scholarly journals Scanning electron microscopic observations of the enamel surface in deciduous molar tooth germ of human.

1985 ◽  
Vol 27 (3) ◽  
pp. 870-876
Author(s):  
Reiki Sukekawa ◽  
Shigehisa Yamamoto
2010 ◽  
Vol 35 (2) ◽  
pp. 169-172 ◽  
Author(s):  
Bhoomika Ahuja ◽  
Ramakrishna Yeluri ◽  
M Sudhindra Baliga ◽  
AK Munshi

Objectives: This study was undertaken to evaluate the topographical features of enamel surface deproteinized with sodium hypochlorite (NaOCl) and etched with phosphoric acid (H3PO4) compared to phosphoric acid alone using Scanning Electron Microscopic (SEM) Analysis. Study Design: 30 enamel blocks of 1mm2 from ten human sound extracted permanent molars were obtained and treated as under: Group 1 (10 blocks): Enamel surface was etched with 37% H3PO4 gel for 15 seconds. Group 2 (10 blocks):Enamel surface was treated with 5.25% NaOCl for 60 seconds and then etched with 37% H3PO4 gel for 15 seconds. 10 enamel blocks were included in the control group where no treatment was carried out. The samples were subjected to SEM analysis and 5 microphotographs of each sample were obtained at 500X magnification and evaluated for the quality of etching pattern of the enamel in percentage (%) using Auto-CAD 2007 software. Results: Mean values of etching pattern in Group 1 being 55.76% and Group 2 being 53.58%. No significant difference was observed between the two groups. Conclusion: The use of 37% phosphoric acid for 15 seconds still remains the best method for pretreatment of enamel.


1997 ◽  
Vol 11 (4) ◽  
pp. 239-243 ◽  
Author(s):  
Marcelo FAVA ◽  
Ii-Sei WATANABE ◽  
Flávio FAVA-DE-MORAES ◽  
Luciane Ribeiro de Rezende Sucasas da COSTA

The frequency, structure and thickness of the prismless enamel layer in the buccal and lingual surfaces of non-erupted deciduous molar teeth were described. The teeth were extracted, kept in a 70% ethanol solution, dried, coated with gold and examined in a scanning electron microscope JEOL, JSM-6.100. The aprismatic layer was observed in the occlusal, middle and cervical thirds of all buccal and lingual surfaces. The hydroxyapatite crystals were arranged parallel to each other and perpendicular to the enamel surface. No statistically significant differences were observed between the occlusal, middle and cervical thirds, which had 7.257 <FONT FACE="Symbol">m</FONT> m of average thickness.


Author(s):  
Toichiro Kuwabara

Although scanning electron microscopy has a great potential in biological application, there are certain limitations in visualization of the biological structure. Satisfactory techniques to demonstrate natural surfaces of the tissue and the cell have been reported by several investigators. However, it is commonly found that the surface cell membrane is covered with a minute amount of mucin, secretory substance or tissue fluid as physiological, pathological or artefactual condition. These substances give a false surface appearance, especially when the tissue is fixed with strong fixatives. It seems important to remove these coating substances from the surface of the cell for demonstration of the true structure.


Author(s):  
T. Kanetaka ◽  
M. Cho ◽  
S. Kawamura ◽  
T. Sado ◽  
K. Hara

The authors have investigated the dissolution process of human cholesterol gallstones using a scanning electron microscope(SEM). This study was carried out by comparing control gallstones incubated in beagle bile with gallstones obtained from patients who were treated with chenodeoxycholic acid(CDCA).The cholesterol gallstones for this study were obtained from 14 patients. Three control patients were treated without CDCA and eleven patients were treated with CDCA 300-600 mg/day for periods ranging from four to twenty five months. It was confirmed through chemical analysis that these gallstones contained more than 80% cholesterol in both the outer surface and the core.The specimen were obtained from the outer surface and the core of the gallstones. Each specimen was attached to alminum sheet and coated with carbon to 100Å thickness. The SEM observation was made by Hitachi S-550 with 20 kV acceleration voltage and with 60-20, 000X magnification.


Author(s):  
Loren Anderson ◽  
Pat Pizzo ◽  
Glen Haydon

Transmission electron microscopy of replicas has long been used to study the fracture surfaces of components which fail in service. Recently, the scanning electron microscope (SEM) has gained popularity because it allows direct examination of the fracture surface. However, the somewhat lower resolution of the SEM coupled with a restriction on the sample size has served to limit the use of this instrument in investigating in-service failures. It is the intent of this paper to show that scanning electron microscopic examination of conventional negative replicas can be a convenient and reliable technique for determining mode of failure.


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