Lenilton Silva da Silveira-Júnior
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Franklin Souza-Silva
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Bernardo Acácio Santini Pereira
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Léa Cysne-Finkelstein
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Geraldo Barroso Cavalcanti Júnior
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A surface plasmon resonance- (SPR-) based recognition method applying H-2Ld:Ig/peptides complexes for ex vivo monitoring cellular immune responses during murine infection withLeishmania (Leishmania) amazonensisis described. Lymphocytes from lesion-draining popliteal lymph nodes were captured on a carboxylated sensor chip surface previously functionalized with H-2Ld:Ig (DimerX) protein bound to synthetic peptides derived from the COOH-terminal region of cysteine proteinase B ofL. (L.) amazonensis.In computational analysis, these peptides presented values of kinetic constants favorable to form complexes with H-2Ldat neutral pH, with a Gibbs free energyΔG°<0. The assayed DimerX:peptide complexes presented the property of attaching to distinct T lymphocytes subsets, obtained from experimentally infected BALB/c mice, in each week of infection, thus indicating a temporal variation in specific T lymphocytes populations, each directed to a different COOH-terminal region-derived peptide. The experimental design proposed herein is an innovative approach for cellular immunology studies of a neglected disease, providing a useful tool for the analysis of specific T lymphocytes subsets.