scholarly journals Antioxidant activities and phenolic constituents of Cephalotaxus oliveri Mast. aerial parts

2012 ◽  
Vol 77 (4) ◽  
pp. 437-451 ◽  
Author(s):  
Lingbin Zeng ◽  
L.L.P. Vrijmoed
Keyword(s):  
Phenolic Compounds ◽  
Methanol Extract ◽  
Phenolic Content ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Antioxidant Potential ◽  
Total Phenolic ◽  
Scavenging Activity ◽  
Aerial Parts

The present study was to analyze the antioxidant activity and the responsible chemical constituents in Cephalotaxus oliveri Mast. aerial parts. DPPH (2,2-diphenyl-1-picryl hydrazyl), ABTS [2,2?-azinobis(3-ethylbenzothiazoline- 6-sulfonic acid)], reducing power and total phenolic content assays indicate that the methanol extract of the aerial parts is the most potent radical-scavenger and reducing agent with the highest level of phenolic content among the test extracts. The highly positive linear correlations implicate that the four assays have similar capacity to predict the antioxidant potential of the aerial parts and phenolic compounds present contribute significantly to DPPH and ABTS radical scavenging activity and reducing power of the extracts. The plant also displayed considerable superoxide anion radical scavenging activity. LC-MS/MS and GC-MS analyses resulted in the identification of 22 compounds in the methanol extract, among which are 15 phenolic compounds. The total amount of the phenolic compounds in the methanol extract determined by HPLC method was more than 5622.39 ?g/g dry weight. The considerable antioxidant potential and a high content of phenolic antioxidants suggest that C. oliveri aerial parts are a potential source of natural antioxidants.

scholarly journals Effects of solvent extraction system on antioxidant activity of Lamium purpureum L.

2017 ◽  
Vol 71 (5) ◽  
pp. 361-370
Author(s):  
Slavica Grujic ◽  
Ana Dzamic ◽  
Violeta Mitic ◽  
Vesna Stankov-Jovanovic ◽  
P.D. Marin ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Methanol Extract ◽  
Phenolic Content ◽  
Radical Scavenging Activity ◽  
Antioxidant Properties ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Total Phenolic ◽  
Radical Scavenger ◽  
Scavenging Activity

Antioxidant and free radical scavenging activity of methanol, ethanol, ethyl acetate and chloroform extracts of aerial parts of Lamium purpureum L. was determined by DPPH, ABTS, FRAP and TRP assays. Contents of flavonoids and phenols were also investigated. The total phenolic content in the extracts, determined using Folin?Ciocalteu assay, ranged between 8.57 to 128.00 mg GAE/g d.e. while concentrations of flavonoids in the extracts varied from 24.20 to 39.80 mg QuE/g d.e. The highest phenolic content was found in methanol extract (128.00 mg GAE/g d.e.). The highest content of total flavonoids was identified in the methanol extract (39.80 mg QuE/g d.e.) and the lowest was in the chloroform (24.30 mg QuE/g d.e.). DPPH scavenging of the extracts was determined and obtained IC50 values ranged from 0.12 to 3.12 mg/mL of solution. The values of ABTS radical scavenging activity ranged from 0.35 to 1.80 mg AA/g. The highest ABTS antiradical activity was registered for methanol extract. The FRAP value was found within the range 0.08 to 1.04 ?mol Fe/mg. The best radical scavenger was methanol (1.04 ?mol Fe/mg). In reducing power assay different extracts of L. purpureum showed increasing of activity with increased concentration, and all extracts possessed substantial dose dependent antioxidant activity. The best reducing capacity was obtained with methanol extract of L. purpureum (0.0132 mg AA/mL). The results in this study confirmed that L. purpureum possesses moderate antioxidant properties.

scholarly journals Effect of Artificial Shading and Temperature on Radical Scavenging Activity and Polyphenolic Composition in Sweetpotato (Ipomoea batatas L.) Leaves

2003 ◽  
Vol 128 (2) ◽  
pp. 182-187 ◽  
Author(s):  
Md. Shahidul Islam ◽  
Makoto Yoshimoto ◽  
Koji Ishiguro ◽  
Shigenori Okuno ◽  
Osamu Yamakawa
Keyword(s):  
Phenolic Compounds ◽  
Ipomoea Batatas ◽  
Phenolic Content ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Cultivar Differences ◽  
Total Phenolic ◽  
Scavenging Activity ◽  
Caffeoylquinic Acid ◽  
Phenolic Components

The phenolic content and the radical scavenging activity were compared in leaves of sweetpotato (Ipomoea batatas L.) cultivars Shimon-1, Kyushu-119 and Elegant Summer grown under different temperature and shading conditions. Compared to cultivar differences, there was less effect of temperature and shading on the total phenolic content in sweetpotato leaves, however certain polyphenolic components differed widely among the treatments. The positive correlation between the radical scavenging activity and the level of total phenolics (r = 0.62) suggests that phenolic compounds are important antioxidant components of sweetpotato leaves. All the reverse-phase high-performance liquid chromatography (RP-HPLC) profiles of the cultivars tested showed peaks at the same retention times but peak areas of individual phenolic compounds differed with respective temperature and shading treatments. The phenolic compounds identified in the sweetpotato leaf were caffeic acid, chlorogenic acid, 4,5-di-O-caffeoylquinic acid, 3,5-di-O-caffeoylquinic acid, 3,4-di-O-caffeoylquinic acid, and 3,4,5-tri-O-caffeoylquinic acid. Most of the phenolic compounds were highest in leaves from plants grown at 20 °C without shading except 4,5-di-O-caffeoylquinic acid. The results indicate that growing leaves under moderately high temperatures and in full sun enhances the accumulation of phenolic components. These phenolic components have possible value in enhancing human health.

scholarly journals Antioxidant, Anti-Inflammatory and Antibacterial Activities of the Seeds of A Sri Lankan Variety of Carica Papaya

2019 ◽  
Vol 12 (2) ◽  
pp. 539-547
Author(s):  
Akshani Anjula Wijesooriya ◽  
Srianthie A. Deraniyagala ◽  
Chamari M. Hettiarachchi
Keyword(s):  
Ascorbic Acid ◽  
Phenolic Content ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Total Phenolic ◽  
Sri Lankan ◽  
Scavenging Activity ◽  
Flavonoid Content ◽  
Anti Inflammatory

Plant based remedies are of much importance in healthcare due to low side effects. The effective constituents contained in seeds of a papaya have not been utilized efficiently in the production of medicines. The purpose of this study was driven towards determining the total phenolic content, total flavonoid content, antioxidant capacity, anti-inflammatory activity and antibacterial properties of the aqueous seeds extract of a Sri Lankan variety (Red Lady) of papaya (AESP). The AESP prepared according to the method of “Kasaya” in Ayurvedic medicine was used for the investigations according to standard procedures. Total phenolic content and the total flavonoid content of the AESP were 13.5±2.2 mg (pyrogallol equivalence)/g and 315.9±104.6 mg (quercetin equivalence)/g respectively. The 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activity of AESP ranged from 12.4% to 81.2% for concentrations of 1.0-20.0 mg/ml; ascorbic acid gave 31.7% - 91.9% activity. The AESP displayed strong reducing power compared to ascorbic acid in the reducing power assay. The hydroxyl radical scavenging activity of the AESP ranged from 6.6% to 69.1% for concentrations ranging from 20.0-95.0 mg/ml; ascorbic acid activity was 37.7%-74.9%. The nitric oxide radical scavenging activity of AESP was 23.9%-62.7% for concentrations of 2.0-20.0 µg/ml; ascorbic acid gave 26.8%-63.5% activity. AESP concentrations ranging from 75.0-150.0 µg/ml exhibited human red blood cell (HRBC) membrane stabilization protection of 15.5%-22.7% compared to 50.8%-58.4% for aspirin. The AESP showed antibacterial activity against Bacillus subtilis and Staphylococcus aureus bacterial species. AESP possess promising antioxidant, anti-inflammatory and antibacterial activity. The aqueous decoction of the discarded seeds of papaya would facilitate remedies for many diseases in which radicals are implicated as well as assist against certain bacterial infections and also has an anti-inflammatory potential.

scholarly journals Hypericum perforatum Mikrokapsüllerinin Ayran (İçilebilir Yoğurt) Üretiminde Kullanımı

2021 ◽  
Vol 9 (11) ◽  
pp. 2013-2021
Author(s):  
Fadime Seyrekoğlu ◽  
Hasan Temiz
Keyword(s):  
Phenolic Compounds ◽  
Sensory Analysis ◽  
Phenolic Content ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Total Phenolic ◽  
Scavenging Activity ◽  
Dpph Radical ◽  
Dpph Radical Scavenging Activity ◽  
Dpph Radical Scavenging

H. perforatum, which is widely used in traditional medicine due to its bioactive compounds was extracted with ethanol-water (3:7). The extract was encapsulated with maltodextrin and gum arabic in a spray dryer in order to protect the phenolic compounds in its structure. Different amounts of microcapsules were added to our traditional drink, i.e. ayran (drinking yoghurt). The total phenolic content and DPPH radical scavenging activity of the microcapsules, extract of HP, and ayran samples were determined. The amount of total phenolic compounds in the microcapsule provided a superior effect than the extract. The ayran samples were supplemented with 2%, 3%, 4%, 5% and 6% of H. perforatum (HP) microcapsules and it is observed that total phenolic content (TPC) and DPPH radical scavenging activity indicated an increase with concentration. TPC and DPPH activity were determined as 256.94 mg GAE / 100mL and 78.05% for 6% of HP microcapsules supplemented samples. As a result of the sensory analysis, ayran samples which supplemented with 4% of HS microcapsule gained the highest scores by the panellists and received more appreciation than the control group. According to sensory analysis, HP4 (ayran produced with 4% HP added microcapsule) sample was determined as the best sample, while the HP6 (Ayran produced with 6% HP added microcapsule) sample had the highest scores in terms of DPPH scavenging activity and TPC results. The overall results of this study revealed that 4% HP supplemented ayran can be produced with its increased health benefits and desirable properties. In this study, the use of H. perforatum microcapsules in ayran, its effect on antioxidant and phenolic components, the usage rates and acceptability of microcapsules were investigated.

scholarly journals Feasibility of Using Bitterness and Foaming Properties as Surrogate Instrument in Assessing Antioxidant Potentials of Selected Eggplant Cultivars

2019 ◽  
Vol 4 (2) ◽  
Author(s):  
Eunice M Ogunbusola ◽  
Bode Daramola
Keyword(s):  
Phenolic Content ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Total Phenolic ◽  
Scavenging Activity ◽  
Health Promoting ◽  
Foaming Capacity ◽  
Antioxidant Markers ◽  
Insight Into

This study attempt to circumvent the hurdle associated with the utilization of laborious and expensive procedure to assess some health promoting properties of eggplant fruits. The feasibility of using simple and cheap procedure as a surrogate tool to gain insight into the antioxidant potentials of eggplant cultivars was evaluated. Some eggplants cultivars obtained fresh were used for the work. Extracts from the garden eggs were analysed for relative reducing power, total phenolic content and radical scavenging activity using DPPH. Physical and Sensory attributes such as foaming ability and stability, and level of bitterness were also carried out. Results showed that assessment of some antioxidant markers notably total phenolic content (2.266 – 6.406), radical scavenging activity (35.00 – 82.50) and relative reducing power (0.139 – 1.145), showed positive correlation (r = 0.60281 - 0.803348; r = 0.861877 -0.905333; and r = 0.851459 - 0.958941) with bitterness, foaming capacity and foaming stability respectively. Simple and cheap quality control protocol could be used as surrogate tool to gain insight into the antioxidant markers, a health promoting endowment of some eggplants. Keywords - Eggplants, bitterness, foaming capacity, surrogate tool, antioxidant potentials.

scholarly journals Effect of grinding and extraction conditions on the determination of antioxidant activity and phenolic acids in barley

2021 ◽  
Author(s):  
Bing Zhou ◽  
Zhao Jin ◽  
Paul B. Schwarz ◽  
Yin Li
Keyword(s):  
Phenolic Acid ◽  
Total Phenolic Content ◽  
Phenolic Content ◽  
Antioxidant Activities ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Total Phenolic ◽  
Scavenging Activity ◽  
Dpph Scavenging

AbstractIn the present study, the objective was to evaluate the effects of barley kernel size, grind level, and extract solvent on the antioxidant activities associated with total phenolic content and phenolic acid compositions. Three barley varieties (Kindred, Azure, and Tradition), were used and the results showed that with the exception of superoxide anion radical scavenging activity, thin kernel size fraction (2.0 mm) showed much higher antioxidant activities and total phenolic content than those in the 2.4 mm and 2.8 mm fractions when averaged across barley cultivar, grind level, and extract solvent. A similar trend was found for individual phenolic acid compositions among kernel sizes. Simple correlation analysis revealed that total phenolic content showed strong correlation with DPPH· scavenging activity, ABTS + scavenging activity, and reducing power. Generally, fine grind and 80% acetone extract showed averaged highest values in antioxidant activities, total phenolic content, and individual phenolic acid compositions. Stepwise linear regression showed that extract solvent was the most important factor for DPPH· scavenging activity, ABTS + scavenging activity, reducing power, and iron chelating activity. The sum of syringic acid and caffeic acid exhibited a dominant role in explaining the major variation in antioxidant activities except for superoxide anion radical scavenging activity.

scholarly journals Comparison of ABTS, DPPH, Phosphomolybdenum Assays for Estimating Antioxidant Activity and Phenolic Compounds in Five Different Plant Extracts

2018 ◽  
Vol 75 (2) ◽  
pp. 110
Author(s):  
Arabela UNTEA ◽  
Alexandra LUPU ◽  
Mihaela SARACILA ◽  
Tatiana PANAITE
Keyword(s):  
Antioxidant Activity ◽  
Free Radical ◽  
Phenolic Compounds ◽  
Phenolic Content ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Total Phenolic ◽  
Scavenging Activity ◽  
Total Phenols ◽  
Walnut Leaves

Many plants and their constituents (carotenoids, flavonoids and other phenolic compounds), action to inhibit the free radical by the use of antioxidant activity, helping to a better efficiency of nutrients utilization. The purpose of this study was to investigate the antioxidant activity and phenolic content of five different plants, known for their antioxidant potential. Total phenols were determined by FolinCiocalteumethod, with few modifications. The antioxidant capacity was evaluated by DPPH, ABTS radical-scavenging activity and phosphomolybdenum method. The results showed that the highest antioxidant activity was obtained for walnut leaves in terms of ABTS evaluation and for bilberry leaves by DPPH and phosphomolybdenum methods. In the present study, peppermint, walnut and bilberry leaves proved to have an important antioxidant activity and also a total phenolic content.

Extrusion and solid-state fermentation with Aspergillus oryzae on the phenolic compounds and radical scavenging activity of pecan nut (Carya illinoinensis) shell

2021 ◽  
Vol ahead-of-print (ahead-of-print) ◽  
Author(s):  
Juliana Villasante ◽  
Johanan Espinosa-Ramírez ◽  
Esther Pérez-Carrillo ◽  
Erick Heredia-Olea ◽  
MariaPilar Almajano
Keyword(s):  
Solid State ◽  
Phenolic Compounds ◽  
Aspergillus Oryzae ◽  
Solid State Fermentation ◽  
Phenolic Content ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Total Phenolic ◽  
Scavenging Activity ◽  
Content Type

PurposeSolid-state fermentation (SSF) has been highlighted as an alternative to obtain valuable compounds using agro-industrial wastes as a substrate. The present study evaluated the impact of extrusion combined with SSF on the production of phenolic compounds and their antioxidant activity using pecan nut shell (PWS) as a substrate.Design/methodology/approachPWS and extruded pecan nut shell (PWSE) were fermented for 120 h at 30°C using Aspergillus oryzae (A. oryzae). Samples were withdrawn from incubator at 6 h, 12 h and then every 12 h until 120 h fermentation. PWS and PWSE samples were extracted. The total phenolic content (TPC) and radical scavenging activity (RSA) extracts were characterized from the resulting extracts.FindingsThe use of PWSE yielded higher A. oryzae biomass and at a higher rate after 120 h of fermentation (PWS 75.74% vs PWSE 87.50%). In general, the TPC and the RSA increased with fermentation time. However, the PWSE yielded significantly higher (p < 0.05) TPC and RSA values after SSF in comparison to the nonextruded PWS. Condensed tannins showed different trends depending on the fermented substrate. Overall, results showed that the extrusion pretreatment joint with the SSF represents a good alternative to raise the phenolic content and antiradical activity of lignocellulosic materials such as PWSs.Originality/valueThis study offers valuable information that may be used by the pecan walnut industry to valorize the shell coproduct as a substrate to produce functional ingredients or fungal enzymes.

scholarly journals Antioxidant Property of Aerial Parts and Root ofPhyllanthus fraternusWebster, an Important Medicinal Plant

2014 ◽  
Vol 2014 ◽  
pp. 1-7 ◽  
Author(s):  
Richa Upadhyay ◽  
Jitendra Kumar Chaurasia ◽  
Kavindra Nath Tiwari ◽  
Karuna Singh
Keyword(s):  
Free Radical ◽  
Aerial Part ◽  
Radical Scavenging Activity ◽  
Spectroscopic Method ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Free Radical Scavenging ◽  
Total Phenolic ◽  
Scavenging Activity ◽  
Aerial Parts

In present study free radical scavenging potential of aerial parts and root ofPhyllanthus fraternuswas investigated. Extraction was done in water and ethanol. Total antioxidant capacity was measured by DPPH free radical scavenging method; ethanolic extract of aerial part was most potent in activity with 50% inhibition at 258 μg/mL concentration. Lipid peroxidation (LPO) was measured in terms of thiobarbituric acid-reactive substances (TBARS) by using egg-yolk homogenates as lipid-rich media with EC50of aerial part (ethanolic) 1522 μg/mL which was found to be most active. Superoxide (SO) radical scavenging activity was measured using riboflavin-light-nitroblue tetrazolium assay. Ethanolic and aqueous extract of both aerial part and root was almost similar in superoxide radical scavenging activity. Reducing power was determined on the basis ofFe3+-Fe2+transformation in the presence of extract. Total phenolic and flavonoid contents were also measured by spectroscopic method. Results showed that the ethanolic fraction of aerial part is most active towards antioxidant potential and this activity is related to its polyphenolic content and reducing potential. Thus,P. fraternusextract can be used as potent natural antioxidant.

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