scholarly journals Genetic biodiversity studies on IGFBP-3 gene in Egyptian sheep breeds

2009 ◽  
Vol 25 (1-2) ◽  
pp. 101-109 ◽  
Author(s):  
B.A. Ali ◽  
A.A. El-Hanafy ◽  
H.H. Salem
Keyword(s):  
Growth And Development ◽  
Genomic Dna ◽  
Dna Polymorphism ◽  
Restriction Pattern ◽  
Exon 2 ◽  
Sheep Breeds ◽  
Pcr Rflp ◽  
Intron 2 ◽  
Igfbp 3 ◽  
Indian Sheep

The insulin-like growth factor binding protein-3 (IGFBP-3) gene is a structural gene associated with the growth and development of the animals. The present investigation was carried out to study DNA polymorphism by PCR-RFLP of IGFBP-3 gene in four Egyptian local sheep breeds and its comparison with Indian sheep breeds. Genomic DNA was isolated from a total of 20 animals from four Egyptian breeds of sheep namely Rahmani, Ossimi, Awassi, and Barki. A fragment of IGFBP-3 gene, comprising of a part of exon 2, complete intron 2, exon 3, and a part of intron 3, was amplified. The amplified fragment was found to be 654 bp in sheep as compared to earlier reports of 651 bp in cattle and 655 bp in buffalo. On digestion of 654 bp with Hae III restriction enzyme yielded single restriction pattern of five fragments of sizes 201, 201, 87, 67, 57 in all the animals belonging to the four Egyptian breeds studied revealing absence of polymorphism in those four Egyptian sheep breeds. On comparison, this fragment was monomorphic in Indian sheep breeds and buffalo ,while it was reported to be polymorphic in cattle.

scholarly journals Comparative analysis of IGFBP-3 gene sequence in Egyptian sheep, cattle, and buffalo

2019 ◽  
Vol 12 (1) ◽  
Author(s):  
Ahmed. A. Saleh ◽  
Amr M. A. Rashad ◽  
Nada. N. A. M. Hassanine ◽  
Mahmoud A. Sharaby ◽  
Yongju Zhao
Keyword(s):  
Comparative Analysis ◽  
Gene Sequence ◽  
Restriction Enzymes ◽  
Restriction Pattern ◽  
Nucleotide Sequencing ◽  
Sequencing Analysis ◽  
Exon 2 ◽  
Pcr Rflp ◽  
Digestion Profile ◽  
Igfbp 3

Abstract Objective A total of 205 animals from four Egyptian livestock species; cattle (n = 18), buffaloes (n = 12), sheep (n = 150) and goats (n = 25) were used in this study to detect polymorphism and perform comparative analysis for IGFBP-3 gene using DNA sequencing and (PCR–RFLP). Results The amplified fragments were found to be of length 654 bp in sheep, 651 bp in cattle and 655 bp in buffalo. For Falahy goats, PCR was performed to amplify a 316 bp fragment from exon 2 of the IGFBP-3 gene. The digestion of 654 bp with HaeIII restriction enzyme yielded a single restriction pattern for goats, while for cattle, 3 genotypes were identified; (AA), (AB), and (BB). Moreover, for buffalo one genotype (AA) only was found with HaeIII and TaqI restriction enzymes, separately. Also, the digestion profile for goats with HaeIII revealed one pattern only. Nucleotide sequencing of the amplified fragments of IGFBP-3 gene in sheep, cattle, buffalo, and goat was submitted to the NCBI GenBank (Accession no. MG738671.1, MG738673.1, MG738674.1, and MG738672.1, respectively). The nucleotide sequencing analysis indicated similarity percentages in IGFBP-3 gene fragments of 88.54, 89.63 and 95.06% between “sheep and cattle”, “sheep and buffalo”, and “cattle and buffalo”, respectively.

scholarly journals Comparative analysis of IGFBP-3 gene sequence in Egyptian sheep, cattle, and buffalo

2019 ◽  
Author(s):  
Ahmed Abdel-kader Saleh ◽  
Amr M.A. Rashad ◽  
Nada. N.A.M. Hassanine ◽  
Mahmoud A. Sharaby ◽  
Yongju Zhao
Keyword(s):  
Comparative Analysis ◽  
Gene Sequence ◽  
Restriction Enzymes ◽  
Restriction Pattern ◽  
Nucleotide Sequencing ◽  
Sequencing Analysis ◽  
Exon 2 ◽  
Pcr Rflp ◽  
Digestion Profile ◽  
Igfbp 3

Abstract Objective: A total of 205 animals from four Egyptian livestock species; cattle (n=18), buffaloes (n=12), sheep (n=150) and goats (n=25) were used in this study to detect polymorphism and perform comparative analysis for IGFBP-3 gene using DNA sequencing and (PCR-RFLP). Results: The amplified fragments were found to be of length 654 bp in sheep, 651bp in cattle and 655 bp in buffalo. For Falahy goats, PCR was performed to amplify a 316 bp fragment from exon 2 of the IGFBP-3 gene. The digestion of 654 bp with HaeIII restriction enzyme yielded a single restriction pattern for goats, while for cattle, 3 genotypes were identified; (AA),(AB), and (BB). Moreover, for buffalo one genotype (AA) only was found with HaeIII and TaqI restriction enzymes, separately. Also, the digestion profile for goats with HaeIII revealed one pattern only. Nucleotide sequencing of the amplified fragments of IGFBP-3 gene in sheep, cattle, buffalo, and goat was submitted to the NCBI GenBank (Accession no. MG738671.1, MG738673.1, MG738674.1, and MG738672.1, respectively). The nucleotide sequencing analysis indicated similarity percentages in IGFBP-3 gene fragments of 88.54, 89.63 and 95.06 % between ''sheep and cattle '', ''sheep and buffalo '', and ''cattle and buffalo'', respectively.

scholarly journals Comparative analysis of IGFBP-3 gene sequence in Egyptian sheep, cattle, and buffalo

2019 ◽  
Author(s):  
Ahmed Abdel-kader Saleh ◽  
Amr M.A. Rashad ◽  
Nada. N.A.M. Hassanine ◽  
Mahmoud A. Sharaby ◽  
Yongju Zhao
Keyword(s):  
Comparative Analysis ◽  
Gene Sequence ◽  
Restriction Enzymes ◽  
Restriction Pattern ◽  
Nucleotide Sequencing ◽  
Sequencing Analysis ◽  
Exon 2 ◽  
Pcr Rflp ◽  
Digestion Profile ◽  
Igfbp 3

Abstract Objective: A total of 205 animals from four Egyptian livestock species; cattle (n=18), buffaloes (n=12), sheep (n=150) and goats (n=25) were used in this study to detect polymorphism and perform comparative analysis for IGFBP-3 gene using DNA sequencing and (PCR-RFLP). Results: The amplified fragments were found to be of length 654 bp in sheep, 651bp in cattle and 655 bp in buffalo. For Falahy goats, PCR was performed to amplify a 316 bp fragment from exon 2 of the IGFBP-3 gene. The digestion of 654 bp with HaeIII restriction enzyme yielded a single restriction pattern for goats, while for cattle, 3 genotypes were identified; (AA),(AB), and (BB). Moreover, for buffalo one genotype (AA) only was found with HaeIII and TaqI restriction enzymes, separately. Also, the digestion profile for goats with HaeIII revealed one pattern only. Nucleotide sequencing of the amplified fragments of IGFBP-3 gene in sheep, cattle, buffalo, and goat was submitted to the NCBI GenBank (Accession no. MG738671.1, MG738673.1, MG738674.1, and MG738672.1, respectively). The nucleotide sequencing analysis indicated similarity percentages in IGFBP-3 gene fragments of 88.54, 89.63 and 95.06 % between ''sheep and cattle '', ''sheep and buffalo '', and ''cattle and buffalo'', respectively.

scholarly journals Comparative analysis of IGFBP-3 gene sequence in Egyptian sheep, cattle, and buffalo

2019 ◽  
Author(s):  
Ahmed Abdel-kader Saleh ◽  
Amr M.A. Rashad ◽  
Nada. N.A.M. Hassanine ◽  
Mahmoud A. Sharaby ◽  
Yongju Zhao
Keyword(s):  
Comparative Analysis ◽  
Gene Sequence ◽  
Restriction Enzymes ◽  
Restriction Pattern ◽  
Nucleotide Sequencing ◽  
Sequencing Analysis ◽  
Exon 2 ◽  
Pcr Rflp ◽  
Digestion Profile ◽  
Igfbp 3

Abstract Objective: A total of 205 animals from four Egyptian livestock species; cattle (n=18), buffaloes (n=12), sheep (n=150) and goats (n=25) were used in this study to detect polymorphism and perform comparative analysis for IGFBP-3 gene using DNA sequencing and (PCR-RFLP). Results: The amplified fragments were found to be of length 654 bp in sheep, 651bp in cattle and 655 bp in buffalo. For Falahy goats, PCR was performed to amplify a 316 bp fragment from exon 2 of the IGFBP-3 gene. The digestion of 654 bp with HaeIII restriction enzyme yielded a single restriction pattern for goats, while for cattle, 3 genotypes were identified; (AA),(AB), and (BB). Moreover, for buffalo one genotype (AA) only was found with HaeIII and TaqI restriction enzymes, separately. Also, the digestion profile for goats with HaeIII revealed one pattern only. Nucleotide sequencing of the amplified fragments of IGFBP-3 gene in sheep, cattle, buffalo, and goat was submitted to the NCBI GenBank (Accession no. MG738671.1, MG738673.1, MG738674.1, and MG738672.1, respectively). The nucleotide sequencing analysis indicated similarity percentages in IGFBP-3 gene fragments of 88.54, 89.63 and 95.06 % between ''sheep and cattle '', ''sheep and buffalo '', and ''cattle and buffalo'', respectively.

scholarly journals Biomarkers of Adiponectin: Plasma Protein Variation and Genomic DNA Polymorphisms

2009 ◽  
Vol 4 ◽  
pp. BMI.S3453 ◽  
Author(s):  
Harvest F. Gu
Keyword(s):  
Plasma Protein ◽  
Promoter Region ◽  
Genomic Dna ◽  
Dna Polymorphisms ◽  
Promoter Polymorphisms ◽  
Exon 2 ◽  
Plasma Adiponectin ◽  
Adipoq Gene ◽  
Protein Variation ◽  
Intron 2

Adiponectin is secreted by white adipose tissue and exists as the most abundant adipokine in the human plasma. Recent research has indicated that plasma adiponectin levels are inversely correlated with body mass index (BMI) and insulin resistance. Reduction of plasma adiponectin levels is commonly observed in the patients with type 2 diabetes (T2D) and/or in those who are obese in comparison with healthy control individuals. The adiponectin ( AdipoQ) gene has a moderate linkage disequilibrium (LD), but two small LD blocks are observed, respectively, in the promoter region and the boundary of exon 2-intron 2. Genetic association studies have demonstrated that single nucleotide polymorphisms (SNPs) +45G15G(T/G) in exon 2 and +276G/T in intron 2 of the AdipoQ gene confer the risk susceptibility to the development of T2D, obesity and diabetic nephropathy (DN). The SNPs in the promoter region, including –11426A/G, –11377C/G and –11391G/A, are found to be associated with T2Dand DN. Recent research has indicated that the promoter polymorphisms interfere with the AdipoQ promoter activity. The haplotypes constructed by the promoter polymorphisms and SNP +276G/T in intron 2 are associated with circulating adiponectin levels. This review summarises genetic and pathophysiological relevancies of adiponectin and discusses about the biomarkers of adiponectin plasma protein variation and genomic DNA polymorphisms.

scholarly journals Fingerprinting of fecB gene in five Egyptian sheep breeds

2009 ◽  
Vol 25 (3-4) ◽  
pp. 205-212 ◽  
Author(s):  
Hanafy el ◽  
Saadani el
Keyword(s):  
Point Mutation ◽  
Restriction Enzyme ◽  
Base Pair ◽  
Genomic Dna ◽  
Restriction Site ◽  
Wild Type ◽  
Specific Primer ◽  
Sheep Breeds ◽  
Pcr Rflp ◽  
Pcr Products

Recently, many aspects of FecB gene, including reproductive endocrinology, organs development and body mass have been studied. FecB has an additive effect on litter size and ovulation. The present investigation was carried out to study polymorphism by forced PCR-RFLP of FecB gene in five Egyptian local sheep breeds and its comparison with other foreign sheep breeds. Genomic DNA was isolated from a total of 100 animals of Egyptian sheep breeds namely Rahmani, Ossimi, Awassi, Barki and Awassi x Barki crossbred. Forced PCR of the FecB gene 190 base pair (bp) was amplified using specific primer designed to introduc a point mutation in the resulting PCR products with FecB carrier sheep containing an AvaII restriction site (G|GACC), whereas products from noncarriers lacked (of) this site. Digestion of FecB gene 190 base pair with AvaII restriction enzyme resulted in non carrier 190 bp band (wild type) in all the animals belonging to the five Egyptian breeds studied revealing absence of this restriction site in those five breeds. .

scholarly journals Comparative analysis of IGFBP-3 gene sequence in Egyptian sheep, cattle and buffalo

2019 ◽  
Author(s):  
Ahmed Abdel-kader Saleh ◽  
Amr M.A. Rashad ◽  
Nada. N.A.M. Hassanine ◽  
Mahmoud A. Sharabi ◽  
Yongju Zhao
Keyword(s):  
Comparative Analysis ◽  
Gene Sequence ◽  
Restriction Enzymes ◽  
Restriction Pattern ◽  
Nucleotide Sequencing ◽  
Sequencing Analysis ◽  
Livestock Species ◽  
Pcr Rflp ◽  
Digestion Profile ◽  
Igfbp 3

Abstract Objective: A total of 205 animals from four Egyptian livestock species were used in this study to detect polymorphism and perform comparative analysis for IGFBP-3 gene using DNA sequencing and (PCR-RFLP). Results: The digestion of 654 bp with HaeIII restriction enzyme yielded a single restriction pattern of eight fragments in sheep revealing the absence of polymorphism, while in cattle 3 genotypes were identified; (AA), (AB), and (BB). Moreover, one genotype (AA) only was found in buffalo using HeaIII and MspI restriction enzymes, separately. Digestion profile for a goat with HaeIII revealed only one pattern for three DNA fragments, which means the absence of polymorphism within the IGFBP-3 gene in the tested goats. The nucleotide sequencing analysis indicated similarity percentages in IGFBP-3 gene fragments of 77, 30 and 58% between ''sheep and cattle '', ''sheep and buffalo '', and ''cattle and buffalo'', respectively.

Polymorphism in exon 2 of the BuLA-DRB3 gene in Indian buffalo (Bubalus bubalis var. indicus) detected by PCR-RFLP

2000 ◽  
Vol 70 (2) ◽  
pp. 221-226 ◽  
Author(s):  
T.V. Aravindakshan ◽  
A.M. Nainar ◽  
S.N. Sivaselvam
Keyword(s):  
Pcr Primers ◽  
Bubalus Bubalis ◽  
Restriction Pattern ◽  
Fragment Analysis ◽  
Exon 2 ◽  
Pcr Rflp ◽  
Restriction Sites ◽  
Bovine Lymphocyte ◽  
High Degree ◽  
Bovine Species

AbstractPolymerase chain reaction (PCR) primers specific to exon 2 of the bovine lymphocyte antigen (BoLA)-DRB3 gene were used successfully to amplify the equivalent region in 34 Murrah and 36 Surti buffaloes selected at random. The 304 bp amplified product of the DRB3 gene was separately digested with BstγI, HaeIII and Rsal enzymes. Digestion with BstγI enzyme did not reveal any polymorphism and all animals showed a single restriction pattern, which corresponded exactly to the BstγI pattern ‘b’ previously described for cattle. Digestion with HaeIII enzyme resulted in five patterns, four of which corresponded to the Haelll patterns previously reported in cattle. The new HaeIII pattern was observed in both the breeds of buffaloes studied. The fragment analysis with RsaI revealed 13 different patterns. All of these RsaI patterns corresponded to the RsaI patterns previously described for cattle. The high degree of similarity in the restriction fragment length polymorphism (RFLP) patterns of cattle and buffalo observed in the present study provide evidence for the strong conservation amongst other bovine species, of restriction sites previously reported in cattle.

Estudio del polimorfismo y poligenismo de los loci DRB en caballos criollo argentino

2003 ◽  
Author(s):  
◽  
Silvina Díaz
Keyword(s):  
Exon 2 ◽  
Pcr Rflp

El objetivo del trabajo de Tesis Doctoral consistió en estudiar el polimorfismo y el poligenismo de los genes de clase II DRB del Complejo Principal de Histocompatibilidad en Equinos (ELA). La variabilidad genética de las regiones promotoras y de la región de reconocimiento del antígeno (exón 2) se analizó mediante los métodos de PCR-RFLP y PCR-SSCP. Se detectaron tres nuevos alelos del exón 2 definidos por PCR-RFLP, los que se confirmaron por clonado y secuenciación de los productos de amplificación. Además, el número de variantes detectadas en cada animal permitió inferir la presencia de al menos tres copias de genes DRB en los haplotipos equinos analizados. Estos datos se confirmaron a través de análisis filogenético y de segregación. El clonado y secuenciación de la región reguladora (URR) de los genes DRB permitió caracterizar la organización del promotor proximal. Los resultados obtenidos mostraron la presencia en dirección 5´ - 3´ de las cajas conservadas W, X, Y, CCAAT y TATA. Esta estructura es semejante a la reportada en los genes ortólogos de otras especies de mamíferos y evidenciaría que la región analizada corresponde a un gen DRB funcional. Por otra parte, el análisis del polimorfismo del promotor permitió identificar cinco alelos definidos por SSCP. El conjunto de resultados obtenidos mostró que los genes ELA-DRB presentan las principales características descriptas para los genes de Clase II de mamíferos: existencia de copias múltiples, alto grado de polimorfismo, alta tasa de sustituciones no sinónimas en los sitios de reconocimiento del antígeno, y conservación de secuencia y estructura del promotor proximal.

scholarly journals 5′ Region Large Genomic Rearrangements in the BRCA1 Gene in French Families: Identification of a Tandem Triplication and Nine Distinct Deletions with Five Recurrent Breakpoints

Cancers ◽  
2021 ◽  
Vol 13 (13) ◽  
pp. 3171
Author(s):  
Sandrine M. Caputo ◽  
Dominique Telly ◽  
Adrien Briaux ◽  
Julie Sesen ◽  
Maurizio Ceppi ◽  
...  
Keyword(s):  
Brca1 Gene ◽  
Genomic Rearrangements ◽  
Comparative Genomic ◽  
Homologous Region ◽  
Exon 2 ◽  
Large Genomic Rearrangements ◽  
Causality Score ◽  
Frequent Site ◽  
First Time ◽  
Intron 2

Background: Large genomic rearrangements (LGR) in BRCA1 consisting of deletions/duplications of one or several exons have been found throughout the gene with a large proportion occurring in the 5′ region from the promoter to exon 2. The aim of this study was to better characterize those LGR in French high-risk breast/ovarian cancer families. Methods: DNA from 20 families with one apparent duplication and nine deletions was analyzed with a dedicated comparative genomic hybridization (CGH) array, high-resolution BRCA1 Genomic Morse Codes analysis and Sanger sequencing. Results: The apparent duplication was in fact a tandem triplication of exons 1 and 2 and part of intron 2 of BRCA1, fully characterized here for the first time. We calculated a causality score with the multifactorial model from data obtained from six families, classifying this variant as benign. Among the nine deletions detected in this region, eight have never been identified. The breakpoints fell in six recurrent regions and could confirm some specific conformation of the chromatin. Conclusions: Taken together, our results firmly establish that the BRCA1 5′ region is a frequent site of different LGRs and highlight the importance of the segmental duplication and Alu sequences, particularly the very high homologous region, in the mechanism of a recombination event. This also confirmed that those events are not systematically deleterious.

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