scholarly journals Immunohistological characteristics of collagen, laminin and sialoadhesin in the rat submandibular salivary gland during ontogenesis

2003 ◽  
Vol 50 (4) ◽  
pp. 179-183
Author(s):  
Ivan Dozic ◽  
Miodrag Colic
Keyword(s):  
Extracellular Matrix ◽  
Monoclonal Antibodies ◽  
Salivary Gland ◽  
Postnatal Development ◽  
Basal Membrane ◽  
Myoepithelial Cells ◽  
Matrix Proteins ◽  
Submandibular Salivary Gland ◽  
Ductal Cells ◽  
Positive Reactivity

The aim of this study was to investigate the expression of collagen, laminin and sialoadhesin in the rat submandibular salivary gland during postnatal development (1st, 30th,and 60th day) by using various monoclonal antibodies (mAbs) RMC-23 (specific for collagen),a6?1 (specific for laminin) and ED3 (specific for sialoadhezin). These components of extracellular matrix were detected. RMC-23 mAb showed strong positivity to the basal membranes of the ductal system (intercalated, striated and excretory ducts) and of intersticium. Increased expression in the basal membrane of acini during development of glands was noted. Similar immunoreactivity was shown by?mAb but the intersticium showed a negative reaction to 1a6 this antibody. Positive reactivity of ?1a6 mAb of epithelial ductal cells, particularly of the neonatal animals, was found. In contrast to ? 1a6 and RMC23 mAbs, ED3 mAb was increasingly expressed in the myoepithelial cells during ontogenesis. Our findings regarding the immunoreactivity of collagens and laminins are in accordance with the findings of other autors. The very interesting finding of sialoadhesin in myoepithelial cells of the rat submandibular salivary gland, which is not described in literature and needs further investigation. Our results suggest that adhesion molecules and extracellular matrix proteins have an important biochemical role during postnatal development of the submandibular salivary gland.

Cytokeratin polypeptide expression during the histogenesis of guinea pig submandibular salivary gland

Development ◽  
1987 ◽  
Vol 100 (4) ◽  
pp. 699-711
Author(s):  
G. Marshak ◽  
O. Leitner ◽  
B. Geiger
Keyword(s):  
Monoclonal Antibodies ◽  
Salivary Gland ◽  
Guinea Pig ◽  
Salivary Glands ◽  
Broad Spectrum ◽  
Submandibular Salivary Gland ◽  
Basal Cells ◽  
Ductal Cells ◽  
Terminal Buds

The present study was directed towards the characterization of cell-specific histogenetic markers for the various epithelial elements of the adult and the developing guinea pig submandibular salivary gland. We have employed immunofluorescent labelling using three cytokeratin monoclonal antibodies, for which the polypeptide specificities towards guinea pig cytokeratins were determined. All the epithelial elements of the adult gland were positively labelled with two monoclonal antibodies, namely KG 8.13 (‘broad spectrum’ anti-cytokeratin) and antibody Ks B.18 (reactive with a simple cytokeratin-specific polypeptide of 49 X 10(3) Mr). Antibody KS 8.58 (reactive with a guinea pig cytokeratin polypeptide of 50 X 10(3) Mr) labelled the basal cells of the large ducts, as well as the myoepithelium. During development of the gland, the submandibular anlage and its primary and secondary branches with their terminal buds, were uniformly labelled with the three antibodies; however, the cytokeratin polypeptides reactive with antibody KS 8.58, which were apparently expressed in all cells of the developing ducts, gradually disappear from most of the ductal cells, starting at about 6 weeks of gestation, and remain only in the basal or reserve cells of the large ducts and the myoepithelium. These observations support the notion that the basal cells retain at least some of the properties of the embryonic glandular epithelium and could be considered as pluripotent reserve cells which may function as progenitors for other epithelial elements in the salivary glands epithelia.

Mediation of endothelial injury following neutrophil adherence to extracellular matrix

1993 ◽  
Vol 264 (4) ◽  
pp. L401-L405 ◽  
Author(s):  
R. A. Kaslovsky ◽  
L. Lai ◽  
K. Parker ◽  
A. B. Malik
Keyword(s):  
Extracellular Matrix ◽  
Endothelial Cells ◽  
Monoclonal Antibodies ◽  
Endothelial Permeability ◽  
Endothelial Injury ◽  
Matrix Proteins ◽  
Extracellular Matrix Components ◽  
The Matrix ◽  
Neutrophil Adherence ◽  
Permeability Increase

Since polymorphonuclear leukocytes (PMN) rapidly migrate across the endothelial barrier and attach to extracellular matrix components, we tested the hypothesis that adhesion of PMN to matrix proteins can mediate endothelial injury following PMN activation. Studies were made using gelatin- and fibronectin-coated polycarbonate microporous filters (10 microns thick) on which confluent monolayers of bovine pulmonary microvessel endothelial cells were grown. PMN were layered either directly onto endothelial cells (at a ratio of 10:1) (“upright system”) or onto gelatin- and fibronectin-coated filters with the endothelial monolayer grown on the underside of the filter without contact between PMN and endothelial cells (“inverted system”). PMN were activated with phorbol 12-myristate 13-acetate (PMA; 5 x 10(-9) M) in both systems. PMN activation increased endothelial permeability to 125I-labeled albumin in upright as well as inverted systems. Pretreatment of PMN with anti-CD18 monoclonal antibodies IB4 or R15.7, which inhibited PMN adherence to matrix constituents as well as to endothelial cells, prevented the permeability increase in both configurations. This effect of anti-CD18 monoclonal antibodies (mAbs) was not ascribed to a reduction in PMN activation, since PMA-induced superoxide generation was unaffected. We conclude that activation of PMN adherent to extracellular matrix proteins increases endothelial permeability to albumin and that this response is dependent on PMN adhesion to the matrix. The results support the concept that PMN-mediated increase in endothelial permeability is the result of “targeted” release of PMN products independent of whether the PMN are adherent to the extracellular matrix or the endothelium.

scholarly journals Phenotypic Changes of Calponin in Salivary Gland Myoepithelial Cells during Postnatal Development of Rats.

2000 ◽  
Vol 33 (2) ◽  
pp. 73-79 ◽  
Author(s):  
Miyako Namba ◽  
Kazuto Yamada ◽  
Wataru Kudeken ◽  
Gho Mizutani ◽  
Kouji Tsukitani ◽  
...  
Keyword(s):  
Salivary Gland ◽  
Postnatal Development ◽  
Myoepithelial Cells ◽  
Phenotypic Changes

scholarly journals Morphologic Characteristics of the Submandibular Salivary Gland of the Collared Peccary (Tayassu Tajacu)

2018 ◽  
Vol 35 (02) ◽  
pp. 116-121
Author(s):  
Gabriela de Souza Reginato ◽  
Cristina de Sousa Bolina ◽  
Moacir Franco Oliveira ◽  
Sonia Regina Yokomizo Almeida ◽  
Ii-sei Watanabe ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Salivary Gland ◽  
Salivary Glands ◽  
Secretory Granules ◽  
Morphological Characteristics ◽  
Acinar Cells ◽  
Collagen Fibers ◽  
Submandibular Salivary Gland ◽  
Ductal Cells ◽  
Collared Peccary

Introduction Most salivary glands is located on the inside and around the oral cavity, and are divided into major and minor salivary glands. The aim of the present study was to describe the structural and ultrastructural morphological characteristics of the lingual tissue of the submandibular glands of the collared peccary (Tayassu tajacu). Materials and Methods The submandibular glands (n = 10) of adult male collared peccaries ( T. tajacu) were used for histological and ultrastructural analysis. The techniques used were light microscopy, scanning electron microscopy (SEM) and transmission electron microscopy (TEM). Results The submandibular salivary glands of the collared peccary (T. tajacu) showed a capsule formed by a connective tissue containing the acinus and duct cells. Histologically, the nuclei located at the basal region of the cells was observed. The light polarized microscopy clearly showed the presence of type I and type III collagen. In the SEM image, the submandibular salivary gland revealed a round aspect separated in several lobules with bundles of collagen fibers. The vibratome sections showed the groupings of acinar cells, with intermingled secretory ducts containing vessels of different diameters. The secretory granules were noted in the apical portion of the acinar and ductal cells. The thick bundles of collagen fibers formed a glandular capsule and were identified around of the acinar and ductal cells in three-dimensional SEM images. The TEM images showed a number of secretory granules, especially in the apical region of the cytoplasm of the acinar cells and in the basal portion of the nuclei. The granular endoplasmic reticulum area, the euchromatic nuclei and the cytoplasmic projections may be seen. Mucous acinar cells separated by fine collagen fibers were also observed. Conclusion The morphological characteristics of the submandibular gland of the collared peccary is similar to that of other mammals with the same eating habits and habitat.

scholarly journals Localization of Vesicular Inhibitory Amino Acid Transporter (VIAAT) in the Submandibular Salivary Gland Throughout the Postnatal Development of Mice

2015 ◽  
Vol 33 (1) ◽  
pp. 113-118 ◽  
Author(s):  
Yanyong Toomsan ◽  
Surang Chomphoo ◽  
Sawetree Pakkarato ◽  
Masahiko Watanabe ◽  
Hisatake Kondo ◽  
...  
Keyword(s):  
Salivary Gland ◽  
Amino Acid ◽  
Postnatal Development ◽  
Amino Acid Transporter ◽  
Submandibular Salivary Gland ◽  
Acid Transporter

Immunoexpression of extracellular matrix proteins in human salivary gland development

2004 ◽  
Vol 112 (6) ◽  
pp. 548-551 ◽  
Author(s):  
Cristiane Furuse ◽  
Patricia Ramos Cury ◽  
Ney Soares de Araujo ◽  
Vera Cavalcanti de Araujo
Keyword(s):  
Extracellular Matrix ◽  
Salivary Gland ◽  
Extracellular Matrix Proteins ◽  
Matrix Proteins ◽  
Human Salivary Gland ◽  
Gland Development

Epithelial-Myoepithelial Carcinoma of Salivary Glands

1992 ◽  
Vol 101 (6) ◽  
pp. 540-542 ◽  
Author(s):  
John G. Batsakis ◽  
Adel K. El-Naggar ◽  
Mario A. Luna
Keyword(s):  
Salivary Gland ◽  
Salivary Glands ◽  
Cell Population ◽  
Phenotypic Expression ◽  
Myoepithelial Cells ◽  
Salivary Gland Neoplasms ◽  
Myoepithelial Carcinoma ◽  
Parotid Glands ◽  
Ductal Cells ◽  
Epithelial Myoepithelial Carcinoma

Epithelial-myoepithelial carcinomas comprise approximately 1 % of all salivary gland neoplasms. They are preponderantly tumors of the parotid glands with a relatively low mortality but a decided locoregional aggressiveness. Histopathologically, the carcinomas are characterized by a dual cell population of epithelial (ductal) cells and myoepithelial cells. These cells vary in their dominance and phenotypic expression

scholarly journals УЛЬТРАСТРУКТУРНА ОРГАНІЗАЦІЯ ПІДНИЖНЬОЩЕЛЕПНОЇ СЛИННОЇ ЗАЛОЗИ ЩУРА В НОРМІ

2020 ◽  
pp. 23-26
Author(s):  
Mykhalevych Marta
Keyword(s):  
Endothelial Cells ◽  
Salivary Gland ◽  
Normal State ◽  
Cytoplasmic Membrane ◽  
Acinar Cells ◽  
Basal Membrane ◽  
Submandibular Salivary Gland ◽  
Basal Surface ◽  
Membrane Pores ◽  
Peripheral Area

This publication demonstrates ultrastructures chracteristics of submandibular salivary gland of rats.A result of the ultrastructural research of the submandibular salivary gland in the normal state demonstrates serocytes of acinar cells are located compactly, densely adjacent to each other. On the basal surface of serocytes, plasmolemma contains shallow invaginations. Nuclei of serocytes are somewhat displaced into the basal part of the cell. In the peripheral area of the endothelial cells, in their cytoplasmic membrane, pores and fenesters occur. The basal membrane of the capillaries is two-layered, clearly contoured, sometimes wavy, consisting of light and dense plates.

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