scholarly journals Effect of mutagen-ethyl methane sulphonate on yield increasing parameters of French bean (Phaseolus vulgarisl.)

Genetika ◽  
2018 ◽  
Vol 50 (1) ◽  
pp. 199-207
Author(s):  
R Ramandeep ◽  
T.S. Dhillon ◽  
R.K. Dhall ◽  
B.S. Gill
Keyword(s):  
Total Yield ◽  
Bean Common Mosaic Virus ◽  
Germination Percentage ◽  
French Bean ◽  
Ethyl Methane Sulphonate ◽  
Ethyl Methane ◽  
Yield And Quality ◽  
Methane Sulphonate ◽  
Field And Laboratory Conditions ◽  
Pod Length

The present investigation was undertaken at the Vegetable Research Farm, Department of Vegetable Science, Punjab Agricultural University, Ludhiana, INDIA during spring season of 2015 and 2016 with objective to induce genetic variability for yield and quality traits in cultivars ?Contender? and ?Arka Komal? through chemical mutagen- ethyl methane sulphonate (EMS) at 0.15, 0.25 and 0.35%. Seed treatment with all the EMS concentration drastically reduced its germination but the germination per cent decreased with increase in concentration of EMS. The per cent seed germination was maximum (90%) in control ?Contender? whereas it was 74%, 49% and 42% in EMS at 0.15%, 0.25% and 0.35%, respectively. Similar trend of results for germination percentage were obtained in cultivar ?Arka Komal? after treatment with different concentrations of EMS, however germination percentage of ?Arka Komal? was lower than of ?Contender?. The germination percentage trends of EMS treated French bean cultivars were comparable under field and laboratory conditions but somewhat less under field due to less favourable conditions. All the plants harvested in M1 generation in both cultivars were sown in plant to progeny fashion along with parents. Several desirable plants were selected in M2 generation for early flowering, number of pods per plant, pod length and bean common mosaic virus (BCMV) resistance in both the cultivars at different concentrations of EMS. Selection was also performed for straight pods in case of genotype 'Contender'. The maximum frequency of desirable mutants was observed in lower EMS concentration (0.15%). The 0.15% EMS increased number of pods per plant which may be directly related to yield per plant and it will ultimately increase the total yield. Therefore, EMS concentration at 0.15% can be effectively used for inducing maximum variability in French bean. The mutant population developed in M2 generation can be further used to check its performance in next generations.

scholarly journals Efficacy EMS and DES on Mutagenesis and seedling characters of Eleusine coracana L. Gaertn.

1970 ◽  
pp. 09-11
Author(s):  
L. Mullainathan, K. Aviya
Keyword(s):  
Seed Germination ◽  
Finger Millet ◽  
Germination Percentage ◽  
Eleusine Coracana ◽  
Ethyl Methane Sulphonate ◽  
Ethyl Methane ◽  
Biological Damage ◽  
Methane Sulphonate ◽  
Ld50 Value ◽  
Seedling Characters

This study was performed by exposing the seeds of Finger millet (Eleusine coracana L. Gaertn.) Var CO -13 to Ethyl Methane Sulphonate (EMS) and Diethyl sulphate (DES). The observations were made on seed germination and survivability in M1 generation. The study revealed that germination percentage and survivability were decreased by increasing concentration of the mutagens when compared to the control. The LD50 value was found in 30mM of EMS and 40mM of DES. So it was found that both mutagens at lower treatments have influenced less biological damage and could be suitable for inducing desirable mutations in Finger millet.

Evaluation of Yield Attributing Variants Developed Through Ethyl Methane Sulphonate in an Important Proteinaceous Crop-Vicia faba

2017 ◽  
Vol 9 (2) ◽  
pp. 20-27 ◽  
Author(s):  
Durre Shahwar ◽  
Mohammad Yunus Khali Ansari ◽  
Sana Chaudhary ◽  
Rumana Aslam
Keyword(s):  
Vicia Faba ◽  
Ethyl Methane Sulphonate ◽  
Ethyl Methane ◽  
Methane Sulphonate

Effect of seed treatment by ethyl methane sulphonate on growth, flowering and yield of papaya cv. Pusa Dwarf

2016 ◽  
Vol 7 (1) ◽  
pp. 64
Author(s):  
Mahesh Kumar ◽  
Mukesh Kumar ◽  
Satya Prakash ◽  
DK Gautam ◽  
Sanjeev Rao
Keyword(s):  
Seed Treatment ◽  
Ethyl Methane Sulphonate ◽  
Ethyl Methane ◽  
Methane Sulphonate

scholarly journals Increased sensitivity to cell killing and mutagenesis by chemical mutagens in thymidine-kinase-deficient subclones of a Friend murine leukaemia cell line

1982 ◽  
Vol 40 (2) ◽  
pp. 207-212 ◽  
Author(s):  
P. G. McKenna ◽  
A. A. Yasseen
Keyword(s):  
Thymidine Kinase ◽  
Wild Type ◽  
Ethyl Methane Sulphonate ◽  
Leukaemia Cell ◽  
Leukaemia Cell Line ◽  
Ethyl Methane ◽  
Chemical Mutagens ◽  
Methane Sulphonate ◽  
Increased Sensitivity ◽  
Unit Dose

SUMMARYWild-type Friend murine leukaemia (clone 707) cells and two thymidinekinase-deficient subclones, 707BUE and 707BUF, were compared for sensitivity to killing and mutagenesis by the chemical mutagens, ethyl methane sulphonate (EMS),N-methyl-N′-nitro-N-nitrosoguanidine (MNNG), mitomycin C (MMC), and methyl methane sulphonate (MMS). The two thymidine-kinase-deficient subclones were significantly more sensitive to killing by each of the four chemical mutagens than were wild-type cells. The increased sensitivity to killing by the four mutagens was also reflected in increased mutagenesis (per unit dose of mutagen) to 6-thioguanine resistance. In the light of these results, the significance of thymidine kinase in DNA repair and mutagenesis is discussed.

Quantitative variation induced by gamma rays, ethyl methane sulphonate, and hydrazine hydrate in mung bean (Phaseolus aureus Roxb.)

1984 ◽  
Vol 26 (4) ◽  
pp. 492-496 ◽  
Author(s):  
Irfan A. Khan
Keyword(s):  
Gamma Rays ◽  
Seed Weight ◽  
Genetic Advance ◽  
Ethyl Methane Sulphonate ◽  
Ethyl Methane ◽  
Plant Yield ◽  
Mean Values ◽  
Methane Sulphonate ◽  
The Mean ◽  
100 Seed Weight

Induced variability was studied in the quantitative characters of mung bean (Phaseolus aureus Roxb.) after treatment with gamma rays, ethyl methane sulphonate (EMS), and hydrazine hydrate (HZ) in M1, M2, and M3 generations. The characters evaluated were seeds per pod, 100-seed weight, and total plant yield. The variability increased in almost all of the characters in the M1 generation. The mean number of seeds per pod and 100-seed weight decreased, whereas the plant yield did not show any particular trend. There was an increase in the mean values after gamma irradiation and EMS treatments in the M2 generation. However, HZ fails to show the same response. However, the mean values increased in the M3 generation. Estimates of heritability were higher for 100-seed weight, followed by plant yield, and then seeds per pod in the M3 generation. Coefficient of genotypic variation and genetic advance were high for the plant yield in M2 and M3 generations. In general, the genotypic coefficient of variation values, heritability, and genetic advance increased more in M3 as compared with M2, indicating that the significant gain could possibly be achieved through selection in M3 generation.Key words: quantitative, heritability, mutagenesis.

scholarly journals Morphology and Yield Parameters and Biochemical Analysis of Soybean (Glycine max (L.) Mrr.) Using Gamma Rays, EMS and DES Treatment

2015 ◽  
Vol 35 ◽  
pp. 50-58 ◽  
Author(s):  
P. Gopinath ◽  
P. Pavadai
Keyword(s):  
Glycine Max ◽  
Plant Height ◽  
Seed Yield ◽  
Gamma Rays ◽  
Fruit Size ◽  
Mean Value ◽  
Ethyl Methane Sulphonate ◽  
Ethyl Methane ◽  
Yield Parameters ◽  
Methane Sulphonate

Soybean (Glycine max (L.) Merr.) var. Co-1 seeds were treated with physical mutagen namely such as gamma rays and chemical mutagen namely such as Ethyl Methane Sulphonate (EMS) and Diethyl Sulphate (DES). Various concentrations of gamma rays 10, 20, 30, 40, 50 and 60 KR, EMS (0.1, 0.2, 0.3, 0.4, 0.5 and 0.6 %) and DES (0.1, 0.2, 0.3, 0.4, 0.5 and 0.6 %). The morphological parameters such as days to first flower, plant height, number of cluster per plant, number of pod per plan, seed yield per plant, number of branches per plant, number of leaves per plant, protein content and oil content increasing with increasing level of some doses gamma rays, Ethyl methane sulphonate and Diethyl sulphate treatment in M2 and M3 generation were carried out. A number of mutants were recorded in M2 and M3 generations for one or more traits viz., plant height, maturity, branching, pigmentation, fruit size and yield were isolated in M2 and M3 generation. Mean for various characters increased at mutagenic treatment than control. The yield parameters like plant height, number of cluster per plant, number of seeds per plant and seed yield per plant were recorded the moderated and high mean value in the 50 KR of gamma rays 0.5% of EMS and 0.4% of DES treated population with compared to control plants.

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