Comparative analysis of IGFBP-3 gene sequence in Egyptian sheep, cattle and buffalo

Abstract Objective A total of 205 animals from four Egyptian livestock species; cattle (n = 18), buffaloes (n = 12), sheep (n = 150) and goats (n = 25) were used in this study to detect polymorphism and perform comparative analysis for IGFBP-3 gene using DNA sequencing and (PCR–RFLP). Results The amplified fragments were found to be of length 654 bp in sheep, 651 bp in cattle and 655 bp in buffalo. For Falahy goats, PCR was performed to amplify a 316 bp fragment from exon 2 of the IGFBP-3 gene. The digestion of 654 bp with HaeIII restriction enzyme yielded a single restriction pattern for goats, while for cattle, 3 genotypes were identified; (AA), (AB), and (BB). Moreover, for buffalo one genotype (AA) only was found with HaeIII and TaqI restriction enzymes, separately. Also, the digestion profile for goats with HaeIII revealed one pattern only. Nucleotide sequencing of the amplified fragments of IGFBP-3 gene in sheep, cattle, buffalo, and goat was submitted to the NCBI GenBank (Accession no. MG738671.1, MG738673.1, MG738674.1, and MG738672.1, respectively). The nucleotide sequencing analysis indicated similarity percentages in IGFBP-3 gene fragments of 88.54, 89.63 and 95.06% between “sheep and cattle”, “sheep and buffalo”, and “cattle and buffalo”, respectively.

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Comparative analysis of IGFBP-3 gene sequence in Egyptian sheep, cattle, and buffalo

10.21203/rs.2.13545/v3 ◽

2019 ◽

Author(s):

Ahmed Abdel-kader Saleh ◽

Amr M.A. Rashad ◽

Nada. N.A.M. Hassanine ◽

Mahmoud A. Sharaby ◽

Yongju Zhao

Keyword(s):

Comparative Analysis ◽

Gene Sequence ◽

Restriction Enzymes ◽

Restriction Pattern ◽

Nucleotide Sequencing ◽

Sequencing Analysis ◽

Exon 2 ◽

Pcr Rflp ◽

Digestion Profile ◽

Igfbp 3

Abstract Objective: A total of 205 animals from four Egyptian livestock species; cattle (n=18), buffaloes (n=12), sheep (n=150) and goats (n=25) were used in this study to detect polymorphism and perform comparative analysis for IGFBP-3 gene using DNA sequencing and (PCR-RFLP). Results: The amplified fragments were found to be of length 654 bp in sheep, 651bp in cattle and 655 bp in buffalo. For Falahy goats, PCR was performed to amplify a 316 bp fragment from exon 2 of the IGFBP-3 gene. The digestion of 654 bp with HaeIII restriction enzyme yielded a single restriction pattern for goats, while for cattle, 3 genotypes were identified; (AA),(AB), and (BB). Moreover, for buffalo one genotype (AA) only was found with HaeIII and TaqI restriction enzymes, separately. Also, the digestion profile for goats with HaeIII revealed one pattern only. Nucleotide sequencing of the amplified fragments of IGFBP-3 gene in sheep, cattle, buffalo, and goat was submitted to the NCBI GenBank (Accession no. MG738671.1, MG738673.1, MG738674.1, and MG738672.1, respectively). The nucleotide sequencing analysis indicated similarity percentages in IGFBP-3 gene fragments of 88.54, 89.63 and 95.06 % between ''sheep and cattle '', ''sheep and buffalo '', and ''cattle and buffalo'', respectively.

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Comparative analysis of IGFBP-3 gene sequence in Egyptian sheep, cattle, and buffalo

10.21203/rs.2.13545/v2 ◽

2019 ◽

Author(s):

Ahmed Abdel-kader Saleh ◽

Amr M.A. Rashad ◽

Nada. N.A.M. Hassanine ◽

Mahmoud A. Sharaby ◽

Yongju Zhao

Keyword(s):

Comparative Analysis ◽

Gene Sequence ◽

Restriction Enzymes ◽

Restriction Pattern ◽

Nucleotide Sequencing ◽

Sequencing Analysis ◽

Exon 2 ◽

Pcr Rflp ◽

Digestion Profile ◽

Igfbp 3

Abstract Objective: A total of 205 animals from four Egyptian livestock species; cattle (n=18), buffaloes (n=12), sheep (n=150) and goats (n=25) were used in this study to detect polymorphism and perform comparative analysis for IGFBP-3 gene using DNA sequencing and (PCR-RFLP). Results: The amplified fragments were found to be of length 654 bp in sheep, 651bp in cattle and 655 bp in buffalo. For Falahy goats, PCR was performed to amplify a 316 bp fragment from exon 2 of the IGFBP-3 gene. The digestion of 654 bp with HaeIII restriction enzyme yielded a single restriction pattern for goats, while for cattle, 3 genotypes were identified; (AA),(AB), and (BB). Moreover, for buffalo one genotype (AA) only was found with HaeIII and TaqI restriction enzymes, separately. Also, the digestion profile for goats with HaeIII revealed one pattern only. Nucleotide sequencing of the amplified fragments of IGFBP-3 gene in sheep, cattle, buffalo, and goat was submitted to the NCBI GenBank (Accession no. MG738671.1, MG738673.1, MG738674.1, and MG738672.1, respectively). The nucleotide sequencing analysis indicated similarity percentages in IGFBP-3 gene fragments of 88.54, 89.63 and 95.06 % between ''sheep and cattle '', ''sheep and buffalo '', and ''cattle and buffalo'', respectively.

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Comparative analysis of IGFBP-3 gene sequence in Egyptian sheep, cattle, and buffalo

10.21203/rs.2.13545/v4 ◽

2019 ◽

Author(s):

Ahmed Abdel-kader Saleh ◽

Amr M.A. Rashad ◽

Nada. N.A.M. Hassanine ◽

Mahmoud A. Sharaby ◽

Yongju Zhao

Keyword(s):

Comparative Analysis ◽

Gene Sequence ◽

Restriction Enzymes ◽

Restriction Pattern ◽

Nucleotide Sequencing ◽

Sequencing Analysis ◽

Exon 2 ◽

Pcr Rflp ◽

Digestion Profile ◽

Igfbp 3

Abstract Objective: A total of 205 animals from four Egyptian livestock species; cattle (n=18), buffaloes (n=12), sheep (n=150) and goats (n=25) were used in this study to detect polymorphism and perform comparative analysis for IGFBP-3 gene using DNA sequencing and (PCR-RFLP). Results: The amplified fragments were found to be of length 654 bp in sheep, 651bp in cattle and 655 bp in buffalo. For Falahy goats, PCR was performed to amplify a 316 bp fragment from exon 2 of the IGFBP-3 gene. The digestion of 654 bp with HaeIII restriction enzyme yielded a single restriction pattern for goats, while for cattle, 3 genotypes were identified; (AA),(AB), and (BB). Moreover, for buffalo one genotype (AA) only was found with HaeIII and TaqI restriction enzymes, separately. Also, the digestion profile for goats with HaeIII revealed one pattern only. Nucleotide sequencing of the amplified fragments of IGFBP-3 gene in sheep, cattle, buffalo, and goat was submitted to the NCBI GenBank (Accession no. MG738671.1, MG738673.1, MG738674.1, and MG738672.1, respectively). The nucleotide sequencing analysis indicated similarity percentages in IGFBP-3 gene fragments of 88.54, 89.63 and 95.06 % between ''sheep and cattle '', ''sheep and buffalo '', and ''cattle and buffalo'', respectively.

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Genetic biodiversity studies on IGFBP-3 gene in Egyptian sheep breeds

Biotechnology in Animal Husbandry ◽

10.2298/bah0902101a ◽

2009 ◽

Vol 25 (1-2) ◽

pp. 101-109 ◽

Cited By ~ 6

Author(s):

B.A. Ali ◽

A.A. El-Hanafy ◽

H.H. Salem

Keyword(s):

Growth And Development ◽

Genomic Dna ◽

Dna Polymorphism ◽

Restriction Pattern ◽

Exon 2 ◽

Sheep Breeds ◽

Pcr Rflp ◽

Intron 2 ◽

Igfbp 3 ◽

Indian Sheep

The insulin-like growth factor binding protein-3 (IGFBP-3) gene is a structural gene associated with the growth and development of the animals. The present investigation was carried out to study DNA polymorphism by PCR-RFLP of IGFBP-3 gene in four Egyptian local sheep breeds and its comparison with Indian sheep breeds. Genomic DNA was isolated from a total of 20 animals from four Egyptian breeds of sheep namely Rahmani, Ossimi, Awassi, and Barki. A fragment of IGFBP-3 gene, comprising of a part of exon 2, complete intron 2, exon 3, and a part of intron 3, was amplified. The amplified fragment was found to be 654 bp in sheep as compared to earlier reports of 651 bp in cattle and 655 bp in buffalo. On digestion of 654 bp with Hae III restriction enzyme yielded single restriction pattern of five fragments of sizes 201, 201, 87, 67, 57 in all the animals belonging to the four Egyptian breeds studied revealing absence of polymorphism in those four Egyptian sheep breeds. On comparison, this fragment was monomorphic in Indian sheep breeds and buffalo ,while it was reported to be polymorphic in cattle.

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Sequencing Analysis and Phylogenetic Tree of HPV Isolated from Breast Cancer Patients at Thi-Qar Province/Iraq

10.32792/utq/utjsci/vol7/2/10 ◽

2020 ◽

pp. 37-40

Keyword(s):

Nucleotide Sequence ◽

Phylogenetic Tree ◽

Evolutionary Relationship ◽

Nucleotide Sequencing ◽

Sequencing Analysis ◽

Breast Cancer Patients ◽

The North ◽

Formalin Fixed Paraffin ◽

Formalin Fixed Paraffin Embedded ◽

History Of

Genetic variety examination has demonstrated fundamental to the understanding of the epidemiological and developmental history of Papillomavirus (HPV), for the development of accurate diagnostic tests and for efficient vaccine design. The HPV nucleotide diversity has been investigated widely among high-risk HPV types. To make the nucleotide sequence of HPV and do the virus database in Thi-Qar province, and compare sequences of our isolates with previously described isolates from around the world and then draw its phylogenetic tree, this study done. A total of 6 breast formalin-fixed paraffin-embedded (FFPE) of the female patients were included in the study, divided as 4 FFPE malignant tumor and 2 FFPE of benign tumor. The PCR technique was implemented to detect the presence of HPV in breast tissue, and the real-time PCR used to determinant HPV genotypes, then determined a complete nucleotide sequence of HPV of L1 capsid gene, and draw its phylogenetic tree. The nucleotide sequencing finding detects a number of substitution mutation (SNPs) in (L1) gene, which have not been designated before, were identified once in this study population, and revealed that the HPV16 strains have the evolutionary relationship with the South African race, while, the HPV33 and HPV6 showing the evolutionary association with the North American and East Asian race, respectively.

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Detection of polymorphisms within the human IL10 receptor cDNA gene sequence by RT-PCR RFLP

Immunogenetics ◽

10.1007/s002510050301 ◽

1997 ◽

Vol 46 (5) ◽

pp. 439-441 ◽

Cited By ~ 7

Author(s):

Yosuke Tanaka ◽

H. Nakashima ◽

Takeshi Otsuka ◽

Yoshiaki Nemoto ◽

Hiroaki Niiro ◽

...

Keyword(s):

Gene Sequence ◽

Rt Pcr ◽

Pcr Rflp ◽

Receptor Cdna ◽

Human Il10

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Nucleotide Sequencing Analysis of the 146-Kilobase Segment around theIkBLandMICAGenes at the Centromeric End of the HLA Class I Region

Genomics ◽

10.1006/geno.1997.5114 ◽

1998 ◽

Vol 47 (3) ◽

pp. 372-382 ◽

Cited By ~ 55

Author(s):

Takashi Shiina ◽

Gen Tamiya ◽

Akira Oka ◽

Tetsushi Yamagata ◽

Naomi Yamagata ◽

...

Keyword(s):

Hla Class I ◽

Class I ◽

Nucleotide Sequencing ◽

Sequencing Analysis ◽

Class I Region

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Human c-fms proto-oncogene: comparative analysis with an abnormal allele

Molecular and Cellular Biology ◽

10.1128/mcb.5.2.422-426.1985 ◽

1985 ◽

Vol 5 (2) ◽

pp. 422-426

Author(s):

J S Verbeek ◽

A J Roebroek ◽

A M van den Ouweland ◽

H P Bloemers ◽

W J Van de Ven

Keyword(s):

Comparative Analysis ◽

Dna Sequencing ◽

Sequencing Analysis ◽

Base Pairs ◽

Small Deletion ◽

Viral Oncogene ◽

Close Proximity ◽

Genetic Sequences ◽

Dna Sequencing Analysis

The organization of the human c-fms proto-oncogene has been determined and compared with an abnormal allele. The human v-fms homologous genetic sequences are dispersed discontinuously and colinearly with the viral oncogene over a DNA region of ca. 32 kilobase pairs. The abnormal c-fms locus contains a small deletion in its 3' portion. DNA sequencing analysis indicated that it was 426 base pairs in size and located in close proximity to a putative c-fms exon.

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