scholarly journals Immobilization of glucoamylase on macroporous spheres

2004 ◽  
pp. 207-214 ◽  
Author(s):  
Nenad Milosavic ◽  
Radivoje Prodanovic ◽  
Slobodan Jovanovic ◽  
Zoran Vujcic
Keyword(s):  
Ethylene Glycol ◽  
Coupling Agent ◽  
Glycidyl Methacrylate ◽  
Specific Activity ◽  
Ethylene Glycol Dimethacrylate ◽  
Glycol Dimethacrylate ◽  
Temperature Optimum ◽  
Km Value ◽  
Spacer Arm ◽  
Starch Hydrolysate

Glucoamylase was covalently immobilized through the spacer-arm of the poly(glycidyl methacrylate-co-ethylene glycol dimethacrylate) spheres by using a glutaraldehyde as a coupling agent. The influence of the enzyme load applied to the support on immobilization, yield and specific activity, has been determined. Obtained specific activity was 700 U/g with immobilization yield of 35 %. The Km value for immobilized glucoamylase was 1.28 % (w/v), pH and temperature optimum were 4.5 and 70?C, respectively. The conversion of 20 % (w/w) starch hydrolysate achieved with the immobilized glucoamylase was 97 % after 5 hours.

scholarly journals Stabilization of α-glucosidase in organic solvents by immobilization on macroporous poly(GMA-co-EGDMA) with different surface characteristics

2006 ◽  
Vol 71 (4) ◽  
pp. 339-347 ◽  
Author(s):  
Radivoje Prodanovic ◽  
Nenad Milosavic ◽  
Slobodan Jovanovic ◽  
Tanja Cirkovic-Velickovic ◽  
Zoran Vujcic ◽  
...  
Keyword(s):  
Ethylene Glycol ◽  
Pore Size ◽  
Organic Solvents ◽  
Glycidyl Methacrylate ◽  
Immobilized Enzyme ◽  
Specific Activity ◽  
Surface Characteristics ◽  
Dimethyl Sulphoxide ◽  
Ethylene Glycol Dimethacrylate ◽  
Glycol Dimethacrylate

?-Glucosidase from baker?s yeast was immobilized on macroporous copolymers of ethylene glycol dimethacrylate and glycidyl methacrylate, poly(GMA-co-EGDMA) with various surface characteristics and pore sizes ranging from 44 nm to 270 nm. Immobilization was done by glutaraldehyde on the copolymer previously modified with 1,2-diaminoethane. The specific activity of the obtained immobilized enzyme varied from 27 to 81 U/g, depending on the employed copolymer. The half lives of the immobilized enzyme in cosolvents were influenced by the surface characteristics of the copolymer, ranging from 60 to 150 min in 35% methanol and from 10 to 44 min in 45% dimethyl sulphoxide (DMSO). The best stabilities were obtained when the enzyme was immobilized onto a copolymer having a pore size of 48 nm in methanol and 270 nm in DMSO.

Monodisperse, nanoporous ceria microspheres embedded with Pt nanoparticles: general facile synthesis and catalytic application

RSC Advances ◽  
2014 ◽  
Vol 4 (81) ◽  
pp. 42965-42970 ◽  
Author(s):  
Lin Zhou ◽  
Xiaoxiao Li ◽  
Yong Wang ◽  
Mei Hong ◽  
Yongye Liang ◽  
...  
Keyword(s):  
Ethylene Glycol ◽  
Glycidyl Methacrylate ◽  
Pt Nanoparticles ◽  
Ethylene Glycol Dimethacrylate ◽  
Nanoporous Structure ◽  
Glycol Dimethacrylate ◽  
Hard Template ◽  
Facile Synthesis ◽  
Catalytic Application

A monodisperse Pt/ceria hybrid with controlled nanoporous structure was fabricated with the aid of poly(glycidyl methacrylate-co-ethylene glycol dimethacrylate) microspheres as a hard template.

scholarly journals Synthesis and Application of Histidine-Modified Poly(Glycidyl Methacrylate/Ethylene Glycol Dimethacrylate) Sorbent for Isolation of Caffeine from Black and Green Tea Samples

2018 ◽  
Vol 81 (11) ◽  
pp. 1467-1474 ◽  
Author(s):  
Florian Meischl ◽  
Klemens Losso ◽  
Christian G. Kirchler ◽  
Stefan E. Stuppner ◽  
Christian W. Huck ◽  
...  
Keyword(s):  
Ethylene Glycol ◽  
Green Tea ◽  
Glycidyl Methacrylate ◽  
Ethylene Glycol Dimethacrylate ◽  
Glycol Dimethacrylate

scholarly journals Sorption of different phenol derivatives on functionalized macroporous nanocomposite of poly (glycidyl methacrylate-co-ethylene glycol dimethacrylate) and acid modified bentonite

2014 ◽  
Vol 79 (10) ◽  
pp. 1249-1261 ◽  
Author(s):  
Sanja Marinovic ◽  
Aleksandra Milutinovic-Nikolic ◽  
Aleksandra Nastasovic ◽  
Marija Zunic ◽  
Zorica Vukovic ◽  
...  
Keyword(s):  
Ethylene Glycol ◽  
Glycidyl Methacrylate ◽  
Ethylene Glycol Dimethacrylate ◽  
Order Kinetic ◽  
Glycol Dimethacrylate ◽  
Modified Bentonite ◽  
Phenol Derivatives ◽  
Order Kinetic Model ◽  
Influence Of Temperature On ◽  
Pseudo Second Order

Macroporous nanocomposite of poly (glycidyl methacrylate-co-ethylene glycol dimethacrylate) and acid modified bentonite was prepared by radical suspension copolymerization. Nanocomposite was functionalized with diethylene triamine (deta), by ring-opening reaction of the pendant epoxy groups. Functionalization was performed in order to enable phenol derivatives sorption. This new, not sufficiently investigated material, with developed porous structure was denoted CP-SA-deta. In this study, the influence of temperature on 4-nitrophenol (4NP) sorption on CP-SA-deta was investigated. The chemisorption was estimated as dominant process since activation energy of sorption of 4NP of 54.8 kJ mol-1 was obtained. After determining the optimal sorption conditions for 4NP, the sorption of 2-nitrophenol (2NP) and 2-chloro 4-nitrophenol (2Cl4NP) on CP-SA-deta was investigated with respect to pH, initial concentration and contact time. The 2NP sorption was seldom tested, while according to our knowledge, the 2Cl4NP sorption was not investigated. The isotherm data were best fitted with Langmuir model, while the sorption dynamics obeyed the pseudo-second-order kinetic model for all derivatives.

scholarly journals Glycidyl Methacrylate-ethylene Glycol Dimethacrylate Copolymers with Varied Pore Structures Prepared with Different Reaction Parameters

2020 ◽  
Vol 23 (3) ◽  
Author(s):  
Luciana C. Costa ◽  
Rafaela C. Monteiro ◽  
Helena M. A. Castro ◽  
Tatiane S. Ribeiro ◽  
Mirian A. Oliveira ◽  
...  
Keyword(s):  
Ethylene Glycol ◽  
Glycidyl Methacrylate ◽  
Ethylene Glycol Dimethacrylate ◽  
Glycol Dimethacrylate ◽  
Reaction Parameters ◽  
Pore Structures
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