Effects of Dose Fractionation and Cycloheximide on the Heat-Shock Induction of Radiation Resistance in Primordial Germ Cells of the Fish Oryzias latipes

1985 ◽  
Vol 104 (1) ◽  
pp. 78 ◽  
Author(s):  
Y. Shimada ◽  
A. Shima ◽  
N. Egami
Keyword(s):  
Heat Shock ◽  
Germ Cells ◽  
Radiation Resistance ◽  
Primordial Germ Cells ◽  
Oryzias Latipes ◽  
Dose Fractionation ◽  
Heat Shock Induction

Sex differentiation of germ cells in the teleost, Oryzias latipes, during normal embryonic development

Development ◽  
1972 ◽  
Vol 28 (2) ◽  
pp. 385-395
Author(s):  
Noriyuki Satoh ◽  
Nobuo Egami
Keyword(s):  
Germ Cells ◽  
Meiotic Prophase ◽  
Sex Differentiation ◽  
Primordial Germ Cells ◽  
Oryzias Latipes ◽  
The Other ◽  
Histological Structure ◽  
The Mean ◽  
Further Development ◽  
Normal Embryonic Development

Mitotic and meiotic activities of germ cells during early development in the medaka, Oryzias latipes, are dealt with in this report. Primordial germ cells were obviously distinguishable from somatic cells 3 days after fertilization and began to proliferate about 7 days after fertilization. The mean number of primordial germ cells increased during a period of 7–10 days after fertilization, reaching about 90 immediately before hatching. Newly hatched fry could be classified into two types according to the number and the nucleic activity of germ cells in the gonadal rudiment. One type consisted of fry containing about 100 germ cells and no cells in the meiotic prophase. In the other type of fry the number of germ cells increased by mitotic divisions and some of the cells began to enter into the meiotic prophase. During the course of further development the fry of the former type differentiated into males and the latter into females. Therefore it can be concluded that the morphological sex differentiation of germ cells occurs at the time of hatching. However, no sexual differences in the histological structure of somatic elements in the gonad are observable at that time.

The vasa-like gene, olvas, identifies the migration path of primordial germ cells during embryonic body formation stage in the medaka, Oryzias latipes

2000 ◽  
Vol 42 (4) ◽  
pp. 317-326 ◽  
Author(s):  
Ai Shinomiya ◽  
Minoru Tanaka ◽  
Tohru Kobayashi ◽  
Yoshitaka Nagahama ◽  
Satoshi Hamaguchi
Keyword(s):  
Germ Cells ◽  
Primordial Germ Cells ◽  
Oryzias Latipes ◽  
Migration Path ◽  
Body Formation ◽  
Formation Stage

scholarly journals Immunocytochemical observation of the 90 KD heat shock protein (HSP90): high expression in primordial and pre-meiotic germ cells of male and female rat gonads.

1995 ◽  
Vol 43 (1) ◽  
pp. 67-76 ◽  
Author(s):  
S Ohsako ◽  
D Bunick ◽  
Y Hayashi
Keyword(s):  
Heat Shock ◽  
Heat Shock Protein ◽  
Germ Cells ◽  
Primordial Germ Cells ◽  
Gonad Development ◽  
Testicular Development ◽  
Female Rat ◽  
Male And Female ◽  
Primordial Follicles ◽  
Pachytene Spermatocytes

We used immunocytochemistry to detect the 90 KD major heat shock protein (HSP90), a potential regulator of gene expression, during male and female rat gonad development. In the Day 13.0 post-coital (dpc) fetal gonad, strong immunoreactivity to anti-HSP90 antibody was shown in the cytoplasm of primordial germ cells (PGCs). Other somatic cells in the gonad showed only faint reactivity. During testicular development, strong immunostaining was observed in the cytoplasm of embryonic germ cells and in spermatogonia and spermatocytes of the pre-pubertal testis. In adult testis reactivity of spermatogonia and pachytene spermatocytes was strong but reactivity of post-meiotic spermatogenic cells, i.e., secondary spermatocytes and spermatids, was extremely reduced. During ovarian development, immunostaining was also observed in the oogonia and the oocytes of pre-pubertal ovary. However, the staining of oocytes was reduced with the development of primordial follicles during the first week after birth. This study revealed that HSP90 is highly expressed in PGCs and continues to be expressed in both male and female pre-meiotic germ cells. The HSP90 accumulation may be essential for both male and female mammalian pre-meiotic germ cells.

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