The expression of Muscle ankyrin repeat proteins in brown adipose tissue

MARP family members CARP, Ankrd2 and DARP are expressed in the striated muscle, while DARP protein is also detected in brown adipose tissue (BAT). Taking into account recent findings concerning the common origin of muscle and brown fat, expression of CARP and Ankrd2 in mouse BAT was investigated. We demonstrated Ankrd2 expression in both inactive and thermogenically active BAT, while CARP expression was not detected. Our findings suggest that the expression of Ankrd2 in BAT could be a part of the ?myogenic transcriptional signature?, further supporting the evidence that muscle and brown adipose cells arise from the same myoblastic precursor.

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Electron Microscopic Study on Rat Brown Adipose Tissue. Possible Sites of Glucose Turnover in Rat Brown Adipose Cells as shown by Electron Microscope Radioautography after Administration of Glucose-H3.

Journal of Electron Microscopy ◽

10.1093/oxfordjournals.jmicro.a049559 ◽

1967 ◽

Keyword(s):

Adipose Tissue ◽

Electron Microscope ◽

Brown Adipose Tissue ◽

Microscopic Study ◽

Electron Microscopic Study ◽

Glucose Turnover ◽

Electron Microscopic ◽

Brown Adipose ◽

Adipose Cells

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Thermogenic capacity and brown adipose tissue activity in the common marmoset

Comparative Biochemistry and Physiology Part A Physiology ◽

10.1016/0300-9629(85)91047-3 ◽

1985 ◽

Vol 81 (3) ◽

pp. 683-686 ◽

Cited By ~ 10

Author(s):

Nancy J. Rothwell ◽

Michael J. Stock

Keyword(s):

Adipose Tissue ◽

Brown Adipose Tissue ◽

Common Marmoset ◽

Brown Adipose ◽

The Common ◽

Brown Adipose Tissue Activity ◽

Thermogenic Capacity

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The Fine Structure of Brown Adipose Tissue in the Newborn Mouse and Rat

The Journal of Cell Biology ◽

10.1083/jcb.4.6.685 ◽

1958 ◽

Vol 4 (6) ◽

pp. 685-692 ◽

Cited By ~ 183

Author(s):

Leonard Napolitano ◽

Don Fawcett

Keyword(s):

Adipose Tissue ◽

Fine Structure ◽

Brown Adipose Tissue ◽

Newborn Mouse ◽

Uniform Size ◽

Thin Sections ◽

Additional Information ◽

Brown Adipose ◽

The Rich ◽

Adipose Cells

Interscapular fat from newborn rats and mice was fixed in buffered 1 per cent osmium tetroxide and thin sections of the methacrylate-embedded tissue were studied with the electron microscope. The findings have reaffirmed the epithelioid character of brown adipose tissue, and have provided additional information on the relation of its cells to each other and to the rich capillary bed. For the most part, the earlier description of the fine structure of brown adipose cells by Lever, has been confirmed, but our observations on the mitochondria and their relation to fat droplets have led us to different conclusions concerning the role of these organelles in lipogenesis. Mitochondria were often found to be very closely associated with lipide inclusions, but no actual communication between the two was observed and no evidence was found to support the hypothesis that mitochondria are transformed into lipide droplets. Large dense bodies which showed a highly ordered fine structure suggesting a crystalline protein were seen in the matrix of some mitochondria. The cytoplasm of the adipose cells contained fine granules that seemed to be of two kinds: particles of uniform size (∼150 A) and appreciable density that are believed to be ribonucleoprotein, and granules of lower density and more variable size that are tentatively interpreted as a form of glycogen. The Golgi complex of the adipose cells was small and the endoplasmic reticulum almost entirely absent. The significance of the poor development of these organelles is discussed in relation to current concepts of their function.

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Muscle ankyrin repeat proteins: their role in striated muscle function in health and disease

Critical Reviews in Clinical Laboratory Sciences ◽

10.3109/10408363.2011.643857 ◽

2011 ◽

Vol 48 (5-6) ◽

pp. 269-294 ◽

Cited By ~ 43

Author(s):

Snezana Kojic ◽

Dragica Radojkovic ◽

Georgine Faulkner

Keyword(s):

Muscle Function ◽

Striated Muscle ◽

Ankyrin Repeat ◽

Repeat Proteins ◽

Health And Disease ◽

Ankyrin Repeat Proteins

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Seasonal changes in ultrastructure of brown adipose tissue in the common shrew (Sorex araneus L.)

Cell and Tissue Research ◽

10.1007/bf00233501 ◽

1981 ◽

Vol 214 (3) ◽

pp. 623-632 ◽

Cited By ~ 4

Author(s):

Aleksandra Przełecka

Keyword(s):

Adipose Tissue ◽

Brown Adipose Tissue ◽

Seasonal Changes ◽

Common Shrew ◽

Sorex Araneus ◽

Brown Adipose ◽

The Common ◽

Common Shrew Sorex Araneus

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Cyanidin-3-O-glucoside Regulates the Expression of Ucp1 in Brown Adipose Tissue by Activating Prdm16 Gene

Antioxidants ◽

10.3390/antiox10121986 ◽

2021 ◽

Vol 10 (12) ◽

pp. 1986

Author(s):

Suping Han ◽

Yafan Yang ◽

Yanan Lu ◽

Jielong Guo ◽

Xue Han ◽

...

Keyword(s):

Adipose Tissue ◽

Brown Adipose Tissue ◽

293T Cell ◽

Diet Induced Obesity ◽

High Fructose ◽

Brown Adipose ◽

Prdm16 Gene ◽

Adipose Cells

(1) Background: Brown adipose tissue (BAT) burns energy to produce heat. Cyanidin-3-O-glucoside (C3G) can then enhance the thermogenic ability of BAT in vivo. However, the mechanism by which C3G regulates Ucp1 protein expression remains unclear. (2) Methods: In this study, C3H10T12 brown adipose cells and db/db mice and mice with high-fat, high-fructose, diet-induced obesity were used as the model to explore the effect of C3G on the expression of the Ucp1 gene. Furthermore, the 293T cell line was used for an in vitro cell transgene, a double luciferase reporting system, and yeast single hybridization to explore the mechanism of C3G in regulating Ucp1 protein. (3) Results: we identified that, under the influence of C3G, Prdm16 directly binds to the −500 to −150 bp promoter region of Ucp1 to activate its transcription and, thus, facilitate BAT programming. (4) Conclusions: This study clarified the mechanism by which C3G regulates the expression of the Ucp1 gene of brown fat to a certain extent.

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Functional characterization of human brown adipose tissue metabolism

Biochemical Journal ◽

10.1042/bcj20190464 ◽

2020 ◽

Vol 477 (7) ◽

pp. 1261-1286 ◽

Cited By ~ 3

Author(s):

Marie Anne Richard ◽

Hannah Pallubinsky ◽

Denis P. Blondin

Keyword(s):

Adipose Tissue ◽

Brown Adipose Tissue ◽

Functional Characterization ◽

Human Infants ◽

Positron Emission ◽

Brown Adipose ◽

Treatment Of Obesity ◽

And Function

Brown adipose tissue (BAT) has long been described according to its histological features as a multilocular, lipid-containing tissue, light brown in color, that is also responsive to the cold and found especially in hibernating mammals and human infants. Its presence in both hibernators and human infants, combined with its function as a heat-generating organ, raised many questions about its role in humans. Early characterizations of the tissue in humans focused on its progressive atrophy with age and its apparent importance for cold-exposed workers. However, the use of positron emission tomography (PET) with the glucose tracer [18F]fluorodeoxyglucose ([18F]FDG) made it possible to begin characterizing the possible function of BAT in adult humans, and whether it could play a role in the prevention or treatment of obesity and type 2 diabetes (T2D). This review focuses on the in vivo functional characterization of human BAT, the methodological approaches applied to examine these features and addresses critical gaps that remain in moving the field forward. Specifically, we describe the anatomical and biomolecular features of human BAT, the modalities and applications of non-invasive tools such as PET and magnetic resonance imaging coupled with spectroscopy (MRI/MRS) to study BAT morphology and function in vivo, and finally describe the functional characteristics of human BAT that have only been possible through the development and application of such tools.

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