Enamel Deproteinization before Acid Etching – A Scanning Electron Microscopic Observation

2010 ◽  
Vol 35 (2) ◽  
pp. 169-172 ◽  
Author(s):  
Bhoomika Ahuja ◽  
Ramakrishna Yeluri ◽  
M Sudhindra Baliga ◽  
AK Munshi
Keyword(s):  
Phosphoric Acid ◽  
Sem Analysis ◽  
Enamel Surface ◽  
Electron Microscopic ◽  
Etching Pattern ◽  
Significant Difference ◽  
Scanning Electron Microscopic ◽  
Group 2 ◽  
Scanning Electron ◽  
Group 1

Objectives: This study was undertaken to evaluate the topographical features of enamel surface deproteinized with sodium hypochlorite (NaOCl) and etched with phosphoric acid (H3PO4) compared to phosphoric acid alone using Scanning Electron Microscopic (SEM) Analysis. Study Design: 30 enamel blocks of 1mm2 from ten human sound extracted permanent molars were obtained and treated as under: Group 1 (10 blocks): Enamel surface was etched with 37% H3PO4 gel for 15 seconds. Group 2 (10 blocks):Enamel surface was treated with 5.25% NaOCl for 60 seconds and then etched with 37% H3PO4 gel for 15 seconds. 10 enamel blocks were included in the control group where no treatment was carried out. The samples were subjected to SEM analysis and 5 microphotographs of each sample were obtained at 500X magnification and evaluated for the quality of etching pattern of the enamel in percentage (%) using Auto-CAD 2007 software. Results: Mean values of etching pattern in Group 1 being 55.76% and Group 2 being 53.58%. No significant difference was observed between the two groups. Conclusion: The use of 37% phosphoric acid for 15 seconds still remains the best method for pretreatment of enamel.

scholarly journals SEM analysis of the acid-etched enamel patterns promoted by acidic monomers and phosphoric acids

2006 ◽  
Vol 14 (6) ◽  
pp. 427-435 ◽  
Author(s):  
Mirela Sanae Shinohara ◽  
Marcelo Tavares de Oliveira ◽  
Vinícius Di Hipólito ◽  
Marcelo Giannini ◽  
Mario Fernando de Goes
Keyword(s):  
Phosphoric Acid ◽  
Dental Enamel ◽  
Sem Analysis ◽  
Enamel Surface ◽  
The Other ◽  
Third Molars ◽  
Etching Pattern ◽  
Bonding Systems ◽  
Scanning Electron ◽  
Phosphoric Acids

ABSTRACT OBJECTIVE: Although self-etching bonding systems (SES) are indicated to prepare dental enamel for bonding, concerns have been expressed regarding their effectiveness. The aim of this study was to analyze the etching pattern (EP) of nine SES in comparison with 35% and 34% phosphoric acid etchants (FA) on intact (IN) and ground (GR) enamel surface. MATERIALS AND METHODS: Twenty-two human third molars were sectioned in mesial-distal and buccal-lingual directions, and four dental fragments were obtained from each tooth. Half of the fragments were ground using 600-grit SiC paper and the other half remained intact. The fragments were randomly assigned into 22 groups, according to the texture of enamel surface (IN and GR) and the technique to etch the enamel (34% FA, 35% FA, AdheSE primer; Brush & Bond; Clearfil Protect Bond primer; iBond; One-up Bond F; OptiBond Solo Plus primer; Tyrian SPE primer; Unifil Bond primer and Xeno III). Conditioners were applied to IN and GR enamel surfaces, according to the manufacturer's instructions. Specimens etched with phosphoric acids were washed with water, while the surfaces treated with SES were submitted to alternate rinsing with alcohol and acetone. The specimens were dried, sputter-coated and examined under a scanning electron microscope. RESULTS: For both IN and GR enamel surfaces, the EP of 34 and 35% FA was deeper and more homogeneous in comparison to EP of SES, except for Tyrian SPE. The acidic monomer action of self-etching systems was more effective on GR enamel. CONCLUSION: Most of the SES are less aggressive than phosphoric acid etchants and their etching effects were reduced on intact enamel surfaces.

Scanning electron microscopy evaluation of the effect of etching agents on human enamel surface

2006 ◽  
Vol 30 (3) ◽  
pp. 247-250 ◽  
Author(s):  
Caio Zanet ◽  
Victor Arana-Chavez ◽  
Marcelo Fava
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Phosphoric Acid ◽  
Enamel Surface ◽  
Human Enamel ◽  
Group 3 ◽  
Group 2 ◽  
Scanning Electron ◽  
Adhesive Materials ◽  
Group 1

Acid etching promotes microporosities on enamel surface, which provide a better bonding surface to adhesive materials. The purpose of this study was to comparatively analyze the microstructure of enamel surface after etching with 37% phosphoric acid or with two self-etching primers, Non-rinse conditioner (NRC) and Clearfil SE Bond (CSEB) using scanning electron microscopy. Thirty sound premolars were divided into 3 groups with ten teeth each: Group 1: the buccal surface was etched with 37% phosphoric acid for 15 seconds; Group 2: the buccal surface was etched with NRC for 20 seconds; Group 3: the buccal surface was etched with CSEB for 20 seconds. Teeth from Group 1 were rinsed with water; teeth from all groups were air-dried for 15 seconds. After that, all specimens were processed for scanning electron microscopy and analyzed in a Jeol 6100 SEM. The results showed deeper etching when the enamel surface was etched with 37% phosphoric acid, followed by NRC and CSEB. It is concluded that 37% phosphoric acid is still the best agent for a most effective enamel etching.

scholarly journals Scanning Electron Microscopy Analysis of the Anterior Capsulotomy Edge: A Comparative Study between Femtosecond Laser-Assisted Capsulotomy and Manual Capsulorhexis

2018 ◽  
Vol 2018 ◽  
pp. 1-6 ◽  
Author(s):  
Daniele Tognetto ◽  
Chiara De Giacinto ◽  
Alberto Armando Perrotta ◽  
Tommaso Candian ◽  
Alessandro Bova ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Femtosecond Laser ◽  
Comparative Study ◽  
Surface Irregularity ◽  
Significant Difference ◽  
Group 2 ◽  
Cut Surface ◽  
Scanning Electron ◽  
Group 1

Purpose. To compare the capsule edges ultrastructure obtained by two femtosecond laser-assisted cataract surgery (FLACS) platforms and manual continuous curvilinear capsulorhexis (CCC) using scanning electron microscopy (SEM). Setting. Eye Clinic, University of Trieste, Italy. Design. Experimental comparative study. Methods. 150 anterior capsules were collected and divided into three groups as follows: Group 1 (50 capsules) obtained with manual CCC, Groups 2 and 3 (each with 50 capsules) obtained with the Catalys Laser and the LenSx Laser, respectively. All samples were imaged by means of SEM and regularity of the cut surface, and thickness of the capsule edge were evaluated and compared. Results. All femtosecond laser (FSL) capsules were perfectly circular, whereas some alteration of the circular shape was observed in the manual ones. Group 1 showed a smooth and regular capsule edge without any surface irregularity, conversely Groups 2 and 3 showed postage-stamp perforations on the capsule edge. The cut surface irregularity value in Group 2 was 1.4 ± 0.63, while it was 0.7 ± 0.49 in Group 3 (p<0.05). Group 1 had a significantly lower thickness of the capsule edge than the FSL groups (p<0.05). No statistically significant difference in the capsule edge thickness between the FSL groups was found (p=0.244). Conclusions. Despite the presence of slight cut surface irregularities, both FSL capsulotomies showed a better geometry and circularity than the manual ones. Capsulotomy specimens obtained using both FSL capsulotomies showed laser-induced alterations of the capsule edge when compared with smooth and regular edges obtained using manual CCC.

scholarly journals Comparative parasitological and electron microscopic studies on the effects of Nitazoxanide and Praziquantel in Schistosoma mansoni-infected mice

2016 ◽  
Vol 4 (2) ◽  
pp. 228
Author(s):  
Hend A. El-Taweel ◽  
Mona H. El-Sayad ◽  
Sahar A. Abu Helw ◽  
Mohammad A. Al-Kazzaz
Keyword(s):  
Schistosoma Mansoni ◽  
Control Group ◽  
Electron Microscopic ◽  
Male And Female ◽  
Scanning Electron Microscopic ◽  
Antischistosomal Activity ◽  
Microscopic Studies ◽  
Group 3 ◽  
Group 2 ◽  
Group 1

This study was designed to evaluate antischistosomal activity of Nitazoxanide (NTZ) in Schistosoma mansoni-infected mice compared to Praziquantel (PZQ). Fifty four infected mice were recruited into 3 groups, each of 18 mice. Group 1 was infected non-treated control. Group 2 was infected and then treated with PZQ 500 mg for two days, and group 3 was infected and treated with NTZ 100 mg/kg for seven days. Efficacy of drugs was assessed by Parasitological, and scanning electron microscopic studies. PZQ reduced (4.9%, 22.5% and 50.7%) of faecal eggs, (22%, 22.6% and 55.1%) of intestinal eggs, (20.4%, 44.3% and 46.7%) of hepatic egg counts and (27%, 45.1% and 64.9%) of total worm load whereas, NTZ reduced (4.9%, 22.5% and 50.7%),of faecal eggs, (22%, 22.6% and 55.1%) of intestinal eggs ,(20.4%, 44.3% and 46.7%) of hepatic egg counts and (27%, 45.1% and 64.9%) of total worm load at 1, 2 and 4 WPT, respectively. The percentages of dead eggs were more than 80% after PZQ treatment and only 30% after NTZ at 4 WPT. PZQ showed extensive tegumental damages in male and female worms more than NTZ at 2 WPT. Our findings concluded that Nitazoxanide showed weaker antischistosomal activity in animal models than praziquantel.

scholarly journals Evaluation of sealing between abutment and inner connection of cone morse dental implant

2018 ◽  
Author(s):  
Derivaldo Moura Gois Filho ◽  
Vanessa Tavares de Gois-Santos ◽  
Ronaldo Santos Silva ◽  
Antônio Carlos Marqueti ◽  
Arthur Rodriguez Gonzalez Cortes ◽  
...  
Keyword(s):  
Dental Implants ◽  
Treatment Success ◽  
In Vitro Study ◽  
Study Results ◽  
Significant Difference ◽  
Group 2 ◽  
Dental Abutments ◽  
Scanning Electron ◽  
Group 1

Introduction: The adaptation of prostheses fixed over implants involves biomechanical aspects that are directly associated with treatment success. Objective: The aim of this in vitro study was to evaluate the presence of microgaps in the abutment/inner connection interface of cone morse dental implants. Materials and methods: Two groups of implants were analyzed. The first group (n = 16) employed single-manufacturer dental implants and abutments, whereas the second group (n = 16) combined multi-manufacturer materials. The sets were analyzed through scanning electron mi­croscopy, wherein microgaps between the implant connection and the abutment were observed. Results: Group 1 had an average microgap of 5.69 μm (SD ± 8.46 μm). Group 2 had an average microgap of 1.24 μm (SD ± 0.44 μm). A significant difference was found between the two groups (p = 0.002). Conclusion: Within the limitations of this study, results suggest that the group formed by multi-manufacturer implants and abutments (group 2) had smaller microgap values, and, therefore, a higher in vitro adaptation of components. DESCRIPTORS | Dental Implants; Dental Abutments; Scanning Electron Microscopy.

scholarly journals Efficacy of Two Irrigants Used with Self-Adjusting File System on Smear Layer: A Scanning Electron Microscopy Study

2014 ◽  
Vol 2014 ◽  
pp. 1-5 ◽  
Author(s):  
Özgür Genç Şen ◽  
Sadullah Kaya ◽  
Özgür Er ◽  
Tayfun Alaçam
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Citric Acid ◽  
Evaluation System ◽  
Smear Layer ◽  
Root Canals ◽  
Significant Difference ◽  
Group 2 ◽  
Scanning Electron ◽  
Group 1

Mechanical instrumentation of root canals produces a smear layer that adversely affects the root canal seal. The aim of this study was to evaluate efficacy of MTAD and citric acid solutions used with self-adjusting file (SAF) system on smear layer. Twenty-three single-rooted human teeth were used for the study. Canals were instrumented manually up to a number 20 K file size. SAF was used to prepare the root canals. The following groups were studied: Group 1: MTAD + 5.25% NaOCl, Group 2: 20% citric acid + 5.25% NaOCl, and Group 3: Control (5.25% NaOCl). All roots were split longitudinally and subjected to scanning electron microscopy. The presence of smear layer in the coronal, middle, and apical thirds was evaluated using a five-score evaluation system. Kruskal-Wallis and Mann-Whitney U tests were used for statistical analysis. In the coronal third, Group 2 exhibited the best results and was statistically different froms the other groups (P<0.05). There was not a significant difference among the three thirds of groups according to in-group comparisons (P>0.05). The solutions used in Group 1 and 2 could effectively remove smear layer in most of the specimens. However, citric acid was more effective than MTAD in the three thirds of the canal.

scholarly journals Scanning Electron Microscopic Analysis to Compare the Cleaning Efficiency of Three Different Irrigation Systems at Different Root Canal Levels: An in vitro Study

2014 ◽  
Vol 15 (4) ◽  
pp. 433-437 ◽  
Author(s):  
Varsha H Tambe ◽  
Jayshree Vishwas ◽  
WN Ghonmode ◽  
Pradnya Nagmode ◽  
Gaurav Pralhad Agrawal ◽  
...  
Keyword(s):  
Root Canal ◽  
Microscopic Analysis ◽  
Cleaning Efficiency ◽  
Electron Microscopic ◽  
Mean Values ◽  
Significant Difference ◽  
Scanning Electron Microscopic ◽  
Scanning Electron Microscopic Analysis ◽  
Scanning Electron

ABSTRACT Aim This study compared the efficacy of conventional, endovac and ultrasonic irrigation system for the removal of debris from root canal walls, using scanning electron microscopy (SEM) at cervical, middle and apical 3rd. Materials and methods A total of 30 freshly extracted human mandibular premolars with complete root formation were selected and divided into group 1 endovac, group 2 conventional and group 3 ultrasonic. After instrumentation and irrigation, the teeth were sectioned in buccolingual direction and analyzed by SEM and the results were analyzed statistically by students unpaired ‘t’ test. Results There was significant difference between mean values of cervical (CV), middle (M), and apical (A) when endovac compared with conventional and conventional compared with ultrasonic group (i.e. < 0.05) and no significant difference between mean values at CV, M and A when endovac compared with ultrasonic group. Conclusion Among all groups ultrasonic and endovac group showed cleaner canal walls and less amount of debris than conventional group. Clinical significance Application of ultrasonic and endovac can be used effectively for irrigation of canals leading to least debris and better prognosis. How to cite this article Tambe VH, Vishwas J, Ghonmode WN, Nagmode P, Agrawal GP, Balsaraf O. Scanning Electron Microscopic Analysis to Compare the Cleaning Efficiency of Three Different Irrigation Systems at Different Root Canal Levels: An in vitro Study. J Contemp Dent Pract 2014;15(4): 433-437.

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