Continuous ion separation via electrokinetic-driven ion migration path differentiation: Practical application to lithium extraction from brines

The effective separation of Mg2+ from Li+ is the key for realizing efficient and economic lithium extraction from brine, but remains challenging due to their similar chemical properties. Recent approaches...

Nuclear lamins promote protrusion dynamics and collective, confined migration in vivo

Cells migrate collectively through confined environments during development and cancer metastasis. While the nucleus, a large and stiff organelle, impedes cell migration between non-deformable pillars in vitro, its function in vivo may vary depending on the microenvironment. Further, it is unknown how nuclei contribute to collective migration in vivo and whether nuclei in different positions within cell collectives experience different forces. Here, we use border cell migration in the fly ovary as an in vivo model to investigate the effects of confined, collective migration on nuclei and the contribution of nuclear lamins to migration. We found severe yet transient nuclear deformations occur, particularly in the leading cell, as border cells squeeze through tiny crevices between germline cells, termed nurse cells. Leading cells extend protrusions between nurse cells, which may pry open space to allow the cluster to advance. Here we report that the leading cell nuclei deformed as they moved into leading protrusions. Then as protrusions widened, the nucleus recovered a more circular shape. These data suggest that lead cell nuclei may help protrusions expand and thereby enlarge the migration path. To test how nuclei might promote or impede border cell migration, we investigated nuclear lamins, proteins that assemble into intermediate filaments and structurally support the nuclear envelope. Depletion of the Drosophila B-type lamin, Lam, from the outer, motile border cells, but not the inner, nonmotile polar cells, impeded border cell migration, whereas perturbations of the A-type lamin, LamC, did not. While wild type border cell clusters typically have one large leading protrusion as they delaminate from the anterior follicular epithelium, clusters depleted of B-type lamin had multiple, short-lived protrusions, resulting in unproductive cluster movement and failure to progress along the migration path. Further, border cell nuclei depleted of B-type lamins were small, formed blebs, and ruptured. Together, these data indicate that B-type lamin is requied for nuclear integrity, which in turn stabilizes the leading protrusion and promotes overall cluster polarization and collective movement through confined spaces.

Promises are made to be broken: migrating R to strict semantics

Function calls in the R language do not evaluate their arguments, these are passed to the callee as suspended computations and evaluated if needed. After 25 years of experience with the language, there are very few cases where programmers leverage delayed evaluation intentionally and laziness comes at a price in performance and complexity. This paper explores how to evolve the semantics of a lazy language towards strictness-by-default and laziness-on-demand. To provide a migration path, it is necessary to provide tooling for developers to migrate libraries without introducing errors. This paper reports on a dynamic analysis that infers strictness signatures for functions to capture both intentional and accidental laziness. Over 99% of the inferred signatures were correct when tested against clients of the libraries.

Role of Gravitational Processes in the Migration of Heavy Metals in Soils of the Priolkhonye Mountain-Steppe Landscapes, Lake Baikal: Methodology of Research

The study object of this work is the soils of the Priolkhonye mountain-steppe landscapes. This research is performed at the Priolkhonye test site. Annual monitoring for exogenous geological process activity is conducted there. This research aims to study the influence of gravitational geological processes (landslides) on the migration of heavy metals, which are introduced into soil as a result of human agricultural activity. A methodology for the geoecological monitoring of soils based on an assessment of their contamination level by anthropogenic heavy metals, and a transfer mechanism throughout whole migration path (field–landslide–coast) is proposed. The following steps were taken to solve this problem: isolating a fine-grained fraction (<100 μm); determining the Hg, Cd, Pb, and Cu concentrations using wavelength dispersive X-ray fluorescence and atomic absorption spectroscopies, as well as total organic carbon (TOC) content using UV-Vis spectrophotometry; computing various geochemical indices; and describing the migration path of these heavy metals and TOC. The monitoring results showed that these pollutants migrate to the coastal zone as a result of landslides activation. The soil contamination level is increased which can cause negative toxic effects in coastal ecosystems, and could rise to a dangerous level for humans. Thus, the observed changes in the indices and TOC values allow us to assess the geoecological state of the study area; namely, the periods of landslide activity and the supply of these pollutants to the coastal zone.

Importance of 3-Way Coupled Modelling for Carbon Dioxide Sequestration in Depleted Reservoir

Carbon sequestration is the process of capturing and storage of atmospheric carbon dioxide. The objective of any carbon sequestration project is to store CO2 safely for hundreds or thousands of years with a goal of reducing global climate change. A depleted hydrocarbon reservoir is one of the potential storage sites being considered for long-term CO2 storage. The dynamic, geochemical, and geomechanics changes that occur during CO2 injection are inter-related. For example, when injected CO2 causes dissolution of reservoir rock, on one hand, porosity increases while rock strength decreases. On the other hand, reduced rock strength could cause additional compaction thus reducing porosity, whereas increase in pressure due to injection could cause dilation. Hence, it is critical to have an integrated model that captures effect of all changes on the storage capacity and integrity of the reservoir. Three major depleted gas reservoirs in Central Luconia field, located offshore Sarawak, are being evaluated for future CO2 storage. A 3-way coupled modelling approach that integrates dynamic model, geochemistry model, and geomechanics model is utilized to obtain cumulative effect of all three changes. This integrated model provides a more accurate estimate of 1) CO2 storage capacity, 2) Caprock integrity evaluation, 3) CO2 plume migration path, and 4) Volume of CO2 stored through different storage mechanisms (viz. hydrodynamic trapping, capillary trapping, solubility trapping, and mineral trapping). Apart from providing storage capacity, this model also provides inputs for evaluating integrity of caprock, fault reactivation study, MMV (Measurement, Monitoring, and Verification) planning, and estimating potential leak rates through plugged and abandoned wells. Using a 3-way coupled model, it is estimated that there is an average reduction in porosity of 5-10% (of initial porosity). This translates to an equivalent reduction in CO2 storage capacity of 5-10% compared to dynamic model. It is observed that pore collapse as a result of pressure depletion is primarily responsible for this reduction in porosity. It has also been observed that the injection can be continued till initial reservoir pressure is reached without breaching caprock integrity. CO2 plume migration path significantly affects MMV planning. Potential leak rate estimation is critical in mitigation and contingency planning.

Endothelial Focal Adhesions Are Functional Obstacles for Leukocytes During Basolateral Crawling

An inflammatory response requires leukocytes to migrate from the circulation across the vascular lining into the tissue to clear the invading pathogen. Whereas a lot of attention is focused on how leukocytes make their way through the endothelial monolayer, it is less clear how leukocytes migrate underneath the endothelium before they enter the tissue. Upon finalization of the diapedesis step, leukocytes reside in the subendothelial space and encounter endothelial focal adhesions. Using TIRF microscopy, we show that neutrophils navigate around these focal adhesions. Neutrophils recognize focal adhesions as physical obstacles and deform to get around them. Increasing the number of focal adhesions by silencing the small GTPase RhoJ slows down basolateral crawling of neutrophils. However, apical crawling and diapedesis itself are not affected by RhoJ depletion. Increasing the number of focal adhesions drastically by expressing the Rac1 GEF Tiam1 make neutrophils to avoid migrating underneath these Tiam1-expressing endothelial cells. Together, our results show that focal adhesions mark the basolateral migration path of neutrophils.