scholarly journals Hepatic Microhaemocirculation Dynamics in Experimental Extrahepatic Cholestasis

2020 ◽  
Vol 30 (6) ◽  
pp. 45-50
Author(s):  
K. Kh. Akhmedov ◽  
M. A. Ergashev ◽  
A. N. Meliboboyev ◽  
E. H. Boltayev
Keyword(s):  
Bile Duct ◽  
Common Bile Duct ◽  
Male Rats ◽  
Mixed Group ◽  
Hepatic Circulation ◽  
Hepatic Parenchyma ◽  
Extrahepatic Cholestasis ◽  
Toxic Impact ◽  
White Mongrel

Aim. Investigation of hepatic microhaemocirculation effects in experimental extrahepatic cholestasis.Materials and Methods. Experiments were carried out with a mixed group of 48 white mongrel male rats. Extrahepatic cholestasis was modelled in rats with via the ligation of common bile duct.Results. Animals with ligated common bile duct showed abnormalities in peripheral hepatic circulation manifested by a complex of intravascular, vascular and paravascular changes, hepatic angioarchitectonics disturbance, a marked growth of parenchymal dystrophic lesions in the form of post-haemorrhagic foci. The disturbance was pronounced towards a virtual degradation of hepatic parenchyma and the growth of “numb” zones with ceased circulation.Conclusion. The trials revealed microcirculatory changes in the liver associated with rheological disturbance of blood and, subsequently, the toxic impact of failed metabolism.

scholarly journals Morphological features of the wall of common bile duct under the conditions of experimental opioid exposure

2020 ◽  
Vol 26 (2) ◽  
pp. 26-31
Author(s):  
L.R. Mateshuk-Vatseba ◽  
I.I. Hirniak ◽  
U.Y. Pidvalna
Keyword(s):  
Bile Duct ◽  
Common Bile Duct ◽  
Bile Ducts ◽  
White Male ◽  
Acute Cholangitis ◽  
Medical Science ◽  
Male Rats ◽  
Pathological Changes ◽  
White Rats ◽  
The Common

The morphological condition of the bile ducts remains one of the most important problems of modern medical science. In order to obtain an analgesic effect in patients with acute cholangitis, opioids are often used. However, information on the effectiveness of opioids in the treatment of pathological conditions of the bile ducts is contradictory. The rapidly progressive destruction of the intrahepatic bile ducts associated with the use of narcotic agents has been described. Further study of the effect of opioids on the structural organization of the common bile duct is relevant. In order to establish the morphological state of the common bile duct under conditions of long-term opioid exposure, a study was performed on 24 sexually mature white male rats, aged 3.5-5.0 months and weighing 180-200 g, which were injected intramuscularly with Nalbuphine for 6 weeks. The study material is represented by histological specimens of the common bile duct of white rats. The “Aver Media” computer system was used to photograph microspecimens. The “ImageJ” computer program was used to measure the diameter of the lumen and the wall thickness of the common bile duct. After 2 weeks of Nalbuphine administration to white rats, plethora of wall microvessels and a significant increase in the longitudinal diameter of the lumen of the common bile duct were observed. After 4 weeks of the experiment, the common bile duct was dilated, the transverse and longitudinal diameters of its lumen almost doubled, pathological changes in its wall had all the signs of inflammation. In the later stages of the experiment (introduction of Nalbuphine for 6 weeks), the pathological changes increased and manifested by destructuring the wall of the common bile duct, disorganization of cholangiocytes, thinning of the cell layer due to detachment of cholangiocytes, polymorphism of their nuclei, destruction of intercellular junctions, stratification of its own plate, vacuolar dystrophy of the muscular membrane “varicose” expansion of venules, significant smooth muscle hyperplasia of arterioles, the presence of perivascular lymphocytic infiltrates in the duct wall.

scholarly journals Effects of plastic stenting in commom bile duct of rats: a quantitative reaction analysis using collagen and elastin morphometry

2010 ◽  
Vol 25 (2) ◽  
pp. 153-157 ◽  
Author(s):  
Everson Luiz de Almeida Artifon ◽  
Fabio Pinatel Lopasso ◽  
Gustavo Brazuna Moura ◽  
Fernando Augusto Mardiros Herbella Fernandes ◽  
Paulo Sakai ◽  
...  
Keyword(s):  
Bile Duct ◽  
Common Bile Duct ◽  
Bile Ducts ◽  
Male Rats ◽  
Biliary Stenting ◽  
Midline Laparotomy ◽  
Plastic Stent ◽  
The Common ◽  
Sirius Red ◽  
Elastin Deposition

PURPOSE: To evaluate the effects of biliary stenting in rats through analysis of collagen and elastin deposition in the bile ducts. METHODS: Twenty male rats underwent midline laparotomy, duodenotomy and transampullary stenting of the common bile duct with a 22Fr plastic stent. Animals were randomized in 4 groups, with 5 components in each: (I) control, (II) biliary stenting for 7 days, (III) biliary stenting for 14 days, and (IV) biliary stenting for 30 days. Sections of the common bile duct were stained using Hematoxylin Eosin; Sirius Red to quantitate the amount of collagen present; and Weigert's Resorcin-fuchsin to quantitate the amount of elastin present. RESULTS: The percentage of area stained for collagen was 13.4; 21.5; 29.5 and 32.8, for groups I to IV, respectively. The percentage of area stained for elastin fibers was 7.0; 5.2; 4.0 and 2.9, for groups I to IV, respectively. Collagen/Elastin ratio was 2.4; 5.1; 11.0 and 14.4 for groups I to IV, respectively. CONCLUSIONS: The biliary stenting leads to collagen and elastin deposition in the bile ducts; and collagen deposition and collagen/elastin ratio are proportional to the period of stenting.

scholarly journals Neuroglobin in Neurons of the Frontal and Parietal Cortex of White Rats in Cholestasis

2020 ◽  
Vol 9 (1) ◽  
pp. 24-29
Author(s):  
S. V. Emel'yanchik ◽  
O. A. Karnyushko ◽  
S. M. Zimatkin
Keyword(s):  
Bile Duct ◽  
Common Bile Duct ◽  
Parietal Cortex ◽  
Pyramidal Neurons ◽  
White Male ◽  
Male Rats ◽  
Control Group ◽  
White Rats ◽  
The Common ◽  
Cytophotometric Analysis

The aim of the study was to investigate the distribution and content of neuroglobin in the pyramidal neurons of the frontal and parietal cortex of white rats during simulated cholestasis.Material and methods. The study included 60 outbred white male rats weighed 200–250 g. Cholestasis was simulated by ligation of the common bile duct in the porta hepatis area (main group, n=30). Animals of the control group (n=30) were performed a false operation preserving physiological bile outflow. Sections of the frontal and parietal cortex of white rats were selected for investigation. The content and distribution of neuroglobin was detected immunohistochemically on paraffin sections using mouse monoclonal primary antibodies Anti- Neuroglobin antibody (Abcam). Cytophotometric analysis was used to perform quantitative assessment of the content of the studied molecular marker; after that statistical analysis was performed.Results. It was found that the content of neuroglobin in the pericarions of neurons of the frontal and parietal cortex modified in a wave-like mode. After ligation of the common bile duct, the content of neuroglobin significantly decreased in 2, 10 and 45 days (minimum on the 10th day) in the frontal cortex and in 90 days in the parietal cortex, and increased in 5 and 20 days of the experiment (maximum on the 20th day).Conclusion. In cholestasis, the content of neuroglobin in the pericarions of neurons of the frontal and parietal cortex modifies in waves: a decrease is observed on the 2nd, 10th, 45th day; an increase is observed on the 5th and 20th day; a recovery is observed on the 90th day.

scholarly journals Expression of protein c-fos in cerebellum neurons of rats at cholestasis

2019 ◽  
Vol 16 (2) ◽  
pp. 135-141
Author(s):  
S. V. Emelyanchik ◽  
O. A. Karniushko ◽  
S. M. Zimatkin
Keyword(s):  
Nervous System ◽  
Bile Duct ◽  
Common Bile Duct ◽  
Bile Flow ◽  
Protein C ◽  
White Male ◽  
Male Rats ◽  
Experimental Animals ◽  
The Common

Cholestasis (disturbance of the liver bile outflow into the duodenum) is a frequent complication of cholelithiasis and other pathologies of the hepatobiliary system. In this case, all metabolism types are disturbances and all body organs and systems, including the nervous system, are affected. The objective of the study was to establish changes in the c-fos immunoreactivity in the cerebellum neurons of rats at different time after modeling subhepatic cholestasis. In this work, we used a material of 60 white male rats 200–250 g in weight. In experimental animals, the ligation of the common bile duct was carried out, in control animals – a false operation while maintaining a physiological bile flow in the duodenum throughout the experiment. Subhepatic cholestasis in rats is accompanied by an increase in the cortex cerebellum on the 2–20th days after the ligation of the common bile duct of the number of neurons with the increased c-fos-immunoreactivity, with the maximum one on the 10th day of the experiment. In surviving animals, after the removal of cholestasis in the long term (45–90 days), the number of neurons with the increased c-fos immunoreactivity, as well as the expression of this protein in neurons is normalized.

scholarly journals JAUNDICE IN MICE DUE TO ANOMALIES OF THE BILIARY TRACT

1955 ◽  
Vol 101 (2) ◽  
pp. 119-128
Author(s):  
Alwin M. Pappenheimer ◽  
F. S. Cheever ◽  
Herman Salk
Keyword(s):  
Bile Duct ◽  
Common Bile Duct ◽  
Gall Bladder ◽  
Terminal Segment ◽  
Gastric Epithelium ◽  
Biliary Cirrhosis ◽  
Hepatic Parenchyma ◽  
Inflammatory Lesions ◽  
The Common ◽  
Hepatic Lesions

There has been described a previously unrecognized disease of mice, characterized by progressive jaundice, first appearing during the nursing period. This has been shown to be due to congenital absence of the terminal segment of the common bile duct, or to the absence of intrahepatic ducts. In the former case, there is distension of the cystic and hepatic ducts, and of the gall bladder, with mucoid material. Biliary cirrhosis and infarct-like areas of necrosis are commonly found in the liver. The cause of the necroses has not been positively determined, but it is suggested that they result from defective arteriolization of the hepatic parenchyma. Inflammatory lesions of the biliary passages, when present, are attributed to secondary bacterial infection. Protozoan-like parasites were present in the gastric epithelium of all mice examined. Their relationship to the biliary and hepatic lesions is as yet undetermined.

Common Bile Duct Pressures

1957 ◽  
Vol 32 (6) ◽  
pp. 1122-1130 ◽  
Author(s):  
John R. Kelsey ◽  
Earl F. Beard
Keyword(s):  
Bile Duct ◽  
Common Bile Duct
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