Expression of protein c-fos in cerebellum neurons of rats at cholestasis

Cholestasis (disturbance of the liver bile outﬂow into the duodenum) is a frequent complication of cholelithiasis and other pathologies of the hepatobiliary system. In this case, all metabolism types are disturbances and all body organs and systems, including the nervous system, are aﬀected. The objective of the study was to establish changes in the c-fos immunoreactivity in the cerebellum neurons of rats at diﬀerent time after modeling subhepatic cholestasis. In this work, we used a material of 60 white male rats 200–250 g in weight. In experimental animals, the ligation of the common bile duct was carried out, in control animals – a false operation while maintaining a physiological bile ﬂow in the duodenum throughout the experiment. Subhepatic cholestasis in rats is accompanied by an increase in the cortex cerebellum on the 2–20th days after the ligation of the common bile duct of the number of neurons with the increased c-fos-immunoreactivity, with the maximum one on the 10th day of the experiment. In surviving animals, after the removal of cholestasis in the long term (45–90 days), the number of neurons with the increased c-fos immunoreactivity, as well as the expression of this protein in neurons is normalized.

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Morphological features of the wall of common bile duct under the conditions of experimental opioid exposure

Reports of Morphology ◽

10.31393/morphology-journal-2020-26(2)-04 ◽

2020 ◽

Vol 26 (2) ◽

pp. 26-31

Author(s):

L.R. Mateshuk-Vatseba ◽

I.I. Hirniak ◽

U.Y. Pidvalna

Keyword(s):

Bile Duct ◽

Common Bile Duct ◽

Bile Ducts ◽

White Male ◽

Acute Cholangitis ◽

Medical Science ◽

Male Rats ◽

Pathological Changes ◽

White Rats ◽

The Common

The morphological condition of the bile ducts remains one of the most important problems of modern medical science. In order to obtain an analgesic effect in patients with acute cholangitis, opioids are often used. However, information on the effectiveness of opioids in the treatment of pathological conditions of the bile ducts is contradictory. The rapidly progressive destruction of the intrahepatic bile ducts associated with the use of narcotic agents has been described. Further study of the effect of opioids on the structural organization of the common bile duct is relevant. In order to establish the morphological state of the common bile duct under conditions of long-term opioid exposure, a study was performed on 24 sexually mature white male rats, aged 3.5-5.0 months and weighing 180-200 g, which were injected intramuscularly with Nalbuphine for 6 weeks. The study material is represented by histological specimens of the common bile duct of white rats. The “Aver Media” computer system was used to photograph microspecimens. The “ImageJ” computer program was used to measure the diameter of the lumen and the wall thickness of the common bile duct. After 2 weeks of Nalbuphine administration to white rats, plethora of wall microvessels and a significant increase in the longitudinal diameter of the lumen of the common bile duct were observed. After 4 weeks of the experiment, the common bile duct was dilated, the transverse and longitudinal diameters of its lumen almost doubled, pathological changes in its wall had all the signs of inflammation. In the later stages of the experiment (introduction of Nalbuphine for 6 weeks), the pathological changes increased and manifested by destructuring the wall of the common bile duct, disorganization of cholangiocytes, thinning of the cell layer due to detachment of cholangiocytes, polymorphism of their nuclei, destruction of intercellular junctions, stratification of its own plate, vacuolar dystrophy of the muscular membrane “varicose” expansion of venules, significant smooth muscle hyperplasia of arterioles, the presence of perivascular lymphocytic infiltrates in the duct wall.

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Neuroglobin in Neurons of the Frontal and Parietal Cortex of White Rats in Cholestasis

Journal of Anatomy and Histopathology ◽

10.18499/2225-7357-2020-9-1-24-29 ◽

2020 ◽

Vol 9 (1) ◽

pp. 24-29

Author(s):

S. V. Emel'yanchik ◽

O. A. Karnyushko ◽

S. M. Zimatkin

Keyword(s):

Bile Duct ◽

Common Bile Duct ◽

Parietal Cortex ◽

Pyramidal Neurons ◽

White Male ◽

Male Rats ◽

Control Group ◽

White Rats ◽

The Common ◽

Cytophotometric Analysis

The aim of the study was to investigate the distribution and content of neuroglobin in the pyramidal neurons of the frontal and parietal cortex of white rats during simulated cholestasis.Material and methods. The study included 60 outbred white male rats weighed 200–250 g. Cholestasis was simulated by ligation of the common bile duct in the porta hepatis area (main group, n=30). Animals of the control group (n=30) were performed a false operation preserving physiological bile outflow. Sections of the frontal and parietal cortex of white rats were selected for investigation. The content and distribution of neuroglobin was detected immunohistochemically on paraffin sections using mouse monoclonal primary antibodies Anti- Neuroglobin antibody (Abcam). Cytophotometric analysis was used to perform quantitative assessment of the content of the studied molecular marker; after that statistical analysis was performed.Results. It was found that the content of neuroglobin in the pericarions of neurons of the frontal and parietal cortex modified in a wave-like mode. After ligation of the common bile duct, the content of neuroglobin significantly decreased in 2, 10 and 45 days (minimum on the 10th day) in the frontal cortex and in 90 days in the parietal cortex, and increased in 5 and 20 days of the experiment (maximum on the 20th day).Conclusion. In cholestasis, the content of neuroglobin in the pericarions of neurons of the frontal and parietal cortex modifies in waves: a decrease is observed on the 2nd, 10th, 45th day; an increase is observed on the 5th and 20th day; a recovery is observed on the 90th day.

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THE EFFECT OF LIGATION OF THE COMMON BILE DUCT ON CHOLESTEROL SYNTHESIS IN THE RAT

Journal of Experimental Medicine ◽

10.1084/jem.99.1.43 ◽

1954 ◽

Vol 99 (1) ◽

pp. 43-53 ◽

Cited By ~ 52

Author(s):

Donald S. Fredrickson ◽

Alden V. Loud ◽

Beverly T. Hinkelman ◽

Henny S. Schneider ◽

Ivan D. Frantz

Keyword(s):

Bile Duct ◽

Common Bile Duct ◽

Bile Ducts ◽

Cholesterol Synthesis ◽

Intact Animal ◽

Experimental Animals ◽

Liver Slices ◽

The Common ◽

Ad Libitum

The effect on cholesterol synthesis of ligation of the common bile duct was studied in the rat. The bile ducts of rats were ligated; 24 to 48 hours later, estimates of the rate of cholesterol synthesis were made, either by injection of labelled water or acetate into the intact animal, or by incubation of slices or homogenates of the liver in the presence of 1-C14-acetate. These various criteria all indicated that cholesterol synthesis was increased following ligation of the bile duct. The average ratios of the rate of synthesis in the experimental animals to that in the controls were as follows: 1. Synthesis from C14-carboxyl-labelled acetate: (a) in the intact rat fed ad libitum, 19; (b) in liver slices from the fasted rat, 23; (c) in liver slices from the rat fed ad libitum, 4; (d) in cell-free homogenates from the fasted rat, >27; (e) in cell-free homogenates from the rat fed ad libitum, 17. 2. Synthesis from tritium-labelled water in the intact rat fed ad libitum, 4.

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Sump Syndrome: A Rare Long-Term Complication of Choledochoduodenostomy

Case Reports in Gastroenterology ◽

10.1159/000477335 ◽

2017 ◽

Vol 11 (2) ◽

pp. 428-433 ◽

Cited By ~ 6

Author(s):

Hrudya Abraham ◽

Sajan Thomas ◽

Amit Srivastava

Keyword(s):

Bile Duct ◽

Common Bile Duct ◽

Endoscopic Retrograde Cholangiopancreatography ◽

Clinical Manifestations ◽

Rare Condition ◽

Endoscopic Retrograde ◽

Distal Common Bile Duct ◽

History Of ◽

The Common

Biliary sump syndrome is a rare condition. It is seen as a rare long-term complication in patients with a history of a side-to-side choledochoduodenostomy. In the era before endoscopic retrograde cholangiopancreatography, side-to-side choledochoduodenostomy was a common surgical procedure for the management of biliary obstruction. In the setting of a side-to-side choledochoduodenostomy, the bile does not drain through the distal common bile duct anymore. Therefore, the part of the common bile duct distal from the choledochoduodenostomy anastomosis consequently transforms into a poorly drained reservoir, making this so-called “sump” prone to accumulation of debris. These patients are prone to cholangitis. We present a 64-year-old man with a history of side-to-side choledochoduodenostomy who presented with manifestations of cholangitis. An endoscopic retrograde cholangiopancreatography confirmed a diagnosis of sump syndrome. The etiology, clinical manifestations, and treatment of biliary sump syndrome are discussed in this article.

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Effects of plastic stenting in commom bile duct of rats: a quantitative reaction analysis using collagen and elastin morphometry

Acta Cirurgica Brasileira ◽

10.1590/s0102-86502010000200006 ◽

2010 ◽

Vol 25 (2) ◽

pp. 153-157 ◽

Cited By ~ 1

Author(s):

Everson Luiz de Almeida Artifon ◽

Fabio Pinatel Lopasso ◽

Gustavo Brazuna Moura ◽

Fernando Augusto Mardiros Herbella Fernandes ◽

Paulo Sakai ◽

...

Keyword(s):

Bile Duct ◽

Common Bile Duct ◽

Bile Ducts ◽

Male Rats ◽

Biliary Stenting ◽

Midline Laparotomy ◽

Plastic Stent ◽

The Common ◽

Sirius Red ◽

Elastin Deposition

PURPOSE: To evaluate the effects of biliary stenting in rats through analysis of collagen and elastin deposition in the bile ducts. METHODS: Twenty male rats underwent midline laparotomy, duodenotomy and transampullary stenting of the common bile duct with a 22Fr plastic stent. Animals were randomized in 4 groups, with 5 components in each: (I) control, (II) biliary stenting for 7 days, (III) biliary stenting for 14 days, and (IV) biliary stenting for 30 days. Sections of the common bile duct were stained using Hematoxylin Eosin; Sirius Red to quantitate the amount of collagen present; and Weigert's Resorcin-fuchsin to quantitate the amount of elastin present. RESULTS: The percentage of area stained for collagen was 13.4; 21.5; 29.5 and 32.8, for groups I to IV, respectively. The percentage of area stained for elastin fibers was 7.0; 5.2; 4.0 and 2.9, for groups I to IV, respectively. Collagen/Elastin ratio was 2.4; 5.1; 11.0 and 14.4 for groups I to IV, respectively. CONCLUSIONS: The biliary stenting leads to collagen and elastin deposition in the bile ducts; and collagen deposition and collagen/elastin ratio are proportional to the period of stenting.

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Effect of insulin on canalicular bile formation

American Journal of Physiology-Legacy Content ◽

10.1152/ajplegacy.1976.231.1.40 ◽

1976 ◽

Vol 231 (1) ◽

pp. 40-43 ◽

Cited By ~ 15

Author(s):

RS Jones

Keyword(s):

Bile Duct ◽

Common Bile Duct ◽

Intravenous Infusion ◽

Bile Flow ◽

Sodium Taurocholate ◽

Duodenal Fistula ◽

Bile Formation ◽

Biliary Clearance ◽

The Common ◽

Bicarbonate Output

Mongrel dogs were prepared by cholecystectomy, ligation of the lesser pancreatic duct, and insertion of gastric and duodenal cannulas. The common bile duct was cannulated through the duodenal fistula. After bile flow had been stabilized by intravenous infusion of sodium taurocholate the dogs were given an intravenous injection of insulin or 0.9% NaCl (control). Insulin caused marked increases in bile flow, chloride output, and biliary clearance of erythritol and small increases in bicarbonate output and bile salt output. The increased erythritol clearance indicates that canalicular secretion contributes to insulin choleresis in dogs.

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