scholarly journals A novel porcine mRNA differentially expressed in the Longissimus muscle tissues from Meishan and Large White pigs are associated with carcass traits and meat quality

2008 ◽  
Vol 17 (4) ◽  
pp. 559-567
Author(s):  
G. Liu ◽  
Y. Xiong
Keyword(s):  
Meat Quality ◽  
Carcass Traits ◽  
Differentially Expressed ◽  
Large White ◽  
Muscle Tissues

scholarly journals Comparison of PRKAG3 and RYR1 gene effect on carcass traits and meat quality in Slovenian commercial pigs

2010 ◽  
Vol 55 (No. 4) ◽  
pp. 149-159 ◽  
Author(s):  
M. Škrlep ◽  
T. Kavar ◽  
M. Čandek-Potokar
Keyword(s):  
Meat Quality ◽  
Carcass Traits ◽  
Drip Loss ◽  
Negative Effects ◽  
Large White ◽  
Maternal Line ◽  
Paternal Line ◽  
Genotype Frequencies ◽  
Subjective Score ◽  
Colour Parameters

The effect of polymorphisms at <I>PRKAG3</I> (<I>R200Q</I> and <I>I199V</I>) and <I>RYR1</I> (<I>R615C</I>) genes on carcass traits and meat quality was examined in a sample of 257 commercial pigs, crosses of Landrace × Large White as maternal line and Pietrain (<I>N</I> = 96), Pietrain × Landrace (<I>N</I> = 42) or Pietrain × Hampshire (<I>N</I> = 119) as paternal line. Pigs were genotyped (PCR-RFLP) and traits of interest were measured (which included carcass and ham weight, measurements of fatness, meatiness, ultimate pH, colour parameters and drip loss). The observed genotype frequencies at <I>PRKAG3</I> gene were 9.7%, 38.9%, 32.7%, 6.2% and 12.5% for <I>R/R-I/I, R/R-I/V, R/R-V/V, Q/R-I/V</I> and <I>Q/R-V/V</I> genotype, respectively. <I>RYR1</I> genotype frequencies were 57.2% for <I>N/N</I> and 42.8% for <I>N/n</I> genotype. Studied polymorphisms exhibited a significant effect on meat quality, but mainly an insignificant effect on carcass traits. No significant interaction between <I>PRKAG3</I> and <I>RYR1</I> was found. Carriers of <I>RYR1</I> mutant allele “<I>n</I>” had less intense <I>longissimus dorsi</I> muscle colour (subjective score, Minolta <I>L</I>* and <I>b</I>*) and higher drip loss. Regarding <I>PRKAG3</I>, the ultimate pH decreased and Minolta <I>L</I>*, <I>a</I>*, <I>b</I>* and drip loss increased in the following order: <I>R/R-I/I, R/R-I/V, R/R-V/V, Q/R-I/V</I> and <I>Q/R-V/V</I>, according to the presence of <I>199I</I> and absence of <I>200Q</I> alleles. The study shows that the <I>I199V</I> polymorphism is an important source of variation in pigs free of <I>200Q</I>. In particular the <I>199I</I> proves beneficial for meat quality. The results of combining the <I>RYR1</I> and <I>PRKAG3</I> genotypes indicate that <I>R/R-I/I</I> genotype could be used in counterbalancing the negative effects of “<I>n</I>” allele on meat quality.<B></B>

Isolation, identification and expression profile of fourteen differentially expressed sequence tags in the longissimus dorsi muscle tissues from Meishan, Meishan×Large White cross and Large White pigs

2007 ◽  
Vol 4 (1) ◽  
pp. 9-14
Author(s):  
Liu Yong-Gang ◽  
Xiong Yuan-Zhu ◽  
Zuo Bo ◽  
Jiang Si-Wen ◽  
Deng Chang-Yan ◽  
...  
Keyword(s):  
Expression Profile ◽  
Differential Display ◽  
Expressed Sequence Tags ◽  
Differentially Expressed ◽  
Longissimus Dorsi ◽  
Large White ◽  
Longissimus Dorsi Muscle ◽  
Dorsi Muscle ◽  
Muscle Tissues ◽  
Expressed Sequence

AbstractIn order to detect the molecular mechanism of heterosis in pigs, an mRNA differential display (DD) technique was performed to investigate the differences in gene expression in the longissimus dorsi muscle tissues from Meishan, Meishan×Large White cross and Large White pigs. Fourteen expressed sequence tags (ESTs), differentially expressed between the hybrid and purebred pigs, were isolated and identified through semi-quantitative reverse transcriptase-polymerase chain reaction (RT-PCR). Nucleotide sequence analysis revealed that the 14 ESTs are not homologous to any of the known genes or ESTs. These novel ESTs were then deposited in the GenBank database. Tissue expression profile analysis showed that the ESTs were expressed in most tissues, including heart, spleen, liver, kidney, small intestine, ovary and lung, and this also implied that these genes must be important for the life process. Our results indicate the diversity of differential display of genes between the hybrids and purebreds in the Meishan×Large White cross combination. Results also suggest that heterosis in pigs might be derived from the differential expression of many indispensable genes in specific life phases.

scholarly journals Identification of CircRNA-Associated ceRNA Networks Using Longissimus Thoracis of Pigs of Different Breeds and Growth Stages

2021 ◽  
Author(s):  
Xiaona Zhuang ◽  
Zekun Lin ◽  
Junyi Luo ◽  
Ting Chen ◽  
Qianyun Xi ◽  
...  
Keyword(s):  
Meat Quality ◽  
Fiber Type ◽  
Biological Activities ◽  
Differentially Expressed ◽  
Integrated Analysis ◽  
Growth Stages ◽  
Muscle Tissues ◽  
Genome Wide ◽  
Key Factor ◽  
Pathway Analyses

Abstract Background: Long-term artificial selection for growth rate and lean meat rate has eventually led to meat quality deterioration. Muscle fiber type is a key factor that markedly affects meat quality. circRNAs have been reported to participate in diverse biological activities, including myofiber growth and development; thus, we herein compared porcine circRNA transcriptome between oxidative and glycolytic muscle tissues. Results: Longissimus thoracis muscle tissues were obtained from Lantang and Landrace pigs at birth (LT1D and LW1D, respectively) and 90 postnatal days (LT90D and LW90D, respectively). Hematoxylin and eosin staining and quantitative real-time PCR revealed that all structural traits of the muscle showed large variations between different breeds and growth stages. In total, 329 known miRNAs and 42,081 transcript candidates were identified; 6,962 differentially expressed transcripts were found to play a key role in myogenesis by gene ontology and Kyoto Encyclopedia of Genes and Genomes pathway analyses. In addition, 3,352 circRNAs were identified using five predicting algorithms, and 104 circRNA candidates were differentially expressed. Integrated analysis of differentially expressed miRNAs, mRNAs, and circRNAs led to the identification of 777, 855, and 22 convincing ceRNA interactions in LT1D vs. LT90D, LW1D vs. LW90D, and LT90D vs. LW90D, respectively. Finally, we identified a circRNA candidate circKANSL1L, which showed high homology between mice and pigs, and it was found to inhibit the proliferation of C2C12 cells but promote their differentiation.Conclusions: We identified genome-wide circRNAs in 0- and 90-day-old Lantang and Landrace pigs by RNA-seq and found that circRNAs were abundant, differentially expressed, and associated with myogenesis. Our results should serve as a reference for future studies on pork quality.

scholarly journals Isolation, Sequence Analysis and Expression Profile of a Novel Swine Gene Differentially Expressed in the Longissimus Dorsi Muscle Tissues from Landrace×Large White Cross-combination

2005 ◽  
Vol 37 (3) ◽  
pp. 186-191 ◽  
Author(s):  
Yong-Gang Liu ◽  
Yuan-Zhu Xiong ◽  
Chang-Yan Deng
Keyword(s):  
Carbonic Anhydrase ◽  
Differentially Expressed ◽  
Longissimus Dorsi ◽  
Cross Combination ◽  
Large White ◽  
Phylogenetic Tree Analysis ◽  
Longissimus Dorsi Muscle ◽  
Carbonic Anhydrase Iii ◽  
Dorsi Muscle ◽  
Muscle Tissues

Abstract The mRNA differential display technique was performed to investigate the differences in gene expression in the Longissimus dorsi muscle tissues from Landrace×Large White cross-combination. One novel gene that was differentially expressed was identified using semi-quantitative reverse transcriptase-polymerase chain reaction (RT-PCR) and its complete cDNA sequence was obtained using the rapid amplification of cDNA ends (RACE) method. The nucleotide sequence of the gene is not homologous to any of the known porcine genes. The sequence prediction analysis revealed that the open reading frame of this gene encodes a protein of 260 amino acids that contains the putative conserved domain of the carbonic anhydrase, and this protein has high homology with the carbonic anhydrase III (CA-III) of four species-mouse (91%), horse (91%), rat (89%) and human (86%)–so that it can be defined as swine carbonic anhydrase III. The phylogenetic tree analysis revealed that the swine CA-III has a closer genetic relationship with the horse CA-III than with those of mouse, rat and human. The tissue expression analysis indicated that the swine CA-III gene is generally expressed in most tissues. Our experiment is the first to establish the primary foundation for further research on the swine CA-III gene.

scholarly journals Comparison of carcass traits, meat quality and expressions of MyHCs in muscles between Mashen and Large White pigs

2019 ◽  
Vol 18 (1) ◽  
pp. 1410-1418 ◽  
Author(s):  
Xiaohong Guo ◽  
Benyuan Qin ◽  
Xiaofen Yang ◽  
Jingmin Jia ◽  
Jiaoyan Niu ◽  
...  
Keyword(s):  
Meat Quality ◽  
Carcass Traits ◽  
Large White

A novel porcine gene, POT1, differentially expressed in the longissimus muscle tissues from Wujin and Large White pigs

Cytokine ◽  
2012 ◽  
Vol 59 (1) ◽  
pp. 22-26 ◽  
Author(s):  
Wang Jin Yong ◽  
Lan Jing ◽  
Zhao Jiugang ◽  
Chen Lei ◽  
Liu Yonggang
Keyword(s):  
Differentially Expressed ◽  
Longissimus Muscle ◽  
Large White ◽  
Muscle Tissues ◽  
Porcine Gene

scholarly journals Effects of Dietary Perilla Cake Supplementation in Growing Pig on Productive Performance, Meat Quality, and Fatty Acid Profiles

Animals ◽  
2021 ◽  
Vol 11 (11) ◽  
pp. 3213
Author(s):  
Chaiwat Arjin ◽  
Chanmany Souphannavong ◽  
Rakkiat Norkeaw ◽  
Niraporn Chaiwang ◽  
Supamit Mekchay ◽  
...  
Keyword(s):  
Fatty Acid Composition ◽  
Fatty Acid ◽  
Meat Quality ◽  
Acid Composition ◽  
Average Daily Gain ◽  
Carcass Traits ◽  
Basal Diet ◽  
Experimental Period ◽  
Large White ◽  
Growing Pig

The objective of this study was to determine the effect of perilla cake (PC) supplementation in a growing pig diet on overall growing performance, meat quality, and fatty acid profile. A total of 24 barrow grower crossbred pigs (Large White × Landrace) × Duroc with an initial average body weight of 26.33 kg were fed with a basal diet supplemented with PC at 0%, 5%, and 10% in (PC0, PC5, and PC10, respectively) for 12 weeks. At the end of the experimental period, pigs were slaughtered to determine carcass traits and meat quality. Back fat, abdominal fat, and longissimus dorsi (LD) muscle were collected to investigate fatty acid composition. The results show that the average daily gain (ADG) in the PC10 significantly increased. However, PC supplementation did not influence carcass traits and meat quality except the color as described by lightness (L*). Dietary PC supplementation significantly increased the α-linolenic acid (ALA, C18:3 cis-9, 12, 15), whereas n6/n3 ratio decreased significantly in all tissues investigated. Thus, it can be concluded that the supplementation of PC in growing pig diet is a potential way to increase the fatty acid composition to that required for healthier meat.

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