208 PROLIFERATION AND DIFFERENTIATION OF PREOSTEOBLASTS IN AN IN VITRO MODEL OF DISTRACTION OSTEOGENESIS

2005 ◽  
Vol 53 (1) ◽  
pp. S114.6-S114
Author(s):  
O. C. Constantinescu ◽  
J. S. Gabbay ◽  
C. O'Hara ◽  
A. Tahernia ◽  
S. A. Mitchell ◽  
...  
Keyword(s):  
Distraction Osteogenesis ◽  
In Vitro Model ◽  
Proliferation And Differentiation ◽  
Vitro Model

An In Vitro Model for Dissecting Distraction Osteogenesis

2005 ◽  
Vol 16 (1) ◽  
pp. 71-78 ◽  
Author(s):  
Francesco Carinci ◽  
Furio Pezzetti ◽  
Anna Maria Spina ◽  
Annalisa Palmieri ◽  
Friedrick Carls ◽  
...  
Keyword(s):  
Distraction Osteogenesis ◽  
In Vitro Model ◽  
Vitro Model

Bone cell stretching as an in-vitro model for distraction osteogenesis

1997 ◽  
Vol 26 ◽  
pp. 74
Author(s):  
U. Meyer ◽  
J. Piffko ◽  
J. Kleinheinz ◽  
D.H. Szulczewski ◽  
D.B. Jones ◽  
...  
Keyword(s):  
Distraction Osteogenesis ◽  
In Vitro Model ◽  
Bone Cell ◽  
Cell Stretching ◽  
Vitro Model

Acid modulation of HT29 cell growth and differentiation. An in vitro model for Barrett's esophagus

1997 ◽  
Vol 110 (5) ◽  
pp. 663-671 ◽  
Author(s):  
R.C. Fitzgerald ◽  
M.B. Omary ◽  
G. Triadafilopoulos
Keyword(s):  
Cell Proliferation ◽  
Barrett’S Esophagus ◽  
Barrett's Esophagus ◽  
In Vitro Model ◽  
Acid Exposure ◽  
Ht29 Cells ◽  
Cell Proliferation And Differentiation ◽  
Proliferation And Differentiation ◽  
Vitro Model

Cell proliferation and differentiation are influenced by environmental factors, including the extracellular pH. We recently showed, using an ex vivo organ culture system of human mucosal Barrett's esophageal biopsies, that acid has a highly variable effect on cell proliferation and differentiation depending on the pattern of acid exposure. Study of the mechanisms underlying these dynamic effects of acid on this premalignant intestinal-like epithelium is hampered by lack of an immortalized cell line. We therefore investigated the effect of acid exposure on the human colonic carcinoma cell line HT29, chosen because of its intestinal cell derivation and its ability to differentiate in vitro. HT29 cells exposed to pH 5 medium either continuously (up to 3 weeks), or as a short (1 hour) pulse, were compared with cells cultured at pH 7.4. Villin expression was induced only by long term acid exposure, and correlated with the development of differentiated polarized cells that contain a brush border and microvillus inclusions. Chronic acid exposure arrested cell proliferation, whereas a 1 hour acid-pulse enhanced cell proliferation, as determined by [3H]thymidine incorporation assays and proliferating cell nuclear antigen expression. Serum starvation attenuated the hyperproliferative effect of an acid-pulse. In addition, the doubling time of at least the first cell cycle after an acid-pulse was shortened. The Na/H exchanger is likely to play a role since the hyperproliferative acid-induced response was blocked by amiloride; and the activity of the exchanger was increased at acidic pH as determined by 22Na uptake. These results support a role for extracellular pH on cell proliferation and differentiation of HT29 cells. Furthermore, these findings parallel the dynamic effects of acid on Barrett's esophagus, and suggest that HT29 cells could serve as an in vitro model for studying the mechanism of acid modulation in Barrett's esophagus.

An in vitro model for investigation of vitamin A effects on wound healing

2021 ◽  
pp. 1-6
Author(s):  
Hoda Keshmiri Neghab ◽  
Mohammad Hasan Soheilifar ◽  
Gholamreza Esmaeeli Djavid
Keyword(s):  
Wound Healing ◽  
Endothelial Cell ◽  
Vitamin A ◽  
In Vitro Model ◽  
Cellular Proliferation ◽  
Endothelial Cell Migration ◽  
Normal Fibroblast ◽  
Anti Inflammatory ◽  
Vitro Model

Abstract. Wound healing consists of a series of highly orderly overlapping processes characterized by hemostasis, inflammation, proliferation, and remodeling. Prolongation or interruption in each phase can lead to delayed wound healing or a non-healing chronic wound. Vitamin A is a crucial nutrient that is most beneficial for the health of the skin. The present study was undertaken to determine the effect of vitamin A on regeneration, angiogenesis, and inflammation characteristics in an in vitro model system during wound healing. For this purpose, mouse skin normal fibroblast (L929), human umbilical vein endothelial cell (HUVEC), and monocyte/macrophage-like cell line (RAW 264.7) were considered to evaluate proliferation, angiogenesis, and anti-inflammatory responses, respectively. Vitamin A (0.1–5 μM) increased cellular proliferation of L929 and HUVEC (p < 0.05). Similarly, it stimulated angiogenesis by promoting endothelial cell migration up to approximately 4 fold and interestingly tube formation up to 8.5 fold (p < 0.01). Furthermore, vitamin A treatment was shown to decrease the level of nitric oxide production in a dose-dependent effect (p < 0.05), exhibiting the anti-inflammatory property of vitamin A in accelerating wound healing. These results may reveal the therapeutic potential of vitamin A in diabetic wound healing by stimulating regeneration, angiogenesis, and anti-inflammation responses.

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