scholarly journals Monitoring growth of Cellulomonas uda in solid state fermentations with Brewers’ spent grain and optimization of the bioprocess

2021 ◽  
Author(s):  
Alexander Akermann ◽  
Jens Weiermueller ◽  
Jonas Chodorski ◽  
Malte Nestriepke ◽  
Maria Baclig ◽  
...  
Keyword(s):  
Solid State ◽  
Ethanol Concentration ◽  
Scale Up ◽  
Gas Stripping ◽  
Lignocellulosic Feedstock ◽  
Liquid Products ◽  
Brewing Process ◽  
Spent Grain ◽  
Final Ethanol Concentration ◽  
Cellulomonas Uda

Brewers’ spent grain (BSG) is a low-value by-product of the brewing process, which is produced in large quantities every year. In this study, the lignocellulosic feedstock was used to run solid state fermentations with Cellulomonas uda. For aerobic processes, maximum cellulase activities of 0.98 nkat∙ml, maximum xylanase activities of 5.00 nkat∙ml and cell yields of 0.22 g∙g were achieved. Under anaerobic conditions, enzyme activities and cell yields were lower, but valuable liquid products (organic acids, ethanol) were produced with a yield of 0.41 g∙g. The growth phase of the organisms was monitored by measuring extracellular concentrations of two fluorophores pyridoxin (aerobic) and tryptophan (anaerobic) and by cell count. By applying reductive conditions to the anaerobic approach, the ratio of ethanol to acetate was increased from 1.08 to 1.59 mol∙mol. This ratio was further improved to 9.2 mol∙mol by lowering the pH from 7.4 to 5.0 without decreasing the final ethanol concentration. A scale up fermentation with 15w% BSG instead of 5w% BSG quadrupled the acetate concentration, whilst ethanol was removed by gas stripping. This study provides various ideas for optimizing and monitoring solid state fermentations, which can support feasibility and incorporation into holistic biorefining approaches in the future.

scholarly journals Screening a Strain of Klebsiella sp. O852 and the Optimization of Fermentation Conditions for Trans-Dihydrocarvone Production

Molecules ◽  
2021 ◽  
Vol 26 (9) ◽  
pp. 2432
Author(s):  
Li Chen ◽  
Lu-Lu Zhang ◽  
Jing-Nan Ren ◽  
Xiao Li ◽  
Gang Fan ◽  
...  
Keyword(s):  
Ethanol Concentration ◽  
Flavor Compounds ◽  
Accession Number ◽  
Reaction Parameters ◽  
Isolation And Characterization ◽  
Biotransformation Process ◽  
Pharmaceutical Industries ◽  
Final Ethanol Concentration ◽  
Optimization Of Fermentation

Flavors and fragrances have high commercial value in the food, cosmetic, chemical and pharmaceutical industries. It is interesting to investigate the isolation and characterization of new microorganisms with the ability to produce flavor compounds. In this study, a new strain of Klebsiella sp. O852 (accession number CCTCC M2020509) was isolated from decayed navel orange (Citrus sinensis (L.) Osbeck), which was proved to be capable of converting limonene to trans-dihydrocarvone. Besides, the optimization of various reaction parameters to enhance the trans-dihydrocarvone production in shake flask was performed for Klebsiella sp. O852. The results showed that the yield of trans-dihydrocarvone reached up to 1 058 mg/L when Klebsiella sp. O852 was incubated using LB-M medium for 4 h at 36 °C and 150 rpm, and the biotransformation process was monitored for 36 h after adding 1680 mg/L limonene/ethanol (final ethanol concentration of 0.8% (v/v)). The content of trans-dihydrocarvone increased 16 times after optimization. This study provided a basis and reference for producing trans-dihydrocarvone by biotransformation.

scholarly journals Single-step ethanol production from raw cassava starch using a combination of raw starch hydrolysis and fermentation, scale-up from 5-L laboratory and 200-L pilot plant to 3000-L industrial fermenters

2021 ◽  
Vol 14 (1) ◽  
Author(s):  
Morakot Krajang ◽  
Kwanruthai Malairuang ◽  
Jatuporn Sukna ◽  
Krongchan Rattanapradit ◽  
Saethawat Chamsart
Keyword(s):  
Ethanol Production ◽  
Ethanol Concentration ◽  
Consolidated Bioprocessing ◽  
Scale Up ◽  
Cassava Starch ◽  
Single Step ◽  
Starch Hydrolysis ◽  
Yield Coefficient ◽  
Raw Starch ◽  
Raw Starch Hydrolysis

Abstract Background A single-step ethanol production is the combination of raw cassava starch hydrolysis and fermentation. For the development of raw starch consolidated bioprocessing technologies, this research was to investigate the optimum conditions and technical procedures for the production of ethanol from raw cassava starch in a single step. It successfully resulted in high yields and productivities of all the experiments from the laboratory, the pilot, through the industrial scales. Yields of ethanol concentration are comparable with those in the commercial industries that use molasses and hydrolyzed starch as the raw materials. Results Before single-step ethanol production, studies of raw cassava starch hydrolysis by a granular starch hydrolyzing enzyme, StargenTM002, were carefully conducted. It successfully converted 80.19% (w/v) of raw cassava starch to glucose at a concentration of 176.41 g/L with a productivity at 2.45 g/L/h when it was pretreated at 60 °C for 1 h with 0.10% (v/w dry starch basis) of Distillase ASP before hydrolysis. The single-step ethanol production at 34 °C in a 5-L fermenter showed that Saccharomyces cerevisiae (Fali, active dry yeast) produced the maximum ethanol concentration, pmax at 81.86 g/L (10.37% v/v) with a yield coefficient, Yp/s of 0.43 g/g, a productivity or production rate, rp at 1.14 g/L/h and an efficiency, Ef of 75.29%. Scale-up experiments of the single-step ethanol production using this method, from the 5-L fermenter to the 200-L fermenter and further to the 3000-L industrial fermenter were successfully achieved with essentially good results. The values of pmax,Yp/s, rp, and Ef of the 200-L scale were at 80.85 g/L (10.25% v/v), 0.42 g/g, 1.12 g/L/h and 74.40%, respectively, and those of the 3000-L scale were at 70.74 g/L (8.97% v/v), 0.38 g/g, 0.98 g/L/h and 67.56%, respectively. Because of using raw starch, major by-products, i.e., glycerol, lactic acid, and acetic acid of all three scales were very low, in ranges of 0.940–1.140, 0.046–0.052, 0.000–0.059 (% w/v), respectively, where are less than those values in the industries. Conclusion The single-step ethanol production using the combination of raw cassava starch hydrolysis and fermentation of three fermentation scales in this study is practicable and feasible for the scale-up of industrial production of ethanol from raw starch.

scholarly journals Conversion of brewers’ spent grain into proteinaceous animal feed using solid state fermentation

2021 ◽  
Author(s):  
Christos Eliopoulos ◽  
Dimitrios Arapoglou ◽  
Nikos Chorianopoulos ◽  
Giorgos Markou ◽  
Serkos A. Haroutounian
Keyword(s):  
Solid State ◽  
Solid State Fermentation ◽  
Animal Feed ◽  
Spent Grain

scholarly journals Erratum to: Optimization of Xylanase Production by Filamentous Fungi in Solid-State Fermentation and Scale-up to Horizontal Tube Bioreactor

2015 ◽  
Vol 176 (1) ◽  
pp. 317-319 ◽  
Author(s):  
N. Pérez-Rodríguez ◽  
F. Oliveira ◽  
B. Pérez-Bibbins ◽  
I. Belo ◽  
A. Torrado Agrasar ◽  
...  
Keyword(s):  
Solid State ◽  
Filamentous Fungi ◽  
Solid State Fermentation ◽  
Scale Up ◽  
Horizontal Tube ◽  
Xylanase Production

scholarly journals HTC of Wet Residues of the Brewing Process: Comprehensive Characterization of Produced Beer, Spent Grain and Valorized Residues

Energies ◽  
2020 ◽  
Vol 13 (8) ◽  
pp. 2058 ◽  
Author(s):  
Mateusz Jackowski ◽  
Lukasz Niedzwiecki ◽  
Magdalena Lech ◽  
Mateusz Wnukowski ◽  
Amit Arora ◽  
...  
Keyword(s):  
Main Product ◽  
Hydrothermal Carbonization ◽  
Fuel Properties ◽  
Mass Yield ◽  
Tracer Method ◽  
Brewing Process ◽  
Spent Grain ◽  
Potential Use ◽  
Comprehensive Characterization

Steady consumption of beer results in a steady output of residues, i.e., brewer’s spent grain (BSG). Its valorization, using hydrothermal carbonization (HTC) seems sensible. However, a significant knowledge gap regarding the variability of this residue and its influence on the valorization process and its potential use in biorefineries exists. This study attempted to fill this gap by characterization of BSG in conjunction with the main product (beer), taking into accounts details of the brewing process. Moreover, different methods to assess the performance of HTC were investigated. Overall, the differences in terms of the fuel properties of both types of spent grain were much less stark, in comparison to the differences between the respective beers. The use of HTC as a pretreatment of BSG for subsequent use as a biorefinery feedstock can be considered beneficial. HTC was helpful in uniformization and improvement of the fuel properties. A significant decrease in the oxygen content and O/C ratio and improved grindability was achieved. The Weber method proved to be feasible for HTC productivity assessment for commercial installations, giving satisfactory results for most of the cases, contrary to traditional ash tracer method, which resulted in significant overestimations of the mass yield.

Effect of solid-state fermentation over the release of phenolic compounds from brewer's spent grain revealed by UPLC-MSE

LWT ◽  
2020 ◽  
Vol 133 ◽  
pp. 110136 ◽  
Author(s):  
Ingrid da Costa Maia ◽  
Carolina Thomaz dos Santos D'Almeida ◽  
Denise Maria Guimarães Freire ◽  
Elisa d'Avila Costa Cavalcanti ◽  
Luiz Claudio Cameron ◽  
...  
Keyword(s):  
Solid State ◽  
Phenolic Compounds ◽  
Solid State Fermentation ◽  
Brewer’S Spent Grain ◽  
Spent Grain

scholarly journals Ethanol Production from Nondetoxified Dilute-Acid Lignocellulosic Hydrolysate by Cocultures of Saccharomyces cerevisiae Y5 and Pichia stipitis CBS6054

2012 ◽  
Vol 2012 ◽  
pp. 1-6 ◽  
Author(s):  
Ping Wan ◽  
Dongmei Zhai ◽  
Zhen Wang ◽  
Xiushan Yang ◽  
Shen Tian
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Ethanol Production ◽  
Ethanol Concentration ◽  
Ethanol Yield ◽  
Synthetic Medium ◽  
Pichia Stipitis ◽  
Dilute Acid ◽  
Acid Hydrolysate ◽  
Issatchenkia Orientalis ◽  
Final Ethanol Concentration

Saccharomyces cerevisiae Y5 (CGMCC no. 2660) and Issatchenkia orientalis Y4 (CGMCC no. 2159) were combined individually with Pichia stipitis CBS6054 to establish the cocultures of Y5 + CBS6054 and Y4 + CBS6054. The coculture Y5 + CBS6054 effectively metabolized furfural and HMF and converted xylose and glucose mixture to ethanol with ethanol concentration of 16.6 g/L and ethanol yield of 0.46 g ethanol/g sugar, corresponding to 91.2% of the maximal theoretical value in synthetic medium. Accordingly, the nondetoxified dilute-acid hydrolysate was used to produce ethanol by co-culture Y5 + CBS6054. The co-culture consumed glucose along with furfural and HMF completely in 12 h, and all xylose within 96 h, resulting in a final ethanol concentration of 27.4 g/L and ethanol yield of 0.43 g ethanol/g sugar, corresponding to 85.1% of the maximal theoretical value. The results indicated that the co-culture of Y5 + CBS6054 was a satisfying combination for ethanol production from non-detoxified dilute-acid lignocellulosic hydrolysates. This co-culture showed a promising prospect for industrial application.

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