<p class="western" align="justify"><span style="font-family: Arial, serif;"><span><span lang="en-US"><strong>O</strong></span></span></span><span style="font-family: Arial, serif;"><span><span lang="en-US"><strong>bjective: </strong></span></span></span><span style="font-family: Arial, serif;"><span><span lang="en-US">In this study, the expression of the extracellular matrix proteins was immunohistochemically studied and compared with the histological grading of squamous cell carcinomas of the lower lip and tongue. </span></span></span><span style="font-family: Arial, serif;"><span><span lang="en-US"><strong>Material and M</strong></span></span></span><span style="font-family: Arial, serif;"><span><span lang="en-US"><strong>ethods: </strong></span></span></span><span style="font-family: Arial, serif;"><span><span lang="en-US">The lower lip carcinomas (n=12) and the tongue carcinomas (n=12) were histopathologically graduated according to Bryne’s method. The immunohistochemical technique utilized specific antibodies to collagen IV and laminin. Histopathologic and immunohistochemical analysis were carried-out on the tumoral invasive front. </span></span></span><span style="font-family: Arial, serif;"><span><span lang="en-US"><strong>R</strong></span></span></span><span style="font-family: Arial, serif;"><span><span lang="en-US"><strong>esults: </strong></span></span></span><span style="font-family: Arial, serif;"><span><span lang="en-US">Most of lower lip carcinomas (91.7%) was classified in lower score and all tongue carcinomas (100%) in high score malignant grade (p<0.01). Collagen type IV expression was absent in the peritumoral basement membrane in 50% of lower lip carcinomas and in 66.7% of tongue carcinomas (p=0.09). Laminin expression was absent in the peritumoral basement membrane in 66.7% of lower lip carcinomas and in 58.3% of tongue carcinomas (p=0.48). When these two glicoproteins were expressed, they showed a linear, thin and discontinuous pattern and a weak intensity of expression. </span></span></span><span style="font-family: Arial, serif;"><span><span lang="en-US"><strong>C</strong></span></span></span><span style="font-family: Arial, serif;"><span><span lang="en-US"><strong>onclusion: </strong></span></span></span><span style="font-family: Arial, serif;"><span><span lang="en-US">The high score malignancy grade of the tongue carcinomas associated with the expression pattern of the studied matrix proteins. It suggests that tongue squamous cell carcinomas have more invasive potential and more aggressive biological behavior than the lower lip carcinomas.</span></span></span></p><p class="western" lang="en-US" align="justify"> </p><p class="western" align="justify"><span style="font-family: Arial, serif;"><span><span lang="en-US"><strong>Keywords</strong></span></span></span></p><p class="western" align="justify"><span style="font-family: Arial, serif;"><span><span lang="en-US">C</span></span></span><span style="font-family: Arial, serif;"><span><span lang="en-US">ollagen type IV; Laminin; </span></span></span><span style="font-family: Arial, serif;"><span><span lang="en-US">C</span></span></span><span style="font-family: Arial, serif;"><span><span lang="en-US">arcinoma; </span></span></span><span style="font-family: Arial, serif;"><span><span lang="en-US">I</span></span></span><span style="font-family: Arial, serif;"><span><span lang="en-US">mmunohistochemistry.</span></span></span></p><p> </p>
The correlation between HIF-1 alpha and VEGF in oral squamous cell carcinomas: Expression patterns and quantitative immunohistochemical analysis