Pinus muricata: Farjon, A.

Author(s):  
Keyword(s):  
2001 ◽  
Vol 79 (10) ◽  
pp. 1211-1216 ◽  
Author(s):  
Nina Wurzburger ◽  
Martin I Bidartondo ◽  
Caroline S Bledsoe

We used morphotyping and molecular methods to characterize ectomycorrhizas of bishop pine (Pinus muricata D. Don) and Bolander pine (Pinus contorta ssp. bolanderi (Parl.) Critchf.) from mixed conifer and hydric pygmy forests on the northern California coast. Sixteen ectomycorrhizal morphotypes were described, producing 15 internal transcribed spacer restriction fragment length polymorphism (ITS-RFLP) types, and 12 were identified via ITS sequencing. From a given site, all root tips of a specific morphotype produced identical ITS-RFLP patterns. However, sometimes two morphotypes produced the same ITS-RFLP type, and sometimes samples of the same morphotype from two different sites produced two different ITS-RFLP types. These results indicate that surveys of ectomycorrhizal fungi based on morphology alone are not sufficient, and that grouping morphotypes prior to molecular analysis can expedite the process. Ectomycorrhizas from mixed conifer included Russuloid sp., Tomentella sublilacina (Ellis & Holw.) Wakef., Tuber sp., and two Thelephoroid species. Ectomycorrhizas from hydric pygmy included two Dermocybe spp., a Cortinarius sp., two Thelephoroid spp., and Suillus tomentosus (Kauffman) Singer. Both plant communities contained Cenococcum geophilum Fr.:Fr. The hydric pygmy sites were more similar to each other than to the mixed conifer site (Jaccard similarity). The presence of ectomycorrhizal taxa in one plant community type may reflect biotic (host specificity) or abiotic (soil fertility or hydrology) adaptation.Key words: ectomycorrhiza, bishop pine, Pinus muricata, Bolander pine, Pinus contorta ssp. bolanderi, morphotyping, ITS-RFLP.


Author(s):  
Henry John Elwes ◽  
Augustine Henry
Keyword(s):  

Evolution ◽  
1983 ◽  
Vol 37 (2) ◽  
pp. 311 ◽  
Author(s):  
Constance I. Millar
Keyword(s):  

Genetics ◽  
1993 ◽  
Vol 135 (4) ◽  
pp. 1187-1196
Author(s):  
Y P Hong ◽  
V D Hipkins ◽  
S H Strauss

Abstract The amount, distribution and mutational nature of chloroplast DNA polymorphisms were studied via analysis of restriction fragment length polymorphisms in three closely related species of conifers, the California closed-cone pines-knobcone pine: Pinus attenuata Lemm.; bishop pine: Pinus muricata D. Don; and Monterey pine: Pinus radiata D. Don. Genomic DNA from 384 trees representing 19 populations were digested with 9-20 restriction enzymes and probed with cloned cpDNA fragments from Douglas-fir [Pseudotsuga menziesii (Mirb.) Franco] that comprise 82% chloroplast genome. Up to 313 restriction sites were surveyed, and 25 of these were observed to be polymorphic among or within species. Differences among species accounted for the majority of genetic (haplotypic) diversity observed [Gst = 84(+/- 13)%]; nucleotide diversity among species was estimated to be 0.3(+/- 0.1)%. Knobcone pine and Monterey pine displayed almost no genetic variation within or among populations. Bishop pine also showed little variability within populations, but did display strong population differences [Gst = 87(+/- 8)%] that were a result of three distinct geographic groups. Mean nucleotide diversity within populations was 0.003(+/- 0.002)%; intrapopulation polymorphisms were found in only five populations. This pattern of genetic variation contrasts strongly with findings from study of nuclear genes (allozymes) in the group, where most genetic diversity resides within populations rather than among populations or species. Regions of the genome subject to frequent length mutations were identified; estimates of subdivision based on length variant frequencies in one region differed strikingly from those based on site mutations or allozymes. Two trees were identified with a major chloroplast DNA inversion that closely resembled one documented between Pinus and Pseudotsuga.


1997 ◽  
Vol 99 (3) ◽  
pp. 291-294 ◽  
Author(s):  
F. Montenegro ◽  
V. Meneses ◽  
J.A. Wright
Keyword(s):  

1996 ◽  
Vol 74 (10) ◽  
pp. 1572-1583 ◽  
Author(s):  
M. Gardes ◽  
T. D. Bruns

We examined the species diversity of an ectomycorrhizal community in natural stands of bishop pine (Pinus muricata D. Don) to determine the correspondence between above- and below-ground views of species composition, spatial frequency, and abundance. We addressed this question by simultaneously sampling fruit bodies and ectomycorrhizae over a 4-year period. By using molecular methods based on polymerase chain reaction, we were able to identify the fungal symbionts directly from mycorrhizae in nearly all of the mycorrhizal morphotypes we found. Most species were either rare or low in abundance. Among the common species we observed three patterns: (i) some species, such as Russula xerampelina s.l. and Amanita francheti were well represented both above- and below-ground; (ii) some common fruiting species such as Suillus pungens were rare components belowground; (iii) some species that were common as mycorrhizae were represented poorly or unrepresented in the aboveground fruiting record. The latter was the case for Russula amoenolens, thelephoroid types (i.e., Tomentella sublilacina and thelephoroid-2), and a boletoid type. These results show that (i) the pattern of resource allocation to production of fruit bodies versus ectomycorrhizae varied among species, and (ii) the correspondence between above- and below-ground is imprecise at best at the community level. Keywords: mycorrhizal types, basidiocarps, sporocarps, fungal community, ecology of fungi, PCR, molecular ecology.


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