Receptor activator of nuclear factor kappa B (RANK) as a determinant of peri-implantitis

Background/Aim. Peri-implantitis presents inflammatory process that affects soft and hard supporting tissues of osseointegrated implant based on inflammatory osteoclastogenesis. The aim of this study was to investigate whether receptor activator of nuclear factor kappa B (RANK) concentrations in peri-implant crevicular fluid could be associated with clinical parameters that reflect inflammatory nature of peri-implantitis. Methods. The study included 67 patients, 22 with diagnosed peri-implantitis, 22 persons with healthy peri-implant tissues and 23 patients with periodontitis. Clinical parameters from each patient were recorded and samples of peri-implant/gingival crevicular fluid were collected for the enzyme-linked immunosorbent assay (ELISA) analysis. Results. RANK concentration was significantly increased in samples from the patients with periimplantitis when compared to healthy implants (p < 0.0001), where the average levels were 9 times higher. At the same time RANK concentration was significantly higher in periimplantitis than in periodontitis sites (p < 0.0001). In implant patients pocket depths and bleeding on probing values were positively associated with high RANK concentrations (p < 0.0001). Conclusion. These results revealed association of increased RANK concentration in samples of periimplant/ gingival crevicular fluid with peri-implant inflammation and suggests that RANK could be a pathologic determinant of peri-implantitis, thereby a potential parameter in assessment of peri-implant tissue inflammation and a potential target in designing treatment strategies.

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Identification of the osteoprotegerin/receptor activator of nuclear factor-kappa B ligand system in gingival crevicular fluid and tissue of patients with chronic periodontitis

Journal of Periodontal Research ◽

10.1111/j.1600-0765.2006.00883.x ◽

2006 ◽

Vol 41 (4) ◽

pp. 354-360 ◽

Cited By ~ 83

Author(s):

H.-K. Lu ◽

Y.-L. Chen ◽

H.-C. Chang ◽

C.-L. Li ◽

M. Y.-P. Kuo

Keyword(s):

Chronic Periodontitis ◽

Nuclear Factor Kappa B ◽

Gingival Crevicular Fluid ◽

Nuclear Factor ◽

Nuclear Factor Kappa ◽

Ligand System ◽

Receptor Activator ◽

Crevicular Fluid

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Gingival Crevicular Fluid, Serum Levels of Receptor Activator of Nuclear Factor-Kappa B Ligand, Osteoprotegerin, Interleukin-17 in Rheumatoid Arthritis and Osteoporosis Patients With Periodontal Disease

Journal of Periodontology ◽

10.1902/jop.2013.120595 ◽

2013 ◽

pp. 1-13 ◽

Cited By ~ 7

Author(s):

Pınar Gümüş ◽

Eralp Buduneli ◽

Başak Bıyıkoğlu ◽

Kenan Aksu ◽

Fulden Saraç ◽

...

Keyword(s):

Rheumatoid Arthritis ◽

Periodontal Disease ◽

Nuclear Factor Kappa B ◽

Gingival Crevicular Fluid ◽

Serum Levels ◽

Interleukin 17 ◽

Nuclear Factor ◽

Nuclear Factor Kappa ◽

Receptor Activator ◽

Crevicular Fluid

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The Relationship between Serum and Gene Expression Levels of RANK, RANKL and Osteoprotegerin Inflammatory Pathway with Unstable Angina: A Case-control Study

Iranian Journal of Allergy Asthma and Immunology ◽

10.18502/ijaai.v20i4.6957 ◽

2021 ◽

Author(s):

Alireza Farrokhian ◽

Mahtab Miraftab ◽

Minoo Chenari ◽

Hossein Akbari ◽

Hassan Nikoueinejad ◽

...

Keyword(s):

Gene Expression ◽

Unstable Angina ◽

Nuclear Factor Kappa B ◽

Mononuclear Cells ◽

Serum Levels ◽

Enzyme Linked Immunosorbent Assay ◽

Nuclear Factor ◽

Inflammatory Pathway ◽

Nuclear Factor Kappa ◽

Receptor Activator

Osteoprotegerin (OPG), receptor activator of nuclear factor-kappa B (RANK) and receptor activator of nuclear factor-kappa B ligand (RANKL), the members of the tumor necrosis factor (TNF) family, have multiple effects on bone metabolism, endocrine functions and, as an inflammatory pathway, in the immune system. This study tried to determine the association of the OPG/RANKL/RANK axis with the severity of unstable angina (UA) as an inflammatory condition. Our study involved 50 patients with UA and 50 healthy people. Serum and peripheral blood mononuclear cells were isolated from all participants. Serum levels and gene expression of OPG, RANKL, and RANK in mononuclear cells were measured by enzyme-linked immunosorbent assay (ELISA) and real-time polymerase chain reaction (RT-PCR), respectively. For each patient with UA, the thrombolysis in myocardial infarction (TIMI) and the global registry of acute coronary events (GRACE) scores were determined to evaluate the severity of the disease. Then we analyzed the relation of OPG, RANKL, and RANK levels with TIMI and GRACE scores in patients with UA. Discriminate analysis was used to predict the combinational models of such factors on the prediction of UA. Serum levels of OPG and RANKL (p<0.001) and gene expression of RANKL (p<0.001) were significantly more in patients than those in healthy ones. No relation was seen between the OPG/RANKL/RANK axis and the severity of UA according to TIMI and GRACE scores. Our study shows that serum level, as well as gene expression of OPG/RANKL/RANK axis neither, predicts the occurrence of UA nor shows any relationship with its severity.

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