Electron Microscopic Study on Rat Brown Adipose Tissue. Possible Sites of Glucose Turnover in Rat Brown Adipose Cells as shown by Electron Microscope Radioautography after Administration of Glucose-H3.

1967 ◽  
Keyword(s):  
Adipose Tissue ◽  
Electron Microscope ◽  
Brown Adipose Tissue ◽  
Microscopic Study ◽  
Electron Microscopic Study ◽  
Glucose Turnover ◽  
Electron Microscopic ◽  
Brown Adipose ◽  
Adipose Cells

1. An Improved Method for Processing Early Rat Otocysts for the Electron Microscope

1977 ◽  
Vol 86 (1_suppl) ◽  
pp. 1-8 ◽  
Author(s):  
Trina Schulte ◽  
Khalid M. Khan ◽  
William F. Marovitz
Keyword(s):  
Electron Microscope ◽  
Water Content ◽  
Microscopic Study ◽  
Electron Microscopic Study ◽  
High Water ◽  
Embryonic Tissue ◽  
High Water Content ◽  
Improved Method ◽  
Electron Microscopic ◽  
Specific Fixation

Various methods of dissection, fixation, osmication, sectioning and staining were tested in order to develop an acceptable technique for preparing 9, 10, 11, and 12-day-old rat otocysts for electron microscopic study. The general problems associated with embryonic tissue — Difficult handling, high water content and poor stainability are discussed, and concrete methods of preparation which significantly decrease these difficulties are proposed. The specific fixation and sectioning requirements of rat otocyst are also described in the elaboration of a method which will be used in subsequent studies of the organogenesis of rodent ear.

scholarly journals The expression of Muscle ankyrin repeat proteins in brown adipose tissue

2011 ◽  
Vol 63 (4) ◽  
pp. 915-920
Author(s):  
Ljiljana Rakicevic ◽  
Natasa Petrovic ◽  
Dragica Radojkovic ◽  
Snezana Kojic
Keyword(s):  
Adipose Tissue ◽  
Brown Adipose Tissue ◽  
Striated Muscle ◽  
Ankyrin Repeat ◽  
Transcriptional Signature ◽  
Repeat Proteins ◽  
Brown Adipose ◽  
The Common ◽  
Ankyrin Repeat Proteins ◽  
Adipose Cells

MARP family members CARP, Ankrd2 and DARP are expressed in the striated muscle, while DARP protein is also detected in brown adipose tissue (BAT). Taking into account recent findings concerning the common origin of muscle and brown fat, expression of CARP and Ankrd2 in mouse BAT was investigated. We demonstrated Ankrd2 expression in both inactive and thermogenically active BAT, while CARP expression was not detected. Our findings suggest that the expression of Ankrd2 in BAT could be a part of the ?myogenic transcriptional signature?, further supporting the evidence that muscle and brown adipose cells arise from the same myoblastic precursor.

scholarly journals The Fine Structure of Brown Adipose Tissue in the Newborn Mouse and Rat

1958 ◽  
Vol 4 (6) ◽  
pp. 685-692 ◽  
Author(s):  
Leonard Napolitano ◽  
Don Fawcett
Keyword(s):  
Adipose Tissue ◽  
Fine Structure ◽  
Brown Adipose Tissue ◽  
Newborn Mouse ◽  
Uniform Size ◽  
Thin Sections ◽  
Additional Information ◽  
Brown Adipose ◽  
The Rich ◽  
Adipose Cells

Interscapular fat from newborn rats and mice was fixed in buffered 1 per cent osmium tetroxide and thin sections of the methacrylate-embedded tissue were studied with the electron microscope. The findings have reaffirmed the epithelioid character of brown adipose tissue, and have provided additional information on the relation of its cells to each other and to the rich capillary bed. For the most part, the earlier description of the fine structure of brown adipose cells by Lever, has been confirmed, but our observations on the mitochondria and their relation to fat droplets have led us to different conclusions concerning the role of these organelles in lipogenesis. Mitochondria were often found to be very closely associated with lipide inclusions, but no actual communication between the two was observed and no evidence was found to support the hypothesis that mitochondria are transformed into lipide droplets. Large dense bodies which showed a highly ordered fine structure suggesting a crystalline protein were seen in the matrix of some mitochondria. The cytoplasm of the adipose cells contained fine granules that seemed to be of two kinds: particles of uniform size (∼150 A) and appreciable density that are believed to be ribonucleoprotein, and granules of lower density and more variable size that are tentatively interpreted as a form of glycogen. The Golgi complex of the adipose cells was small and the endoplasmic reticulum almost entirely absent. The significance of the poor development of these organelles is discussed in relation to current concepts of their function.

scholarly journals An Electron Microscopic Study of the Changes in Platelets during Viscous Metamorphosis

Blood ◽  
1962 ◽  
Vol 20 (5) ◽  
pp. 566-580 ◽  
Author(s):  
P. A. CASTALDI ◽  
B. G. FIRKIN ◽  
P. M. BLACKWELL ◽  
K. I. CLIFFORD
Keyword(s):  
Electron Microscope ◽  
Microscopic Study ◽  
Electron Microscopic Study ◽  
Light Microscope ◽  
Phase Contrast ◽  
Intact Cell ◽  
Complete Loss ◽  
Electron Microscopic ◽  
Thin Sections ◽  
Sectioned Material

Abstract Viscous metamorphosis of platelets has been studied with the light microscope, and ultra-thin sections have been prepared at progressive stages for examination in the electron microscope. The phase contrast light microscope reveals rapid aggregation and distortion of platelets and gives the impression of their fusion into structureless aggregates during viscous metamorphosis. Sectioned material collected during viscous metamorphosis of platelets and examined in the electron microscope reveals a remarkable degree of retention of structure in a majority of the platelets. All become deficient in granules and devoid of vesicular spaces, but most retain intact cell membranes, and the structureless masses seen with the light microscope are found to consist of densely aggregated platelets. Fusion and complete loss of identity occurs in the minority. The retracted clot was found to contain densely aggregated, distorted and elongated platelets, empty of granules and intimately related to fibrin particles.

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