Spiradenocarcinoma Arising from a Spiradenocylindroma: Unusual Case with Lymphoepithelioma-Like Areas

Background: Hybrid skin adnexal tumors are common, and spiradenocylindroma is well described. Objective: However, malignant transformation in this setting is infrequent, especially resemblance to lymphoepithelioma-like carcinoma of skin, which is not associated with Epstein-Barr virus. Methods: A 65-year-old female presented with ataxia and a skin nodule composed of a hybrid adnexal tumor (spiradenoma and cylindroma) that transitioned into an undifferentiated carcinoma with attendant lymphocytes and plasma cells. There was widespread dissemination of the undifferentiated component to regional neck lymph nodes. Results: The undifferentiated component resembled a lymphoepithelioma-like carcinoma and showed focal evidence of tubular and squamous differentiation. The tumor was Epstein-Barr encoded RNAs (EBER) negative by in situ hybridization. No evidence of neuroendocrine differentiation was seen in the tumor, despite the patient having symptoms of paraneoplastic ataxia that improved after surgery. Conclusion: This case highlights the transition of a benign hybrid tumor (spiradenocylindroma) into a spiradenocarcinoma that resembled lymphoepithelioma-like carcinoma of skin. It also highlights two unusual features: widespread lymph node dissemination and presentation with paraneoplastic syndrome-associated ataxia.

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High expression of the bcl-x gene in Reed-Sternberg cells of Hodgkin's disease

Blood ◽

10.1182/blood.v85.10.2671.bloodjournal85102671 ◽

1995 ◽

Vol 85 (10) ◽

pp. 2671-2674 ◽

Cited By ~ 45

Author(s):

D Schlaifer ◽

M March ◽

S Krajewski ◽

G Laurent ◽

J Pris ◽

...

Keyword(s):

Hodgkin's Disease ◽

Plasma Cells ◽

Hodgkin’S Disease ◽

Protein A ◽

Mixed Cellularity ◽

Barr Virus ◽

X Gene ◽

Epstein Barr ◽

Apoptosis Regulation

The expression of bcl-x protein, a bcl-2-related protein present in cortical thymocytes, activated lymphocytes, and plasma cells of reactive lymph nodes, was investigated in 44 cases of Hodgkin's disease (HD) in parallel with bcl-2 and Epstein-Barr virus (EBV) status. Eighty-six percent of the cases were positive for bcl-x, among them 27% with a strong signal in more than 75% of the Reed-Sternberg cells. Positivity for bcl-x was found in, respectively, 100% and 92% of the nodular sclerosis and mixed cellularity subtypes, although 4 cases of lymphocyte predominance subtype were negative. This finding was in contrast with the weaker positivity for bcl-2 staining in 44% of the cases. EBV small RNAs were detected in 43% of the cases by using in situ hybridization. Of interest, 100% of the EBV-positive samples were positive for bcl-x, whereas only 38% of these cases were bcl-2 positive. Our findings show that the bcl-x gene expression is high in HD, suggesting that bcl-x may have a role in the pathogenesis of at least some cases of HD via apoptosis regulation.

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Detection of Epstein-Barr virus genome in sinonasal undifferentiated carcinoma by use of in situ hybridization

Otolaryngology ◽

10.1016/s0194-5998(95)70172-9 ◽

1995 ◽

Vol 112 (6) ◽

pp. 659-664 ◽

Cited By ~ 22

Author(s):

O GALLO ◽

S DILOLLO ◽

E GALLINA ◽

G BARONI

Keyword(s):

In Situ Hybridization ◽

Epstein Barr Virus ◽

Virus Genome ◽

Undifferentiated Carcinoma ◽

Sinonasal Undifferentiated Carcinoma ◽

Barr Virus ◽

Epstein Barr

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Absence of Epstein-Barr virus encoded RNA and latent membrane protein (LMP1) in salivary gland neoplasms

The Journal of Laryngology & Otology ◽

10.1017/s0022215100145542 ◽

1999 ◽

Vol 113 (10) ◽

pp. 906-908 ◽

Cited By ~ 9

Author(s):

A. M. Pollock ◽

M. Toner ◽

M. McMenamin ◽

J. Walker ◽

C. I. Timon

Keyword(s):

Salivary Gland ◽

Membrane Protein ◽

Epstein Barr Virus ◽

Undifferentiated Carcinoma ◽

Latent Membrane Protein ◽

Positive Signal ◽

Salivary Gland Tumours ◽

Barr Virus ◽

Epstein Barr

AbstractA series of 55 (42 benign and 13 malignant) salivary gland tumours were investigated by immunohistochemistry, to detect Epstein-Barr virus (EBV) latent membrane protein (LMP1) and byin situhybridization for EBV-encoded RNA. Non-neoplastic gland from all the patients with tumours and 15 control glands were also examined. All cases, both neoplastic and non-neoplastic were negative for LMP1 and failed to show any positive signal byin situhybridization for EBV RNA. One undifferentiated carcinoma from a European patient was included in the group. These results confirm previous reports of an ethnic association between EBV and undifferentiated carcinomas of the salivary gland. They do not support an aetiological role for EBV in other salivary gland tumours.

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Undifferentiated carcinoma of the parotid gland in a white patient: Detection of Epstein-Barr virus by in situ hybridization

Human Pathology ◽

10.1016/s0046-8177(96)90144-6 ◽

1996 ◽

Vol 27 (1) ◽

pp. 87-90 ◽

Cited By ~ 28

Author(s):

Angeliki Kotsianti ◽

John Costopoulos ◽

Susan Morgello ◽

Constantine Papadimitriou

Keyword(s):

In Situ Hybridization ◽

Parotid Gland ◽

Epstein Barr Virus ◽

Undifferentiated Carcinoma ◽

White Patient ◽

Barr Virus ◽

Epstein Barr

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Epstein-Barr Virus RNA Detection and Glandular Differentiation in Nasopharyngeal Carcinoma

Archives of Pathology & Laboratory Medicine ◽

10.5858/2000-124-1369-ebvrda ◽

2000 ◽

Vol 124 (9) ◽

pp. 1369-1372

Author(s):

Dhanpat Jain ◽

Vinita Parkash ◽

Maomi Li ◽

James Gill ◽

Jill Crouch ◽

...

Keyword(s):

Epstein Barr Virus ◽

Virus Genome ◽

Undifferentiated Carcinoma ◽

Latent Membrane Protein ◽

Rna Detection ◽

Barr Virus ◽

Virus Rna ◽

Epstein Barr ◽

Virus Latent Membrane Protein

Abstract Most tumors arising in the nasopharynx are either squamous cell carcinoma or so-called undifferentiated carcinoma of the nasopharyngeal type. Primary adenocarcinomas of the nasopharynx are rare, and glandular differentiation in undifferentiated carcinoma of the nasopharyngeal type has not been reported to date. We report 2 cases of undifferentiated carcinoma of the nasopharyngeal type that show distinct glandular differentiation by light microscopy, histochemistry, immunohistochemistry, and ultrastructure. Both tumors showed equal positivity for Epstein-Barr virus latent membrane protein and in situ hybridization for Epstein-Barr virus genome in the undifferentiated areas of the tumor and those featuring glandular differentiation.

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Molecular signature of Epstein-Barr virus infection in MS brain lesions

Neurology Neuroimmunology & Neuroinflammation ◽

10.1212/nxi.0000000000000466 ◽

2018 ◽

Vol 5 (4) ◽

pp. e466 ◽

Cited By ~ 24

Author(s):

Monica A. Moreno ◽

Noga Or-Geva ◽

Blake T. Aftab ◽

Rajiv Khanna ◽

Ed Croze ◽

...

Keyword(s):

In Situ Hybridization ◽

Brain Tissue ◽

Epstein Barr Virus ◽

Plasma Cells ◽

Brain Lesions ◽

Molecular Signature ◽

Epstein Barr Virus Infection ◽

Barr Virus ◽

Epstein Barr

ObjectiveWe sought to confirm the presence and frequency of B cells and Epstein-Barr virus (EBV) (latent and lytic phase) antigens in archived MS and non-MS brain tissue by immunohistochemistry.MethodsWe quantified the type and location of B-cell subsets within active and chronic MS brain lesions in relation to viral antigen expression. The presence of EBV-infected cells was further confirmed by in situ hybridization to detect the EBV RNA transcript, EBV-encoded RNA-1 (EBER-1).ResultsWe report the presence of EBV latent membrane protein 1 (LMP-1) in 93% of MS and 78% of control brains, with a greater percentage of MS brains containing CD138+ plasma cells and LMP-1–rich populations. Notably, 78% of chronic MS lesions and 33.3% of non-MS brains contained parenchymal CD138+ plasma cells. EBV early lytic protein, EBV immediate-early lytic gene (BZLF1), was also observed in 46% of MS, primarily in association with chronic lesions and 44% of non-MS brain tissue. Furthermore, 85% of MS brains revealed frequent EBER-positive cells, whereas non-MS brains seldom contained EBER-positive cells. EBV infection was detectable, by immunohistochemistry and by in situ hybridization, in both MS and non-MS brains, although latent virus was more prevalent in MS brains, while lytic virus was restricted to chronic MS lesions.ConclusionsTogether, our observations suggest an uncharacterized link between the EBV virus life cycle and MS pathogenesis.

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