Detection of Epstein-Barr virus transcripts in chemically or immunologically-activated cells and in a null cell-line (HLN-STL-C) by in situ hybridization with alkaline phosphatase-linked oligonucleotide probes

1993 ◽  
Vol 44 (2-3) ◽  
pp. 141-154 ◽  
Author(s):  
Takashi Hironaka ◽  
Makoto Nagasaki ◽  
Shigeru Morikawa ◽  
Kanji Hirai
Keyword(s):  
Alkaline Phosphatase ◽  
In Situ Hybridization ◽  
Cell Line ◽  
Epstein Barr Virus ◽  
Oligonucleotide Probes ◽  
Null Cell ◽  
Barr Virus ◽  
Epstein Barr

scholarly journals Epstein-Barr virus-associated gastric carcinoma in Brazil: comparison between in situ hybridization and polymerase chain reaction detection

2012 ◽  
Vol 43 (1) ◽  
pp. 393-404 ◽  
Author(s):  
Marcos Antonio Pereira de Lima ◽  
Márcia Valéria Pitombeira Ferreira ◽  
Marcos Aurélio Pessoa Barros ◽  
Maria Inês de Moura Campos Pardini ◽  
Adriana Camargo Ferrasi ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
In Situ Hybridization ◽  
Gastric Carcinoma ◽  
Epstein Barr Virus ◽  
Polymerase Chain Reaction Detection ◽  
Chain Reaction ◽  
Barr Virus ◽  
Polymerase Chain ◽  
Epstein Barr

The impact of Epstein-Barr virus status on clinical outcome in diffuse large B-cell lymphoma

Blood ◽  
2007 ◽  
Vol 110 (3) ◽  
pp. 972-978 ◽  
Author(s):  
Sarah Park ◽  
Jeeyun Lee ◽  
Young Hyeh Ko ◽  
Arum Han ◽  
Hyun Jung Jun ◽  
...  
Keyword(s):  
In Situ Hybridization ◽  
B Cell ◽  
Epstein Barr Virus ◽  
Progression Free Survival ◽  
B Cell Lymphoma ◽  
Free Survival ◽  
Barr Virus ◽  
Large B Cell Lymphoma ◽  
Epstein Barr

AbstractTo define prognostic impact of Epstein-Barr virus (EBV) infection in diffuse large B-cell lymphoma (DLBCL), we investigated EBV status in patients with DLBCL. In all, 380 slides from paraffin-embedded tissue were available for analysis by EBV-encoded RNA-1 (EBER) in situ hybridization, and 34 cases (9.0%) were identified as EBER-positive. EBER positivity was significantly associated with age greater than 60 years (P = .005), more advanced stage (P < .001), more than one extranodal involvement (P = .009), higher International Prognostic Index (IPI) risk group (P = .015), presence of B symptom (P = .004), and poorer outcome to initial treatment (P = .006). The EBER+ patients with DLBCL demonstrated substantially poorer overall survival (EBER+ vs EBER− 35.8 months [95% confidence interval (CI), 0-114.1 months] vs not reached, P = .026) and progression-free survival (EBER+ vs EBER− 12.8 months [95% CI, 0-31.8 months] vs 35.8 months [95% CI, 0-114.1 months], respectively (P = .018). In nongerminal center B-cell–like subtype, EBER in situ hybridization positivity retained its statistical significance at the multivariate level (P = .045). Nongerminal center B-cell–like patients with DLBCL with EBER positivity showed substantially poorer overall survival with 2.9-fold (95% CI, 1.1-8.1) risk for death. Taken together, DLBCL patients with EBER in situ hybridization+ pursued more rapidly deteriorating clinical course with poorer treatment response, survival, and progression-free survival.

Detection of Epstein–Barr virus in children's adenoids by in situ hybridization

2003 ◽  
Vol 1257 ◽  
pp. 157-160 ◽  
Author(s):  
L.H. Endo ◽  
E. Sakano ◽  
L.A. Camargo ◽  
D.R. Ferreira ◽  
G.A. Pinto ◽  
...  
Keyword(s):  
In Situ Hybridization ◽  
Epstein Barr Virus ◽  
Barr Virus ◽  
Epstein Barr

Nasal Natural Killer Cell/T-Cell Lymphoma Showing Cellular Morphology Mimicking Normal Lymphocytes

2002 ◽  
Vol 126 (5) ◽  
pp. 602-605
Author(s):  
Katsuya Chinen ◽  
Yasuhiko Kaneko ◽  
Toshiyuki Izumo ◽  
Yasuo Ohkura ◽  
Osamu Matsubara ◽  
...  
Keyword(s):  
In Situ Hybridization ◽  
T Cell ◽  
Natural Killer ◽  
Epstein Barr Virus ◽  
Cell Lymphoma ◽  
Normal Lymphocyte ◽  
Lymphoma Cells ◽  
Barr Virus ◽  
Epstein Barr

Abstract We report the autopsy case of a 34-year-old Japanese man with a nasal natural killer (NK)-cell/T-cell lymphoma. The patient developed the disease at 32 years of age, and a biopsy of the nasopharynx revealed pleomorphic lymphoma cell proliferation. Radiotherapy was performed, but the patient eventually died of respiratory failure. After radiotherapy, no histologic evidence of malignancy was obtained with biopsy materials featuring lymphocytic infiltration. Autopsy studies, including in situ hybridization for Epstein-Barr virus–encoded RNA, revealed generalized infiltration of normal lymphocyte-like, UCHL-1–positive, and Epstein-Barr virus–encoded RNA–positive lymphoma cells. Monoclonal proliferation of the Epstein-Barr virus–carrying cells was verified by means of Southern blot analysis. Retrospectively, we concluded that the normal lymphocyte-like presentation of the lymphoma cells, probably influenced by radiotherapy, prevented pathologists from recognizing the lymphoma. The utility of in situ hybridization for Epstein-Barr virus–encoded RNA in identification of tumor cells is emphasized with respect to the present case.

scholarly journals In situ hybridization for the detection of Epstein–Barr virus in central nervous system lymphomas

Cancer ◽  
1991 ◽  
Vol 67 (2) ◽  
pp. 444-448 ◽  
Author(s):  
Raouf E. Nakhleh ◽  
J. Carlos Manivel ◽  
Cedith M. Copenhaver ◽  
Joo H. Sung ◽  
John G. Strickler
Keyword(s):  
Central Nervous System ◽  
Nervous System ◽  
In Situ Hybridization ◽  
Epstein Barr Virus ◽  
Barr Virus ◽  
Epstein Barr

scholarly journals Detection of Epstein-Barr Virus in 130 Cases of Eyelid Sebaceous Gland Carcinoma Using In Situ Hybridization

2020 ◽  
Vol 2020 ◽  
pp. 1-6
Author(s):  
Huanhuan Gao ◽  
Lijuan Tang ◽  
Jianxian Lin ◽  
Wenxin Zhang ◽  
Yongping Li ◽  
...  
Keyword(s):  
In Situ Hybridization ◽  
Sebaceous Gland ◽  
Epstein Barr Virus ◽  
Clinicopathologic Features ◽  
Tumor Differentiation ◽  
Sebaceous Gland Carcinoma ◽  
Barr Virus ◽  
Gland Carcinoma ◽  
Epstein Barr

Purpose. In this study, we aimed to investigate the presence of Epstein–Barr virus (EBV) in the eyelid sebaceous gland carcinoma (SGC) and its association with the clinicopathologic features. Methods. One hundred and thirty paraffin-embedded SGC specimens were retrieved from the Clinical Pathology Department of Zhongshan Ophthalmic Center. Epstein–Barr virus-encoded RNA (EBER) was detected with in situ hybridization (ISH) using the Leica BOND system autostainer. The age and gender distributions of all patients were analyzed and compared with earlier reports. Pearson’s χ2 and Fisher’s exact tests were used to determine the association between clinicopathological features such as age, gender, laterality eye, tumor basal dimension, degree of tumor differentiation, and EBER positivity. Likewise, the relationship between the grade and tumor basal dimension in EBER-positive SGC of the eyelid was analyzed. Results. Thirty-four out of one hundred and thirty (26.2%) eyelid SGC specimens were positively stained for EBER. The age range of highest incidence was 46–75 years, and the female to male ratio was 1 : 0.9. No significant correlation was found between EBER-positivity and age (p=0.5370), gender (p=0.4758), and degree of tumor differentiation (p=0.7787). However, EBV positivity was strongly correlated with the right eye (p=0.0287), the tumor basal dimension (p=0.0001). EBV positivity grade presented statistically associated with tumor size (p=0.0329). Conclusion. We conclude that ISH is a sensitive method to identify EBV in SGC of the eyelid. A possible causal association of EBV in SGC patients is suggested by high frequency of EBER-ISH positivity and its association with the clinicopathologic features.

scholarly journals Guidelines for Interpreting EBER In Situ Hybridization and LMP1 Immunohistochemical Tests for Detecting Epstein-Barr Virus in Hodgkin Lymphoma

2002 ◽  
Vol 117 (2) ◽  
pp. 259-267 ◽  
Author(s):  
Margaret L. Gulley ◽  
Sally L. Glaser ◽  
Fiona E. Craig ◽  
Michael Borowitz ◽  
Risa B. Mann ◽  
...  
Keyword(s):  
In Situ Hybridization ◽  
Hodgkin Lymphoma ◽  
Epstein Barr Virus ◽  
Barr Virus ◽  
Epstein Barr

Detection of occult nasopharyngeal primary tumours by means of in situ hybridization

1992 ◽  
Vol 106 (4) ◽  
pp. 345-348 ◽  
Author(s):  
R. Feinmesser ◽  
M. Feinmesser ◽  
J. L. Freeman ◽  
A. M. Noyek ◽  
N. Livni
Keyword(s):  
In Situ Hybridization ◽  
Epstein Barr Virus ◽  
Primary Lesion ◽  
Hybridization Technique ◽  
Neck Node ◽  
Barr Virus ◽  
Neck Metastases ◽  
Node Metastases ◽  
Epstein Barr

AbstractDetection of nasopharyngeal carcinoma primaries in patients presenting with neck node metastases may sometimes demand considerable efforts. By using the ‘ in situ hybridization’ technique, wemanage to identify the Epstein-Barr virus in neck metastases secondary to nasopharyngeal carcinomas. We propose that such identification in neck node metastases where the primary lesion is unknown indicates a nasopharyngeal primary.

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