NTP Research Report on Synthetic Turf/Recycled Tire Crumb Rubber: Characterization of the Biological Activity of Crumb Rubber In Vitro

Two novel cyanido-complexes having very significant antibacterial, antifungal and anticancer activities in vitro were synthesized and characterized using various techniques.

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Biological characterization of charge isomers of human growth hormone

Acta Endocrinologica ◽

10.1530/acta.0.1180014 ◽

1988 ◽

Vol 118 (1) ◽

pp. 14-21 ◽

Cited By ~ 11

Author(s):

A. Skottner ◽

A. Forsman ◽

B. Skoog ◽

J. L. Kostyo ◽

C. M. Cameron ◽

...

Keyword(s):

Biological Activity ◽

Biological Activities ◽

Human Growth ◽

Proteolytic Processing ◽

Biological Characterization ◽

Growth Promoting ◽

Rat Adipose Tissue ◽

Insulin Like Activity

Abstract. Since deamidation of the human GH molecule may alter the manner and extent to which the hormone is cleaved by proteases, and since it has been repeatedly suggested that proteolytic processing is required for the expression of certain of the activities of GH, the present study was conducted to determine whether the biological activity profiles of more acidic forms of human GH are altered. Three charge isomers, GH-b, GH-c and GH-d, representing primarily deamidated forms, were isolated from a native human GH preparation (Crescormon®) in amounts adequate for characterization of their biological activities. All three were essentially equipotent in a radioimmunoassay for human GH. When assessed for growth-promoting activity in the hypophysectomized rat, the isomers were again equipotent with each other and with the GH preparation from which they were derived. The charge isomers also had significant in vitro insulin-like activity on isolated rat adipose tissue and diabetogenic activity in the ob/ob mouse. Thus, the biological activity profiles of these charge isomers of human GH do not differ greatly from one another.

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Characterization of in-vitro drug release and biological activity of methotrexate-bovine serum albumin conjugates

Journal of Pharmacy and Pharmacology ◽

10.1111/j.2042-7158.1987.tb03120.x ◽

1987 ◽

Vol 39 (11) ◽

pp. 871-876 ◽

Cited By ~ 22

Author(s):

G. W. Halbert ◽

A. T. Florence ◽

J. F. B. Stuart

Keyword(s):

Biological Activity ◽

Bovine Serum Albumin ◽

Drug Release ◽

Serum Albumin ◽

Bovine Serum ◽

In Vitro Drug Release

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Characterization of the lipopolysaccharide from Actinobacillus actinomycetemcomitans Y4 and N27

Infection and Immunity ◽

10.1128/iai.30.3.862-873.1980 ◽

1980 ◽

Vol 30 (3) ◽

pp. 862-873

Author(s):

P Kiley ◽

S C Holt

Keyword(s):

Fatty Acids ◽

Biological Activity ◽

Platelet Aggregation ◽

Macrophage Activation ◽

Lipid A ◽

Dry Weight ◽

Actinobacillus Actinomycetemcomitans ◽

Phenol Water

The lipopolysaccharide (LPS) from Actinobacillus actinomycetemcomitans strains Y4 and N27 was isolated by the phenol-water procedure. Morphologically, the molecule consisted of ribbon and branched filaments which comprised 3% of the cellular dry weight. Chemical analysis of the isolated and purified LPSs of both strains showed them to consist of carbohydrate, lipid, 2-keto-3-deoxyoctonate, heptose, hexosamine, and phosphate. The major fatty acids of the lipid A moiety were saturated C14 and beta-OH C14 compounds. Rhamnose, fucose, galactose, glucose, heptose, glucosamine, and galactosamine comprised the monosaccharide portion of the LPS. Biological activity studies revealed both LPS molecules to be active in the Schwartzman reaction and in in vitro 45Ca bone resorption, as well as in macrophage activation and lethality and in platelet aggregation.

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