culture. Tissue culture technique for Coffea arabica L. faces some problems, mainly in the planlet formation regenerated from explants. The objective of this experiment was to examine the effect 2,4-D and 2-ip combination on the formation of direct somatic embryogenesis of Coffea arabica L. in leaves explant. Auxin (2,4-D) and cytokinin (2-ip) concentrations of, respectively, 1; 5 µM and 5; 10; 15; 20 were used as treatments. This research was conducted using completely randomized design with 10 replications. Observation to induce somatic embryos was done by quantitatively on number of callus from explant and number of embryogenic callus. Beside that, observation by qualitative descriptive was also done on deve lopment of embryogenesis. The results showed that Arabica coffee leaves explant of AS 2K clones could be induced in all medium combination except 5µM 2,4-D and 20µM 2-ip combination. Arabica coffee leaves explant of S 795, Sigararutang and AS 1 varieties could be induced in all medium combination. The highest frequency of callus formation was found in AS 2K, Sigararutang and AS 1 varieties on medium containing 1µM 2,4-D in combination with 10µM 2-ip, whereas for the S 795 variety on medium containing 5µM 2,4-D in combination with 10µM 2-ip. The highest frequency of embriogenic callus in all Arabica coffee variety could be reached on medium containing 5µM 2,4-D in combination with 15µM 2-ip. Key words : Coffea arabica L., somatic embryogenesis, 2,4-D, 2-ip, tissue culture, leaves, callus embryogenic.

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