Heat-Shock Induction of Ionizing Radiation Resistance in Saccharomyces cerevisiae, and Correlation with Stationary Growth Phase

1982 ◽  
Vol 90 (2) ◽  
pp. 284 ◽  
Author(s):  
R. E. J. Mitchel ◽  
D. P. Morrison
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Heat Shock ◽  
Ionizing Radiation ◽  
Growth Phase ◽  
Radiation Resistance ◽  
Stationary Growth Phase ◽  
Stationary Growth ◽  
Ionizing Radiation Resistance ◽  
Heat Shock Induction

Induction of Hsp104 synthesis in Saccharomyces cerevisiae in the stationary growth phase is inhibited by the petite mutation

2014 ◽  
Vol 50 (3) ◽  
pp. 237-244 ◽  
Author(s):  
I. V. Fedoseeva ◽  
E. G. Rikhvanov ◽  
N. N. Varakina ◽  
T. M. Rusaleva ◽  
D. V. Pyatrikas ◽  
...  
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Growth Phase ◽  
Stationary Growth Phase ◽  
Stationary Growth ◽  
Petite Mutation

Occurrence of “Stress"-Proteins in Yeast after Heat-Shock, Acrylonitrile Treatment and during the Stationary Growth Phase

1985 ◽  
Vol 40 (1-2) ◽  
pp. 26-28 ◽  
Author(s):  
Ulrich Pfeffer ◽  
Bernd Schulz-Harder
Keyword(s):  
Heat Shock ◽  
Growth Phase ◽  
Exponential Growth ◽  
Stress Proteins ◽  
Stationary Growth Phase ◽  
Yeast Cells ◽  
Exponential Growth Phase ◽  
Stationary Growth ◽  
Molecular Weights

The response of yeast cells to different kinds of “stress” is not identical. Cells of the stationary growth phase synthesize three new proteins of molecular weights 68, 27 and 24 kD, compared with cells of the exponential growth phase, while heat-shocked cells exhibit new proteins of 100, 90. 84, 70 and 24 kD. After treatment with acrylonitrile two new proteins with molecular weights of 70 and 46 kD appear. However, all three kinds of “stress” lead to the induction of a ribonuclease.

scholarly journals Conversion of Daidzein and Genistein by an Anaerobic Bacterium Newly Isolated from the Mouse Intestine

2008 ◽  
Vol 74 (15) ◽  
pp. 4847-4852 ◽  
Author(s):  
Anastasia Matthies ◽  
Thomas Clavel ◽  
Michael Gütschow ◽  
Wolfram Engst ◽  
Dirk Haller ◽  
...  
Keyword(s):  
Stationary Phase ◽  
Enzyme Induction ◽  
Growth Phase ◽  
Anaerobic Bacterium ◽  
Stationary Growth Phase ◽  
Gut Bacteria ◽  
Soy Isoflavones ◽  
Strictly Anaerobic ◽  
Stationary Growth ◽  
Mouse Intestine

ABSTRACT The metabolism of isoflavones by gut bacteria plays a key role in the availability and bioactivation of these compounds in the intestine. Daidzein and genistein are the most common dietary soy isoflavones. While daidzein conversion yielding equol has been known for some time, the corresponding formation of 5-hydroxy-equol from genistein has not been reported previously. We isolated a strictly anaerobic bacterium (Mt1B8) from the mouse intestine which converted daidzein via dihydrodaidzein to equol as well as genistein via dihydrogenistein to 5-hydroxy-equol. Strain Mt1B8 was a gram-positive, rod-shaped bacterium identified as a member of the Coriobacteriaceae. Strain Mt1B8 also transformed dihydrodaidzein and dihydrogenistein to equol and 5-hydroxy-equol, respectively. The conversion of daidzein, genistein, dihydrodaidzein, and dihydrogenistein in the stationary growth phase depended on preincubation with the corresponding isoflavonoid, indicating enzyme induction. Moreover, dihydrogenistein was transformed even more rapidly in the stationary phase when strain Mt1B8 was grown on either genistein or daidzein. Growing the cells on daidzein also enabled conversion of genistein. This suggests that the same enzymes are involved in the conversion of the two isoflavones.

scholarly journals The extracellular proteome of Rhizobium etli CE3 in exponential and stationary growth phase

2010 ◽  
Vol 8 (1) ◽  
pp. 51 ◽  
Author(s):  
Niurka Meneses ◽  
Guillermo Mendoza-Hernández ◽  
Sergio Encarnación
Keyword(s):  
Growth Phase ◽  
Stationary Growth Phase ◽  
Rhizobium Etli ◽  
Stationary Growth ◽  
Extracellular Proteome

scholarly journals Directed Evolution of Ionizing Radiation Resistance in Escherichia coli

2009 ◽  
Vol 191 (16) ◽  
pp. 5240-5252 ◽  
Author(s):  
Dennis R. Harris ◽  
Steve V. Pollock ◽  
Elizabeth A. Wood ◽  
Reece J. Goiffon ◽  
Audrey J. Klingele ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Ionizing Radiation ◽  
Directed Evolution ◽  
Radiation Resistance ◽  
Bacterial Species ◽  
Repair System ◽  
Multiple Alleles ◽  
Evolutionary Pathways ◽  
K 12 ◽  
Ionizing Radiation Resistance

ABSTRACT We have generated extreme ionizing radiation resistance in a relatively sensitive bacterial species, Escherichia coli, by directed evolution. Four populations of Escherichia coli K-12 were derived independently from strain MG1655, with each specifically adapted to survive exposure to high doses of ionizing radiation. D37 values for strains isolated from two of the populations approached that exhibited by Deinococcus radiodurans. Complete genomic sequencing was carried out on nine purified strains derived from these populations. Clear mutational patterns were observed that both pointed to key underlying mechanisms and guided further characterization of the strains. In these evolved populations, passive genomic protection is not in evidence. Instead, enhanced recombinational DNA repair makes a prominent but probably not exclusive contribution to genome reconstitution. Multiple genes, multiple alleles of some genes, multiple mechanisms, and multiple evolutionary pathways all play a role in the evolutionary acquisition of extreme radiation resistance. Several mutations in the recA gene and a deletion of the e14 prophage both demonstrably contribute to and partially explain the new phenotype. Mutations in additional components of the bacterial recombinational repair system and the replication restart primosome are also prominent, as are mutations in genes involved in cell division, protein turnover, and glutamate transport. At least some evolutionary pathways to extreme radiation resistance are constrained by the temporally ordered appearance of specific alleles.

Bacterial sensitivity to UV light as a model for ionizing radiation resistance

1993 ◽  
Vol 18 (2) ◽  
pp. 127-136 ◽  
Author(s):  
Andrew A. Arrange ◽  
Tommy J. Phelps ◽  
Robert E. Benoit ◽  
Anthony V. Palumbo ◽  
David C. White
Keyword(s):  
Ionizing Radiation ◽  
Radiation Resistance ◽  
Uv Light ◽  
Bacterial Sensitivity ◽  
Ionizing Radiation Resistance

scholarly journals Proteomic Study of the Exponential-Stationary Growth Phase Transition in the Haloarchaea Natrialba magadii and Haloferax volcanii

PROTEOMICS ◽  
2018 ◽  
Vol 18 (14) ◽  
pp. 1800116 ◽  
Author(s):  
Micaela Cerletti ◽  
María Ines Giménez ◽  
Christian Tröetschel ◽  
Celeste D’ Alessandro ◽  
Ansgar Poetsch ◽  
...  
Keyword(s):  
Phase Transition ◽  
Growth Phase ◽  
Stationary Growth Phase ◽  
Haloferax Volcanii ◽  
Stationary Growth ◽  
Proteomic Study ◽  
Natrialba Magadii ◽  
Growth Phase Transition

Effect of Temperature on Growth and Alpha Toxin Production by Clostridium perfringens1

1979 ◽  
Vol 42 (11) ◽  
pp. 848-851 ◽  
Author(s):  
Y. PARK ◽  
E. M. MIKOLAJCIK
Keyword(s):  
Growth Phase ◽  
Incubation Temperature ◽  
Ground Beef ◽  
Population Level ◽  
Toxin Production ◽  
Stationary Growth Phase ◽  
Effect Of Temperature ◽  
Alpha Toxin ◽  
Stationary Growth ◽  
Incubation Temperatures

Growth and alpha toxin production by a strain of Clostridium perfringens was determined in Thioglycollate medium, beef broth with ground beef, and beef broth with ground beef and soy protein. Incubation temperatures ranged from 15 to 50 C. In Thioglycollate medium, maximum alpha toxin production occurred at 35 C and was 40 times greater than that observed at 45 C. However, generation time and maximum population were approximately the same at 35 and 45 C. At 15 C, a two log cycle reduction in viable counts occurred within 6 h. Irrespective of incubation temperature, alpha toxin levels in Thioglycollate medium declined as the incubation period was extended beyond the stationary growth phase. In the beef broth with ground beef system which was studied at 35 C only, the organism grew slower and produced less toxin than in Thioglycollate medium. The amount of alpha toxin detected was influenced to a greater extent by the incubation time and temperature, the holding time beyond the stationary growth phase, and the growth medium than by the population level of C. perfringens.

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